Cell Lines in Research
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Questions and Answers

What is the purpose of giving codes or designations to cell lines?

For identification

Why is it important to ensure that each cell line designation is unique?

To avoid confusion in literature reports

What should be prefixed to the cell line designation at the time of publication?

A code designating the laboratory

Why are non-human cell lines generally preferred over human cell lines?

<p>Less risk of biohazards</p> Signup and view all the answers

Why do some workers suggest the use of finite cell lines over continuous cell lines?

<p>To ensure the expression of the right and appropriate functions of the cells.</p> Signup and view all the answers

Why are transformed cells preferred over normal cells for cell cultures?

<p>Transformed cells are immortalized and grow rapidly.</p> Signup and view all the answers

What are some key growth characteristics that need to be considered when selecting cell lines?

<p>Population doubling time, ability to grow in suspension, saturation density, and cloning efficiency.</p> Signup and view all the answers

Why is it important to regularly examine cell morphology in cell cultures?

<p>To check the health status of the cells, ensure absence of contamination, and detect any complications.</p> Signup and view all the answers

What are finite cell lines?

<p>Finite cell lines are cell lines with limited culture life spans that divide 20 to 100 times before extinction.</p> Signup and view all the answers

How do continuous cell lines differ from finite cell lines?

<p>Continuous cell lines are immortal and tumorigenic, while finite cell lines have limited culture life spans.</p> Signup and view all the answers

What is the significance of split ratio in cell culture?

<p>Split ratio is the divisor of the dilution ratio of a cell culture at subculture.</p> Signup and view all the answers

Define passage number in cell culture.

<p>Passage number is the number of times that a culture has been sub-cultured.</p> Signup and view all the answers

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