12 Questions
What is the purpose of giving codes or designations to cell lines?
For identification
Why is it important to ensure that each cell line designation is unique?
To avoid confusion in literature reports
What should be prefixed to the cell line designation at the time of publication?
A code designating the laboratory
Why are non-human cell lines generally preferred over human cell lines?
Less risk of biohazards
Why do some workers suggest the use of finite cell lines over continuous cell lines?
To ensure the expression of the right and appropriate functions of the cells.
Why are transformed cells preferred over normal cells for cell cultures?
Transformed cells are immortalized and grow rapidly.
What are some key growth characteristics that need to be considered when selecting cell lines?
Population doubling time, ability to grow in suspension, saturation density, and cloning efficiency.
Why is it important to regularly examine cell morphology in cell cultures?
To check the health status of the cells, ensure absence of contamination, and detect any complications.
What are finite cell lines?
Finite cell lines are cell lines with limited culture life spans that divide 20 to 100 times before extinction.
How do continuous cell lines differ from finite cell lines?
Continuous cell lines are immortal and tumorigenic, while finite cell lines have limited culture life spans.
What is the significance of split ratio in cell culture?
Split ratio is the divisor of the dilution ratio of a cell culture at subculture.
Define passage number in cell culture.
Passage number is the number of times that a culture has been sub-cultured.
This quiz discusses the differences between continuous and finite cell lines, the preference for transformed cells due to their rapid growth, availability of cell lines, and the importance of growth characteristics in research.
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