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Questions and Answers
What distinguishes transformed cell lines from primary derived transient cell lines?
Which of the following is a characteristic of established cell lines?
What was the significance of the first cell line established by G Gey?
Which resource claims to list over 107,000 cell lines?
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What is one of the primary advantages of working with established cell lines?
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What process can lead to the identification of spontaneously mutated colonies in primary cultures?
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Why might cancerous or tumorigenic origins be a common source for cell lines?
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What is a common concern regarding the use of cell lines in research?
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Transformed cell lines are defined as those that show signs of ageing and do not proliferate indefinitely.
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Henrietta Lacks was a significant figure in cell biology due to her cell line being the first established in 1950.
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A primary culture is initiated from cells, tissues, or organs and is typically used in experiments for an extended period.
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Cell lines can be easily established from both normal and cancerous cells.
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Cellosaurus contains information on over 80,000 cell lines of mammalian origin.
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The immortal nature of cell lines makes them a reliable and uniform source for research.
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Spontaneous mutations leading to faster-growing colonies can be identified through localized trypsinization.
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Established cell lines originate directly from primary cell cultures, which are usually derived from healthy cells.
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Study Notes
Established Cell Culture
- Established cell cultures consist of cells that can be passaged indefinitely and are inherently transformed.
- These cells commonly originate from tumors or cancers and initiate from primary cell cultures.
- Primary cultures are derived directly from animal cells, tissues, or organs and are typically utilized in experiments within a few days.
- Cell lines begin with the passaging of primary cultures; those that show senescence are termed primary-derived transient cell lines.
- Transformed cell lines continue to grow without signs of aging; they have the ability to proliferate indefinitely.
Historical Context
- The first established cell line was derived by G. Gey from J.H. Medical College, originating from Henrietta Lacks, a middle-aged woman with cervical cancer.
- Although Lacks did not survive, her cell line continues to be passaged, demonstrating the potential longevity of established cell lines.
Advantages of Established Cell Lines
- Provide uniformity in samples, which is essential for reproducibility.
- Offer an unlimited supply of cells, bypassing ethical concerns associated with obtaining cells from living subjects.
- Lack heterogeneity, ensuring consistent experimental results.
Characteristics of Cell Lines
- Cell lines are permanently established cultures that proliferate indefinitely under appropriate conditions.
- Differ from cell strains by becoming immortalized, making them a reliable source for research and diagnostic assays.
- The first continuous cell line was established in 1950; advances have made it easier to obtain cell lines, particularly those from cancerous or tumorigenic tissue.
Methods of Isolation
- Spontaneous mutations can result in discovering a colony in primary cultures that appears un-aged and grows faster.
- These colonies can be isolated using localized trypsinization and replated in fresh culture dishes.
- Cloning procedures involve trypsinizing cells, diluting them, and plating singly in 96-well plates to form new colonies.
Induced Established Cell Lines
- Cellosaurus serves as a comprehensive knowledge resource for cell lines, containing over 107,000 entries, with about 80,000 from human sources.
- ATCC provides access to various mammalian cell lines for a fee, catering to research needs.
Quality Concerns
- Despite the widespread use of cell lines, they are under scrutiny for quality and consistency, which can impact research findings.
Established Cell Culture
- Established cell cultures consist of cells that can be passaged indefinitely and are inherently transformed.
- These cells commonly originate from tumors or cancers and initiate from primary cell cultures.
- Primary cultures are derived directly from animal cells, tissues, or organs and are typically utilized in experiments within a few days.
- Cell lines begin with the passaging of primary cultures; those that show senescence are termed primary-derived transient cell lines.
- Transformed cell lines continue to grow without signs of aging; they have the ability to proliferate indefinitely.
Historical Context
- The first established cell line was derived by G. Gey from J.H. Medical College, originating from Henrietta Lacks, a middle-aged woman with cervical cancer.
- Although Lacks did not survive, her cell line continues to be passaged, demonstrating the potential longevity of established cell lines.
Advantages of Established Cell Lines
- Provide uniformity in samples, which is essential for reproducibility.
- Offer an unlimited supply of cells, bypassing ethical concerns associated with obtaining cells from living subjects.
- Lack heterogeneity, ensuring consistent experimental results.
Characteristics of Cell Lines
- Cell lines are permanently established cultures that proliferate indefinitely under appropriate conditions.
- Differ from cell strains by becoming immortalized, making them a reliable source for research and diagnostic assays.
- The first continuous cell line was established in 1950; advances have made it easier to obtain cell lines, particularly those from cancerous or tumorigenic tissue.
Methods of Isolation
- Spontaneous mutations can result in discovering a colony in primary cultures that appears un-aged and grows faster.
- These colonies can be isolated using localized trypsinization and replated in fresh culture dishes.
- Cloning procedures involve trypsinizing cells, diluting them, and plating singly in 96-well plates to form new colonies.
Induced Established Cell Lines
- Cellosaurus serves as a comprehensive knowledge resource for cell lines, containing over 107,000 entries, with about 80,000 from human sources.
- ATCC provides access to various mammalian cell lines for a fee, catering to research needs.
Quality Concerns
- Despite the widespread use of cell lines, they are under scrutiny for quality and consistency, which can impact research findings.
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Description
This quiz explores the concepts of established cell cultures, including primary cultures and cell lines derived from tumors. Understand the significance of cell passaging and how cell lines can maintain indefinite growth. Ideal for students studying biotechnology and cell biology.
