Cell Culture Week 1 - Introduction
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Questions and Answers

Qual es un de le componentes del sanguine?

  • Celledas rubre (correct)
  • Fibra sanguinea
  • Nutrientes vitaminicos
  • Carbohidratos

    • Que es le principale differente inter media avec e sin serum?

  • Le quantitá de protein (correct)
  • Le temperatura de incubar
  • Le tipo de aminoacidos
  • Le nivel de anticolas

    • Qual de iste medias es considerate animal-free?

  • Media non definite
  • Media con serum bovine fetal
  • Media con proteinas non derivate de animal (correct)
  • Media con nutrient de vegetales

    • Qual es le caracteristic de un media definite?

    <p>Contene omnes le constituent conosce</p> Signup and view all the answers

    Que es le component survenite post coagulation del sanguine?

    <p>Serum</p> Signup and view all the answers

    Quale de le sequente es un amino acido essential?

    <p>Glutamina</p> Signup and view all the answers

    Quanti amino acidos essentiales es designate pro le culturo de cellulas mammalian?

    <p>10</p> Signup and view all the answers

    Quale carbohydrat es le plus commun in le media de culturo?

    <p>Glucose</p> Signup and view all the answers

    Qual es le principal objectivo del cultivo cellular mammalian?

    <p>Propagare e cultivar celulas in un environment artificial.</p> Signup and view all the answers

    Quale amino acido non essential es presente in le media pro cellulas?

    <p>Arginine</p> Signup and view all the answers

    Qual elemento es essencial in le media de cultivo cellular?

    <p>Oxygeno.</p> Signup and view all the answers

    Quotidie se tracta de le contaminatio in le laboratorio?

    <p>Durante le quatro ultime lectiones.</p> Signup and view all the answers

    Quale elemento es considerate un ion bulk?

    <p>Calcio</p> Signup and view all the answers

    Quale de le sequente amino acidos es unstable e debe esser addite justo avant le uso?

    <p>Glutamina</p> Signup and view all the answers

    Qual es le nomine del processus de conservation de celulas?

    <p>Cryopreservation.</p> Signup and view all the answers

    Quale nutrientia es obligatoria in le media de culturo pro le proliferation de cellulas?

    <p>Amino Acidos</p> Signup and view all the answers

    Qual es un componente crucial del media de cultivo cellular?

    <p>Substantias nutrienti.</p> Signup and view all the answers

    Quando ha lugar le recapitulation del media de cultivo cellular?

    <p>Durante le lectiones 8, 9, 10 e 11.</p> Signup and view all the answers

    Quale factor non determina le densitate maxima de cellulas in le media?

    <p>Concentration de vitamins</p> Signup and view all the answers

    Qual es un resultato de un ambiente non appropriato in vitro?

    <p>Mort de celulas.</p> Signup and view all the answers

    Quotidie se usa le sanguine pro side cellular in vivo?

    <p>Pro transporto de oxygeno e nutrientes.</p> Signup and view all the answers

    Quale componente es essenziale pro le crecimiento e proliferation de cellulas?

    <p>Vitamines</p> Signup and view all the answers

    Quale es le function principal de albumina in media de cultura cellular?

    <p>Furnir proteines</p> Signup and view all the answers

    Perque le uso de antibioticos in culture medias es recomendate a evitar?

    <p>Proque illos pote encourager organismos resistente</p> Signup and view all the answers

    Quale es le role de salin inorganic in media de cellula?

    <p>Retener le equilibrio osmotic</p> Signup and view all the answers

    Quale de le sequente es considerate como micronutriente in culture?

    <p>Elementos traçes</p> Signup and view all the answers

    Quale es le motiv de que le fenol red es addite in media de cultura?

    <p>Pro indicar le pH</p> Signup and view all the answers

    Quale de le sequente proteinas es frequentemente usate in media de cultura cellular?

    <p>Fibronectin</p> Signup and view all the answers

    Quo es le function del acidos fatty in le media de cultura sin serum?

    <p>Sustener le vitalitate cellular</p> Signup and view all the answers

    Quale es le function de un medium de cultura mammaliana?

    <p>Recrear le fluido extracellular</p> Signup and view all the answers

    Qual es un requiremente importante in un medium de cultura pro cellulas animal?

    <p>Temperatura de 37°C</p> Signup and view all the answers

    Quale ingredientes es considerate basico pro un medium de cultura cellular?

    <p>Carbohydratio, amino acidos, vitaminas</p> Signup and view all the answers

    Quale es le major reason pro le complexitate de un medium de cultura pro mammifero?

    <p>Le diversitate de tipos cellular in mammiferi</p> Signup and view all the answers

    Quale es un elemento necessario pro le equilibrio de osmolaritate in un medium de cultura?

    <p>Osmolaritate similaire a sangue human</p> Signup and view all the answers

    Quale es le pH ideale pro un medium de cultura pro cellulas animal?

    <p>7.2-7.4</p> Signup and view all the answers

    Quale componente supplementa le medium de cultura pro cellulas animal?

    <p>Serum o su replacements</p> Signup and view all the answers

    Quale tipo de cellulas differ de cellulas mammalian in structur e function?

    <p>Cellulas bacterial</p> Signup and view all the answers

    Study Notes

    Weekly Lecture Schedule

    •  A schedule of lectures and labs is presented, detailing the topics covered and dates.

    Lecture Module Introduction

    •  The first lecture introduces the module.

    Cell Culture Laboratory

    •  The second lecture covers lab layout, equipment, and materials for mammalian cell culture.

    Contamination Control

    •  Lectures 3, 4, and 5 focus on contamination control procedures in cell culture.

    Nutrient Uptake

    •  Lecture 6 and 7 cover nutrient uptake in cell culture.

    Cell Biology

    •  Lecture 8 delves into the biology of culture cells.

    Cell Culture Media

    •  Lectures 9 and 10 cover cell culture media specifics. 

    ### Lab Data Analysis

    •  Lab 3 involves analyzing data related to cell culture.

    ### Reading Week

    •  Week 8 denotes a reading week.

    Cell Growth and Maintenance

    •  Lectures 11 through 14 span various mechanisms of cell growth and maintenance.

    Immune Response and Bioassays

    •  Lecture 15 discusses innate immune responses, while lecture 16 covers adaptive immune responses and bioassays.

    Revision

    •  The final week includes a revision period for the material covered.

    Mammalian Cell Culture Media

    •  Overview of the topic introduction, main discussion and conclusion.

    Introduction

    •  Mammalian cell culture entails removing animal cells from the body and propagating them in a suitable artificial environment ex vivo.

    Creating In Vitro Environment

    •  The objective is to recreate the in vivo conditions within an artificial, in vitro environment.

    Nutrient Provision in Body

    •  In the biological system, nutrients are carried to the cells by blood, a feature recapitulated in cell culture media.

    Cellular and Microbial Differences

    • Animal and microbial cells, while functionally similar, have distinct structures, requiring different types of media.

    Complexity in Mammilian Cell Growth Medium

    • The complexity of mammalian cell culture medium arises from the multitude of cell types in the organism, each performing a unique function, producing and secreting various molecules that circulate in the extracellular fluid.

    Maintaining Mammalian Cell Culture Media Conditions

    • Maintaining ideal conditions for cell culture media involves maintaining precise values of isotonic conditions, pH, and temperature, with temperature closely matching body temperature.

    Critical Components of Animal Cell Culture Media

    •  Essential components such as inorganic salts, carbohydrates, amino acids, vitamins, fatty acids, peptides, and trace elements constitute the basis of animal cell culture media. 

    Serum Supplements

    •  Serum or serum replacements are commonly used to supplement the basic elements in the medium.

    Serum Composition and Origin

    • Blood, comprising white blood cells, red blood cells, platelets, and plasma, is the source of serum. Clotting components are removed, leaving the serum.  Fetal bovine serum is a prevalent choice for in vitro cell culture.

    Different Categories of Media

    •  Different types of media include serum, serum-free media, animal-free media, and low-protein media.

    Defined vs. Non-Defined Media

    •  Defined media contains compounds with known concentrations, while non-defined media comprises a mixture of components with unspecified concentrations.

    Essential Amino Acids

    • Glutamine, isoleucine, leucine, lysine, phenylalanine, threonine, tryptophan, valine, methionine, and histidine are essential amino acids.

    Nonessential Amino Acids

    • Includes alanine, arginine, asparagine, aspartic acid, glutamic acid, glycine, proline, serine, tyrosine.

    Defining Components

    •  Amino acids are essential for protein synthesis, carbohydrates as the source of energy and proteins and peptides, fatty acids and lipids, vitamins, and inorganic salts contribute various roles in the culture.

    Trace Elements

    • Essential micronutrients for various biological processes, often originating from serum and hence necessary additions in serum-free media.

    pH Indicator

    • Phenol red, a pH indicator with no nutritive value, aids in monitoring proper pH values in the media.

    Bacterial Contamination and Antibiotic Use

    • Antibiotic use in culture media can both prevent and encourage certain contamination issues, including antibiotic resistance and masking of low-level contaminants.

    Conclusion

    •  Successful mammalian cell culture in vitro necessitates a balanced mixture of nutrients, including amino acids, monosaccharides, vitamins, inorganic ions, trace elements, additional factors such as hormones, growth factors, and attachment factors. Phenol red is used for monitoring the pH.  It is not advisable to routinely use antibiotics in the culture media.

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    Lecture 9: Mammalian Cell Culture Media PDF

    Description

    This quiz covers the introduction to the cell culture module, including the lab layout and essential equipment for mammalian cell culture. Additionally, it reviews the importance of contamination control procedures and nutrient uptake in cell culture. Test your knowledge on these vital topics in cell biology.

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