Podcast
Questions and Answers
Qual es un de le componentes del sanguine?
Qual es un de le componentes del sanguine?
- Celledas rubre (correct)
- Fibra sanguinea
- Nutrientes vitaminicos
- Carbohidratos
Que es le principale differente inter media avec e sin serum?
Que es le principale differente inter media avec e sin serum?
- Le quantitá de protein (correct)
- Le temperatura de incubar
- Le tipo de aminoacidos
- Le nivel de anticolas
Qual de iste medias es considerate animal-free?
Qual de iste medias es considerate animal-free?
- Media non definite
- Media con serum bovine fetal
- Media con proteinas non derivate de animal (correct)
- Media con nutrient de vegetales
Qual es le caracteristic de un media definite?
Qual es le caracteristic de un media definite?
Que es le component survenite post coagulation del sanguine?
Que es le component survenite post coagulation del sanguine?
Quale de le sequente es un amino acido essential?
Quale de le sequente es un amino acido essential?
Quanti amino acidos essentiales es designate pro le culturo de cellulas mammalian?
Quanti amino acidos essentiales es designate pro le culturo de cellulas mammalian?
Quale carbohydrat es le plus commun in le media de culturo?
Quale carbohydrat es le plus commun in le media de culturo?
Qual es le principal objectivo del cultivo cellular mammalian?
Qual es le principal objectivo del cultivo cellular mammalian?
Quale amino acido non essential es presente in le media pro cellulas?
Quale amino acido non essential es presente in le media pro cellulas?
Qual elemento es essencial in le media de cultivo cellular?
Qual elemento es essencial in le media de cultivo cellular?
Quotidie se tracta de le contaminatio in le laboratorio?
Quotidie se tracta de le contaminatio in le laboratorio?
Quale elemento es considerate un ion bulk?
Quale elemento es considerate un ion bulk?
Quale de le sequente amino acidos es unstable e debe esser addite justo avant le uso?
Quale de le sequente amino acidos es unstable e debe esser addite justo avant le uso?
Qual es le nomine del processus de conservation de celulas?
Qual es le nomine del processus de conservation de celulas?
Quale nutrientia es obligatoria in le media de culturo pro le proliferation de cellulas?
Quale nutrientia es obligatoria in le media de culturo pro le proliferation de cellulas?
Qual es un componente crucial del media de cultivo cellular?
Qual es un componente crucial del media de cultivo cellular?
Quando ha lugar le recapitulation del media de cultivo cellular?
Quando ha lugar le recapitulation del media de cultivo cellular?
Quale factor non determina le densitate maxima de cellulas in le media?
Quale factor non determina le densitate maxima de cellulas in le media?
Qual es un resultato de un ambiente non appropriato in vitro?
Qual es un resultato de un ambiente non appropriato in vitro?
Quotidie se usa le sanguine pro side cellular in vivo?
Quotidie se usa le sanguine pro side cellular in vivo?
Quale componente es essenziale pro le crecimiento e proliferation de cellulas?
Quale componente es essenziale pro le crecimiento e proliferation de cellulas?
Quale es le function principal de albumina in media de cultura cellular?
Quale es le function principal de albumina in media de cultura cellular?
Perque le uso de antibioticos in culture medias es recomendate a evitar?
Perque le uso de antibioticos in culture medias es recomendate a evitar?
Quale es le role de salin inorganic in media de cellula?
Quale es le role de salin inorganic in media de cellula?
Quale de le sequente es considerate como micronutriente in culture?
Quale de le sequente es considerate como micronutriente in culture?
Quale es le motiv de que le fenol red es addite in media de cultura?
Quale es le motiv de que le fenol red es addite in media de cultura?
Quale de le sequente proteinas es frequentemente usate in media de cultura cellular?
Quale de le sequente proteinas es frequentemente usate in media de cultura cellular?
Quo es le function del acidos fatty in le media de cultura sin serum?
Quo es le function del acidos fatty in le media de cultura sin serum?
Quale es le function de un medium de cultura mammaliana?
Quale es le function de un medium de cultura mammaliana?
Qual es un requiremente importante in un medium de cultura pro cellulas animal?
Qual es un requiremente importante in un medium de cultura pro cellulas animal?
Quale ingredientes es considerate basico pro un medium de cultura cellular?
Quale ingredientes es considerate basico pro un medium de cultura cellular?
Quale es le major reason pro le complexitate de un medium de cultura pro mammifero?
Quale es le major reason pro le complexitate de un medium de cultura pro mammifero?
Quale es un elemento necessario pro le equilibrio de osmolaritate in un medium de cultura?
Quale es un elemento necessario pro le equilibrio de osmolaritate in un medium de cultura?
Quale es le pH ideale pro un medium de cultura pro cellulas animal?
Quale es le pH ideale pro un medium de cultura pro cellulas animal?
Quale componente supplementa le medium de cultura pro cellulas animal?
Quale componente supplementa le medium de cultura pro cellulas animal?
Quale tipo de cellulas differ de cellulas mammalian in structur e function?
Quale tipo de cellulas differ de cellulas mammalian in structur e function?
Flashcards
Fetal Bovine Serum
Fetal Bovine Serum
Fetal bovine serum es un supplement de serum usate in le cultura de cellulas in vitro. Il es abundante in factores de crescimento, e basse in anticorpores.
Plasma
Plasma
Le component principal de sanguine que contine un miscela de proteinas, lipides, e carbohydratos.
Media Definide
Media Definide
Media de cultura cellular que contine componentes identificate.
Media Non-Definide
Media Non-Definide
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Media que contine serum
Media que contine serum
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Cultura cellular mammalian
Cultura cellular mammalian
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Medio de cultura cellular mammalian
Medio de cultura cellular mammalian
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In vivo
In vivo
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In vitro
In vitro
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Medio de cultura cellular
Medio de cultura cellular
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Cultivar cellulas
Cultivar cellulas
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Imitar le conditiones del corpo
Imitar le conditiones del corpo
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Componentes del medio de cultura cellular mammalian
Componentes del medio de cultura cellular mammalian
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Quid es le medio de cultura pro cellulas mammalian?
Quid es le medio de cultura pro cellulas mammalian?
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Per que le medio de cultura pro cellulas mammalian es si complexe?
Per que le medio de cultura pro cellulas mammalian es si complexe?
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Quale conditiones osmotic le cellulas mammalian require?
Quale conditiones osmotic le cellulas mammalian require?
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Qual es le temperatura optimal pro le cultura de cellulas mammalian?
Qual es le temperatura optimal pro le cultura de cellulas mammalian?
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Qual es le pH optimal pro le cultura de cellulas mammalian?
Qual es le pH optimal pro le cultura de cellulas mammalian?
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Quale nutrientes le cellulas mammalian require?
Quale nutrientes le cellulas mammalian require?
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Quale hormonios le cellulas mammalian require?
Quale hormonios le cellulas mammalian require?
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Quid es serum?
Quid es serum?
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Aminoacidos essential
Aminoacidos essential
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Aminoacidos non-essential
Aminoacidos non-essential
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Glutamina
Glutamina
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Aminoacidos
Aminoacidos
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Glucose
Glucose
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Carbohydrates
Carbohydrates
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Elementos trace
Elementos trace
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Elementos bulk
Elementos bulk
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Que proteinas e peptides es importante in medio de cultura cellular sin serum?
Que proteinas e peptides es importante in medio de cultura cellular sin serum?
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Que componentes es particularmente importante in medio de cultura cellular sin serum?
Que componentes es particularmente importante in medio de cultura cellular sin serum?
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Qual es le rolo de vitaminas in le medio de cultura cellular?
Qual es le rolo de vitaminas in le medio de cultura cellular?
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Qual es le rolo de sales inorganique in le medio de cultura cellular?
Qual es le rolo de sales inorganique in le medio de cultura cellular?
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Qual es le rolo de elementos de tracio in le medio de cultura cellular?
Qual es le rolo de elementos de tracio in le medio de cultura cellular?
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Qual es le function de phenol roge in le medio de cultura cellular?
Qual es le function de phenol roge in le medio de cultura cellular?
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Antibioticos esseva usate in le passato in medio de cultura cellular. Per que non es plus necessari usar los hodie?
Antibioticos esseva usate in le passato in medio de cultura cellular. Per que non es plus necessari usar los hodie?
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Qual es le desavantages del uso de antibioticos in le cultura de cellulas?
Qual es le desavantages del uso de antibioticos in le cultura de cellulas?
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Study Notes
Weekly Lecture Schedule
- Â A schedule of lectures and labs is presented, detailing the topics covered and dates.
Lecture Module Introduction
- Â The first lecture introduces the module.
Cell Culture Laboratory
- Â The second lecture covers lab layout, equipment, and materials for mammalian cell culture.
Contamination Control
- Â Lectures 3, 4, and 5 focus on contamination control procedures in cell culture.
Nutrient Uptake
- Â Lecture 6 and 7 cover nutrient uptake in cell culture.
Cell Biology
- Â Lecture 8 delves into the biology of culture cells.
Cell Culture Media
- Â Lectures 9 and 10 cover cell culture media specifics.Â
###Â Lab Data Analysis
- Â Lab 3 involves analyzing data related to cell culture.
###Â Reading Week
- Â Week 8 denotes a reading week.
Cell Growth and Maintenance
- Â Lectures 11 through 14 span various mechanisms of cell growth and maintenance.
Immune Response and Bioassays
- Â Lecture 15 discusses innate immune responses, while lecture 16 covers adaptive immune responses and bioassays.
Revision
- Â The final week includes a revision period for the material covered.
Mammalian Cell Culture Media
- Â Overview of the topic introduction, main discussion and conclusion.
Introduction
- Â Mammalian cell culture entails removing animal cells from the body and propagating them in a suitable artificial environment ex vivo.
Creating In Vitro Environment
- Â The objective is to recreate the in vivo conditions within an artificial, in vitro environment.
Nutrient Provision in Body
- Â In the biological system, nutrients are carried to the cells by blood, a feature recapitulated in cell culture media.
Cellular and Microbial Differences
- Animal and microbial cells, while functionally similar, have distinct structures, requiring different types of media.
Complexity in Mammilian Cell Growth Medium
- The complexity of mammalian cell culture medium arises from the multitude of cell types in the organism, each performing a unique function, producing and secreting various molecules that circulate in the extracellular fluid.
Maintaining Mammalian Cell Culture Media Conditions
- Maintaining ideal conditions for cell culture media involves maintaining precise values of isotonic conditions, pH, and temperature, with temperature closely matching body temperature.
Critical Components of Animal Cell Culture Media
- Â Essential components such as inorganic salts, carbohydrates, amino acids, vitamins, fatty acids, peptides, and trace elements constitute the basis of animal cell culture media.Â
Serum Supplements
- Â Serum or serum replacements are commonly used to supplement the basic elements in the medium.
Serum Composition and Origin
- Blood, comprising white blood cells, red blood cells, platelets, and plasma, is the source of serum. Clotting components are removed, leaving the serum. Fetal bovine serum is a prevalent choice for in vitro cell culture.
Different Categories of Media
- Â Different types of media include serum, serum-free media, animal-free media, and low-protein media.
Defined vs. Non-Defined Media
- Â Defined media contains compounds with known concentrations, while non-defined media comprises a mixture of components with unspecified concentrations.
Essential Amino Acids
- Glutamine, isoleucine, leucine, lysine, phenylalanine, threonine, tryptophan, valine, methionine, and histidine are essential amino acids.
Nonessential Amino Acids
- Includes alanine, arginine, asparagine, aspartic acid, glutamic acid, glycine, proline, serine, tyrosine.
Defining Components
- Â Amino acids are essential for protein synthesis, carbohydrates as the source of energy and proteins and peptides, fatty acids and lipids, vitamins, and inorganic salts contribute various roles in the culture.
Trace Elements
- Essential micronutrients for various biological processes, often originating from serum and hence necessary additions in serum-free media.
pH Indicator
- Phenol red, a pH indicator with no nutritive value, aids in monitoring proper pH values in the media.
Bacterial Contamination and Antibiotic Use
- Antibiotic use in culture media can both prevent and encourage certain contamination issues, including antibiotic resistance and masking of low-level contaminants.
Conclusion
-  Successful mammalian cell culture in vitro necessitates a balanced mixture of nutrients, including amino acids, monosaccharides, vitamins, inorganic ions, trace elements, additional factors such as hormones, growth factors, and attachment factors. Phenol red is used for monitoring the pH. It is not advisable to routinely use antibiotics in the culture media.
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Description
This quiz covers the introduction to the cell culture module, including the lab layout and essential equipment for mammalian cell culture. Additionally, it reviews the importance of contamination control procedures and nutrient uptake in cell culture. Test your knowledge on these vital topics in cell biology.
