Cell Culture Techniques and Safety

Laboratory techniques that enable the growth of cells in controlled environments.

A laboratory dedicated to cell culture, where researchers grow and maintain cells in various conditions.

Potential risks associated with cell culture, including exposure to infectious agents, toxic substances, and other hazards.

Model systems created using cell culture techniques to study biological processes, disease mechanisms, or drug effects.

The ability to manipulate genes and molecular pathways in cell cultures to study specific biological processes.

The use of cells from a single ancestor, ensuring all cells in the culture have the same genetic makeup.

Controlled environments that provide specific conditions for cell growth, eliminating variability from external factors.

The ability to generate consistent and reproducible data from cell culture experiments, due to the controlled environment.

Harmful microorganisms that can contaminate cell cultures, leading to changes in cell behavior and genetic makeup.

A common type of bacterial contamination, known for its rapid growth and diverse shapes.

A type of contamination caused by fungi with branching filaments that can be identified under a microscope.

A type of biological contaminant that can be difficult to detect, known for its small size and ability to interact with host cells.

The introduction of unwanted chemicals, such as plasticizers, into cell culture environments.

These guidelines define the recommended practices and procedures for maintaining a safe and sterile environment in a cell culture laboratory.

A set of rules and practices that dictate the safe handling of potentially hazardous biological materials, including pathogens.

The classification of microorganisms based on their potential risk to human health and the environment.

A microorganism that poses a low or moderate risk to laboratory workers and the community, with effective prophylaxis or treatment options available.

A microorganism that poses a high health risk and may lack effective treatment options upon infection, requiring specialized containment measures.

Levels of containment designed to limit the exposure of laboratory workers and the environment to hazardous biological agents.

A standard laboratory setting where general microbiological practices are followed.

A laboratory that requires trained personnel, restricted access, and additional safety measures to handle potentially infectious agents.

A laboratory designed to handle high-risk microorganisms with the highest level of containment, requiring specialized training and equipment.

Essential protection for laboratory workers, creating a physical barrier between them and potential hazards, such as infectious agents or hazardous chemicals.

A ventilated laboratory workstation that uses a HEPA filter to create a sterile environment, protecting both the worker and the cell culture.

A document that provides detailed information about the chemical and physical properties, storage, handling, disposal, and potential hazards of a specific laboratory reagent.

The absence of unwanted microorganisms, such as bacteria, viruses, fungi, and parasites, in a cell culture environment.

A dedicated space within a laboratory, free from other activities, specifically designed for cell culture work to maintain sterility and avoid contamination.

Tools and equipment used in a cell culture laboratory to promote sterility, accuracy, and efficiency in cell culture work.

A device that maintains a controlled humid environment, ideal for replicating the conditions inside the human body.

A microscope designed for observing cells in culture dishes, allowing researchers to assess their shape and count them.

A device that spins samples at high speeds, causing heavier components like cells to settle at the bottom for separation and collection.

A device that measures the acidity or alkalinity of solutions used in cell culture, crucial for maintaining the optimal pH for cell growth.

A method of sterilizing liquids by pushing them through a filter that traps bacteria and other microbes, allowing only sterile liquid to pass through.

A common practice in microbiology and cell culture where heat is used to kill microbes in instruments or materials.

The main sources of contamination in cell culture, potentially leading to problems in cell growth and research findings.

The ability to work with cell cultures without introducing contaminants, crucial for maintaining healthy and reliable cell growth.

The process of removing unwanted fluids or media from cell culture vessels, ensuring a clean and optimal environment for cells.

A type of contamination in cell cultures where fungal growth is visible, often leading to cell death and disrupting experiments.

A type of contamination in cell cultures where viruses infect cells, potentially altering their behavior and causing cellular damage.

The process of recognizing and eliminating contamination in cell cultures at the earliest signs, preventing further spread and saving time and resources.

The practice of consistently using aseptic techniques and careful handling of materials to minimize the risk of contamination, preventing unnecessary losses and ensuring accurate results.

A vital practice in cell culture to ensure proper identification, traceability, and safe handling of cell lines, ensuring they are correctly used and maintained.