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Questions and Answers
What level of protection does a Class II hood provide in cell culture procedures?
What level of protection does a Class II hood provide in cell culture procedures?
- Low level
- Medium level
- High level (correct)
- No protection
Which of the following is NOT a recommended good cell culture practice?
Which of the following is NOT a recommended good cell culture practice?
- Washing hands and wearing clean lab coat and gloves
- Using sterile glassware
- Disinfecting items in the laminar flow hood
- Handling multiple cell types simultaneously (correct)
How can Mycoplasma contamination be detected in cell cultures?
How can Mycoplasma contamination be detected in cell cultures?
- Detected by PCR, fluorescence staining, ELISA (correct)
- Causes a drop in pH of the culture
- Visible under a microscope
- Causes an increase in pH of the culture
What are the consequences of heavily infected yeast fungus culture?
What are the consequences of heavily infected yeast fungus culture?
Why is it advised not to talk, sing, or whistle during sterile procedures in cell culture?
Why is it advised not to talk, sing, or whistle during sterile procedures in cell culture?
What are the key components of good cell culture practices according to the text?
What are the key components of good cell culture practices according to the text?
What is the defining characteristic of organ culture compared to other types of cell cultures?
What is the defining characteristic of organ culture compared to other types of cell cultures?
Which type of culture maintains in vivo architecture and the differentiated character of cells?
Which type of culture maintains in vivo architecture and the differentiated character of cells?
What is a common feature of stem cell culture and differentiation processes?
What is a common feature of stem cell culture and differentiation processes?
In cell morphology, what term describes cells growing in a monolayer attached to the culture dish surface?
In cell morphology, what term describes cells growing in a monolayer attached to the culture dish surface?
Which factor is critical for maintaining good cell culture practices?
Which factor is critical for maintaining good cell culture practices?
What is a primary function of cell culture laboratory equipment in the maintenance of cultures?
What is a primary function of cell culture laboratory equipment in the maintenance of cultures?
Why is it important to freeze cells?
Why is it important to freeze cells?
What is the purpose of using a cryoprotective agent like DMSO or glycerol in the freezing medium?
What is the purpose of using a cryoprotective agent like DMSO or glycerol in the freezing medium?
At what temperature should cells be frozen to achieve a slow freezing rate?
At what temperature should cells be frozen to achieve a slow freezing rate?
How should one rapidly thaw cells that have been cryopreserved?
How should one rapidly thaw cells that have been cryopreserved?
Which piece of equipment is essential for providing an aseptic work area in a cell culture lab?
Which piece of equipment is essential for providing an aseptic work area in a cell culture lab?
What is the recommended temperature and CO2 level for cell culture incubators?
What is the recommended temperature and CO2 level for cell culture incubators?
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