18 Questions
What level of protection does a Class II hood provide in cell culture procedures?
High level
Which of the following is NOT a recommended good cell culture practice?
Handling multiple cell types simultaneously
How can Mycoplasma contamination be detected in cell cultures?
Detected by PCR, fluorescence staining, ELISA
What are the consequences of heavily infected yeast fungus culture?
Increase in pH
Why is it advised not to talk, sing, or whistle during sterile procedures in cell culture?
To avoid contamination by microorganisms in the environment
What are the key components of good cell culture practices according to the text?
Use of sterile reagents and glassware, disinfecting items in the laminar flow hood, and wearing clean lab coat and gloves
What is the defining characteristic of organ culture compared to other types of cell cultures?
Slow and limited cell proliferation
Which type of culture maintains in vivo architecture and the differentiated character of cells?
Histotypic culture
What is a common feature of stem cell culture and differentiation processes?
Maintenance of undifferentiated state
In cell morphology, what term describes cells growing in a monolayer attached to the culture dish surface?
Adherent cells
Which factor is critical for maintaining good cell culture practices?
Regular monitoring and subculturing
What is a primary function of cell culture laboratory equipment in the maintenance of cultures?
Controlling gas exchange
Why is it important to freeze cells?
To reduce risk of contamination
What is the purpose of using a cryoprotective agent like DMSO or glycerol in the freezing medium?
To maintain high viability during freezing
At what temperature should cells be frozen to achieve a slow freezing rate?
-80°C
How should one rapidly thaw cells that have been cryopreserved?
Quickly dilute in culture medium at 37°C
Which piece of equipment is essential for providing an aseptic work area in a cell culture lab?
Laminar flow hood
What is the recommended temperature and CO2 level for cell culture incubators?
Temperature: 37oC, CO2: 5%
Test your knowledge on biosafety cabinets used in cell culture procedures. Learn about the different classes of biosafety cabinets and good cell culture practices to prevent contamination.
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