Cell Culture Biosafety Cabinets Quiz

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What level of protection does a Class II hood provide in cell culture procedures?

Low level

Medium level

High level (correct)

No protection

Which of the following is NOT a recommended good cell culture practice?

Washing hands and wearing clean lab coat and gloves

Using sterile glassware

Disinfecting items in the laminar flow hood

Handling multiple cell types simultaneously (correct)

How can Mycoplasma contamination be detected in cell cultures?

Detected by PCR, fluorescence staining, ELISA (correct)

Causes a drop in pH of the culture

Visible under a microscope

Causes an increase in pH of the culture

What are the consequences of heavily infected yeast fungus culture?

Increase in pH (D) Signup and view all the answers

Why is it advised not to talk, sing, or whistle during sterile procedures in cell culture?

To avoid contamination by microorganisms in the environment (A) Signup and view all the answers

What are the key components of good cell culture practices according to the text?

Use of sterile reagents and glassware, disinfecting items in the laminar flow hood, and wearing clean lab coat and gloves (D) Signup and view all the answers

What is the defining characteristic of organ culture compared to other types of cell cultures?

Slow and limited cell proliferation (A) Signup and view all the answers

Which type of culture maintains in vivo architecture and the differentiated character of cells?

Histotypic culture (B) Signup and view all the answers

What is a common feature of stem cell culture and differentiation processes?

Maintenance of undifferentiated state (B) Signup and view all the answers

In cell morphology, what term describes cells growing in a monolayer attached to the culture dish surface?

Adherent cells (B) Signup and view all the answers

Which factor is critical for maintaining good cell culture practices?

Regular monitoring and subculturing (C) Signup and view all the answers

What is a primary function of cell culture laboratory equipment in the maintenance of cultures?

Controlling gas exchange (C) Signup and view all the answers

Why is it important to freeze cells?

To reduce risk of contamination (A) Signup and view all the answers

What is the purpose of using a cryoprotective agent like DMSO or glycerol in the freezing medium?

To maintain high viability during freezing (D) Signup and view all the answers

At what temperature should cells be frozen to achieve a slow freezing rate?

-80°C (D) Signup and view all the answers

How should one rapidly thaw cells that have been cryopreserved?

Quickly dilute in culture medium at 37°C (A) Signup and view all the answers

Which piece of equipment is essential for providing an aseptic work area in a cell culture lab?

Laminar flow hood (D) Signup and view all the answers

What is the recommended temperature and CO2 level for cell culture incubators?

Temperature: 37oC, CO2: 5% (A) Signup and view all the answers