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Cell Culture Quiz
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Cell Culture Quiz

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Questions and Answers

Which type of cell line has a finite life span in culture?

  • Transformed
  • Immortal
  • Continuous
  • Primary (correct)

    • What are the general medium requirements for cell growth?

  • Bulk ions, trace elements, sugars
  • Amino acids, vitamins, choline
  • Serum, antibiotics, CO2
  • All of the above (correct)

    • What does the red color of the culture medium indicate?

  • The presence of growth factors
  • The presence of phenol red
  • The presence of toxic nutrients
  • The correct pH for cell culture (correct)

    • Which type of microscope is used to visualize cultured cells?

    <p>Inverted microscope</p> Signup and view all the answers

    What is the advantage of using primary cells in experiments?

    <p>All of the above</p> Signup and view all the answers

    What is a disadvantage of using primary cells in experiments?

    <p>All of the above</p> Signup and view all the answers

    What is the main difference between primary cells and continuous cell lines?

    <p>Continuous cell lines have undergone immortalization</p> Signup and view all the answers

    Which enzyme causes bacteria expressing the lacZ gene to appear blue when grown on medium containing substrate analog X-gal or IPTG?

    <p>β-galactosidase</p> Signup and view all the answers

    What is the purpose of genetic engineering in creating genetically modified organisms (GMOs)?

    <p>To alter the genetic material of an organism using genetic engineering techniques</p> Signup and view all the answers

    What is the main difference between traditional breeding and genetic engineering?

    <p>Traditional breeding relies on natural processes, while genetic engineering involves deliberate manipulation of genes</p> Signup and view all the answers

    What is the purpose of Bt-modified organisms?

    <p>To produce protein crystals toxic to insects</p> Signup and view all the answers

    Which type of cell line has an abnormal and often transformed or immortal life span in culture?

    <p>Continuous cell line</p> Signup and view all the answers

    What is the purpose of using antibiotics in cell culture?

    <p>To control bacterial and fungal contaminants</p> Signup and view all the answers

    What are the general requirements for cell growth media?

    <p>Bulk ions, trace elements, sugars, amino acids, vitamins, choline, inositol, serum, and antibiotics</p> Signup and view all the answers

    What is the purpose of using serum in cell culture?

    <p>To promote healthy cell growth</p> Signup and view all the answers

    What is the function of a laminar flow hood in cell culture?

    <p>To provide a sterile work space</p> Signup and view all the answers

    What is the purpose of continuously flowing CO2-containing air through a cell culture incubator?

    <p>To promote healthy cell growth</p> Signup and view all the answers

    Which type of cell line has a limited growth potential even under optimal conditions?

    <p>Primary cells</p> Signup and view all the answers

    What is the advantage of using primary cells in experiments?

    <p>They avoid ethical objections raised against animal experiments</p> Signup and view all the answers

    What is the main difference between primary cells and continuous cell lines?

    <p>Primary cells have a limited lifespan and finite growth potential, while continuous cell lines have unlimited growth potential</p> Signup and view all the answers

    What is the purpose of genetic engineering in creating genetically modified organisms (GMOs)?

    <p>To introduce foreign DNA into eukaryotic cells</p> Signup and view all the answers

    What is a disadvantage of using primary cells in experiments?

    <p>They take more time to grow than other cell lines</p> Signup and view all the answers

    Which type of microscope is used to visualize cultured cells?

    <p>Inverted microscope</p> Signup and view all the answers

    Which enzyme is responsible for catalyzing the reaction with luciferin to produce light?

    <p>Luciferase</p> Signup and view all the answers

    What is the purpose of the lacZ gene in bacteria?

    <p>To cause bacteria to appear blue when grown on medium containing substrate analog X-gal or IPTG</p> Signup and view all the answers

    What is a transgenic organism?

    <p>An organism that has been genetically modified using genetic engineering techniques</p> Signup and view all the answers

    What is the main difference between traditional breeding and genetic engineering?

    <p>Traditional breeding involves the exchange of genetic material through sexual reproduction, while genetic engineering involves the direct manipulation of genes</p> Signup and view all the answers

    What is the purpose of the GFP gene in zebrafish?

    <p>To cause zebrafish to glow green under UV light</p> Signup and view all the answers

    What is the main advantage of using transgenic animals for the production of recombinant proteins?

    <p>Transgenic animals can produce proteins in a low-cost and renewable way</p> Signup and view all the answers

    What is the mechanism of action of Bt-modified organisms?

    <p>Bt-modified organisms produce Bt crystals that bind to receptors in the gut of insects, causing the gut wall to break down</p> Signup and view all the answers

    What is the purpose of the "golden rice" project?

    <p>To develop a genetically modified rice variety with improved vitamin A content</p> Signup and view all the answers

    Study Notes

    Cell Culture

    • Primary cells have a finite life span in culture.
    • General medium requirements for cell growth include a carbon source, energy source, and essential nutrients.
    • The red color of the culture medium indicates the presence of phenol red, a pH indicator.

    Microscopy

    • Inverted microscopes are used to visualize cultured cells.

    Genetic Engineering

    • The enzyme beta-galactosidase (lacZ) causes bacteria expressing the lacZ gene to appear blue when grown on medium containing substrate analog X-gal or IPTG.
    • The purpose of genetic engineering in creating genetically modified organisms (GMOs) is to introduce desirable traits or characteristics.
    • The main difference between traditional breeding and genetic engineering is the direct manipulation of genes vs. selective breeding.

    Cell Lines

    • Continuous cell lines have an abnormal and often transformed or immortal life span in culture.
    • Primary cells have a limited growth potential even under optimal conditions.

    Cell Culture Techniques

    • Antibiotics are used in cell culture to prevent bacterial contamination.
    • General requirements for cell growth media include a carbon source, energy source, and essential nutrients.
    • Serum is used in cell culture to provide growth factors and nutrients.
    • Laminar flow hoods are used in cell culture to maintain a sterile environment.
    • Continuously flowing CO2-containing air through a cell culture incubator maintains a stable pH and promotes cell growth.

    Biotechnology

    • The purpose of Bt-modified organisms is to produce a toxin that kills pests, reducing the need for pesticides.
    • The lacZ gene in bacteria is used as a reporter gene to indicate gene expression.
    • A transgenic organism is one whose genome has been altered by the introduction of foreign DNA.
    • The GFP gene in zebrafish is used as a marker gene to visualize gene expression.
    • The main advantage of using transgenic animals for the production of recombinant proteins is the ability to produce large quantities of protein in a cost-effective manner.
    • Bt-modified organisms work by producing a toxin that kills pests, reducing the need for pesticides.
    • The "golden rice" project aims to produce rice that contains beta-carotene, a precursor to vitamin A, to combat vitamin A deficiency.

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    Related Documents

    PPT3.pdf

    Description

    Test your knowledge on cell culture, a routine laboratory technique used for in vitro analysis of life processes. Learn about the culturing of cells derived from multicellular eukaryotes, including primary and continuous cell lines. Explore the methods for maintaining cells in culture, such as temperature and gas mixture requirements.

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