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Which type of cell line has a finite life span in culture?
Which type of cell line has a finite life span in culture?
What are the general medium requirements for cell growth?
What are the general medium requirements for cell growth?
What does the red color of the culture medium indicate?
What does the red color of the culture medium indicate?
Which type of microscope is used to visualize cultured cells?
Which type of microscope is used to visualize cultured cells?
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What is the advantage of using primary cells in experiments?
What is the advantage of using primary cells in experiments?
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What is a disadvantage of using primary cells in experiments?
What is a disadvantage of using primary cells in experiments?
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What is the main difference between primary cells and continuous cell lines?
What is the main difference between primary cells and continuous cell lines?
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Which enzyme causes bacteria expressing the lacZ gene to appear blue when grown on medium containing substrate analog X-gal or IPTG?
Which enzyme causes bacteria expressing the lacZ gene to appear blue when grown on medium containing substrate analog X-gal or IPTG?
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What is the purpose of genetic engineering in creating genetically modified organisms (GMOs)?
What is the purpose of genetic engineering in creating genetically modified organisms (GMOs)?
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What is the main difference between traditional breeding and genetic engineering?
What is the main difference between traditional breeding and genetic engineering?
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What is the purpose of Bt-modified organisms?
What is the purpose of Bt-modified organisms?
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Which type of cell line has an abnormal and often transformed or immortal life span in culture?
Which type of cell line has an abnormal and often transformed or immortal life span in culture?
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What is the purpose of using antibiotics in cell culture?
What is the purpose of using antibiotics in cell culture?
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What are the general requirements for cell growth media?
What are the general requirements for cell growth media?
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What is the purpose of using serum in cell culture?
What is the purpose of using serum in cell culture?
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What is the function of a laminar flow hood in cell culture?
What is the function of a laminar flow hood in cell culture?
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What is the purpose of continuously flowing CO2-containing air through a cell culture incubator?
What is the purpose of continuously flowing CO2-containing air through a cell culture incubator?
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Which type of cell line has a limited growth potential even under optimal conditions?
Which type of cell line has a limited growth potential even under optimal conditions?
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What is the advantage of using primary cells in experiments?
What is the advantage of using primary cells in experiments?
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What is the main difference between primary cells and continuous cell lines?
What is the main difference between primary cells and continuous cell lines?
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What is the purpose of genetic engineering in creating genetically modified organisms (GMOs)?
What is the purpose of genetic engineering in creating genetically modified organisms (GMOs)?
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What is a disadvantage of using primary cells in experiments?
What is a disadvantage of using primary cells in experiments?
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Which type of microscope is used to visualize cultured cells?
Which type of microscope is used to visualize cultured cells?
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Which enzyme is responsible for catalyzing the reaction with luciferin to produce light?
Which enzyme is responsible for catalyzing the reaction with luciferin to produce light?
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What is the purpose of the lacZ gene in bacteria?
What is the purpose of the lacZ gene in bacteria?
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What is a transgenic organism?
What is a transgenic organism?
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What is the main difference between traditional breeding and genetic engineering?
What is the main difference between traditional breeding and genetic engineering?
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What is the purpose of the GFP gene in zebrafish?
What is the purpose of the GFP gene in zebrafish?
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What is the main advantage of using transgenic animals for the production of recombinant proteins?
What is the main advantage of using transgenic animals for the production of recombinant proteins?
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What is the mechanism of action of Bt-modified organisms?
What is the mechanism of action of Bt-modified organisms?
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What is the purpose of the "golden rice" project?
What is the purpose of the "golden rice" project?
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Study Notes
Cell Culture
- Primary cells have a finite life span in culture.
- General medium requirements for cell growth include a carbon source, energy source, and essential nutrients.
- The red color of the culture medium indicates the presence of phenol red, a pH indicator.
Microscopy
- Inverted microscopes are used to visualize cultured cells.
Genetic Engineering
- The enzyme beta-galactosidase (lacZ) causes bacteria expressing the lacZ gene to appear blue when grown on medium containing substrate analog X-gal or IPTG.
- The purpose of genetic engineering in creating genetically modified organisms (GMOs) is to introduce desirable traits or characteristics.
- The main difference between traditional breeding and genetic engineering is the direct manipulation of genes vs. selective breeding.
Cell Lines
- Continuous cell lines have an abnormal and often transformed or immortal life span in culture.
- Primary cells have a limited growth potential even under optimal conditions.
Cell Culture Techniques
- Antibiotics are used in cell culture to prevent bacterial contamination.
- General requirements for cell growth media include a carbon source, energy source, and essential nutrients.
- Serum is used in cell culture to provide growth factors and nutrients.
- Laminar flow hoods are used in cell culture to maintain a sterile environment.
- Continuously flowing CO2-containing air through a cell culture incubator maintains a stable pH and promotes cell growth.
Biotechnology
- The purpose of Bt-modified organisms is to produce a toxin that kills pests, reducing the need for pesticides.
- The lacZ gene in bacteria is used as a reporter gene to indicate gene expression.
- A transgenic organism is one whose genome has been altered by the introduction of foreign DNA.
- The GFP gene in zebrafish is used as a marker gene to visualize gene expression.
- The main advantage of using transgenic animals for the production of recombinant proteins is the ability to produce large quantities of protein in a cost-effective manner.
- Bt-modified organisms work by producing a toxin that kills pests, reducing the need for pesticides.
- The "golden rice" project aims to produce rice that contains beta-carotene, a precursor to vitamin A, to combat vitamin A deficiency.
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Description
Test your knowledge on cell culture, a routine laboratory technique used for in vitro analysis of life processes. Learn about the culturing of cells derived from multicellular eukaryotes, including primary and continuous cell lines. Explore the methods for maintaining cells in culture, such as temperature and gas mixture requirements.
