20 Questions
Which type of cell culture has a finite life span?
Primary cells
What is the growth environment required for cell culture?
37C, 5% CO2
Which type of cell culture represents tissue most closely?
Primary cells
What are the general medium requirements for cell culture?
Bulk ions and trace elements
Which method is commonly used for cell culture transfection?
Electroporation
Which term is used to describe organisms with altered genetic material using engineering?
Transgenic organisms
What is the main difference between traditional breeding and genetic engineering?
Traditional breeding cannot alter the genetic material, while genetic engineering can
What is the purpose of using Bt-modified organisms?
To provide insecticidal effects without human toxicity
Which of the following is an example of a transgenic animal used for commercial production?
Recombinant protein in the milk of cows
What is the purpose of engineering Golden Rice?
To improve the nutritional content of rice
Which type of cell culture has the advantage of avoiding ethical objections and providing more relevant results than cell lines?
Primary cells
What is the main disadvantage of primary cells in cell culture?
Limited growth potential and eventual death
Which type of cells have inherent gene mutations that allow them to escape cell proliferation regulation processes?
Continuous cells
What is the goal of cell culture transfection?
Analysis of effect of DNA on cell function using reporter genes
Which method of cell culture transfection has the highest efficiency?
Electroporation
Which of the following is an example of a transgenic animal used for commercial production?
RhAT in transgenic goats
What is the purpose of engineering Golden Rice?
To produce beta-carotene, niacin, iron, essential minerals, etc
Which of the following is an insecticidal effect without conferring human toxicity?
Bt-modified organisms
What is the main difference between traditional breeding and genetic engineering?
Traditional breeding involves the transfer of genes that cannot be controlled, while genetic engineering involves the specific insertion of a gene
Which of the following fruits has been genetically modified to be resistant to mosaic virus?
Papaya
Test your knowledge on cell culture and in vitro analysis of multicellular eukaryotes with this quiz. Learn about the different types of cell cultures, their maintenance requirements, and the role of primary cells in research.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
