Cell Culture Quiz
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Questions and Answers

Which type of cell culture has a finite life span?

  • Stem cell lines
  • Primary cells (correct)
  • Continuous cells
  • Cell lines

    • What is the growth environment required for cell culture?

  • 37C, 5% CO2 (correct)
  • 25C, 5% CO2
  • 37C, 1% CO2
  • 25C, 1% CO2

    • Which type of cell culture represents tissue most closely?

  • Primary cells (correct)
  • Cell lines
  • Continuous cells
  • Stem cell lines

    • What are the general medium requirements for cell culture?

    <p>Bulk ions and trace elements</p> Signup and view all the answers

    Which method is commonly used for cell culture transfection?

    <p>Electroporation</p> Signup and view all the answers

    Which term is used to describe organisms with altered genetic material using engineering?

    <p>Transgenic organisms</p> Signup and view all the answers

    What is the main difference between traditional breeding and genetic engineering?

    <p>Traditional breeding cannot alter the genetic material, while genetic engineering can</p> Signup and view all the answers

    What is the purpose of using Bt-modified organisms?

    <p>To provide insecticidal effects without human toxicity</p> Signup and view all the answers

    Which of the following is an example of a transgenic animal used for commercial production?

    <p>Recombinant protein in the milk of cows</p> Signup and view all the answers

    What is the purpose of engineering Golden Rice?

    <p>To improve the nutritional content of rice</p> Signup and view all the answers

    Which type of cell culture has the advantage of avoiding ethical objections and providing more relevant results than cell lines?

    <p>Primary cells</p> Signup and view all the answers

    What is the main disadvantage of primary cells in cell culture?

    <p>Limited growth potential and eventual death</p> Signup and view all the answers

    Which type of cells have inherent gene mutations that allow them to escape cell proliferation regulation processes?

    <p>Continuous cells</p> Signup and view all the answers

    What is the goal of cell culture transfection?

    <p>Analysis of effect of DNA on cell function using reporter genes</p> Signup and view all the answers

    Which method of cell culture transfection has the highest efficiency?

    <p>Electroporation</p> Signup and view all the answers

    Which of the following is an example of a transgenic animal used for commercial production?

    <p>RhAT in transgenic goats</p> Signup and view all the answers

    What is the purpose of engineering Golden Rice?

    <p>To produce beta-carotene, niacin, iron, essential minerals, etc</p> Signup and view all the answers

    Which of the following is an insecticidal effect without conferring human toxicity?

    <p>Bt-modified organisms</p> Signup and view all the answers

    What is the main difference between traditional breeding and genetic engineering?

    <p>Traditional breeding involves the transfer of genes that cannot be controlled, while genetic engineering involves the specific insertion of a gene</p> Signup and view all the answers

    Which of the following fruits has been genetically modified to be resistant to mosaic virus?

    <p>Papaya</p> Signup and view all the answers

    Study Notes

    Cell Culture Types and Characteristics

    • Finite life span: Primary cell cultures have a limited life span and undergo senescence after a certain number of divisions.
    • Tissue representation: Organotypic cultures represent tissues more closely, maintaining the architecture and function of the original tissue.

    Growth Environment and Medium Requirements

    • Growth environment: Cell culture requires a sterile environment, controlled temperature, humidity, and specific atmospheric conditions, often supplemented with CO2.
    • Medium requirements: General culture media must contain essential nutrients, vitamins, amino acids, and appropriate pH levels for optimal cell growth.

    Transfection and Genetic Modification

    • Common transfection method: Lipofection is widely used for cell culture transfection, facilitating the introduction of nucleic acids into cells.
    • Genetically altered organisms: Organisms created through genetic engineering are referred to as transgenic organisms.

    Genetic Engineering vs. Traditional Breeding

    • Main difference: Genetic engineering involves the direct manipulation of an organism's DNA, while traditional breeding relies on selective breeding methods over generations.

    Applications of Genetic Engineering

    • Purpose of Bt-modified organisms: These organisms are engineered to express Bacillus thuringiensis (Bt) toxin, providing insecticidal effects without toxic impacts on humans.
    • Engineering Golden Rice: The main goal is to increase beta-carotene levels to combat vitamin A deficiency in populations reliant on rice as a staple food.

    Transgenic Animals and Commercial Production

    • Example of transgenic animal: Examples include goats engineered to produce human proteins in their milk.
    • Primary cells vs. cell lines: Primary cells provide more relevant physiological results but have a limited life span compared to established cell lines, which continue to proliferate indefinitely.

    Cell Proliferation and Regulation

    • Inherent gene mutations: Cancer cells are characterized by mutations that allow them to bypass cell proliferation regulation processes.
    • Goal of transfection: The primary aim of cell culture transfection is to introduce foreign nucleic acids into a cell to study gene function and expression.

    Efficient Transfection Methods

    • Highest efficiency method: Electroporation generally provides the highest efficiency among transfection methods, allowing for effective nucleic acid delivery into cells.

    Genetic Modification Examples

    • Mosaic virus resistant fruit: Watermelon has been genetically modified to be resistant to the mosaic virus, showcasing agricultural applications of genetic engineering.

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    Description

    Test your knowledge on cell culture and in vitro analysis of multicellular eukaryotes with this quiz. Learn about the different types of cell cultures, their maintenance requirements, and the role of primary cells in research.

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