10 Questions
What is the primary reason for adding DMSO as a dehydration agent in the cryopreservation process?
To prevent ice crystal formation, which can damage cells and cause cell death.
What is the purpose of Mr. Frosty with isopropanol in the cryopreservation process?
To decrease the temperature gradually, allowing for a slower cooling rate and reducing the risk of ice crystal formation.
What is the initial step in the cryopreservation process?
Remove the growth medium.
What is the purpose of trypsin-EDTA in the cryopreservation process?
To dislodge the cells.
What is the composition of the freezing medium used in the cryopreservation process?
10% DMSO in FBS.
What is the next step after incubating the cryovials at -80°C overnight?
Transfer the cryovials to Liquid nitrogen.
What is the initial step in the thawing process?
Vial from liquid nitrogen is placed into 37°C waterbath.
What is the purpose of adding 1 ml of complete culture medium after thawing the cells?
To help the cells recover and start growing.
What is the recommended frequency for changing the medium after thawing the cells?
Every 3 days at most.
What is the purpose of checking the cultures after 24 hours?
To ensure that they are attached to the plate.
Learn about the importance of pH levels in cell culture media, including the ideal pH range for cell growth and how HEPES helps maintain optimal conditions.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
