Podcast
Questions and Answers
What is the primary reason for adding DMSO as a dehydration agent in the cryopreservation process?
What is the primary reason for adding DMSO as a dehydration agent in the cryopreservation process?
To prevent ice crystal formation, which can damage cells and cause cell death.
What is the purpose of Mr. Frosty with isopropanol in the cryopreservation process?
What is the purpose of Mr. Frosty with isopropanol in the cryopreservation process?
To decrease the temperature gradually, allowing for a slower cooling rate and reducing the risk of ice crystal formation.
What is the initial step in the cryopreservation process?
What is the initial step in the cryopreservation process?
Remove the growth medium.
What is the purpose of trypsin-EDTA in the cryopreservation process?
What is the purpose of trypsin-EDTA in the cryopreservation process?
Signup and view all the answers
What is the composition of the freezing medium used in the cryopreservation process?
What is the composition of the freezing medium used in the cryopreservation process?
Signup and view all the answers
What is the next step after incubating the cryovials at -80°C overnight?
What is the next step after incubating the cryovials at -80°C overnight?
Signup and view all the answers
What is the initial step in the thawing process?
What is the initial step in the thawing process?
Signup and view all the answers
What is the purpose of adding 1 ml of complete culture medium after thawing the cells?
What is the purpose of adding 1 ml of complete culture medium after thawing the cells?
Signup and view all the answers
What is the recommended frequency for changing the medium after thawing the cells?
What is the recommended frequency for changing the medium after thawing the cells?
Signup and view all the answers
What is the purpose of checking the cultures after 24 hours?
What is the purpose of checking the cultures after 24 hours?
Signup and view all the answers
