10 Questions
What were the initial attempts to create culture media?
The initial attempts to create culture media were based on natural media derived from tissue extracts and body fluids such as chick embryo extract, serum, lymph, etc.
What medium became widely adopted for cell propagation?
Eagle’s Basal Medium/ Eagle’s Minimal Essential Medium (MEM) became widely adopted for cell propagation.
What type of medium may be required for the isolation and propagation of cells of a specific lineage?
The isolation and propagation of cells of a specific lineage may require a selective serum-free medium.
What were the succeeding developments aimed at in culture media?
The succeeding developments in culture media were aimed at replacing serum, optimizing media for different cell types, or modifying for specific conditions.
Which media are commonly used for cells grown for the formation of products, as hosts for viral propagation, or for non-cell-specific molecular studies?
Cells grown for the formation of products, as hosts for viral propagation, or for non-cell-specific molecular studies commonly rely on Eagle’s MEM, DMEM, and increasingly RPMI, supplemented with serum.
What are some examples of natural media used in early attempts to create culture media?
chick embryo extract, serum, lymph
What are some supplements that were added to Eagle’s Basal Medium/ Eagle’s Minimal Essential Medium (MEM)?
calf, human, or horse serum, protein hydrolysates, and embryo extract
What were the main goals of the succeeding developments in culture media?
replacing serum, optimizing media for different cell types, and modifying for specific conditions
What type of medium is commonly used for cells grown for the formation of products, as hosts for viral propagation, or for non-cell-specific molecular studies?
Eagle’s MEM, DMEM, and RPMI
What type of medium may be required for the isolation and propagation of cells of a specific lineage?
selective serum-free medium
Test your knowledge on culture media used in cell culture with this quiz. Learn about the evolution from natural media to defined media, including the widely adopted Eagle's Basal Medium/Minimal Essential Medium (MEM) and its various supplements.
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