Cell Culture Laboratory Safety

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What is the most common problem encountered in cell culture laboratories?

Biological contamination (correct)

Mycoplasma contamination

Chemical contamination

Cross contamination by other cell lines

What is the primary purpose of using a sterile pipette only once in cell culture laboratory?

To prevent chemical contamination

To prevent bacterial contamination (correct)

To avoid altering the pH of the culture medium

To reduce the risk of cross contamination

What is the size range of bacteria in diameters?

A few millimeters

A few centimeters

A few nanometers

A few micrometers (correct)

What is the purpose of wiping the area with 70% ethanol after mopping up spillage in a cell culture laboratory?

To disinfect the work area Signup and view all the answers

What are the two main categories of cell culture contaminants?

Biological and chemical contaminants Signup and view all the answers

Why is it important to follow good aseptic technique in cell culture laboratory?

To reduce the frequency and seriousness of biological contamination Signup and view all the answers

What is a common problem in cell cultures, with serious consequences?

Cross-contamination with HeLa and other fast growing cell lines Signup and view all the answers

What type of filter was used to obtain the images of the cell cultures?

450 ± 30 nm bandpass filter Signup and view all the answers

Why should antibiotics not be used routinely in cell culture?

All of the above Signup and view all the answers

What is the recommended practice to avoid cross-contamination of cell lines?

Obtaining cell lines from reputable cell banks and periodically checking the characteristics of the cell lines Signup and view all the answers

What analysis can confirm the presence or absence of cross-contamination in cell cultures?

DNA fingerprinting, karyotype analysis, and isotype analysis Signup and view all the answers

When should antibiotics be used in cell culture?

As a last resort and only for short term applications Signup and view all the answers

What is the primary goal of a biosafety program in a cell culture laboratory?

To reduce or eliminate exposure of laboratory workers and the environment to potentially harmful biological agents Signup and view all the answers

What is a common hazard associated with handling cells and tissues in a laboratory?

Accidental punctures with syringe needles Signup and view all the answers

What document provides regulations and recommendations for biosafety in the United States?

Biosafety in Microbiological and Biomedical Laboratories Signup and view all the answers

What is the most important element of safety in a cell culture laboratory?

Strict adherence to standard microbiological practices and techniques Signup and view all the answers

What is a potential route of exposure to infectious agents in a cell culture laboratory?

All of the above Signup and view all the answers

What type of substances are commonly handled in a cell culture laboratory?

Toxic, corrosive, or mutagenic solvents and reagents Signup and view all the answers

Cell Culture Laboratory Safety

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