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Questions and Answers
What is the most common problem encountered in cell culture laboratories?
What is the most common problem encountered in cell culture laboratories?
- Biological contamination (correct)
- Mycoplasma contamination
- Chemical contamination
- Cross contamination by other cell lines
What is the primary purpose of using a sterile pipette only once in cell culture laboratory?
What is the primary purpose of using a sterile pipette only once in cell culture laboratory?
- To prevent chemical contamination
- To prevent bacterial contamination (correct)
- To avoid altering the pH of the culture medium
- To reduce the risk of cross contamination
What is the size range of bacteria in diameters?
What is the size range of bacteria in diameters?
- A few millimeters
- A few centimeters
- A few nanometers
- A few micrometers (correct)
What is the purpose of wiping the area with 70% ethanol after mopping up spillage in a cell culture laboratory?
What is the purpose of wiping the area with 70% ethanol after mopping up spillage in a cell culture laboratory?
What are the two main categories of cell culture contaminants?
What are the two main categories of cell culture contaminants?
Why is it important to follow good aseptic technique in cell culture laboratory?
Why is it important to follow good aseptic technique in cell culture laboratory?
What is a common problem in cell cultures, with serious consequences?
What is a common problem in cell cultures, with serious consequences?
What type of filter was used to obtain the images of the cell cultures?
What type of filter was used to obtain the images of the cell cultures?
Why should antibiotics not be used routinely in cell culture?
Why should antibiotics not be used routinely in cell culture?
What is the recommended practice to avoid cross-contamination of cell lines?
What is the recommended practice to avoid cross-contamination of cell lines?
What analysis can confirm the presence or absence of cross-contamination in cell cultures?
What analysis can confirm the presence or absence of cross-contamination in cell cultures?
When should antibiotics be used in cell culture?
When should antibiotics be used in cell culture?
What is the primary goal of a biosafety program in a cell culture laboratory?
What is the primary goal of a biosafety program in a cell culture laboratory?
What is a common hazard associated with handling cells and tissues in a laboratory?
What is a common hazard associated with handling cells and tissues in a laboratory?
What document provides regulations and recommendations for biosafety in the United States?
What document provides regulations and recommendations for biosafety in the United States?
What is the most important element of safety in a cell culture laboratory?
What is the most important element of safety in a cell culture laboratory?
What is a potential route of exposure to infectious agents in a cell culture laboratory?
What is a potential route of exposure to infectious agents in a cell culture laboratory?
What type of substances are commonly handled in a cell culture laboratory?
What type of substances are commonly handled in a cell culture laboratory?
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