18 Questions
What is the most common problem encountered in cell culture laboratories?
Biological contamination
What is the primary purpose of using a sterile pipette only once in cell culture laboratory?
To prevent bacterial contamination
What is the size range of bacteria in diameters?
A few micrometers
What is the purpose of wiping the area with 70% ethanol after mopping up spillage in a cell culture laboratory?
To disinfect the work area
What are the two main categories of cell culture contaminants?
Biological and chemical contaminants
Why is it important to follow good aseptic technique in cell culture laboratory?
To reduce the frequency and seriousness of biological contamination
What is a common problem in cell cultures, with serious consequences?
Cross-contamination with HeLa and other fast growing cell lines
What type of filter was used to obtain the images of the cell cultures?
450 ± 30 nm bandpass filter
Why should antibiotics not be used routinely in cell culture?
All of the above
What is the recommended practice to avoid cross-contamination of cell lines?
Obtaining cell lines from reputable cell banks and periodically checking the characteristics of the cell lines
What analysis can confirm the presence or absence of cross-contamination in cell cultures?
DNA fingerprinting, karyotype analysis, and isotype analysis
When should antibiotics be used in cell culture?
As a last resort and only for short term applications
What is the primary goal of a biosafety program in a cell culture laboratory?
To reduce or eliminate exposure of laboratory workers and the environment to potentially harmful biological agents
What is a common hazard associated with handling cells and tissues in a laboratory?
Accidental punctures with syringe needles
What document provides regulations and recommendations for biosafety in the United States?
Biosafety in Microbiological and Biomedical Laboratories
What is the most important element of safety in a cell culture laboratory?
Strict adherence to standard microbiological practices and techniques
What is a potential route of exposure to infectious agents in a cell culture laboratory?
All of the above
What type of substances are commonly handled in a cell culture laboratory?
Toxic, corrosive, or mutagenic solvents and reagents
Test your knowledge on the specific hazards and safety risks associated with handling human or animal cells and tissues in a cell culture laboratory. Learn about the common hazards and how to prevent accidents.
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