Cell Biophysiology Overview
8 Questions
0 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to Lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What type of proteins do free polyribosomes primarily synthesize?

  • Proteins for lysosomal storage
  • Proteins secreted from the cell
  • Cytosolic and cytoskeletal proteins (correct)
  • Proteins for cell membrane incorporation
  • What is targeted for degradation by proteasomes when misfolded?

  • Ubiquitin-conjugated proteins (correct)
  • Cytoskeletal proteins
  • Ribosomes
  • Proteins synthesized by ribosomes
  • Which mechanism describes the elongation and turnover of actin filaments?

  • Stabilization at their ends
  • Dynamic equilibrium of microtubules
  • Treadmilling of actin filaments (correct)
  • Segregation of proteins in the ER
  • What is the primary function of the unfolded protein response (UPR)?

    <p>Manage misfolded proteins (B)</p> Signup and view all the answers

    What determines the fate of newly synthesized proteins?

    <p>Their amino acid sequence (A)</p> Signup and view all the answers

    How do proteins synthesized in the rough endoplasmic reticulum differ from those made by free ribosomes?

    <p>They are intended for incorporation into membranes (A)</p> Signup and view all the answers

    Which cytoskeletal component is primarily involved in cellular movement?

    <p>Actin filaments (B)</p> Signup and view all the answers

    What role do cytoskeletal dynamics play in the cell?

    <p>They influence intracellular trafficking and organelle positioning (D)</p> Signup and view all the answers

    Flashcards

    Protein Synthesis

    The process by which cells create proteins. It begins with DNA transcription and involves the formation of messenger RNA (mRNA), which carries genetic information from DNA to ribosomes, where protein synthesis takes place.

    Translation

    The process by which ribosomes attach to mRNA and synthesize proteins. Free ribosomes produce proteins that remain in the cytosol, while ribosomes bound to the endoplasmic reticulum (ER) produce proteins that are destined for the ER lumen, secretion, or integration into membranes.

    Signal Hypothesis

    A hypothesis that explains how proteins are targeted to specific locations within a cell. The signal hypothesis proposes that proteins destined for the ER, secretion, or membranes contain a signal sequence, a short stretch of amino acids that directs the ribosome to bind to the ER membrane.

    Unfolded Protein Response (UPR)

    A quality control mechanism within cells that deals with misfolded proteins. Essentially, it ensures the correct folding of proteins. If a protein misfolds, the unfolded protein response is activated, which can lead to the degradation of the misfolded protein or even programmed cell death (apoptosis).

    Signup and view all the flashcards

    Proteasome

    Large protein complexes that are responsible for the degradation of proteins tagged with ubiquitin. Ubiquitin is like a 'death tag' that marks proteins for destruction. The proteasome then breaks down the tagged protein into smaller peptides.

    Signup and view all the flashcards

    Cytoskeleton

    A network of protein filaments that provides structural support, helps with intracellular movement, and defines cell shape. It is composed of three primary types of filaments: microtubules, microfilaments, and intermediate filaments.

    Signup and view all the flashcards

    Treadmilling of Actin Filaments

    A process that involves the dynamic assembly and disassembly of actin filaments. Actin filaments are like tiny threads that can grow or shrink, changing the shape and movement of cells.

    Signup and view all the flashcards

    Dynamic Instability of Microtubules

    A process that describes the instability of microtubules, which can rapidly grow or shrink depending on the cellular needs. This dynamic behaviour is essential for cell division, transport of organelles, and cell movement.

    Signup and view all the flashcards

    Study Notes

    Cell Biophysiology - Learning Objectives

    • Describe protein synthesis
    • State the fate of proteins synthesized in the rough endoplasmic reticulum (rER) and those synthesized by free ribosomes
    • Explain the "signal hypothesis" in protein synthesis
    • List factors determining the fate of newly synthesized proteins
    • Explain the function of the unfolded protein response (UPR) and its importance to the cell
    • Compare protein degradation mechanisms using proteasomes or lysosomes
    • Explain the cytoskeleton's role in cell shape, organelle positioning, intracellular trafficking, and movement
    • Compare the characteristics and functions of microfilaments, microtubules, and intermediate filaments
    • Explain treadmilling of actin filaments and dynamic instability of microtubules
    • Explain how cytoskeletal dynamics control cellular movement
    • Identify and correlate organelles with their functions

    Chromatin structure

    • Chromatin is DNA bound to histones and other proteins
    • Chromatin exists in two forms:
      • Heterochromatin: Condensed chromatin, transcriptionally inactive, appears intensely stained
      • Euchromatin: Dispersed, unpacked chromatin, often actively transcribed, appears lightly stained
    • Nucleolus: Site of ribosomal RNA (rRNA) transcription and ribosome production; appears intensely stained

    Constitutive and Facultative Heterochromatin

    • Constitutive heterochromatin: Repetitive, stays condensed throughout the cell cycle
    • Facultative heterochromatin: Non-repetitive, can lose condensed state and become transcriptionally active

    Centrosome and Centriole

    • Centrosome: Microtubule organizing center in animal cells, consisting of:
      • Centrioles: Orthogonal pairs of centrioles
      • Pericentriolar material (PCM): Amorphous mass of proteins; responsible for microtubule anchoring. Cylindrical structure, ~200 nm in diameter, composed of nine triplets of microtubules

    Polyribosomes (Polysomes)

    • Multiple ribosomes bound to a single mRNA molecule
    • Simultaneously translate mRNA into many protein copies

    Polyribosomes: Free and ER-Bound

    • Free polyribosomes synthesize cytosolic and cytoskeletal proteins and proteins for import into the nucleus, mitochondria, and peroxisomes
    • ER-bound polyribosomes synthesize proteins for incorporation into membranes, storage in lysosomes, and secretion from the cell

    Protein Synthesis - Signal Hypothesis

    • Signal hypothesis describes protein translocation into the endoplasmic reticulum (ER)
    • Signal peptide: Sequence at the N-terminus of a protein targeted for the ER
    • Signal recognition particle (SRP): Binds to the signal peptide
    • SRP-receptor binds to the SRP, halting translation
    • Ribosome-translocator complex: Ribosome docks on the ER membrane
    • Signal peptidase: Removes the signal peptide
    • Chaperones: Assist with proper polypeptide folding in the ER cisterna

    ER-Associated Degradation (ERAD)

    • ERAD pathway degrades misfolded or unfolded proteins
    • Ubiquitin proteins are conjugated to misfolded proteins
    • Degraded by proteasomes

    Unfolded Protein Response (UPR)

    • UPR triggers when unfolded proteins accumulate in the ER
    • Adjusts the number of proteins that need to be folded
    • Increases endoplasmic reticulum's protein folding ability
    • Removes proteins that take longer to fold

    Ubiquitin-Proteasome Pathway

    • Ubiquitin-protein ligation: Ubiquitin attached to a protein via enzymes
    • Recognition of the ubiquitin-conjugated protein by the 26S proteasome
    • Degradation of the ubiquitin-conjugated protein by the 26S proteasome
    • Release and recycling of ubiquitin
    • Disassembly of the polyubiquitin chain

    Self-Study Slides

    • Organelle identification and correlating with function

    Studying That Suits You

    Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

    Quiz Team

    Related Documents

    Cell Biophysiology PDF

    Description

    This quiz covers essential learning objectives in cell biophysiology, focusing on protein synthesis, the fate of proteins, and the function of the cytoskeleton. Explore the roles of organelles, chromatin structure, and cellular movement mechanisms. Test your understanding of key concepts related to cell structure and function.

    More Like This

    Science Quiz Protein Synthesis
    15 questions

    Science Quiz Protein Synthesis

    SolicitousPelican7010 avatar
    SolicitousPelican7010
    Protein Synthesis Overview
    11 questions
    Use Quizgecko on...
    Browser
    Browser