Cell Biology: Organelles Overview
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Cell Biology: Organelles Overview

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Questions and Answers

What type of molecules can cross the plasma membrane without any assistance?

  • Hydrophilic proteins
  • Small, non-polar molecules (correct)
  • Large polar molecules
  • Charged ions
  • What property of the plasma membrane prevents most water-soluble molecules from passing through?

  • The hydrophobic lipid bilayer (correct)
  • The presence of cholesterol
  • Ion channels in the membrane
  • The membrane's selective permeability
  • Why are charged ions unable to cross the plasma membrane freely?

  • They require specific carriers for transport
  • They are too large to fit through membrane pores
  • The hydrophobic fatty acids repel them
  • Their charge creates strong attraction to water (correct)
  • Which of the following is a mechanism that allows substances to cross the plasma membrane?

    <p>Multiple transport mechanisms including diffusion and facilitated diffusion</p> Signup and view all the answers

    What substances typically need assistance to pass through the plasma membrane?

    <p>Larger polar molecules and charged ions</p> Signup and view all the answers

    What are the main functions of peroxisomes in a cell?

    <p>Breaking down fatty acids and removing toxic substances</p> Signup and view all the answers

    Which statement correctly describes the structure of a chloroplast?

    <p>It has an outer membrane, an inner membrane, thylakoids, and stroma.</p> Signup and view all the answers

    What types of molecular structures compose the cytoskeleton?

    <p>Microtubules, microfilaments, and intermediate filaments</p> Signup and view all the answers

    What is the primary role of centrioles in animal cells?

    <p>Forming spindle fibers during cell division</p> Signup and view all the answers

    What is the function of microtubules in cellular processes?

    <p>They interact with motor proteins to facilitate motility and structural organization.</p> Signup and view all the answers

    What is a defining characteristic of passive transport?

    <p>It involves movement from high to low concentration.</p> Signup and view all the answers

    Which of the following molecules transport through facilitated diffusion?

    <p>Glucose and amino acids</p> Signup and view all the answers

    What role do transport proteins play in facilitated diffusion?

    <p>They shield molecules from the hydrophobic core of the membrane.</p> Signup and view all the answers

    During passive transport, when does the transport process cease?

    <p>When equilibrium is reached.</p> Signup and view all the answers

    Which type of transport movement uses energy?

    <p>Active transport</p> Signup and view all the answers

    What role do carbohydrates play in the plasma membrane?

    <p>Form a carbohydrate layer for cellular protection</p> Signup and view all the answers

    What is the primary function of transmembrane proteins?

    <p>Facilitate transport of substances across the membrane</p> Signup and view all the answers

    Which statement accurately describes phospholipids?

    <p>They create a stable barrier between aqueous compartments.</p> Signup and view all the answers

    What distinguishes multi-pass transmembrane proteins from single-pass proteins?

    <p>Multi-pass proteins cross the membrane multiple times.</p> Signup and view all the answers

    Where are oligosaccharides located in the plasma membrane?

    <p>On the outer surface, forming a sugar coating</p> Signup and view all the answers

    Which of the following accurately describes the role of cholesterol in the plasma membrane?

    <p>Offers stability to the membrane structure</p> Signup and view all the answers

    In which way do peripheral proteins differ from transmembrane proteins?

    <p>Peripheral proteins are associated with the inner or outer surface of the membrane.</p> Signup and view all the answers

    What is one function of the carbohydrate layer on the cell surface?

    <p>To prevent mechanical and chemical damage</p> Signup and view all the answers

    Which of the following describes the arrangement of phospholipids in the plasma membrane?

    <p>Hydrophobic tails facing each other with hydrophilic heads outward</p> Signup and view all the answers

    Which process must occur for cells to efficiently exchange molecules with their environment?

    <p>Transport of substances across the plasma membrane</p> Signup and view all the answers

    What distinguishes transporters from channels in moving molecules across membranes?

    <p>Transporters bind to molecules, while channels allow passage through a pore.</p> Signup and view all the answers

    What is the primary function of aquaporins in a cell membrane?

    <p>To facilitate the diffusion of water.</p> Signup and view all the answers

    In a hypertonic solution, which of the following occurs in a cell?

    <p>Water leaves the cell, causing it to shrink.</p> Signup and view all the answers

    What happens during osmosis?

    <p>Water moves from an area of high concentration to an area of low concentration.</p> Signup and view all the answers

    What is a characteristic of active transport?

    <p>It requires energy in the form of ATP.</p> Signup and view all the answers

    Which of the following best describes endocytosis?

    <p>The engulfing of substances by the cell membrane to bring them into the cell.</p> Signup and view all the answers

    What occurs in a hypotonic solution?

    <p>Water enters the cells, causing them to swell and possibly burst.</p> Signup and view all the answers

    What type of transport involves many substances crossing a membrane together?

    <p>Bulk transport.</p> Signup and view all the answers

    Study Notes

    Mitochondria

    • The intermembrane space is the narrow region between the inner and outer mitochondrial membranes.
    • The mitochondrial matrix is the space enclosed by the inner mitochondrial membrane.
    • Some metabolic steps of cellular respiration are catalyzed within the mitochondrial matrix.

    Chloroplasts

    • Chloroplasts are found in plants and algae.
    • They are the sites of photosynthesis.
    • Chloroplasts contain chlorophyll, which is a green pigment.
    • They also contain enzymes and other molecules that function in photosynthesis.
    • Chloroplasts are found in leaves and green organs of plants/algae.
    • The internal structure of a chloroplast includes an outer membrane, an inner membrane, thylakoids, and stroma.
    • Thylakoids are membranous sacs, stacked to form a granum.
    • Stroma is the internal fluid within a chloroplast.

    Peroxisomes

    • Peroxisomes are specialized metabolic compartments bounded by a single membrane.
    • They participate in oxidation reactions.
    • Peroxisomes remove cell toxic substances, particularly excess of toxic oxygen radicals.
    • They break down fatty acids, a process important for fat metabolism.

    Cytoskeleton

    • The cytoskeleton is a network of fibers extending throughout the cytoplasm.
    • It organizes the cell’s structures and activities and anchors many organelles.
    • The cytoskeleton is composed of three types of molecular structures: microtubules, microfilaments, and intermediate filaments.
    • The cytoskeleton helps to support the cell and maintain its shape.
    • It interacts with motor proteins to produce cell motility.
    • Vesicles can travel along tracks provided by the cytoskeleton.

    Centrioles

    • Centrioles are paired barrel-shaped organelles located in the cytoplasm of animal cells near the nuclear envelope.
    • Two centrioles together form the centrosome.

    Centrosome

    • In animal cells, all microtubules (spindle fibers) in the cell grow out from a centrosome located near the nucleus.
    • The centrosome is involved in the development of microtubules during cell division.
    • Microtubules control the movement of membrane, allowing for seamless reassembly after separation caused by objects like needles.

    Plasma Membrane

    • The plasma membrane is the outer boundary of the cell, controlling what enters and exits.
    • It consists of three main components: lipids, proteins, and carbohydrates.

    Lipids

    • Phospholipids are amphipathic molecules, with hydrophobic tails pointing inward and hydrophilic heads pointing outward, forming a phospholipid bilayer.
    • This bilayer forms a stable barrier between two aqueous compartments.
    • Cholesterol is found alongside phospholipids in the core of the membrane (only in animal cells) and provides stability to the membrane.

    Proteins

    • The membrane contains various types of embedded proteins that carry out most membrane functions.
    • They act as transporters, anchors, receptors, and enzymes.
    • Transmembrane proteins can cross the membrane once (single-pass) or several times (multi-pass) using α-helixes containing hydrophobic amino acids.
    • Peripheral proteins are associated with the membrane either inside or outside the cell, interacting with phospholipids or other proteins.

    Carbohydrates

    • Sugar groups (oligosaccharides) are present only on the outer surface of the plasma membrane, facing the outside of the cell.
    • They are linked to proteins (glycoproteins) or lipids (glycolipids), forming a carbohydrate layer.
    • This carbohydrate layer protects the cell, absorbs water to create a slimy surface for motility, and acts as a distinctive cellular marker.

    Carbohydrate Functions

    • The carbohydrate layer helps protect the cell from mechanical and chemical damage.
    • It absorbs water, giving the cell a slimy surface and aiding in motility, especially for white blood cells squeezing through narrow spaces.
    • The carbohydrate layer forms distinctive cellular markers, acting as "molecular ID badges" for cell recognition.

    Cellular Recognition Examples

    • Carbohydrate markers participate in the recognition of egg by sperm, white blood cells recognizing cancer cells, white blood cells recognizing pathogens (bacteria, viruses, etc.), and early stages of a bacterial infection by white blood cells.

    Membrane Transport

    • The plasma membrane acts as a barrier for molecules going in and out of the cell.
    • Cells need to exchange molecules with their environment to survive.
    • Important molecules transported in and out of the cell include nutrients (food, sugars, amino acids, salts, ions), wastes, ions, carbon dioxide, and water.
    • The membrane is impermeable to most substances due to the hydrophobic interior of the lipid bilayer, which hinders the passage of hydrophilic molecules.

    Transport Without Help

    • Small, non-polar molecules like gases (oxygen, carbon dioxide, nitrogen) and steroid hormones can readily dissolve in phospholipids and diffuse across the membrane.

    Transport With Help

    • Larger molecules, polar molecules (water, glucose, amino acids, proteins), and charged ions cannot pass through the membrane without assistance.
    • The membrane uses several mechanisms to transport these substances, allowing for cellular regulation of what goes in and out.

    Diffusion

    • Diffusion is the movement of a substance from a region where it is more concentrated to one where it is less concentrated.

    Passive Transport

    • Passive transport is the movement of materials across the membrane that requires no cellular energy (no ATP).
    • It occurs down a concentration gradient, from high concentration to low concentration.
    • Simple diffusion and facilitated diffusion are the types of passive transport.

    Simple Diffusion

    • Materials diffuse directly through the phospholipids, moving from high to low concentration.
    • This applies to gases (oxygen, carbon dioxide, nitrogen) and small lipids (steroid hormones).
    • Diffusion continues until equilibrium is reached, resulting in equal concentrations on both sides of the membrane.

    Facilitated Diffusion

    • This type of transport requires the assistance of specialized proteins in the membrane.
    • Hydrophilic, large, polar, or charged molecules that cannot diffuse through the phospholipids rely on transport proteins.
    • Transport proteins shield these molecules from the hydrophobic core of the membrane, providing a route for them to cross.
    • Transport occurs down a concentration gradient, from higher to lower concentration, stopping when equilibrium is reached.
    • Facilitated diffusion transports water, large molecules (glucose, amino acids), and charged molecules (ions: Ca2+, Na+, K+, Cl-).

    Membrane Transport Proteins

    • Two main types of membrane transport proteins exist: transporters and channels.

    Transporters

    • Transporters bind to the molecule to be transferred and then move it to the other side.
    • They are specific and selective, like the glucose transporter that only moves glucose.
    • Carrier proteins change shape to move a target molecule from one side of the membrane to the other.
    • They move a wide variety of water-soluble molecules across membranes but at a slower rate than channels.

    Channels

    • Channels function like a pore with a central space allowing molecules to pass.
    • They are specific, discriminating based on size and electric charge.
    • For instance, Na+ channels only transport Na+.
    • Water transport through facilitated diffusion uses a special channel called aquaporin.

    Osmosis

    • Osmosis is the movement of water in and out of the cell depending on the water concentration on both sides of the membrane.
    • Water moves from a higher concentration of water to a lower concentration of water.

    Isotonic Solution

    • In an isotonic solution, the concentration of solutes and water outside the cell is the same as inside the cell.
    • There is no net movement of water.
    • The volume of the cell does not change.

    Hypertonic Solution

    • In a hypertonic solution, there are more solutes and less water outside the cell compared to inside the cell.
    • Osmotic pressure is higher inside the cell than outside, so water diffuses out.
    • The cell shrinks.

    Hypotonic Solution

    • In a hypotonic solution, there are fewer solutes and more water outside the cell compared to inside the cell.
    • Water diffuses into the cell, moving from a higher water concentration to a lower water concentration.
    • The cell swells and can burst.

    Active Transport

    • Active transport is a type of transport across the membrane where molecules move against the concentration gradient, from a region of low concentration to high concentration.
    • This process requires energy (ATP) to pump substances across the membrane.
    • Active transport requires the assistance of membrane proteins, where embedded proteins change shape to open or close passages across the membrane.

    Bulk Transport

    • Bulk transport involves the movement of many substances together "in bulk" through the membrane via bound vesicles.
    • This is how many larger particles enter the cell.

    Endocytosis

    • Endocytosis is a process of capturing a substance or particle from outside the cell by engulfing it with the cell membrane and bringing it into the cell.
    • The cell captures material "in bulk" from the outside.
    • Endocytosis has three types: phagocytosis, pinocytosis, and receptor-mediated endocytosis.

    Phagocytosis

    • Phagocytosis is the process of engulfing large solid particles, such as bacteria or cell debris.
    • The cell membrane surrounds the particle, forming a phagosome that fuses with a lysosome for digestion.

    Pinocytosis

    • Pinocytosis is the process of engulfing small droplets of extracellular fluid, containing dissolved substances.
    • The cell membrane forms tiny pockets that pinch off as vesicles containing the fluid.

    Receptor-Mediated Endocytosis

    • Receptor-mediated endocytosis is a highly specific process where the cell takes in specific molecules that bind to receptors on the cell surface.
    • These receptors reside in coated pits, depressions in the membrane coated with a protein called clathrin.
    • When a specific molecule binds to the receptor, the coated pit forms a vesicle that carries the molecule inside the cell.

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