Lecture 31 – Gluconeogenesis and Pentose Phosphate Pathway

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Questions and Answers

What is the primary purpose of gluconeogenesis?

  • To convert pyruvate into glucose (correct)
  • To produce ATP
  • To generate NADPH
  • To break down glucose for energy

Which enzyme catalyzes the phosphorylation of glucose to glucose-6-phosphate in glycolysis?

  • Hexokinase (correct)
  • Pyruvate kinase
  • Phosphofructokinase
  • Glyceraldehyde-3-phosphate dehydrogenase

What is the nature of reactions with a large negative ΔG in metabolic pathways?

  • They are reversible.
  • They are independent of substrate concentration.
  • They are irreversible. (correct)
  • They require ATP.

Which compound can serve as a starting material for gluconeogenesis?

<p>Lactate (B)</p> Signup and view all the answers

What is the role of NADPH in the pentose phosphate pathway (PPP)?

<p>Biosynthesis and antioxidant defense (C)</p> Signup and view all the answers

What are the two main stages of the pentose phosphate pathway?

<p>Oxidative and non-oxidative (B)</p> Signup and view all the answers

Which enzyme catalyzes the conversion of glucose-6-phosphate to ribulose-5-phosphate?

<p>Glucose-6-phosphate dehydrogenase (A)</p> Signup and view all the answers

In the oxidative phase of the PPP, how many NADPH molecules are produced from one molecule of glucose-6-phosphate?

<p>2 (B)</p> Signup and view all the answers

Which enzyme catalyzes the conversion of glucose-6-phosphate to ribulose-5-phosphate?

<p>Glucose-6-phosphate dehydrogenase (A)</p> Signup and view all the answers

What is the outcome of the non-oxidative phase of the PPP?

<p>Production of ribose-5-phosphate and glycolytic intermediates (B)</p> Signup and view all the answers

What is the main regulatory step in the pentose phosphate pathway?

<p>Conversion of glucose-6-phosphate to ribulose-5-phosphate (B)</p> Signup and view all the answers

What is the primary function of ribose-5-phosphate produced in the PPP?

<p>DNA and RNA synthesis (B)</p> Signup and view all the answers

In which mode of the PPP is ribose-5-phosphate maximized?

<p>Mode 1 (A)</p> Signup and view all the answers

Which pathway produces ribose-5-phosphate needed for nucleotide synthesis?

<p>Pentose phosphate pathway (C)</p> Signup and view all the answers

What condition leads to the activation of glycolytic intermediates in the PPP?

<p>High ribose-5-phosphate demand (C)</p> Signup and view all the answers

Favism is associated with a deficiency in which enzyme?

<p>Glucose-6-phosphate dehydrogenase (A)</p> Signup and view all the answers

What is a consequence of glucose-6-phosphate dehydrogenase deficiency?

<p>Hemolytic anemia (B)</p> Signup and view all the answers

Which compound is NOT a substrate for gluconeogenesis?

<p>Acetyl-CoA (D)</p> Signup and view all the answers

What is the net reaction for the oxidative phase of the PPP?

<p>Glucose-6-P + 12 NADP+ + 7 H2O → 6 CO2 + 12 NADPH + 12 H+ + Pi (A)</p> Signup and view all the answers

What is the role of NADP+ in the oxidative phase of the PPP?

<p>It functions as an electron acceptor. (B)</p> Signup and view all the answers

In which cellular location does gluconeogenesis primarily occur?

<p>Cytosol (A)</p> Signup and view all the answers

Which of the following best describes a futile cycle?

<p>A cycle that consumes energy without producing useful work (B)</p> Signup and view all the answers

What is the end product of the oxidative phase of the PPP?

<p>NADPH (B)</p> Signup and view all the answers

What is the significance of the cyclic mode of the PPP?

<p>It maximizes ATP production. (A)</p> Signup and view all the answers

In which scenario would a cell primarily utilize Mode 3 of the PPP?

<p>Adipose tissue or red blood cells needing NADPH (C)</p> Signup and view all the answers

Which of the following is an example of an anaplerotic reaction?

<p>Conversion of pyruvate to oxaloacetate (B)</p> Signup and view all the answers

What is the main energy cost associated with gluconeogenesis?

<p>Consumption of ATP and GTP (A)</p> Signup and view all the answers

Which of the following processes is directly inhibited by malonate?

<p>Citric acid cycle (B)</p> Signup and view all the answers

What happens to the levels of NADPH during oxidative stress?

<p>They remain unchanged. (C)</p> Signup and view all the answers

What is the primary function of the non-oxidative phase of the PPP?

<p>Production of intermediates for nucleotide synthesis (B)</p> Signup and view all the answers

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