Questions and Answers
What is the objective of the course BT5005: Genetic Engineering and rDNA Technology?
Genetic engineering and recombinant DNA technology are the same concepts.
False
Name one type of DNA modifying enzyme used in gene cloning.
Restriction enzymes
_______ vectors are used in gene cloning and can include plasmid vectors, phage vectors, cosmids, and yeast vectors.
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What are the steps involved in gene cloning?
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Name the molecular tools commonly used for gene cloning.
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Is gene therapy considered one of the applications of genetic engineering?
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The process of inserting a foreign DNA fragment into a vector typically involves linkers, adaptors, and ________ tailing.
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Match the following blotting techniques with their respective types:
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Study Notes
BT5005: Genetic Engineering and rDNA Technology
- The course aims to impart fundamental knowledge about theoretical aspects and applications of rDNA technology and genetic engineering for human benefits.
Objectives
- Acquire adequate knowledge and necessary skills related to different techniques involved in genetic engineering.
- Understand the objective of gene cloning, library preparation, and screening of positive clones from the library.
- Learn about advanced techniques of Recombinant DNA Technology and genetic engineering.
Course Outcomes
- CO1: Understand the players of gene cloning, including DNA modifying enzymes, cloning vectors, and expression vectors.
- CO2: Learn the steps of gene cloning, library preparation, and screening of positive clones from the library.
- CO3: Understand advanced techniques of Recombinant DNA Technology, including blotting, DNA sequencing, PCR, and mutagenesis.
- CO4: Acquire knowledge about recombinant proteins, gene therapy, and advanced techniques of genetic engineering.
Course Structure
- Unit I: Introduction to Gene Cloning (12 hours)
- Molecular tools for gene cloning, including enzymes, nucleases, and ligases.
- Cloning vectors and their desirable properties.
- Expression vectors, including pET, pMAl, and pGEX.
- Unit II: Strategies of Genetic Engineering (12 hours)
- Isolation of DNA to be cloned, including genomic and plasmid DNA.
- Construction of genomic and cDNA libraries.
- Methods of gene transfer, including transformation and growth of cells.
- Selection of clones and recombinant selection methods.
- Unit III: Advanced Techniques of rDNA Technology (12 hours)
- Blotting techniques, including Southern, Northern, and Western blotting.
- DNA sequencing, PCR, and real-time PCR.
- Mutagenesis, protein engineering, and protein-DNA interactions.
- Unit IV: Applications of Genetic Engineering (12 hours)
- Recombinant products, including therapeutic proteins and vaccines.
- Applications of gene cloning in agriculture, medicine, and environmental and forensic science.
- Gene therapy.
Practical
- Isolation of genomic DNA and quantification.
- PCR amplification and analysis by agarose gel electrophoresis.
- Isolation of total RNA and quantification.
- Preparation of plasmid, pET-28a, and analysis.
- Restriction digestion analysis of a given plasmid.
- Preparation of competent cells and transformation in E. coli.
Reference Books
- Biotechnology: The Biological Principles by M.D. Trevan et al.
- Biotechnology by Smith, Cambridge Press.
- Advanced Molecular Biology by Twyman R.M.
- Microbiology by Atlas R.M.
- Microbiology- Prescott L.M.
- Microbial Genetics by Freifelder D.
- Principles of Gene Manipulation by Old and Primrose.
- Principles of Gene Manipulation and Genome by Primrose and Twyman.
Programme Outcomes
- PO1: Knowledge of microbiological sciences and their application in industry and hospitals.
- PO2: Core Competence in microbiology, including the ability to find remedies for diseases.
- PO3: Breadth of knowledge in microbiology, including its impact on human health and the environment.
- PO4: Preparation for research projects and industrial applications.
- PO5: Professionalism in microbiology, including career opportunities.
- PO6: Evaluation of academic performance based on continuous internal evaluation and semester-end examinations.
BT5005: Genetic Engineering and rDNA Technology
- The course aims to impart fundamental knowledge about theoretical aspects and applications of rDNA technology and genetic engineering for human benefits.
Objectives
- Acquire adequate knowledge and necessary skills related to different techniques involved in genetic engineering.
- Understand the objective of gene cloning, library preparation, and screening of positive clones from the library.
- Learn about advanced techniques of Recombinant DNA Technology and genetic engineering.
Course Outcomes
- CO1: Understand the players of gene cloning, including DNA modifying enzymes, cloning vectors, and expression vectors.
- CO2: Learn the steps of gene cloning, library preparation, and screening of positive clones from the library.
- CO3: Understand advanced techniques of Recombinant DNA Technology, including blotting, DNA sequencing, PCR, and mutagenesis.
- CO4: Acquire knowledge about recombinant proteins, gene therapy, and advanced techniques of genetic engineering.
Course Structure
- Unit I: Introduction to Gene Cloning (12 hours)
- Molecular tools for gene cloning, including enzymes, nucleases, and ligases.
- Cloning vectors and their desirable properties.
- Expression vectors, including pET, pMAl, and pGEX.
- Unit II: Strategies of Genetic Engineering (12 hours)
- Isolation of DNA to be cloned, including genomic and plasmid DNA.
- Construction of genomic and cDNA libraries.
- Methods of gene transfer, including transformation and growth of cells.
- Selection of clones and recombinant selection methods.
- Unit III: Advanced Techniques of rDNA Technology (12 hours)
- Blotting techniques, including Southern, Northern, and Western blotting.
- DNA sequencing, PCR, and real-time PCR.
- Mutagenesis, protein engineering, and protein-DNA interactions.
- Unit IV: Applications of Genetic Engineering (12 hours)
- Recombinant products, including therapeutic proteins and vaccines.
- Applications of gene cloning in agriculture, medicine, and environmental and forensic science.
- Gene therapy.
Practical
- Isolation of genomic DNA and quantification.
- PCR amplification and analysis by agarose gel electrophoresis.
- Isolation of total RNA and quantification.
- Preparation of plasmid, pET-28a, and analysis.
- Restriction digestion analysis of a given plasmid.
- Preparation of competent cells and transformation in E. coli.
Reference Books
- Biotechnology: The Biological Principles by M.D. Trevan et al.
- Biotechnology by Smith, Cambridge Press.
- Advanced Molecular Biology by Twyman R.M.
- Microbiology by Atlas R.M.
- Microbiology- Prescott L.M.
- Microbial Genetics by Freifelder D.
- Principles of Gene Manipulation by Old and Primrose.
- Principles of Gene Manipulation and Genome by Primrose and Twyman.
Programme Outcomes
- PO1: Knowledge of microbiological sciences and their application in industry and hospitals.
- PO2: Core Competence in microbiology, including the ability to find remedies for diseases.
- PO3: Breadth of knowledge in microbiology, including its impact on human health and the environment.
- PO4: Preparation for research projects and industrial applications.
- PO5: Professionalism in microbiology, including career opportunities.
- PO6: Evaluation of academic performance based on continuous internal evaluation and semester-end examinations.
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Description
This quiz covers the concepts of genetic engineering and rDNA technology for B.Sc. Microbiology students in their 5th semester.
