18 Questions
Which specimen is the specimen of choice for Brucellosis diagnosis?
Blood
What is one of the most common sources of laboratory-acquired Brucellosis?
Inhalation of infectious aerosols
Which method can be used for direct detection of Brucellae from clinical specimens?
Real-time PCR
How can neonatal Brucellosis be acquired?
Via ingestion of contaminated breast milk
What are the main prevention and control measures for Brucellosis?
Proper hand hygiene and protective gear for veterinarians
Which specimen is rarely used to isolate Brucella organisms?
Urinary samples
What is the most definitive method for the diagnosis of brucellosis?
Blood culture
Which method is NOT useful for demonstrating Brucella organism in clinical specimens?
Gram staining
How long should subcultures be made on solid media before declaring a culture as negative?
8 weeks
What is a recommended prevention measure for brucellosis?
Pasteurization of milk
Which strain is used for vaccinating cattle against brucellosis?
Brucella abortus strain 16
What is the optimal temperature for incubating blood cultures for brucellosis diagnosis?
37 degrees C
What is the effect of adding bacitracin, polymyxin, and cycloheximide to certain media used for culturing Brucellae?
They make the media selective
How do colonies of Brucellae typically appear on solid media after several days of incubation?
Small, moist, translucent, and glistening
Which of the following statements about Brucellae's resistance is TRUE?
Survives for months in refrigerated milk
Which of the following is a characteristic feature of Brucellae based on biochemical reactions?
Urease negative
How are Brucellae classified into different species?
By lysis from specific Bacteriophage
What is a key feature of Br.melitensis in terms of biotypes?
3 biotypes
Learn about the various methods used for the culture and isolation of Brucella organisms in clinical specimens. Understand why Gram staining is not useful for demonstrating Brucella due to their small size and intracellular location. Explore techniques such as Castaneda’s method, automated methods like bactec, and the lysis centrifugation system.
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