Brucella Diagnostic Methods
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Questions and Answers

Which specimen is the specimen of choice for Brucellosis diagnosis?

  • Sputum
  • Urine
  • Blood (correct)
  • Liver
  • What is one of the most common sources of laboratory-acquired Brucellosis?

  • Oral ingestion
  • Inhalation of infectious aerosols (correct)
  • Ingestion of contaminated dairy products
  • Skin contact with infected animals
  • Which method can be used for direct detection of Brucellae from clinical specimens?

  • Blood smear microscopy
  • Real-time PCR (correct)
  • Culture
  • Hypersensitivity tests
  • How can neonatal Brucellosis be acquired?

    <p>Via ingestion of contaminated breast milk</p> Signup and view all the answers

    What are the main prevention and control measures for Brucellosis?

    <p>Proper hand hygiene and protective gear for veterinarians</p> Signup and view all the answers

    Which specimen is rarely used to isolate Brucella organisms?

    <p>Urinary samples</p> Signup and view all the answers

    What is the most definitive method for the diagnosis of brucellosis?

    <p>Blood culture</p> Signup and view all the answers

    Which method is NOT useful for demonstrating Brucella organism in clinical specimens?

    <p>Gram staining</p> Signup and view all the answers

    How long should subcultures be made on solid media before declaring a culture as negative?

    <p>8 weeks</p> Signup and view all the answers

    What is a recommended prevention measure for brucellosis?

    <p>Pasteurization of milk</p> Signup and view all the answers

    Which strain is used for vaccinating cattle against brucellosis?

    <p>Brucella abortus strain 16</p> Signup and view all the answers

    What is the optimal temperature for incubating blood cultures for brucellosis diagnosis?

    <p>37 degrees C</p> Signup and view all the answers

    What is the effect of adding bacitracin, polymyxin, and cycloheximide to certain media used for culturing Brucellae?

    <p>They make the media selective</p> Signup and view all the answers

    How do colonies of Brucellae typically appear on solid media after several days of incubation?

    <p>Small, moist, translucent, and glistening</p> Signup and view all the answers

    Which of the following statements about Brucellae's resistance is TRUE?

    <p>Survives for months in refrigerated milk</p> Signup and view all the answers

    Which of the following is a characteristic feature of Brucellae based on biochemical reactions?

    <p>Urease negative</p> Signup and view all the answers

    How are Brucellae classified into different species?

    <p>By lysis from specific Bacteriophage</p> Signup and view all the answers

    What is a key feature of Br.melitensis in terms of biotypes?

    <p>3 biotypes</p> Signup and view all the answers

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