Brucella Diagnostic Methods
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Questions and Answers

Which specimen is the specimen of choice for Brucellosis diagnosis?

  • Sputum
  • Urine
  • Blood (correct)
  • Liver

What is one of the most common sources of laboratory-acquired Brucellosis?

  • Oral ingestion
  • Inhalation of infectious aerosols (correct)
  • Ingestion of contaminated dairy products
  • Skin contact with infected animals

Which method can be used for direct detection of Brucellae from clinical specimens?

  • Blood smear microscopy
  • Real-time PCR (correct)
  • Culture
  • Hypersensitivity tests

How can neonatal Brucellosis be acquired?

<p>Via ingestion of contaminated breast milk (C)</p> Signup and view all the answers

What are the main prevention and control measures for Brucellosis?

<p>Proper hand hygiene and protective gear for veterinarians (C)</p> Signup and view all the answers

Which specimen is rarely used to isolate Brucella organisms?

<p>Urinary samples (B)</p> Signup and view all the answers

What is the most definitive method for the diagnosis of brucellosis?

<p>Blood culture (B)</p> Signup and view all the answers

Which method is NOT useful for demonstrating Brucella organism in clinical specimens?

<p>Gram staining (B)</p> Signup and view all the answers

How long should subcultures be made on solid media before declaring a culture as negative?

<p>8 weeks (D)</p> Signup and view all the answers

What is a recommended prevention measure for brucellosis?

<p>Pasteurization of milk (B)</p> Signup and view all the answers

Which strain is used for vaccinating cattle against brucellosis?

<p>Brucella abortus strain 16 (A)</p> Signup and view all the answers

What is the optimal temperature for incubating blood cultures for brucellosis diagnosis?

<p>37 degrees C (C)</p> Signup and view all the answers

What is the effect of adding bacitracin, polymyxin, and cycloheximide to certain media used for culturing Brucellae?

<p>They make the media selective (B)</p> Signup and view all the answers

How do colonies of Brucellae typically appear on solid media after several days of incubation?

<p>Small, moist, translucent, and glistening (D)</p> Signup and view all the answers

Which of the following statements about Brucellae's resistance is TRUE?

<p>Survives for months in refrigerated milk (D)</p> Signup and view all the answers

Which of the following is a characteristic feature of Brucellae based on biochemical reactions?

<p>Urease negative (A)</p> Signup and view all the answers

How are Brucellae classified into different species?

<p>By lysis from specific Bacteriophage (C)</p> Signup and view all the answers

What is a key feature of Br.melitensis in terms of biotypes?

<p>3 biotypes (B)</p> Signup and view all the answers

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