Podcast
Questions and Answers
What is the central dogma of molecular biology?
What is the central dogma of molecular biology?
- RNA is transcribed from protein, and DNA is translated from RNA.
- DNA is replicated into RNA, which is then translated into protein.
- Protein is replicated into RNA, which is then translated into DNA.
- DNA is transcribed into RNA, which is then translated into protein. (correct)
What did Avery, MacLeod, and McCarty's experiment demonstrate?
What did Avery, MacLeod, and McCarty's experiment demonstrate?
- Lipids are the key components for transmitting heritable traits.
- RNA is the molecule responsible for bacterial transformation.
- Proteins are the primary carriers of genetic information.
- DNA is the molecule responsible for bacterial transformation. (correct)
What is the role of proteins in the universal features of life?
What is the role of proteins in the universal features of life?
- They replicate DNA.
- They store hereditary information.
- They transcribe DNA into RNA.
- They act as catalysts. (correct)
How does the structure of DNA provide a mechanism for heredity?
How does the structure of DNA provide a mechanism for heredity?
What is the significance of the experiment conducted by Griffith using Streptococcus pneumoniae?
What is the significance of the experiment conducted by Griffith using Streptococcus pneumoniae?
Which of the following is a characteristic exclusive to eukaryotic DNA?
Which of the following is a characteristic exclusive to eukaryotic DNA?
Which concept did the experiments conducted by Avery, MacLeod, and McCarty clarify about genetic material?
Which concept did the experiments conducted by Avery, MacLeod, and McCarty clarify about genetic material?
What is the key feature of DNA's double helix structure that allows it to be accurately replicated?
What is the key feature of DNA's double helix structure that allows it to be accurately replicated?
What insight did the work of Phoebus Levene provide regarding the structure of DNA?
What insight did the work of Phoebus Levene provide regarding the structure of DNA?
If a double-stranded DNA molecule globally contains 20% guanine, what percentage of adenine would it contain?
If a double-stranded DNA molecule globally contains 20% guanine, what percentage of adenine would it contain?
What is the primary significance of semi-conservative DNA replication?
What is the primary significance of semi-conservative DNA replication?
According to the universal features of life on Earth, how do cells replicate their hereditary information?
According to the universal features of life on Earth, how do cells replicate their hereditary information?
What structural feature is unique to RNA compared to DNA?
What structural feature is unique to RNA compared to DNA?
Which of the following represents the correct order of the central dogma of molecular biology?
Which of the following represents the correct order of the central dogma of molecular biology?
What would be the most likely structural consequence if a purine-purine base pairing occurred within a DNA double helix?
What would be the most likely structural consequence if a purine-purine base pairing occurred within a DNA double helix?
What is the relationship between a gene and a protein according to the universal features of life?
What is the relationship between a gene and a protein according to the universal features of life?
During DNA replication, what critical feature ensures the accurate transmission of hereditary information?
During DNA replication, what critical feature ensures the accurate transmission of hereditary information?
What role do proteins play in the context of the universal features of cellular life?
What role do proteins play in the context of the universal features of cellular life?
Which principle did Griffith's experiment with Streptococcus pneumoniae reveal about heredity?
Which principle did Griffith's experiment with Streptococcus pneumoniae reveal about heredity?
In the context of molecular biology, what is meant by 'templated polymerization' in DNA replication’?
In the context of molecular biology, what is meant by 'templated polymerization' in DNA replication’?
Which outcome accurately describes the result observed in Avery, Macleod, and McCarty’s experiments?
Which outcome accurately describes the result observed in Avery, Macleod, and McCarty’s experiments?
What is the significance of Chargaff's rules in the context of DNA structure?
What is the significance of Chargaff's rules in the context of DNA structure?
What is the critical role of complementary base pairing in the double helix structure of DNA?
What is the critical role of complementary base pairing in the double helix structure of DNA?
Why is DNA replication considered a semi-conservative process?
Why is DNA replication considered a semi-conservative process?
If a cell cannot perform transcription, which process will be directly affected?
If a cell cannot perform transcription, which process will be directly affected?
According to the central dogma, what molecule is directly responsible for carrying genetic information from the nucleus to the ribosome for protein synthesis?
According to the central dogma, what molecule is directly responsible for carrying genetic information from the nucleus to the ribosome for protein synthesis?
Which base pairing is likely to happen during transcription?
Which base pairing is likely to happen during transcription?
What is the significance of the 5' to 3' directionality in DNA sequencing?
What is the significance of the 5' to 3' directionality in DNA sequencing?
What structural factor contributes to DNA's capacity to store an enormous amount of genetic information?
What structural factor contributes to DNA's capacity to store an enormous amount of genetic information?
What modification to Avery, MacLeod, and McCarty’s experiment would definitively confirm DNA as the transforming principle?
What modification to Avery, MacLeod, and McCarty’s experiment would definitively confirm DNA as the transforming principle?
Given a single strand of DNA with the sequence 5'-GTCG-3', what would be the sequence of its complementary strand?
Given a single strand of DNA with the sequence 5'-GTCG-3', what would be the sequence of its complementary strand?
Considering the requirements for accurate DNA replication, why is base-pairing fidelity essential?
Considering the requirements for accurate DNA replication, why is base-pairing fidelity essential?
In the absence of proteins, which cellular process would be most severely affected according to the universal features of life?
In the absence of proteins, which cellular process would be most severely affected according to the universal features of life?
What did Griffith observe in his experiment that suggested the occurrence of transformation?
What did Griffith observe in his experiment that suggested the occurrence of transformation?
In what way is DNA replication regarded as ‘templated’?
In what way is DNA replication regarded as ‘templated’?
What type of bond links the individual nucleotides together to form a strand of DNA?
What type of bond links the individual nucleotides together to form a strand of DNA?
Why did Avery, MacLeod, and McCarty use enzymes in their experiments?
Why did Avery, MacLeod, and McCarty use enzymes in their experiments?
If a segment of DNA has 100 base pairs, how many nucleotides are present?
If a segment of DNA has 100 base pairs, how many nucleotides are present?
Which of the following experimental steps was crucial in Avery, MacLeod, and McCarty's experiment to identify the 'transforming principle'?
Which of the following experimental steps was crucial in Avery, MacLeod, and McCarty's experiment to identify the 'transforming principle'?
What key observation from Fred Griffith's experiment led to the principle of transformation in bacteria?
What key observation from Fred Griffith's experiment led to the principle of transformation in bacteria?
What is the primary function of DNA?
What is the primary function of DNA?
How does the double helix structure of DNA contribute to its function?
How does the double helix structure of DNA contribute to its function?
If a segment of DNA contains 30% adenine, what percentage of cytosine would be expected?
If a segment of DNA contains 30% adenine, what percentage of cytosine would be expected?
Why is the concept of 'templated polymerization' critical to DNA replication?
Why is the concept of 'templated polymerization' critical to DNA replication?
What would be the direct consequence if a cell could not perform transcription?
What would be the direct consequence if a cell could not perform transcription?
What is the role of mRNA in executing the instructions encoded in DNA?
What is the role of mRNA in executing the instructions encoded in DNA?
What is the significance of the 5' to 3' directionality during DNA replication?
What is the significance of the 5' to 3' directionality during DNA replication?
How does the structure of DNA allow for its enormous capacity to store genetic information?
How does the structure of DNA allow for its enormous capacity to store genetic information?
What is the expected outcome if DNA ligase is non-functional during DNA replication?
What is the expected outcome if DNA ligase is non-functional during DNA replication?
Which of the following is a direct application of understanding the central dogma?
Which of the following is a direct application of understanding the central dogma?
During transcription, which base in RNA pairs with adenine in DNA?
During transcription, which base in RNA pairs with adenine in DNA?
What role do proteins serve as catalysts in the universal features of life?
What role do proteins serve as catalysts in the universal features of life?
Which universal feature of cells ensures accurate hereditary information transfer between generations?
Which universal feature of cells ensures accurate hereditary information transfer between generations?
If a mutation prevented the formation of ribosomes, what process would be most directly affected?
If a mutation prevented the formation of ribosomes, what process would be most directly affected?
In bacterial cells lacking the enzyme DNA gyrase, what would be the most likely consequence during DNA replication?
In bacterial cells lacking the enzyme DNA gyrase, what would be the most likely consequence during DNA replication?
According to the universal features of life, what would happen if a cell’s proteins lost their function?
According to the universal features of life, what would happen if a cell’s proteins lost their function?
How do the base pairs in a DNA double helix contribute to its overall stability?
How do the base pairs in a DNA double helix contribute to its overall stability?
Which statement accurately describes the relationship between genes and proteins?
Which statement accurately describes the relationship between genes and proteins?
If a drug inhibited the enzyme aminoacyl-tRNA synthetase, what cellular process would be most affected?
If a drug inhibited the enzyme aminoacyl-tRNA synthetase, what cellular process would be most affected?
What structural feature is primarily responsible for DNA strands forming a double helix, rather than remaining single strands?
What structural feature is primarily responsible for DNA strands forming a double helix, rather than remaining single strands?
What is the direct impact on protein synthesis if transfer RNA (tRNA) is unable to bind to ribosomes?
What is the direct impact on protein synthesis if transfer RNA (tRNA) is unable to bind to ribosomes?
During DNA replication, what is the role of single-stranded binding proteins (SSB)?
During DNA replication, what is the role of single-stranded binding proteins (SSB)?
Why is proofreading activity an essential feature of DNA polymerases?
Why is proofreading activity an essential feature of DNA polymerases?
The main text for this module is _Molecular Biology of the _______ by Bruce Alberts.
The main text for this module is _Molecular Biology of the _______ by Bruce Alberts.
Characteristics passed from parent to offspring are known as ______ traits.
Characteristics passed from parent to offspring are known as ______ traits.
The hereditary material identified in the middle of the 20th century, encoding an organism's phenotype, is ______.
The hereditary material identified in the middle of the 20th century, encoding an organism's phenotype, is ______.
Fred Griffith’s experiments involved working with different strains of ______.
Fred Griffith’s experiments involved working with different strains of ______.
Griffith's experiments led to the discovery of ______ as the heritable factor.
Griffith's experiments led to the discovery of ______ as the heritable factor.
Avery, Macleod, and McCarty found virulence was transferred via ______.
Avery, Macleod, and McCarty found virulence was transferred via ______.
The molecule that carries the heritable information is ______.
The molecule that carries the heritable information is ______.
A DNA molecule consists of two ______ chains of nucleotides.
A DNA molecule consists of two ______ chains of nucleotides.
In eukaryotes, DNA is enclosed in a cell ______, unlike in prokaryotes.
In eukaryotes, DNA is enclosed in a cell ______, unlike in prokaryotes.
In DNA, there are 4 nucleotides: adenine, guanine, ______, and thymine.
In DNA, there are 4 nucleotides: adenine, guanine, ______, and thymine.
The process of creating RNA from DNA is called ______.
The process of creating RNA from DNA is called ______.
After transcription, the process of creating proteins from RNA is called ______.
After transcription, the process of creating proteins from RNA is called ______.
The ______ states that information cannot go from Protein to DNA or RNA
The ______ states that information cannot go from Protein to DNA or RNA
All cells store their hereditary information in the form of double-strand ______ molecules.
All cells store their hereditary information in the form of double-strand ______ molecules.
Cells replicate their hereditary information by ______ polymerization.
Cells replicate their hereditary information by ______ polymerization.
______ act as Catalysts.
______ act as Catalysts.
Cells transcribe their DNA into ______.
Cells transcribe their DNA into ______.
All cells translate RNA into ______.
All cells translate RNA into ______.
Each protein is encoded by a specific ______
Each protein is encoded by a specific ______
The structure of DNA double helix is held together by ______ bonds.
The structure of DNA double helix is held together by ______ bonds.
DNA is replicated by ______ polymerisation’ replication of dsDNA. The Meselson-Stahl experiment.
DNA is replicated by ______ polymerisation’ replication of dsDNA. The Meselson-Stahl experiment.
In terms of the structure of DNA, a purine (A or G) always base pairs with a ______(T or C).
In terms of the structure of DNA, a purine (A or G) always base pairs with a ______(T or C).
A DNA molecule consists of two complementary chains of ______.
A DNA molecule consists of two complementary chains of ______.
Each protein is encoded by a specific [BLANK].
Each protein is encoded by a specific [BLANK].
Flashcards
Central dogma key elements
Central dogma key elements
The key components are replication, transcription, and translation.
What are heritable traits?
What are heritable traits?
Traits passed from parents to offspring
What is a Phenotype?
What is a Phenotype?
The observable characteristics of an organism.
Who is Fred Griffith?
Who is Fred Griffith?
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Who are Avery, Macleod, and McCarty?
Who are Avery, Macleod, and McCarty?
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What is a nucleotide?
What is a nucleotide?
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Four DNA nucleotides
Four DNA nucleotides
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What is double-stranded DNA?
What is double-stranded DNA?
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DNA strands are?
DNA strands are?
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How are DNA strands held together?
How are DNA strands held together?
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DNA strand
DNA strand
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What is translation?
What is translation?
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Main dogma of molecular biology.
Main dogma of molecular biology.
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What is transcription?
What is transcription?
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What is DNA Replication?
What is DNA Replication?
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How are proteins encoded?
How are proteins encoded?
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How if hereditary info stored?
How if hereditary info stored?
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Levine discovery?
Levine discovery?
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What is phosphodiester bonds?
What is phosphodiester bonds?
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What is Chargaff's rule?
What is Chargaff's rule?
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Basic molecules of life
Basic molecules of life
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Define Heritable Traits
Define Heritable Traits
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What did Avery discover?
What did Avery discover?
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What are the 4 DNA bases?
What are the 4 DNA bases?
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Define DNA Replication
Define DNA Replication
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Double Stranded DNA
Double Stranded DNA
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Transcription
Transcription
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What do cells use as catalysts?
What do cells use as catalysts?
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How does DNA replicate?
How does DNA replicate?
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Study Notes
- The BMS1047 module provides a basic comprehension of genetics and molecular biology
- The module introduces fundamental molecular biology techniques
- The course focusses on prokaryotic organisms
Lecturing Team
- The Molecular Biology and Genetics lecturing team includes Graham Stewart, Sarah Bailey, Suzie Hingley-Wilson, Jenny Ritchie, Yashwanth Subbannayya, and Dany Beste.
Learning Outcomes
- Describe the key elements of the central dogma of molecular biology
- Describe the basic concept of genetic heredity
- Describe genomes and plasmid/chromosome structures
- Describe how DNA is replicated
- Describe how RNA is produced, processed, and proteins synthesized
- Describe basic control of gene expression
- Describe basic techniques in molecular biology and their applications
- Undertake quantitative experiments investigating gene transfer in bacteria
- Undertake qualitative experiments to amplify DNA from a gene and perform basic bioinformatic analysis of DNA sequence
Timetable
- Module Introduction and "Central Dogma" of Molecular Genetics (GS) occurs Wed 03/02/2025 1-2pm in AP3
- Gene Structure (SGB) occurs Wed 05/02/2025 10-11am in AP3
- Molecular Biology techniques1- DNA analysis, PCR, Sequencing (S H-W) occurs Mon 10/02/2025 9-10am in AP3
- Molecular Biology techniques2- RNA and protein analysis (S H-W) occurs Mon 10/02/2025 3-4pm in 03 MS 01
- GROUP A. Practical 1 (JR/GS) occurs Tue 11/02/2025 1-5pm in 01 IFH 00
- Horizontal Gene Transfer (JR) occurs Fri 14/02/2025 10-11am in 03 MS 01
- GROUP B. Practical 1 (JR/GS) occurs Fri 14/02/2025 1-5pm in 01 IFH 00
- Bioinformatics (YS) occurs Mon 17/02/2025 3-4pm in 03 MS 01
- Bioinformatics (YS) occurs Tue 18/02/2025 1-2pm in AP3
- GROUP A. Practical 2 (JR/GS) occurs Tue 18/02/2025 2-5pm in 01 IFH 00
- GROUP B. Practica2 1 (JR/GS) occurs Fri 21/02/2025 2-5pm in 01 IFH 00
- Bioinformatics (YS) occurs Mon 24/02/2025 1-2pm in AP3
- Bioinformatics (YS) occurs Mon 24/02/2025 3-4pm in 03 MS 01
- GROUP A. Practical 3 (JR/GS) occurs Tue 25/02/2025 1-5pm in Online
- GROUP B. Practical 3 (JR/GS) occurs Fri 28/02/2025 1-5pm in 01 IFH 00
- Grp A. Tutorial/Qu and Answer for Practical write-up (JR/GS) occurs Mon 03/03/2025 5-6pm in Online
- DNA replication (prokaryotes) (SGB) occurs Tues 04/03/2025 1-2pm in AP3
- Replication (prokaryotes) (SGB) occurs Thurs 06/03/2025 9-10am in 03 MS 01
- Transcription mechanism and regulation (prokaryotes) (SGB) occurs Fri 07/03/2025 9-11am in AP3
- GROUP A. Writing workshop (SGB) occurs Fri 07/03/2025 11-12pm in 01 AC 01
- Grp B. Tutorial/Qu and Answer for Practical write-up (JR/GS) occurs Fri 07/03/2025 4-5pm in Online
- GROUP B. Writing workshop (SGB) occurs Fri 14/03/2025 2-3pm in LTG
- Molecular Biology techniques3- (S H-W) occurs Fri 14/03/2025 4-6pm in AP3
- Translation (prokaryotes) (DB) occurs Wed 19/03/2025 10-11am in AP3
- GROUP A. Writing workshop (SGB) occurs Fri 21/03/2025 11am-12 in 01 AC 01
- GROUP B. Writing workshop (SGB) occurs Fri 28/03/2025 9am-10 in LTG
- GROUP A. Practical 4 (SGB) occurs Thurs 03/04/2025 10am-1pm in 01 IFH 00
- GROUP B. Practical 4 (SGB) occurs Thurs 03/04/2025 2-5pm in 01 IFH 00
- GROUP A. Practical 4 finish (SGB) occurs Tue 08/04/2025 2-3pm in 01 IFH 00
- GROUP B. Practical 4 finish (SGB) occurs Tue 08/04/2025 3-4pm in 01 IFH 00
- Introduction to eukaryotic genetics (YS) occurs Wed 09/04/2025 9-11am in AP3
- Drop in for essay writing help (SGB) occurs Tues 06/05/2025 12-1pm in AP3
- Revision (GS,JR, SGB, YS, DB) occurs Tues 13/05/2025 10am-12 in 03 MS 01
Assessment
- Coursework: Practical Report 35%
- Coursework: Short essay is 15%
- Online (Open Book) MCQ Exam is 50% within 4-Hr Window (90 Minutes)
Reading
Molecular Biology of the Cell by Bruce Alberts is a key text. Diagrams in lectures are taken from the book
- Molecular Cell Biology by Harvey Lodish and Understanding bioinformatics by Marketa Zvelebil and Jeremy Baum are recommended further texts.
Practicals
- Practicals 1, 2 and 3 involve phenotypic and genotypic investigation of antimicrobial resistance gene transfer to bacteria via conjugation.
- Group 1A day 1 is Tues 11th Feb 1-5 pm, day 2 is Tues 18th Feb 2-5 pm, day 3 is Tues 25th Feb 1-5 pm, tutorial is Mon 3rd March 5-6 pm, Submission deadline Tues 11th March @ 4pm
- Group 1B day 1 is Fri 14th Feb 1-5 pm, day 2 is Fri 21st Feb 2-5 pm, day 3 is Fri 28th Feb 1-5 pm, tutorial is Fri 7th March 4-5 pm, Submission deadline, Fri 14th March @ 4pm
- Do not move between groups, you must complete all sessions in the same group Practical 4+ involves Transformation and blue/white colony screening in E.coli
- Group A: Day 1 is 03/04/25 10am-1pm, Day 2 is 08/04/25 2-3pm
- Group B: Day 1 is 03/04/25 2pm-5pm, Day 2 is 08/04/25 3-4pm
The Central Dogma of Molecular Biology
- All living things are made of Carbohydrates, Lipids, Proteins, and Nucleic acids
- Differences between organisms are heritable.
- Heritable traits: Characteristics passed from parent to offspring
- phenotype: other term for these characteristics
- Experiments on streptococci by Fred Griffith led to the discovery of DNA as the heritable factor.
- Griffith did not see it come to light, he died in 1941
- Avery, Macleod and McCarty discovered virulence can be transferred from dead strain via DNA
- Molecules encoding an organism's phenotype were identified in the middle of the 20th century
- A DNA Molecule Consists of Two Complementary Chains of Nucleotides
- The Structure of DNA Provides a Mechanism for Heredity
- DNA is not enclosed in the cell nucleus of prokaryotes, as is the case for eukaryotes
- The 4 nucleotides in DNA are adenine, guanine, cytosine, and thymine
- Phenotypes/Traits follow genotype
- Antimicrobial resistance (AMR) is arguably the biggest global health threat
- AMR is an inherited trait
- Bacterial virulence is heritable
- There is an enormous potential of DNA to store information
The Central Dogma
- DNA --> RNA --> Protein
- DNA is synthesized through replication
- RNA is synthesized through transcription
- Protein is synthesized through translation
- Francis Crick's Central Dogma was in 1956
Universal Features of Life on Earth
- All Cells Store Their Hereditary Information in the Form of Double-Strand DNA Molecules
- All Cells Replicate Their Hereditary Information by Templated Polymerization
- Semi-conservative or “templated polymerisation” replication of dsDNA
- visualized simply in bacteria because they are unicellular with a single chromosome
- All Cells Transcribe Portions of Their DNA into RNA Molecules
- All Cells Use Proteins as Catalysts
- All Cells Translate RNA into Protein in the Same Way
- Each Protein Is Encoded by a Specific Gene
- Gosling, Franklin, and Wilkins' X-ray diffraction image of DNA provided proof of its helical form
- Phoebus Levene identified there are three components of nucleotides in the early 20th century also discovered DNA was a polynucleotide
- DNA sequence is always wrote starting at the 5' end
- Chargaff's rule, states that a double stranded molecule has percentage base pair equality: A% = T% and G% = C%.
Key Concepts
- Nucleoside: base + sugar
- Nucleotide: base + sugar + phosphate
- Double stranded DNA is helical and has a major and a minor groove
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