BMS1047: Genetics and Molecular Biology

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Questions and Answers

What is the central dogma of molecular biology?

  • RNA is transcribed from protein, and DNA is translated from RNA.
  • DNA is replicated into RNA, which is then translated into protein.
  • Protein is replicated into RNA, which is then translated into DNA.
  • DNA is transcribed into RNA, which is then translated into protein. (correct)

What did Avery, MacLeod, and McCarty's experiment demonstrate?

  • Lipids are the key components for transmitting heritable traits.
  • RNA is the molecule responsible for bacterial transformation.
  • Proteins are the primary carriers of genetic information.
  • DNA is the molecule responsible for bacterial transformation. (correct)

What is the role of proteins in the universal features of life?

  • They replicate DNA.
  • They store hereditary information.
  • They transcribe DNA into RNA.
  • They act as catalysts. (correct)

How does the structure of DNA provide a mechanism for heredity?

<p>DNA's double helix structure with complementary base pairing allows for accurate replication and information storage. (B)</p> Signup and view all the answers

What is the significance of the experiment conducted by Griffith using Streptococcus pneumoniae?

<p>It showed that a 'transforming principle' could change non-virulent bacteria into virulent bacteria. (D)</p> Signup and view all the answers

Which of the following is a characteristic exclusive to eukaryotic DNA?

<p>It is enclosed in a cell nucleus. (C)</p> Signup and view all the answers

Which concept did the experiments conducted by Avery, MacLeod, and McCarty clarify about genetic material?

<p>DNA is the molecule that carries heritable information. (D)</p> Signup and view all the answers

What is the key feature of DNA's double helix structure that allows it to be accurately replicated?

<p>The complementary base pairing between the two strands. (D)</p> Signup and view all the answers

What insight did the work of Phoebus Levene provide regarding the structure of DNA?

<p>The three major components of a nucleotide and the polymeric nature of DNA. (A)</p> Signup and view all the answers

If a double-stranded DNA molecule globally contains 20% guanine, what percentage of adenine would it contain?

<p>$30%$ (C)</p> Signup and view all the answers

What is the primary significance of semi-conservative DNA replication?

<p>It yields two DNA molecules, each with one original and one new strand. (D)</p> Signup and view all the answers

According to the universal features of life on Earth, how do cells replicate their hereditary information?

<p>By templated polymerization. (A)</p> Signup and view all the answers

What structural feature is unique to RNA compared to DNA?

<p>The presence of uracil (U) instead of thymine (T) (C)</p> Signup and view all the answers

Which of the following represents the correct order of the central dogma of molecular biology?

<p>DNA → RNA → Protein (C)</p> Signup and view all the answers

What would be the most likely structural consequence if a purine-purine base pairing occurred within a DNA double helix?

<p>The consistent width of the double helix will be disrupted, causing structural deformities. (A)</p> Signup and view all the answers

What is the relationship between a gene and a protein according to the universal features of life?

<p>Each protein is encoded by a specific gene. (A)</p> Signup and view all the answers

During DNA replication, what critical feature ensures the accurate transmission of hereditary information?

<p>The complementary base pairing during replication. (A)</p> Signup and view all the answers

What role do proteins play in the context of the universal features of cellular life?

<p>Catalysis (C)</p> Signup and view all the answers

Which principle did Griffith's experiment with Streptococcus pneumoniae reveal about heredity?

<p>Genetic material can be transferred between different bacterial strains. (B)</p> Signup and view all the answers

In the context of molecular biology, what is meant by 'templated polymerization' in DNA replication’?

<p>Assembly of a new strand based on the sequence of a template strand. (C)</p> Signup and view all the answers

Which outcome accurately describes the result observed in Avery, Macleod, and McCarty’s experiments?

<p>Digesting the cell extract with DNase prevented the transformation of non-virulent bacteria into virulent bacteria. (B)</p> Signup and view all the answers

What is the significance of Chargaff's rules in the context of DNA structure?

<p>The amount of adenine is equal to thymine, and the amount of guanine is equal to cytosine ($A=T$ and $G=C$). (B)</p> Signup and view all the answers

What is the critical role of complementary base pairing in the double helix structure of DNA?

<p>It facilitates accurate DNA replication and transcription. (A)</p> Signup and view all the answers

Why is DNA replication considered a semi-conservative process?

<p>Each newly formed DNA molecule contains one original and one newly synthesized strand. (B)</p> Signup and view all the answers

If a cell cannot perform transcription, which process will be directly affected?

<p>Protein synthesis (B)</p> Signup and view all the answers

According to the central dogma, what molecule is directly responsible for carrying genetic information from the nucleus to the ribosome for protein synthesis?

<p>mRNA (B)</p> Signup and view all the answers

Which base pairing is likely to happen during transcription?

<p>A with U (B)</p> Signup and view all the answers

What is the significance of the 5' to 3' directionality in DNA sequencing?

<p>It dictates the order in which nucleotides are added during DNA synthesis. (C)</p> Signup and view all the answers

What structural factor contributes to DNA's capacity to store an enormous amount of genetic information?

<p>The sequence of nucleotide bases (C)</p> Signup and view all the answers

What modification to Avery, MacLeod, and McCarty’s experiment would definitively confirm DNA as the transforming principle?

<p>Using a DNase to degrade DNA within the extract. (C)</p> Signup and view all the answers

Given a single strand of DNA with the sequence 5'-GTCG-3', what would be the sequence of its complementary strand?

<p>3'-CGAC-5' (C)</p> Signup and view all the answers

Considering the requirements for accurate DNA replication, why is base-pairing fidelity essential?

<p>To ensure the accurate transmission of genetic information to daughter cells. (D)</p> Signup and view all the answers

In the absence of proteins, which cellular process would be most severely affected according to the universal features of life?

<p>Catalysis of biochemical reactions (B)</p> Signup and view all the answers

What did Griffith observe in his experiment that suggested the occurrence of transformation?

<p>A mixture of heat-killed S strain and live R strain bacteria caused disease in mice. (B)</p> Signup and view all the answers

In what way is DNA replication regarded as ‘templated’?

<p>Each existing strand is a template for creating a new complementary strand. (C)</p> Signup and view all the answers

What type of bond links the individual nucleotides together to form a strand of DNA?

<p>Phosphodiester bond (D)</p> Signup and view all the answers

Why did Avery, MacLeod, and McCarty use enzymes in their experiments?

<p>To selectively degrade nucleic acids, proteins, and other molecules (B)</p> Signup and view all the answers

If a segment of DNA has 100 base pairs, how many nucleotides are present?

<p>200 (A)</p> Signup and view all the answers

Which of the following experimental steps was crucial in Avery, MacLeod, and McCarty's experiment to identify the 'transforming principle'?

<p>Fractionating cell extracts and testing each fraction for transformation activity. (D)</p> Signup and view all the answers

What key observation from Fred Griffith's experiment led to the principle of transformation in bacteria?

<p>Non-virulent bacteria became virulent when mixed with heat-killed virulent bacteria. (B)</p> Signup and view all the answers

What is the primary function of DNA?

<p>Storing and transmitting genetic information. (C)</p> Signup and view all the answers

How does the double helix structure of DNA contribute to its function?

<p>It provides a mechanism for accurate replication and information storage. (A)</p> Signup and view all the answers

If a segment of DNA contains 30% adenine, what percentage of cytosine would be expected?

<p>20% (C)</p> Signup and view all the answers

Why is the concept of 'templated polymerization' critical to DNA replication?

<p>It guarantees the accurate copying of the genetic information. (B)</p> Signup and view all the answers

What would be the direct consequence if a cell could not perform transcription?

<p>The cell would not be able to synthesize proteins. (D)</p> Signup and view all the answers

What is the role of mRNA in executing the instructions encoded in DNA?

<p>It carries genetic information from the nucleus to the ribosome. (A)</p> Signup and view all the answers

What is the significance of the 5' to 3' directionality during DNA replication?

<p>It ensures that DNA polymerase can only add nucleotides to the 3' hydroxyl group. (C)</p> Signup and view all the answers

How does the structure of DNA allow for its enormous capacity to store genetic information?

<p>The sequence of base pairs can vary greatly, allowing for many different combinations. (A)</p> Signup and view all the answers

What is the expected outcome if DNA ligase is non-functional during DNA replication?

<p>Okazaki fragments would not be joined together. (D)</p> Signup and view all the answers

Which of the following is a direct application of understanding the central dogma?

<p>Developing methods for DNA sequencing. (B)</p> Signup and view all the answers

During transcription, which base in RNA pairs with adenine in DNA?

<p>Uracil (B)</p> Signup and view all the answers

What role do proteins serve as catalysts in the universal features of life?

<p>Accelerating biochemical reactions (C)</p> Signup and view all the answers

Which universal feature of cells ensures accurate hereditary information transfer between generations?

<p>DNA replication (A)</p> Signup and view all the answers

If a mutation prevented the formation of ribosomes, what process would be most directly affected?

<p>Translation (D)</p> Signup and view all the answers

In bacterial cells lacking the enzyme DNA gyrase, what would be the most likely consequence during DNA replication?

<p>Increased supercoiling ahead of the replication fork. (B)</p> Signup and view all the answers

According to the universal features of life, what would happen if a cell’s proteins lost their function?

<p>The cell will be unable to catalyze necessary biochemical reactions. (B)</p> Signup and view all the answers

How do the base pairs in a DNA double helix contribute to its overall stability?

<p>Hydrogen bonds between the bases. (C)</p> Signup and view all the answers

Which statement accurately describes the relationship between genes and proteins?

<p>A gene encodes a specific protein (or polypeptide chain). (C)</p> Signup and view all the answers

If a drug inhibited the enzyme aminoacyl-tRNA synthetase, what cellular process would be most affected?

<p>Attachment of amino acids to tRNA. (A)</p> Signup and view all the answers

What structural feature is primarily responsible for DNA strands forming a double helix, rather than remaining single strands?

<p>Hydrogen bonding between complementary base pairs (A)</p> Signup and view all the answers

What is the direct impact on protein synthesis if transfer RNA (tRNA) is unable to bind to ribosomes?

<p>Elongation of the polypeptide chain would cease. (A)</p> Signup and view all the answers

During DNA replication, what is the role of single-stranded binding proteins (SSB)?

<p>To prevent the re-annealing of separated DNA strands (C)</p> Signup and view all the answers

Why is proofreading activity an essential feature of DNA polymerases?

<p>To prevent mutations by correcting errors during DNA replication (D)</p> Signup and view all the answers

The main text for this module is _Molecular Biology of the _______ by Bruce Alberts.

<p>Cell</p> Signup and view all the answers

Characteristics passed from parent to offspring are known as ______ traits.

<p>heritable</p> Signup and view all the answers

The hereditary material identified in the middle of the 20th century, encoding an organism's phenotype, is ______.

<p>DNA</p> Signup and view all the answers

Fred Griffith’s experiments involved working with different strains of ______.

<p>Streptococci</p> Signup and view all the answers

Griffith's experiments led to the discovery of ______ as the heritable factor.

<p>DNA</p> Signup and view all the answers

Avery, Macleod, and McCarty found virulence was transferred via ______.

<p>DNA</p> Signup and view all the answers

The molecule that carries the heritable information is ______.

<p>DNA</p> Signup and view all the answers

A DNA molecule consists of two ______ chains of nucleotides.

<p>complementary</p> Signup and view all the answers

In eukaryotes, DNA is enclosed in a cell ______, unlike in prokaryotes.

<p>nucleus</p> Signup and view all the answers

In DNA, there are 4 nucleotides: adenine, guanine, ______, and thymine.

<p>cytosine</p> Signup and view all the answers

The process of creating RNA from DNA is called ______.

<p>transcription</p> Signup and view all the answers

After transcription, the process of creating proteins from RNA is called ______.

<p>translation</p> Signup and view all the answers

The ______ states that information cannot go from Protein to DNA or RNA

<p>Central Dogma</p> Signup and view all the answers

All cells store their hereditary information in the form of double-strand ______ molecules.

<p>DNA</p> Signup and view all the answers

Cells replicate their hereditary information by ______ polymerization.

<p>templated</p> Signup and view all the answers

______ act as Catalysts.

<p>Proteins</p> Signup and view all the answers

Cells transcribe their DNA into ______.

<p>RNA</p> Signup and view all the answers

All cells translate RNA into ______.

<p>Protein</p> Signup and view all the answers

Each protein is encoded by a specific ______

<p>gene</p> Signup and view all the answers

The structure of DNA double helix is held together by ______ bonds.

<p>hydrogen</p> Signup and view all the answers

DNA is replicated by ______ polymerisation’ replication of dsDNA. The Meselson-Stahl experiment.

<p>templated</p> Signup and view all the answers

In terms of the structure of DNA, a purine (A or G) always base pairs with a ______(T or C).

<p>pyrimidine</p> Signup and view all the answers

A DNA molecule consists of two complementary chains of ______.

<p>nucleotides</p> Signup and view all the answers

Each protein is encoded by a specific [BLANK].

<p>gene</p> Signup and view all the answers

Flashcards

Central dogma key elements

The key components are replication, transcription, and translation.

What are heritable traits?

Traits passed from parents to offspring

What is a Phenotype?

The observable characteristics of an organism.

Who is Fred Griffith?

He demonstrated genetic transformation using Streptococcus pneumoniae.

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Who are Avery, Macleod, and McCarty?

Avery, Macleod, and McCarty proved that DNA transfers virulence.

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What is a nucleotide?

It consists of a phosphate group, sugar, and a nucleobase (A, T, C, G).

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Four DNA nucleotides

Adenine, guanine, cytosine, and thymine

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What is double-stranded DNA?

DNA consists of two strands, in the form of a double helix.

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DNA strands are?

Strands run in opposite directions

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How are DNA strands held together?

The two strands of the DNA double helix are held together by hydrogen bonds

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DNA strand

A strand of DNA with four repeating units

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What is translation?

It is the process by which cells make proteins

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Main dogma of molecular biology.

DNA to RNA to protein

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What is transcription?

Information flow from DNA -> RNA

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What is DNA Replication?

Using an existing DNA as a template

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How are proteins encoded?

Each protein is encoded by a specific gene

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How if hereditary info stored?

Cells store their hereditary information as DNA

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Levine discovery?

Levene identified the three components of nucleotides

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What is phosphodiester bonds?

They join nucleotides in nucleic acids

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What is Chargaff's rule?

His rule holds that a double stranded DNA molecule has percentage base pair

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Basic molecules of life

The four basic molecules of life are carbohydrates, lipids, proteins, and nucleic acids.

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Define Heritable Traits

Traits that are passed down (inherited) from parent to offspring.

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What did Avery discover?

It was discovered that virulence was transferred via DNA.

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What are the 4 DNA bases?

Adenine (A), Guanine (G), Cytosine (C), Thymine (T).

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Define DNA Replication

The process where cells copy their DNA.

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Double Stranded DNA

Double-stranded DNA is helical and has a major and minor groove.

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Transcription

Cells transcribe portions of their DNA into RNA molecules

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What do cells use as catalysts?

Cells use proteins as catalysts

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How does DNA replicate?

DNA is replicated by templated polymerisation

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Study Notes

  • The BMS1047 module provides a basic comprehension of genetics and molecular biology
  • The module introduces fundamental molecular biology techniques
  • The course focusses on prokaryotic organisms

Lecturing Team

  • The Molecular Biology and Genetics lecturing team includes Graham Stewart, Sarah Bailey, Suzie Hingley-Wilson, Jenny Ritchie, Yashwanth Subbannayya, and Dany Beste.

Learning Outcomes

  • Describe the key elements of the central dogma of molecular biology
  • Describe the basic concept of genetic heredity
  • Describe genomes and plasmid/chromosome structures
  • Describe how DNA is replicated
  • Describe how RNA is produced, processed, and proteins synthesized
  • Describe basic control of gene expression
  • Describe basic techniques in molecular biology and their applications
  • Undertake quantitative experiments investigating gene transfer in bacteria
  • Undertake qualitative experiments to amplify DNA from a gene and perform basic bioinformatic analysis of DNA sequence

Timetable

  • Module Introduction and "Central Dogma" of Molecular Genetics (GS) occurs Wed 03/02/2025 1-2pm in AP3
  • Gene Structure (SGB) occurs Wed 05/02/2025 10-11am in AP3
  • Molecular Biology techniques1- DNA analysis, PCR, Sequencing (S H-W) occurs Mon 10/02/2025 9-10am in AP3
  • Molecular Biology techniques2- RNA and protein analysis (S H-W) occurs Mon 10/02/2025 3-4pm in 03 MS 01
  • GROUP A. Practical 1 (JR/GS) occurs Tue 11/02/2025 1-5pm in 01 IFH 00
  • Horizontal Gene Transfer (JR) occurs Fri 14/02/2025 10-11am in 03 MS 01
  • GROUP B. Practical 1 (JR/GS) occurs Fri 14/02/2025 1-5pm in 01 IFH 00
  • Bioinformatics (YS) occurs Mon 17/02/2025 3-4pm in 03 MS 01
  • Bioinformatics (YS) occurs Tue 18/02/2025 1-2pm in AP3
  • GROUP A. Practical 2 (JR/GS) occurs Tue 18/02/2025 2-5pm in 01 IFH 00
  • GROUP B. Practica2 1 (JR/GS) occurs Fri 21/02/2025 2-5pm in 01 IFH 00
  • Bioinformatics (YS) occurs Mon 24/02/2025 1-2pm in AP3
  • Bioinformatics (YS) occurs Mon 24/02/2025 3-4pm in 03 MS 01
  • GROUP A. Practical 3 (JR/GS) occurs Tue 25/02/2025 1-5pm in Online
  • GROUP B. Practical 3 (JR/GS) occurs Fri 28/02/2025 1-5pm in 01 IFH 00
  • Grp A. Tutorial/Qu and Answer for Practical write-up (JR/GS) occurs Mon 03/03/2025 5-6pm in Online
  • DNA replication (prokaryotes) (SGB) occurs Tues 04/03/2025 1-2pm in AP3
  • Replication (prokaryotes) (SGB) occurs Thurs 06/03/2025 9-10am in 03 MS 01
  • Transcription mechanism and regulation (prokaryotes) (SGB) occurs Fri 07/03/2025 9-11am in AP3
  • GROUP A. Writing workshop (SGB) occurs Fri 07/03/2025 11-12pm in 01 AC 01
  • Grp B. Tutorial/Qu and Answer for Practical write-up (JR/GS) occurs Fri 07/03/2025 4-5pm in Online
  • GROUP B. Writing workshop (SGB) occurs Fri 14/03/2025 2-3pm in LTG
  • Molecular Biology techniques3- (S H-W) occurs Fri 14/03/2025 4-6pm in AP3
  • Translation (prokaryotes) (DB) occurs Wed 19/03/2025 10-11am in AP3
  • GROUP A. Writing workshop (SGB) occurs Fri 21/03/2025 11am-12 in 01 AC 01
  • GROUP B. Writing workshop (SGB) occurs Fri 28/03/2025 9am-10 in LTG
  • GROUP A. Practical 4 (SGB) occurs Thurs 03/04/2025 10am-1pm in 01 IFH 00
  • GROUP B. Practical 4 (SGB) occurs Thurs 03/04/2025 2-5pm in 01 IFH 00
  • GROUP A. Practical 4 finish (SGB) occurs Tue 08/04/2025 2-3pm in 01 IFH 00
  • GROUP B. Practical 4 finish (SGB) occurs Tue 08/04/2025 3-4pm in 01 IFH 00
  • Introduction to eukaryotic genetics (YS) occurs Wed 09/04/2025 9-11am in AP3
  • Drop in for essay writing help (SGB) occurs Tues 06/05/2025 12-1pm in AP3
  • Revision (GS,JR, SGB, YS, DB) occurs Tues 13/05/2025 10am-12 in 03 MS 01

Assessment

  • Coursework: Practical Report 35%
  • Coursework: Short essay is 15%
  • Online (Open Book) MCQ Exam is 50% within 4-Hr Window (90 Minutes)

Reading

Molecular Biology of the Cell by Bruce Alberts is a key text. Diagrams in lectures are taken from the book

  • Molecular Cell Biology by Harvey Lodish and Understanding bioinformatics by Marketa Zvelebil and Jeremy Baum are recommended further texts.

Practicals

  • Practicals 1, 2 and 3 involve phenotypic and genotypic investigation of antimicrobial resistance gene transfer to bacteria via conjugation.
  • Group 1A day 1 is Tues 11th Feb 1-5 pm, day 2 is Tues 18th Feb 2-5 pm, day 3 is Tues 25th Feb 1-5 pm, tutorial is Mon 3rd March 5-6 pm, Submission deadline Tues 11th March @ 4pm
  • Group 1B day 1 is Fri 14th Feb 1-5 pm, day 2 is Fri 21st Feb 2-5 pm, day 3 is Fri 28th Feb 1-5 pm, tutorial is Fri 7th March 4-5 pm, Submission deadline, Fri 14th March @ 4pm
  • Do not move between groups, you must complete all sessions in the same group Practical 4+ involves Transformation and blue/white colony screening in E.coli
  • Group A: Day 1 is 03/04/25 10am-1pm, Day 2 is 08/04/25 2-3pm
  • Group B: Day 1 is 03/04/25 2pm-5pm, Day 2 is 08/04/25 3-4pm

The Central Dogma of Molecular Biology

  • All living things are made of Carbohydrates, Lipids, Proteins, and Nucleic acids
  • Differences between organisms are heritable.
    • Heritable traits: Characteristics passed from parent to offspring
    • phenotype: other term for these characteristics
  • Experiments on streptococci by Fred Griffith led to the discovery of DNA as the heritable factor.
    • Griffith did not see it come to light, he died in 1941
  • Avery, Macleod and McCarty discovered virulence can be transferred from dead strain via DNA
  • Molecules encoding an organism's phenotype were identified in the middle of the 20th century
  • A DNA Molecule Consists of Two Complementary Chains of Nucleotides
  • The Structure of DNA Provides a Mechanism for Heredity
  • DNA is not enclosed in the cell nucleus of prokaryotes, as is the case for eukaryotes
  • The 4 nucleotides in DNA are adenine, guanine, cytosine, and thymine
  • Phenotypes/Traits follow genotype
  • Antimicrobial resistance (AMR) is arguably the biggest global health threat
    • AMR is an inherited trait
    • Bacterial virulence is heritable
  • There is an enormous potential of DNA to store information

The Central Dogma

  • DNA --> RNA --> Protein
  • DNA is synthesized through replication
  • RNA is synthesized through transcription
  • Protein is synthesized through translation
  • Francis Crick's Central Dogma was in 1956

Universal Features of Life on Earth

  • All Cells Store Their Hereditary Information in the Form of Double-Strand DNA Molecules
  • All Cells Replicate Their Hereditary Information by Templated Polymerization
    • Semi-conservative or “templated polymerisation” replication of dsDNA
    • visualized simply in bacteria because they are unicellular with a single chromosome
  • All Cells Transcribe Portions of Their DNA into RNA Molecules
  • All Cells Use Proteins as Catalysts
  • All Cells Translate RNA into Protein in the Same Way
  • Each Protein Is Encoded by a Specific Gene
  • Gosling, Franklin, and Wilkins' X-ray diffraction image of DNA provided proof of its helical form
  • Phoebus Levene identified there are three components of nucleotides in the early 20th century also discovered DNA was a polynucleotide
  • DNA sequence is always wrote starting at the 5' end
  • Chargaff's rule, states that a double stranded molecule has percentage base pair equality: A% = T% and G% = C%.

Key Concepts

  • Nucleoside: base + sugar
  • Nucleotide: base + sugar + phosphate
  • Double stranded DNA is helical and has a major and a minor groove

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