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Transcription in Prokaryotes

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Questions and Answers

What is the function of the σ subunit in RNA polymerase?

To synthesize the RNA molecule during elongation

To enhance the processivity of transcription

To unwind the DNA double helix during initiation

To recognize specific promoter sites on DNA (correct)

What is the name of the consensus sequence found at position –10 in the promoter region?

GC box

TATA box

CAAT box

Pribnow box (correct)

What is the composition of the RNA polymerase holoenzyme in E. coli?

αββ’ω

α2ββ’ωσ (correct)

αββ’ωσ

α2ββ’ω

During which phase of transcription does RNA polymerase recognize a specific site on the DNA?

Initiation Signup and view all the answers

What is the size of the promoter region in prokaryotes?

40–60 bp Signup and view all the answers

What is the function of the core enzyme in transcription?

To synthesize RNA during elongation Signup and view all the answers

What is the consensus sequence of the –35 promoter region?

TTGACA Signup and view all the answers

What is released from the transcriptional complex during the elongation phase?

The σ factor Signup and view all the answers

What is the direction of RNA synthesis by RNA polymerase?

5’ → 3’ Signup and view all the answers

What is the region of unwound DNA called that is undergoing transcription?

Transcription bubble Signup and view all the answers

What is the complex of RNA polymerase, DNA template, and new RNA transcript called?

Ternary complex Signup and view all the answers

What is the first nucleotide in the RNA transcript usually?

pppG or pppA Signup and view all the answers

Which strand of DNA is used as a template during elongation?

Antisense (-) strand Signup and view all the answers

In what direction is mRNA synthesized during elongation?

5′ to 3′ direction Signup and view all the answers

What is the function of the promoter site in transcription?

To identify the correct strand for transcription Signup and view all the answers

What is the common feature of termination sequences?

GC-rich region followed by an AT-rich sequence Signup and view all the answers

What happens when the RNA polymerase encounters a termination signal?

It stops transcription and releases the RNA transcript Signup and view all the answers

What is required for termination at some termination sites?

The presence of rho protein Signup and view all the answers