Podcast
Questions and Answers
What is the function of the σ subunit in RNA polymerase?
What is the function of the σ subunit in RNA polymerase?
- To synthesize the RNA molecule during elongation
- To enhance the processivity of transcription
- To unwind the DNA double helix during initiation
- To recognize specific promoter sites on DNA (correct)
What is the name of the consensus sequence found at position –10 in the promoter region?
What is the name of the consensus sequence found at position –10 in the promoter region?
- GC box
- TATA box
- CAAT box
- Pribnow box (correct)
What is the composition of the RNA polymerase holoenzyme in E. coli?
What is the composition of the RNA polymerase holoenzyme in E. coli?
- αββ’ω
- α2ββ’ωσ (correct)
- αββ’ωσ
- α2ββ’ω
During which phase of transcription does RNA polymerase recognize a specific site on the DNA?
During which phase of transcription does RNA polymerase recognize a specific site on the DNA?
What is the size of the promoter region in prokaryotes?
What is the size of the promoter region in prokaryotes?
What is the function of the core enzyme in transcription?
What is the function of the core enzyme in transcription?
What is the consensus sequence of the –35 promoter region?
What is the consensus sequence of the –35 promoter region?
What is released from the transcriptional complex during the elongation phase?
What is released from the transcriptional complex during the elongation phase?
What is the direction of RNA synthesis by RNA polymerase?
What is the direction of RNA synthesis by RNA polymerase?
What is the region of unwound DNA called that is undergoing transcription?
What is the region of unwound DNA called that is undergoing transcription?
What is the complex of RNA polymerase, DNA template, and new RNA transcript called?
What is the complex of RNA polymerase, DNA template, and new RNA transcript called?
What is the first nucleotide in the RNA transcript usually?
What is the first nucleotide in the RNA transcript usually?
Which strand of DNA is used as a template during elongation?
Which strand of DNA is used as a template during elongation?
In what direction is mRNA synthesized during elongation?
In what direction is mRNA synthesized during elongation?
What is the function of the promoter site in transcription?
What is the function of the promoter site in transcription?
What is the common feature of termination sequences?
What is the common feature of termination sequences?
What happens when the RNA polymerase encounters a termination signal?
What happens when the RNA polymerase encounters a termination signal?
What is required for termination at some termination sites?
What is required for termination at some termination sites?