Blood Detection Methods Quiz

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Questions and Answers

What is the principle of the Benzidine test?

  • The haem group of haemoglobin possesses a peroxidase-like activity which catalyses the breakdown of hydrogen peroxide (correct)
  • The formation of haemoglobin derivative crystals such as haematin, haemin and haemochromogen
  • The sensitivity of the test to detect bloodstains
  • The reaction of haemoglobin with Nippe’s Reagent

What can cause false positives in crystal tests?

  • Substances other than heme which possess a similar peroxidase-like activity (correct)
  • The sensitivity of the test
  • The presence of haemoglobin derivative crystals
  • Chemical oxidants

What is the sensitivity of crystal tests to detect haemoglobin?

  • About 0.1 mL of blood or 0.01 mg of haemoglobin
  • About 0.01 mL of blood or 1 mg of haemoglobin
  • About 1 mL of blood or 0.001 mg of haemoglobin
  • About 0.001 mL of blood or 0.1 mg of haemoglobin (correct)

What is the composition of Nippe’s Reagent?

<p>0.1gm KCl + 0.1 gm KBr + 0.1 gm KI in 100 ml glacial acetic acid (B)</p> Signup and view all the answers

What is the procedure for the Benzidine test?

<p>Dissolve some benzidine crystals in 0.5 ml of glacial acetic acid, add 1 ml dist. Water + 1 drop blood, add a few drops of H2O2 and observe the color (B)</p> Signup and view all the answers

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