5 Questions
What is the principle of the Benzidine test?
The haem group of haemoglobin possesses a peroxidase-like activity which catalyses the breakdown of hydrogen peroxide
What can cause false positives in crystal tests?
Substances other than heme which possess a similar peroxidase-like activity
What is the sensitivity of crystal tests to detect haemoglobin?
About 0.001 mL of blood or 0.1 mg of haemoglobin
What is the composition of Nippe’s Reagent?
0.1gm KCl + 0.1 gm KBr + 0.1 gm KI in 100 ml glacial acetic acid
What is the procedure for the Benzidine test?
Dissolve some benzidine crystals in 0.5 ml of glacial acetic acid, add 1 ml dist. Water + 1 drop blood, add a few drops of H2O2 and observe the color
Test your knowledge of chemical methods for detecting blood with this quiz. Explore crystal tests, catalytic tests, and instrumental methods that rely on the presence of haemoglobin. Understand the peroxidase-like activity of haemoglobin's haem group and its role in these detection methods.
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