Blood Culture Methods and Techniques Quiz
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Questions and Answers

Which type of organism primarily infects immunosuppressed patients and those with serious or terminal illness?

  • Fungi (correct)
  • Viruses
  • Parasites
  • Bacteria

    • Which type of organism is particularly found in neonates receiving lipid-supplemented parenteral nutrition?

  • Parasites
  • Fungi (correct)
  • Bacteria
  • Viruses

    • Which type of organism can disseminate to all body organs?

  • Bacteria
  • Fungi
  • Parasites (correct)
  • Viruses

    • Which type of organism enters the blood via invasion of lymph nodes and other organs?

    <p>Fungi</p> Signup and view all the answers

    Which type of organism primarily infects the target organ or cells?

    <p>Viruses</p> Signup and view all the answers

    Which type of organism infects the endocardium and is most commonly caused by bacteria?

    <p>Bacteria</p> Signup and view all the answers

    Which agar is used to culture Gram-negative bacteria?

    <p>MacConkey agar</p> Signup and view all the answers

    Which organisms are considered probable contaminants in blood cultures?

    <p>Bacillus anthracis</p> Signup and view all the answers

    Which condition is NOT consistent with sepsis based on clinical presentation?

    <p>Growth of skin flora</p> Signup and view all the answers

    Which microorganisms may require alternative methods for diagnosis if they cause bloodstream infections?

    <p>Brucella spp.</p> Signup and view all the answers

    What is the incubation period for Brucella spp. in blood cultures?

    <p>5-60 days</p> Signup and view all the answers

    When should the physician be notified of positive culture results?

    <p>After 48 hours</p> Signup and view all the answers

    Which factor is NOT a contributing factor to bloodstream infections?

    <p>Immunosuppressive agents</p> Signup and view all the answers

    What is the recommended volume of blood to collect for blood cultures in adults?

    <p>10 to 20 mL per culture</p> Signup and view all the answers

    What is the purpose of using anticoagulants in blood culture collection?

    <p>To prevent clotting</p> Signup and view all the answers

    What is the recommended concentration of sodium polyanethol sulfonate (SPS) as an anticoagulant?

    <p>0.025% to 0.03%</p> Signup and view all the answers

    What is the recommended number of blood cultures to obtain for optimum sensitivity in patients with endocarditis who have not received antibiotics?

    <p>Two blood cultures</p> Signup and view all the answers

    What should phlebotomists do to prevent contamination during blood drawing?

    <p>All of the above</p> Signup and view all the answers

    Which of the following is NOT a component of blood culture media?

    <p>Gelatin</p> Signup and view all the answers

    What is the recommended blood to medium ratio in traditional dilution blood factor?

    <p>1:10</p> Signup and view all the answers

    What is the purpose of venting the bottles in conventional blood cultures?

    <p>To enhance the growth of most aerobic bacteria</p> Signup and view all the answers

    Which method combines liquid and solid medium in the same bottle for easy subculture?

    <p>Biphasic method</p> Signup and view all the answers

    What is the purpose of instrument-based systems like BACTEC and BacT/ALERT?

    <p>To measure the production of CO2 by metabolizing microorganisms</p> Signup and view all the answers

    How are positive blood cultures detected?

    <p>All of the above</p> Signup and view all the answers

    Which term refers to the presence of bacteria in the bloodstream?

    <p>Bacteremia</p> Signup and view all the answers

    What are the clinical manifestations of septicemia?

    <p>All of the above</p> Signup and view all the answers

    Which bacteria are most commonly isolated in bloodstream infections?

    <p>Staphylococcus aureus, Streptococcus pneumoniae, Candida albicans</p> Signup and view all the answers

    What is the purpose of using anticoagulants in blood cultures?

    <p>To prevent blood clotting</p> Signup and view all the answers

    How are probable pathogens distinguished from contaminants in a blood culture?

    <p>By the type of bacteria isolated</p> Signup and view all the answers

    Which term refers to the presence of bacteria in the blood?

    <p>Bacteremia</p> Signup and view all the answers

    What is the suffix 'emia' derived from?

    <p>Greek word for 'blood'</p> Signup and view all the answers

    Which of the following is a clinical manifestation of septicemia?

    <p>All of the above</p> Signup and view all the answers

    Which bacteria is most commonly isolated in bloodstream infections?

    <p>Staphylococcus aureus</p> Signup and view all the answers

    What is the process of specimen collection, handling, processing, and culturing for bacteremia detection called?

    <p>Blood culture</p> Signup and view all the answers

    What are the major types of bloodstream infections?

    <p>Bacteremia and septicemia</p> Signup and view all the answers

    Which organisms may require alternative methods for diagnosis if they cause bloodstream infections?

    <p>Brucella spp.</p> Signup and view all the answers

    What is the incubation period for Brucella spp. in blood cultures?

    <p>5-60 days</p> Signup and view all the answers

    Which agar is used to culture Gram-negative bacteria?

    <p>MacConkey agar</p> Signup and view all the answers

    What is the purpose of venting the bottles in conventional blood cultures?

    <p>To allow aerobic growth of bacteria</p> Signup and view all the answers

    Which type of organism infects the endocardium and is most commonly caused by bacteria?

    <p>Endocarditis</p> Signup and view all the answers

    What is the recommended number of blood cultures to obtain for optimum sensitivity in patients with endocarditis who have not received antibiotics?

    <p>3 sets</p> Signup and view all the answers

    Which type of organism primarily infects immunosuppressed patients and those with serious or terminal illness?

    <p>Opportunistic pathogens</p> Signup and view all the answers

    What is the recommended volume of blood to collect for blood cultures in adults?

    <p>20-30 mL</p> Signup and view all the answers

    What is the purpose of using anticoagulants in blood culture collection?

    <p>To prevent clotting</p> Signup and view all the answers

    Which factor is NOT a contributing factor to bloodstream infections?

    <p>Regular hand hygiene</p> Signup and view all the answers

    Which of the following is a clinical manifestation of septicemia?

    <p>Fever, hypothermia, chills</p> Signup and view all the answers

    What is the purpose of using anticoagulants in blood culture collection?

    <p>To prevent clotting of the blood sample</p> Signup and view all the answers

    Which type of organism primarily infects immunosuppressed patients and those with serious or terminal illness?

    <p>Opportunistic pathogens</p> Signup and view all the answers

    What is the recommended number of blood cultures to obtain for optimum sensitivity in patients with endocarditis who have not received antibiotics?

    <p>3 sets</p> Signup and view all the answers

    Which term refers to the presence of bacteria in the bloodstream?

    <p>Bacteremia</p> Signup and view all the answers

    Which type of organism primarily infects immunosuppressed patients and those with serious or terminal illness?

    <p>Fungi</p> Signup and view all the answers

    Which type of organism enters the blood via invasion of lymph nodes and other organs?

    <p>Parasites</p> Signup and view all the answers

    What is the most common route of entry for bacteremia from an extravascular site?

    <p>Genitourinary tract</p> Signup and view all the answers

    Which type of organism is particularly found in neonates receiving lipid-supplemented parenteral nutrition?

    <p>Fungi</p> Signup and view all the answers

    Which type of organism primarily infects the endocardium and is most commonly caused by bacteria?

    <p>Bacteria</p> Signup and view all the answers

    What is the recommended number of blood cultures to obtain for optimum sensitivity in patients with infective endocarditis who have not received antibiotics?

    <p>Three blood cultures are sufficient</p> Signup and view all the answers

    What is the recommended volume of blood to collect for blood cultures in infants and children?

    <p>3-4 mL</p> Signup and view all the answers

    What is the most commonly used anticoagulant in blood culture collection?

    <p>SPS</p> Signup and view all the answers

    What is the purpose of venting the bottles in conventional blood cultures?

    <p>To prevent clotting</p> Signup and view all the answers

    Which factor is NOT a contributing factor to bloodstream infections?

    <p>Prolonged survival of debilitated and seriously ill patients</p> Signup and view all the answers

    Which of the following is a special culture/culturing condition for Brucella spp. in blood cultures?

    <p>Prolonged incubation period of 5-60 days</p> Signup and view all the answers

    Which of the following is NOT a criterion to distinguish probable pathogens from contaminants in blood cultures?

    <p>Clinical presentation consistent with sepsis</p> Signup and view all the answers

    Which of the following organisms is known as 'nutritionally variant' or 'satelliting' streptococci?

    <p>Streptococci</p> Signup and view all the answers

    Which agar is used to culture Campylobacter in blood cultures?

    <p>CAMPY agar</p> Signup and view all the answers

    Which of the following organisms is NOT considered a probable contaminant in blood cultures?

    <p>Staphylococcus aureus</p> Signup and view all the answers

    Which method combines liquid and solid medium in the same bottle for easy subculture?

    <p>Biphasic method</p> Signup and view all the answers

    What is the purpose of using anticoagulants in blood culture collection?

    <p>To prevent clotting of blood</p> Signup and view all the answers

    Which condition is NOT consistent with sepsis based on clinical presentation?

    <p>Normal white blood cell count</p> Signup and view all the answers

    What is the recommended concentration of sodium polyanethol sulfonate (SPS) as an anticoagulant?

    <p>1:10 ratio</p> Signup and view all the answers

    Which agar is used to culture Gram-negative bacteria?

    <p>MacConkey agar</p> Signup and view all the answers

    Study Notes

    Blood Culture Methods and Techniques

    • Gelatin can counteract the inhibitory action of SPS, but it may decrease the recovery of other organisms.
    • Dilution blood factor: traditionally a 1:10 ratio of blood to medium, but new commercial media with additives may use a 1:5 ratio.
    • Blood culture media typically contain nutrient broth, anticoagulant, and various additives.
    • Some specialized media can absorb and inactivate antimicrobial agents, increasing the yield of microorganisms.
    • Incubation conditions include temperature and time, with longer incubation required for certain microorganisms.
    • Conventional blood cultures use commercially prepared bottles with low oxidation-reduction potential.
    • Venting the bottles may be necessary for growth of obligate aerobes.
    • Constant agitation during the first 24 hours enhances the growth of most aerobic bacteria.
    • Bottles are examined daily for growth indicators like turbidity, gas, and discoloration.
    • The biphasic method combines liquid and solid medium in the same bottle, allowing for easy subculture.
    • Lysis centrifugation is a method used to detect microorganisms in body fluid specimens.
    • Instrument-based systems like BACTEC and BacT/ALERT measure the production of CO2 by metabolizing microorganisms.

    Handling Positive Blood Cultures:

    • Positive blood cultures are detected through appearance, subculture plates, or instrument-based detection.
    • Gram-stained smears and other tests are performed to identify and test antimicrobial susceptibility.

    Blood Culture Methods and Techniques

    • Gelatin can counteract the inhibitory action of SPS, but it may decrease the recovery of other organisms.
    • Dilution blood factor: traditionally a 1:10 ratio of blood to medium, but new commercial media with additives may use a 1:5 ratio.
    • Blood culture media typically contain nutrient broth, anticoagulant, and various additives.
    • Some specialized media can absorb and inactivate antimicrobial agents, increasing the yield of microorganisms.
    • Incubation conditions include temperature and time, with longer incubation required for certain microorganisms.
    • Conventional blood cultures use commercially prepared bottles with low oxidation-reduction potential.
    • Venting the bottles may be necessary for growth of obligate aerobes.
    • Constant agitation during the first 24 hours enhances the growth of most aerobic bacteria.
    • Bottles are examined daily for growth indicators like turbidity, gas, and discoloration.
    • The biphasic method combines liquid and solid medium in the same bottle, allowing for easy subculture.
    • Lysis centrifugation is a method used to detect microorganisms in body fluid specimens.
    • Instrument-based systems like BACTEC and BacT/ALERT measure the production of CO2 by metabolizing microorganisms.

    Handling Positive Blood Cultures:

    • Positive blood cultures are detected through appearance, subculture plates, or instrument-based detection.
    • Gram-stained smears and other tests are performed to identify and test antimicrobial susceptibility.

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    Bloodstream Infections Chapter 67 PDF

    Description

    Test your knowledge on blood culture methods and techniques with this informative quiz. Explore topics such as media composition, incubation conditions, and specialized techniques for detecting microorganisms. Learn how to handle positive blood cultures and identify the organisms present. Perfect for medical professionals and laboratory technicians.

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