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Questions and Answers
Which type of organism primarily infects immunosuppressed patients and those with serious or terminal illness?
Which type of organism primarily infects immunosuppressed patients and those with serious or terminal illness?
Which type of organism is particularly found in neonates receiving lipid-supplemented parenteral nutrition?
Which type of organism is particularly found in neonates receiving lipid-supplemented parenteral nutrition?
Which type of organism can disseminate to all body organs?
Which type of organism can disseminate to all body organs?
Which type of organism enters the blood via invasion of lymph nodes and other organs?
Which type of organism enters the blood via invasion of lymph nodes and other organs?
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Which type of organism primarily infects the target organ or cells?
Which type of organism primarily infects the target organ or cells?
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Which type of organism infects the endocardium and is most commonly caused by bacteria?
Which type of organism infects the endocardium and is most commonly caused by bacteria?
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Which agar is used to culture Gram-negative bacteria?
Which agar is used to culture Gram-negative bacteria?
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Which organisms are considered probable contaminants in blood cultures?
Which organisms are considered probable contaminants in blood cultures?
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Which condition is NOT consistent with sepsis based on clinical presentation?
Which condition is NOT consistent with sepsis based on clinical presentation?
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Which microorganisms may require alternative methods for diagnosis if they cause bloodstream infections?
Which microorganisms may require alternative methods for diagnosis if they cause bloodstream infections?
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What is the incubation period for Brucella spp. in blood cultures?
What is the incubation period for Brucella spp. in blood cultures?
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When should the physician be notified of positive culture results?
When should the physician be notified of positive culture results?
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Which factor is NOT a contributing factor to bloodstream infections?
Which factor is NOT a contributing factor to bloodstream infections?
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What is the recommended volume of blood to collect for blood cultures in adults?
What is the recommended volume of blood to collect for blood cultures in adults?
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What is the purpose of using anticoagulants in blood culture collection?
What is the purpose of using anticoagulants in blood culture collection?
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What is the recommended concentration of sodium polyanethol sulfonate (SPS) as an anticoagulant?
What is the recommended concentration of sodium polyanethol sulfonate (SPS) as an anticoagulant?
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What is the recommended number of blood cultures to obtain for optimum sensitivity in patients with endocarditis who have not received antibiotics?
What is the recommended number of blood cultures to obtain for optimum sensitivity in patients with endocarditis who have not received antibiotics?
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What should phlebotomists do to prevent contamination during blood drawing?
What should phlebotomists do to prevent contamination during blood drawing?
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Which of the following is NOT a component of blood culture media?
Which of the following is NOT a component of blood culture media?
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What is the recommended blood to medium ratio in traditional dilution blood factor?
What is the recommended blood to medium ratio in traditional dilution blood factor?
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What is the purpose of venting the bottles in conventional blood cultures?
What is the purpose of venting the bottles in conventional blood cultures?
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Which method combines liquid and solid medium in the same bottle for easy subculture?
Which method combines liquid and solid medium in the same bottle for easy subculture?
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What is the purpose of instrument-based systems like BACTEC and BacT/ALERT?
What is the purpose of instrument-based systems like BACTEC and BacT/ALERT?
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How are positive blood cultures detected?
How are positive blood cultures detected?
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Which term refers to the presence of bacteria in the bloodstream?
Which term refers to the presence of bacteria in the bloodstream?
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What are the clinical manifestations of septicemia?
What are the clinical manifestations of septicemia?
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Which bacteria are most commonly isolated in bloodstream infections?
Which bacteria are most commonly isolated in bloodstream infections?
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What is the purpose of using anticoagulants in blood cultures?
What is the purpose of using anticoagulants in blood cultures?
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How are probable pathogens distinguished from contaminants in a blood culture?
How are probable pathogens distinguished from contaminants in a blood culture?
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Which term refers to the presence of bacteria in the blood?
Which term refers to the presence of bacteria in the blood?
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What is the suffix 'emia' derived from?
What is the suffix 'emia' derived from?
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Which of the following is a clinical manifestation of septicemia?
Which of the following is a clinical manifestation of septicemia?
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Which bacteria is most commonly isolated in bloodstream infections?
Which bacteria is most commonly isolated in bloodstream infections?
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What is the process of specimen collection, handling, processing, and culturing for bacteremia detection called?
What is the process of specimen collection, handling, processing, and culturing for bacteremia detection called?
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What are the major types of bloodstream infections?
What are the major types of bloodstream infections?
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Which organisms may require alternative methods for diagnosis if they cause bloodstream infections?
Which organisms may require alternative methods for diagnosis if they cause bloodstream infections?
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What is the incubation period for Brucella spp. in blood cultures?
What is the incubation period for Brucella spp. in blood cultures?
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Which agar is used to culture Gram-negative bacteria?
Which agar is used to culture Gram-negative bacteria?
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What is the purpose of venting the bottles in conventional blood cultures?
What is the purpose of venting the bottles in conventional blood cultures?
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Which type of organism infects the endocardium and is most commonly caused by bacteria?
Which type of organism infects the endocardium and is most commonly caused by bacteria?
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What is the recommended number of blood cultures to obtain for optimum sensitivity in patients with endocarditis who have not received antibiotics?
What is the recommended number of blood cultures to obtain for optimum sensitivity in patients with endocarditis who have not received antibiotics?
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Which type of organism primarily infects immunosuppressed patients and those with serious or terminal illness?
Which type of organism primarily infects immunosuppressed patients and those with serious or terminal illness?
Signup and view all the answers
What is the recommended volume of blood to collect for blood cultures in adults?
What is the recommended volume of blood to collect for blood cultures in adults?
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What is the purpose of using anticoagulants in blood culture collection?
What is the purpose of using anticoagulants in blood culture collection?
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Which factor is NOT a contributing factor to bloodstream infections?
Which factor is NOT a contributing factor to bloodstream infections?
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Which of the following is a clinical manifestation of septicemia?
Which of the following is a clinical manifestation of septicemia?
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What is the purpose of using anticoagulants in blood culture collection?
What is the purpose of using anticoagulants in blood culture collection?
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Which type of organism primarily infects immunosuppressed patients and those with serious or terminal illness?
Which type of organism primarily infects immunosuppressed patients and those with serious or terminal illness?
Signup and view all the answers
What is the recommended number of blood cultures to obtain for optimum sensitivity in patients with endocarditis who have not received antibiotics?
What is the recommended number of blood cultures to obtain for optimum sensitivity in patients with endocarditis who have not received antibiotics?
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Which term refers to the presence of bacteria in the bloodstream?
Which term refers to the presence of bacteria in the bloodstream?
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Which type of organism primarily infects immunosuppressed patients and those with serious or terminal illness?
Which type of organism primarily infects immunosuppressed patients and those with serious or terminal illness?
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Which type of organism enters the blood via invasion of lymph nodes and other organs?
Which type of organism enters the blood via invasion of lymph nodes and other organs?
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What is the most common route of entry for bacteremia from an extravascular site?
What is the most common route of entry for bacteremia from an extravascular site?
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Which type of organism is particularly found in neonates receiving lipid-supplemented parenteral nutrition?
Which type of organism is particularly found in neonates receiving lipid-supplemented parenteral nutrition?
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Which type of organism primarily infects the endocardium and is most commonly caused by bacteria?
Which type of organism primarily infects the endocardium and is most commonly caused by bacteria?
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What is the recommended number of blood cultures to obtain for optimum sensitivity in patients with infective endocarditis who have not received antibiotics?
What is the recommended number of blood cultures to obtain for optimum sensitivity in patients with infective endocarditis who have not received antibiotics?
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What is the recommended volume of blood to collect for blood cultures in infants and children?
What is the recommended volume of blood to collect for blood cultures in infants and children?
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What is the most commonly used anticoagulant in blood culture collection?
What is the most commonly used anticoagulant in blood culture collection?
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What is the purpose of venting the bottles in conventional blood cultures?
What is the purpose of venting the bottles in conventional blood cultures?
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Which factor is NOT a contributing factor to bloodstream infections?
Which factor is NOT a contributing factor to bloodstream infections?
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Which of the following is a special culture/culturing condition for Brucella spp. in blood cultures?
Which of the following is a special culture/culturing condition for Brucella spp. in blood cultures?
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Which of the following is NOT a criterion to distinguish probable pathogens from contaminants in blood cultures?
Which of the following is NOT a criterion to distinguish probable pathogens from contaminants in blood cultures?
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Which of the following organisms is known as 'nutritionally variant' or 'satelliting' streptococci?
Which of the following organisms is known as 'nutritionally variant' or 'satelliting' streptococci?
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Which agar is used to culture Campylobacter in blood cultures?
Which agar is used to culture Campylobacter in blood cultures?
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Which of the following organisms is NOT considered a probable contaminant in blood cultures?
Which of the following organisms is NOT considered a probable contaminant in blood cultures?
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Which method combines liquid and solid medium in the same bottle for easy subculture?
Which method combines liquid and solid medium in the same bottle for easy subculture?
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What is the purpose of using anticoagulants in blood culture collection?
What is the purpose of using anticoagulants in blood culture collection?
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Which condition is NOT consistent with sepsis based on clinical presentation?
Which condition is NOT consistent with sepsis based on clinical presentation?
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What is the recommended concentration of sodium polyanethol sulfonate (SPS) as an anticoagulant?
What is the recommended concentration of sodium polyanethol sulfonate (SPS) as an anticoagulant?
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Which agar is used to culture Gram-negative bacteria?
Which agar is used to culture Gram-negative bacteria?
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Study Notes
Blood Culture Methods and Techniques
- Gelatin can counteract the inhibitory action of SPS, but it may decrease the recovery of other organisms.
- Dilution blood factor: traditionally a 1:10 ratio of blood to medium, but new commercial media with additives may use a 1:5 ratio.
- Blood culture media typically contain nutrient broth, anticoagulant, and various additives.
- Some specialized media can absorb and inactivate antimicrobial agents, increasing the yield of microorganisms.
- Incubation conditions include temperature and time, with longer incubation required for certain microorganisms.
- Conventional blood cultures use commercially prepared bottles with low oxidation-reduction potential.
- Venting the bottles may be necessary for growth of obligate aerobes.
- Constant agitation during the first 24 hours enhances the growth of most aerobic bacteria.
- Bottles are examined daily for growth indicators like turbidity, gas, and discoloration.
- The biphasic method combines liquid and solid medium in the same bottle, allowing for easy subculture.
- Lysis centrifugation is a method used to detect microorganisms in body fluid specimens.
- Instrument-based systems like BACTEC and BacT/ALERT measure the production of CO2 by metabolizing microorganisms.
Handling Positive Blood Cultures:
- Positive blood cultures are detected through appearance, subculture plates, or instrument-based detection.
- Gram-stained smears and other tests are performed to identify and test antimicrobial susceptibility.
Blood Culture Methods and Techniques
- Gelatin can counteract the inhibitory action of SPS, but it may decrease the recovery of other organisms.
- Dilution blood factor: traditionally a 1:10 ratio of blood to medium, but new commercial media with additives may use a 1:5 ratio.
- Blood culture media typically contain nutrient broth, anticoagulant, and various additives.
- Some specialized media can absorb and inactivate antimicrobial agents, increasing the yield of microorganisms.
- Incubation conditions include temperature and time, with longer incubation required for certain microorganisms.
- Conventional blood cultures use commercially prepared bottles with low oxidation-reduction potential.
- Venting the bottles may be necessary for growth of obligate aerobes.
- Constant agitation during the first 24 hours enhances the growth of most aerobic bacteria.
- Bottles are examined daily for growth indicators like turbidity, gas, and discoloration.
- The biphasic method combines liquid and solid medium in the same bottle, allowing for easy subculture.
- Lysis centrifugation is a method used to detect microorganisms in body fluid specimens.
- Instrument-based systems like BACTEC and BacT/ALERT measure the production of CO2 by metabolizing microorganisms.
Handling Positive Blood Cultures:
- Positive blood cultures are detected through appearance, subculture plates, or instrument-based detection.
- Gram-stained smears and other tests are performed to identify and test antimicrobial susceptibility.
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Description
Test your knowledge on blood culture methods and techniques with this informative quiz. Explore topics such as media composition, incubation conditions, and specialized techniques for detecting microorganisms. Learn how to handle positive blood cultures and identify the organisms present. Perfect for medical professionals and laboratory technicians.
