Medical Microbiology Procedures

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Questions and Answers

Which antiseptic is recommended before collecting a blood culture?

  • Chlorhexidine
  • Iodine
  • Alcohol followed by iodine (correct)
  • Alcohol

What is the main purpose of using an anticoagulant in blood cultures?

  • Neutralizing bacteriocidal effect of human serum (correct)
  • Maintaining the condition for anaerobic bacteria growth
  • Preventing phagocytosis
  • Enhancing antibiotic removal capability

Which bacterial strain is associated with Oral Thrush?

  • Bordetella pertussis
  • Candida albicans (correct)
  • Neisseria gonorrhoeae
  • Haemophilus influenzae type B

In what type of specimen is an anaerobic culture appropriate for Lower Respiratory Tract Culture?

<p>Sputum collected by deep cough (B)</p> Signup and view all the answers

For which infection is a rectal swab the recommended specimen for culture?

<p>Gonorrhea (D)</p> Signup and view all the answers

Which preservative maintains the appropriate colony counts for urine specimens at room temperature?

<p>Boric Acid (C)</p> Signup and view all the answers

What is the purpose of using charcoal in specimen preservation?

<p>To absorb fatty acids inhibiting the growth of fastidious bacteria (D)</p> Signup and view all the answers

Which type of specimen is stored in an incubator at 35°C?

<p>CSF (A)</p> Signup and view all the answers

What does direct microscopy primarily assist in?

<p>Detection and characterization of microorganisms (D)</p> Signup and view all the answers

In which temperature range are genital specimens and corneal swabs stored?

<p>22 to 25°C (D)</p> Signup and view all the answers

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Study Notes

Blood Culture and Antiseptics

  • 2% chlorhexidine is the recommended antiseptic before collecting a blood culture.

Blood Culture and Anticoagulants

  • The main purpose of using an anticoagulant in blood cultures is to prevent clotting.

Bacterial Strains and Infections

  • Candida albicans is the bacterial strain associated with Oral Thrush.

Lower Respiratory Tract Culture

  • An anaerobic culture is appropriate for bronchoalveolar lavage (BAL) or pleural fluid specimens.

Infections and Specimen Collection

  • A rectal swab is the recommended specimen for culture in Neisseria gonorrhoeae infections.

Urine Specimen Preservation

  • Boric acid is the preservative that maintains the appropriate colony counts for urine specimens at room temperature.

Specimen Preservation

  • Charcoal is used in specimen preservation to absorb and remove inhibitors.

Specimen Storage

  • Blood culture specimens are stored in an incubator at 35°C.

Direct Microscopy

  • Direct microscopy primarily assists in the rapid identification of pathogens.

Genital and Corneal Specimen Storage

  • Genital specimens and corneal swabs are stored at a temperature range of 2-8°C.

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