18 Questions
Which type of broth favors the growth of anaerobes?
Thioglycolate
What is the primary purpose of a biphasic blood culture system?
To provide a solid agar phase for inoculation with broth medium
What is the term for the process of sampling blood for microbial growth?
Venipuncture
Which medium is a general-purpose medium that facilitates the recovery of yeasts and Gram-positive organisms?
Brain-Heart Infusion
What is the term for the presence of bacteria in the bloodstream?
Bacteremia
What is the purpose of a blind subculture?
To perform a subculture in the absence of visual signs of growth
What is the primary limitation of using charcoal in blood culture bottles?
It creates a haziness in the broth, compromising visual detection of growth
What is the purpose of using a biphasic bottle in blood culture?
To facilitate growth detection in low concentration of bacteria in blood
Why are repeated subcultures necessary for detecting growth in blood culture?
Due to the presence of debris and hemolysis of dying red blood cells
What is the advantage of using seethrough plastic or glass in blood culture bottles?
It allows for visual detection of growth
What is the limitation of the blood culture system described by Castañeda?
It is no longer commercially available
Why is a large volume of blood necessary for blood culture in cases with low concentration of bacteria?
To facilitate growth detection
What is a crucial condition for defining a bloodstream infection episode?
Recovery of a pathogen different from the initial pathogen ≥48 h after the recovery of the initial pathogen
What is the purpose of a hypertonic medium like Brucella broth in blood culture?
To improve cellular stability and increase recovery rates of certain bacteria
What is the primary function of sodium-polyanethole sulfate (SPS) in blood culture?
To inhibit lysozyme and inactivate certain antibiotics
What is the difference between a manual blood culture bottle and an automated blood culture system?
Manual systems do not use automated equipment, whereas automated systems do
What is the purpose of a subculture in blood culture?
To make a secondary culture of bacteria from another culture
What is the main difference between a terminal subculture and a supplemented peptone broth?
Supplemented peptone broth is superior to terminal subculture for most common pathogens
This quiz assesses your understanding of blood culture detection in low and middle-income countries, including the importance of visual detection and the design of blood culture bottles to optimize growth visualization. Learn about the challenges and limitations of using certain materials and additives in blood culture media.
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