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Blood Culture Detection in Low and Middle-Income Countries

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Questions and Answers

Which type of broth favors the growth of anaerobes?

Thioglycolate (correct)
Tryptic Soy broth
Brain-Heart Infusion
Thiol broth

What is the primary purpose of a biphasic blood culture system?

To provide a solid agar phase for inoculation with broth medium (correct)
To detect bloodstream infections more efficiently
To incubate and monitor blood culture bottles
To facilitate the growth of Pseudomonas species

What is the term for the process of sampling blood for microbial growth?

Blind subculture
Venipuncture (correct)
Bloodstream infection
Blood culture

Which medium is a general-purpose medium that facilitates the recovery of yeasts and Gram-positive organisms?

Brain-Heart Infusion (A) Signup and view all the answers

What is the term for the presence of bacteria in the bloodstream?

Bacteremia (B) Signup and view all the answers

What is the purpose of a blind subculture?

To perform a subculture in the absence of visual signs of growth (B) Signup and view all the answers

What is the primary limitation of using charcoal in blood culture bottles?

It creates a haziness in the broth, compromising visual detection of growth (D) Signup and view all the answers

What is the purpose of using a biphasic bottle in blood culture?

To facilitate growth detection in low concentration of bacteria in blood (A) Signup and view all the answers

Why are repeated subcultures necessary for detecting growth in blood culture?

Due to the presence of debris and hemolysis of dying red blood cells (C) Signup and view all the answers

What is the advantage of using seethrough plastic or glass in blood culture bottles?

It allows for visual detection of growth (A) Signup and view all the answers

What is the limitation of the blood culture system described by Castañeda?

It is no longer commercially available (A) Signup and view all the answers

Why is a large volume of blood necessary for blood culture in cases with low concentration of bacteria?

To facilitate growth detection (D) Signup and view all the answers

What is a crucial condition for defining a bloodstream infection episode?

Recovery of a pathogen different from the initial pathogen ≥48 h after the recovery of the initial pathogen (D) Signup and view all the answers

What is the purpose of a hypertonic medium like Brucella broth in blood culture?

To improve cellular stability and increase recovery rates of certain bacteria (D) Signup and view all the answers

What is the primary function of sodium-polyanethole sulfate (SPS) in blood culture?

To inhibit lysozyme and inactivate certain antibiotics (A) Signup and view all the answers

What is the difference between a manual blood culture bottle and an automated blood culture system?

Manual systems do not use automated equipment, whereas automated systems do (C) Signup and view all the answers

What is the purpose of a subculture in blood culture?

To make a secondary culture of bacteria from another culture (C) Signup and view all the answers

What is the main difference between a terminal subculture and a supplemented peptone broth?

Supplemented peptone broth is superior to terminal subculture for most common pathogens (B) Signup and view all the answers

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