Blood Collection Tubes and Additives

Sodium polyanethol sulfonate (SPS) is an additive used for ______________ specimen.

Acid citrate dextrose (ACD) is an additive used for ______________ studies.

Lithium heparin is an additive used in tubes with a ______________ separator.

ROYAL BLUE TOP tubes are used for ______________ testing.

PINK TOP tubes are used for ______________.

PEACH TOP tubes are used for ______________ testing.

Most tubes have an ______ - ANTICOAGULANT to prevent clotting of blood sample

The color of the stopper on each tube indicates what, if any, ______ the tube contains

The amount of ______ in a tube has been calibrated by the manufacturer

Tubes sealed with a partial vacuum inside by rubber stoppers. Vacuum is artificially created by pulling air from the tube. The amount of vacuum is measured precisely by the manufacturer so that the tube will draw the exact volume of ______ indicated on the label

Different blood tests require different types of blood specimens. Most tubes have ______ - ANTICOAGULANT to prevent clotting of blood sample

The amount of ______ in a tube has been calibrated by the manufacturer. Most tubes have an additive - ANTICOAGULANT to prevent clotting of blood sample

RED TOP a.b.______ No additive Clot activator Test Routine blood chemistry Serology tests Blood bank procedure z EVACUATED TUBES & ADDITIVES 2.GOLD TOP a.b.______ Gel separator Clot activator Test Routine blood chemistry Diagnostic testing of serum for infectious diseases HLA typing z EVACUATED TUBES & ADDITIVES 3.LIGHT BLUE TOP a.______ b.Sodium citrate – removes calcium Test Prothrombin time APTT D-dimer Fibrinogen z z EVACUATED TUBES & ADDITIVES 4.GREEN TOP a.______ b.Heparin – inhibits thrombin formation Test Arterial blood gas Therapeutic drug monitoring Electrolyte analysis z EVACUATED TUBES & ADDITIVES 5.PURPLE/LAVENDER TOP a.______ b.Ethylene diamine tetraacetic acid (EDTA) – removes calcium Test Complete blood count Erythrocyte sedimentation rate Peripheral blood smear Reticulocyte count HbA1C z EVACUATED TUBES & ADDITIVES 6.BLACK TOP a.______ b.Buffered sodium citrate Test Erythrocyte sedimentation rate BLACK TOP LIGHT BLUE TOP 3.8% concentration 3.2% concentration 0.129 M 0.109 M 1:4 (anticoagulant:blood) 1:9 (anticoagulant:blood) ESR, original Westergren method Coagulation studies z EVACUATED TUBES & ADDITIVES 7.GREY TOP a.b.______ Potassium oxalate – precipitates calcium Sodium fluoride – inhibits glycolysis Test Glucose determination Gamma-glutamyl transferase (GGT) Blood alcohol level z EVACUATED TUBES & ADDITIVES 8.BROWN/TAN TOP a.______ b.

RED TOP a.b.______ No ______ Clot activator Test Routine blood chemistry Serology tests Blood bank procedure z EVACUATED TUBES & ADDITIVES 2.GOLD TOP a.b.______ Gel separator Clot activator Test Routine blood chemistry Diagnostic testing of serum for infectious diseases HLA typing z EVACUATED TUBES & ADDITIVES

GREEN TOP a.______ b.Heparin – inhibits thrombin formation Test Arterial blood gas Therapeutic drug monitoring Electrolyte analysis z EVACUATED TUBES & ADDITIVES

PURPLE/LAVENDER TOP a.______ b.Ethylene diamine tetraacetic acid (EDTA) – removes calcium Test Complete blood count Erythrocyte sedimentation rate Peripheral blood smear Reticulocyte count HbA1C z EVACUATED TUBES & ADDITIVES

BLACK TOP a.______ b.Buffered sodium citrate Test Erythrocyte sedimentation rate BLACK TOP LIGHT BLUE TOP 3.8% concentration 3.2% concentration 0.129 M 0.109 M 1:4 (anticoagulant:blood) 1:9 (anticoagulant:blood) ESR, original Westergren method Coagulation studies z EVACUATED TUBES & ADDITIVES

GREY TOP a.b.______ Potassium oxalate – precipitates calcium Sodium fluoride – inhibits glycolysis Test Glucose determination Gamma-glutamyl transferase (GGT) Blood alcohol level z EVACUATED TUBES & ADDITIVES