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Questions and Answers
Sodium polyanethol sulfonate (SPS) is an additive used for ______________ specimen.
Sodium polyanethol sulfonate (SPS) is an additive used for ______________ specimen.
blood culture
Acid citrate dextrose (ACD) is an additive used for ______________ studies.
Acid citrate dextrose (ACD) is an additive used for ______________ studies.
blood bank
Lithium heparin is an additive used in tubes with a ______________ separator.
Lithium heparin is an additive used in tubes with a ______________ separator.
gel
ROYAL BLUE TOP tubes are used for ______________ testing.
ROYAL BLUE TOP tubes are used for ______________ testing.
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PINK TOP tubes are used for ______________.
PINK TOP tubes are used for ______________.
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PEACH TOP tubes are used for ______________ testing.
PEACH TOP tubes are used for ______________ testing.
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Most tubes have an ______ - ANTICOAGULANT to prevent clotting of blood sample
Most tubes have an ______ - ANTICOAGULANT to prevent clotting of blood sample
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The color of the stopper on each tube indicates what, if any, ______ the tube contains
The color of the stopper on each tube indicates what, if any, ______ the tube contains
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The amount of ______ in a tube has been calibrated by the manufacturer
The amount of ______ in a tube has been calibrated by the manufacturer
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Tubes sealed with a partial vacuum inside by rubber stoppers. Vacuum is artificially created by pulling air from the tube. The amount of vacuum is measured precisely by the manufacturer so that the tube will draw the exact volume of ______ indicated on the label
Tubes sealed with a partial vacuum inside by rubber stoppers. Vacuum is artificially created by pulling air from the tube. The amount of vacuum is measured precisely by the manufacturer so that the tube will draw the exact volume of ______ indicated on the label
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Different blood tests require different types of blood specimens. Most tubes have ______ - ANTICOAGULANT to prevent clotting of blood sample
Different blood tests require different types of blood specimens. Most tubes have ______ - ANTICOAGULANT to prevent clotting of blood sample
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The amount of ______ in a tube has been calibrated by the manufacturer. Most tubes have an additive - ANTICOAGULANT to prevent clotting of blood sample
The amount of ______ in a tube has been calibrated by the manufacturer. Most tubes have an additive - ANTICOAGULANT to prevent clotting of blood sample
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RED TOP a.b.______ No additive Clot activator Test Routine blood chemistry Serology tests Blood bank procedure z EVACUATED TUBES & ADDITIVES 2.GOLD TOP a.b.______ Gel separator Clot activator Test Routine blood chemistry Diagnostic testing of serum for infectious diseases HLA typing z EVACUATED TUBES & ADDITIVES 3.LIGHT BLUE TOP a.______ b.Sodium citrate – removes calcium Test Prothrombin time APTT D-dimer Fibrinogen z z EVACUATED TUBES & ADDITIVES 4.GREEN TOP a.______ b.Heparin – inhibits thrombin formation Test Arterial blood gas Therapeutic drug monitoring Electrolyte analysis z EVACUATED TUBES & ADDITIVES 5.PURPLE/LAVENDER TOP a.______ b.Ethylene diamine tetraacetic acid (EDTA) – removes calcium Test Complete blood count Erythrocyte sedimentation rate Peripheral blood smear Reticulocyte count HbA1C z EVACUATED TUBES & ADDITIVES 6.BLACK TOP a.______ b.Buffered sodium citrate Test Erythrocyte sedimentation rate BLACK TOP LIGHT BLUE TOP 3.8% concentration 3.2% concentration 0.129 M 0.109 M 1:4 (anticoagulant:blood) 1:9 (anticoagulant:blood) ESR, original Westergren method Coagulation studies z EVACUATED TUBES & ADDITIVES 7.GREY TOP a.b.______ Potassium oxalate – precipitates calcium Sodium fluoride – inhibits glycolysis Test Glucose determination Gamma-glutamyl transferase (GGT) Blood alcohol level z EVACUATED TUBES & ADDITIVES 8.BROWN/TAN TOP a.______ b.
RED TOP a.b.______ No additive Clot activator Test Routine blood chemistry Serology tests Blood bank procedure z EVACUATED TUBES & ADDITIVES 2.GOLD TOP a.b.______ Gel separator Clot activator Test Routine blood chemistry Diagnostic testing of serum for infectious diseases HLA typing z EVACUATED TUBES & ADDITIVES 3.LIGHT BLUE TOP a.______ b.Sodium citrate – removes calcium Test Prothrombin time APTT D-dimer Fibrinogen z z EVACUATED TUBES & ADDITIVES 4.GREEN TOP a.______ b.Heparin – inhibits thrombin formation Test Arterial blood gas Therapeutic drug monitoring Electrolyte analysis z EVACUATED TUBES & ADDITIVES 5.PURPLE/LAVENDER TOP a.______ b.Ethylene diamine tetraacetic acid (EDTA) – removes calcium Test Complete blood count Erythrocyte sedimentation rate Peripheral blood smear Reticulocyte count HbA1C z EVACUATED TUBES & ADDITIVES 6.BLACK TOP a.______ b.Buffered sodium citrate Test Erythrocyte sedimentation rate BLACK TOP LIGHT BLUE TOP 3.8% concentration 3.2% concentration 0.129 M 0.109 M 1:4 (anticoagulant:blood) 1:9 (anticoagulant:blood) ESR, original Westergren method Coagulation studies z EVACUATED TUBES & ADDITIVES 7.GREY TOP a.b.______ Potassium oxalate – precipitates calcium Sodium fluoride – inhibits glycolysis Test Glucose determination Gamma-glutamyl transferase (GGT) Blood alcohol level z EVACUATED TUBES & ADDITIVES 8.BROWN/TAN TOP a.______ b.
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RED TOP a.b.______ No ______ Clot activator Test Routine blood chemistry Serology tests Blood bank procedure z EVACUATED TUBES & ADDITIVES 2.GOLD TOP a.b.______ Gel separator Clot activator Test Routine blood chemistry Diagnostic testing of serum for infectious diseases HLA typing z EVACUATED TUBES & ADDITIVES
RED TOP a.b.______ No ______ Clot activator Test Routine blood chemistry Serology tests Blood bank procedure z EVACUATED TUBES & ADDITIVES 2.GOLD TOP a.b.______ Gel separator Clot activator Test Routine blood chemistry Diagnostic testing of serum for infectious diseases HLA typing z EVACUATED TUBES & ADDITIVES
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GREEN TOP a.______ b.Heparin – inhibits thrombin formation Test Arterial blood gas Therapeutic drug monitoring Electrolyte analysis z EVACUATED TUBES & ADDITIVES
GREEN TOP a.______ b.Heparin – inhibits thrombin formation Test Arterial blood gas Therapeutic drug monitoring Electrolyte analysis z EVACUATED TUBES & ADDITIVES
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PURPLE/LAVENDER TOP a.______ b.Ethylene diamine tetraacetic acid (EDTA) – removes calcium Test Complete blood count Erythrocyte sedimentation rate Peripheral blood smear Reticulocyte count HbA1C z EVACUATED TUBES & ADDITIVES
PURPLE/LAVENDER TOP a.______ b.Ethylene diamine tetraacetic acid (EDTA) – removes calcium Test Complete blood count Erythrocyte sedimentation rate Peripheral blood smear Reticulocyte count HbA1C z EVACUATED TUBES & ADDITIVES
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BLACK TOP a.______ b.Buffered sodium citrate Test Erythrocyte sedimentation rate BLACK TOP LIGHT BLUE TOP 3.8% concentration 3.2% concentration 0.129 M 0.109 M 1:4 (anticoagulant:blood) 1:9 (anticoagulant:blood) ESR, original Westergren method Coagulation studies z EVACUATED TUBES & ADDITIVES
BLACK TOP a.______ b.Buffered sodium citrate Test Erythrocyte sedimentation rate BLACK TOP LIGHT BLUE TOP 3.8% concentration 3.2% concentration 0.129 M 0.109 M 1:4 (anticoagulant:blood) 1:9 (anticoagulant:blood) ESR, original Westergren method Coagulation studies z EVACUATED TUBES & ADDITIVES
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GREY TOP a.b.______ Potassium oxalate – precipitates calcium Sodium fluoride – inhibits glycolysis Test Glucose determination Gamma-glutamyl transferase (GGT) Blood alcohol level z EVACUATED TUBES & ADDITIVES
GREY TOP a.b.______ Potassium oxalate – precipitates calcium Sodium fluoride – inhibits glycolysis Test Glucose determination Gamma-glutamyl transferase (GGT) Blood alcohol level z EVACUATED TUBES & ADDITIVES
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Study Notes
Blood Collection Tubes and Additives
- Sodium polyanethol sulfonate (SPS) is an additive for blood culture specimens.
- Acid citrate dextrose (ACD) is used for various blood studies, often in blood banking.
- Lithium heparin is present in tubes that contain a gel separator for serum analysis.
- ROYAL BLUE TOP tubes are designated for trace element testing.
- PINK TOP tubes are employed for blood compatibility testing.
- PEACH TOP tubes are suitable for specific molecular testing.
- Most blood collection tubes contain an anticoagulant to prevent clotting.
- The color of tube stoppers indicates the type of additive each tube contains.
- The volume of blood required in each tube is calibrated by the manufacturer.
Vacuum System in Tubes
- Tubes are sealed with rubber stoppers, creating a partial vacuum.
- The vacuum is generated by removing air to allow precise blood draw.
- Each tube is designed to draw a specific volume of blood indicated on the label.
Types of Evacuated Tubes and Their Uses
- RED TOP: No additive; used for routine blood chemistry, serology tests, and blood bank procedures.
- GOLD TOP: Contains gel separator and clot activator; used for routine blood chemistry and diagnostic testing.
- LIGHT BLUE TOP: Contains sodium citrate; used for prothrombin time, APTT, D-dimer, and fibrinogen tests.
- GREEN TOP: Contains heparin; used for arterial blood gas, therapeutic drug monitoring, and electrolyte analysis.
- PURPLE/LAVENDER TOP: Contains EDTA; used for complete blood counts, erythrocyte sedimentation rates, and HbA1C testing.
- BLACK TOP: Contains buffered sodium citrate; used specifically for erythrocyte sedimentation rates.
- GREY TOP: Contains potassium oxalate and sodium fluoride; used for glucose determination, GGT, and blood alcohol levels.
- BROWN/TAN TOP: Typically indicates no additive; similar uses as red top.
Anticoagulant Concentrations
- Light blue top contains buffered sodium citrate at specific concentrations of 3.8% and 3.2%.
- The anticoagulant to blood ratio in certain tests is critical, e.g., 1:4 for black top and 1:9 for light blue top tubes.
Understanding the purpose and properties of each blood collection tube is essential for accurate laboratory testing and diagnostics.
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Description
Test your knowledge on different types of blood collection tubes and their additives used for various tests such as routine blood chemistry, serology tests, and blood bank procedures.
