Blood Collection Tubes and Anticoagulants

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Questions and Answers

What is the primary use of a tube containing EDTA?

  • Clotting time tests
  • Glucose testing
  • von Willebrand Factor Antigen measurement
  • Hematology tests (correct)

If a blood sample is collected in a green-topped tube, which type of test is most likely to be performed?

  • Electrolyte panel
  • Complete blood count
  • Coagulation studies (correct)
  • Glucose level assessment

For accurate glucose testing with an Oxalate Fluoride tube, what sample type is acceptable?

  • Whole blood or Plasma (correct)
  • Whole blood only
  • Plasma only
  • Serum only

What is the required concentration of EDTA in a blood sample?

<p>1.5 mg per mL of blood (C)</p> Signup and view all the answers

What is a critical concern regarding the volume of blood in tubes used for coagulation studies?

<p>Underfilling or overfilling can affect results (B)</p> Signup and view all the answers

What color is the tube commonly used for general biochemistry tests like bile acids?

<p>Brown/Clear (B)</p> Signup and view all the answers

Which anticoagulant is present in tubes that are typically lavender or red?

<p>EDTA (D)</p> Signup and view all the answers

A test measuring Prothrombin Time(PT) is ordered. What color should the tube be?

<p>Green (A)</p> Signup and view all the answers

For a glucose test, which anticoagulant is used, and what are the possible tube colors?

<p>Oxalate Fluoride; Yellow or Grey (B)</p> Signup and view all the answers

Which tube type is used to test for the von Willebrand Factor Antigen?

<p>Green (B)</p> Signup and view all the answers

What is the required concentration of EDTA per milliliter of blood?

<p>1.5 mg/ml (C)</p> Signup and view all the answers

Which of these tubes contains an anti-coagulant?

<p>Orange tube (C)</p> Signup and view all the answers

Which of the following is a measurement of blood clotting time?

<p>APTT (B)</p> Signup and view all the answers

If a biochemistry test for enzymes is required, which tube should be used?

<p>Orange tube (C)</p> Signup and view all the answers

Why is it important to fill test tubes to the specified fill line?

<p>To ensure accuracy of results (A)</p> Signup and view all the answers

Flashcards

Lavender/Red Tube

This tube contains EDTA, an anticoagulant used for hematology tests. It prevents blood clotting by binding to calcium.

Green Tube

These tubes contain sodium citrate, an anticoagulant used in clotting studies. It prevents blood clotting by binding to calcium.

Yellow or Grey Tube

This tube contains oxalate fluoride, an anticoagulant and antiglycolytic agent. It prevents blood clotting and glucose breakdown.

EDTA Anticoagulant

This tube contains EDTA, an anticoagulant used for hematology tests. It prevents blood clotting by binding to calcium.

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Capillary Blood Sample

A small amount of blood collected from the fingertip.

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Plain Tubes (BROWN/CLEAR)

Tubes without any additives, used for general biochemistry tests like serum analysis and hormone testing.

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Heparin Tubes (ORANGE)

Tubes containing heparin, a natural anticoagulant. Used for biochemistry tests related to enzymes and electrolytes.

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EDTA Tubes (LAVENDER/RED)

Tubes containing EDTA, a synthetic anticoagulant. Primarily used for blood cell analysis.

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Fluoride Oxalate Tubes (YELLOW/GREY)

Tubes containing sodium fluoride and oxalate, preventing clotting and preserving glucose. Used for blood glucose testing.

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Sodium Citrate Tubes (GREEN)

Tubes containing sodium citrate, used for blood clotting tests like PT and PTT.

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Prothrombin Time (PT)

A test that measures the time it takes for blood to clot, a major indicator of clotting disorders.

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Activated Partial Thromboplastin Time (APTT)

A test that measures the time for blood to clot in the presence of an activator. Used for evaluating intrinsic coagulation pathway.

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von Willebrand Factor Antigen

A protein found in blood that helps platelets stick together and form a blood clot. Used for understanding blood clotting problems.

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Filling Tubes to Line

The importance of filling tubes to a specific line ensures accurate blood sample results.

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Thorough Mixing

Mixing the blood sample thoroughly after collection is crucial for consistent test results.

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Study Notes

أنابيب سحب الدم ومضادات التجلط

  • أنابيب سحب الدم لونها يحدد نوع مضاد التجلط المضاف لها.
  • الأنابيب البنية أو الشفافة (BROWN/CLEAR) تُستخدم للفحوصات الكيميائية العامة (General Biochemistry) مثل أحماض الصفراء، علم الأمصال، وعلم الغدد الصماء.
  • أنابيب برتقالية (ORANGE) تحتوي على هيبارين، مستخدمة لفحوصات بيوكيميائية، بما في ذلك الإنزيمات و البوتاسيوم. يُستخدم تركيز 10-20 وحدة دولية لكل مل من الدم.
  • أنابيب أرجوانية أو حمراء (LAVENDER/RED) تحتوي على EDTA، تُستخدم لفحوصات أمراض الدم (Hematology). التركيز المطلوب 1.5 ملغ لكل مل من الدم.
  • أنابيب صفراء أو رمادية (YELLOW/GREY) تحتوي على أكسالات الفلوريد، تُستخدم لفحوصات الجلوكوز، والعينة عادةً دم كامل أو بلازما.
  • أنابيب خضراء (GREEN) تحتوي على سيترات الصوديوم، تُستخدم لفحوصات تخثر الدم (Clotting Times) مثل PT و APTT وقياس عامل فون ويلبراند. التركيز 1 مل لكل 9 مل من الدم أو 1 مل لكل 4 مل من الدم.
  • يجب ملء الأنابيب حتى خط الامتلاء المحدد
  • يجب خلط العينة جيدًا بعد السحب
  • حجم العينة غير الصحيح يمكن أن يؤثر على نتائج الاختبار بشكل ملحوظ، خاصةً اختبارات تخثر الدم.

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