Blood Cell Counting Procedure Quiz
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Questions and Answers

What is the purpose of adding WBCs diluting fluid to the blood sample?

  • To increase the number of WBCs in the sample
  • To make a dilution of 20 times (correct)
  • To mix the blood with EDTA solution
  • To decrease the viscosity of the blood sample

    • What is the next step after filling the Neubauer chamber with diluted blood?

  • Shaking the chamber vigorously
  • Staining the blood cells with a dye
  • Covering the chamber with a lid
  • Ensuring there are no air bubbles in the chamber (correct)

    • Why is it important for the cells to be uniformly distributed over the ruled area of the chamber?

  • To prevent cell clumping (correct)
  • To avoid contamination of the sample
  • To speed up the settling time
  • To make counting easier

    • How many small squares should be counted in each corner square when tallying WBCs?

    <p>16 small squares</p> Signup and view all the answers

    What do WBCs look like when viewed under the microscope in the Neubauer chamber?

    <p>Black dots</p> Signup and view all the answers

    What formula is used to calculate the total WBC count per mm3?

    <p>$Total WBCs count / mm^3 = No$</p> Signup and view all the answers

    What is the formula for calculating the multiply factor for WBCs?

    <p>Multiply factor = dilution factor / volume of fluid inside chamber</p> Signup and view all the answers

    What is the volume of fluid inside the chamber in mm³?

    <p>0.4 mm³</p> Signup and view all the answers

    If the sum of cells counted in the four corner areas is 200, what would be the total WBC count per liter?

    <p>5 × 10^9 cells/L</p> Signup and view all the answers

    What would be the total WBC count per liter if 250 cells were observed in the corner squares?

    <p>25 × 10^9 cells/L</p> Signup and view all the answers

    What is the total WBC count per liter if 180 cells are counted in the corner squares?

    <p>10 × 10^9 cells/L</p> Signup and view all the answers

    What is the normal range for white blood cell (WBC) count per unit volume of whole blood?

    <p>4000-11000 cell/μL</p> Signup and view all the answers

    What medical condition is indicated by a high white blood cell count (leukocytosis)?

    <p>Rheumatoid arthritis</p> Signup and view all the answers

    What does a low white blood cell count (leukopenia) indicate?

    <p>Bone marrow disorders</p> Signup and view all the answers

    What is the composition of Turk's solution used in the WBC count test?

    <p>Glacial acetic acid, gention violet, water</p> Signup and view all the answers

    What type of cells are white blood cells in circulation, based on the text?

    <p>Translucent cells</p> Signup and view all the answers

    Which condition is NOT associated with a high white blood cell count (leukocytosis)?

    <p>Iron deficiency anemia</p> Signup and view all the answers

    What is the formula to calculate the total WBC count per liter?

    <p>Total WBC count per liter = No. of WBCs x multiply factor</p> Signup and view all the answers

    How is the volume of fluid inside the chamber calculated?

    <p>Volume = Area x Depth</p> Signup and view all the answers

    If 180 cells are counted in the corner squares, what would be the total WBC count per liter?

    <p>Total WBC count per liter = 180 cells x 50 = 9000 cells/L</p> Signup and view all the answers

    What is the depth of the chamber in mm?

    <p>0.1 mm</p> Signup and view all the answers

    How is the multiply factor for WBCs calculated?

    <p>Multiply factor = dilution factor / volume of fluid inside chamber</p> Signup and view all the answers

    What is the purpose of placing a coverslip in position over the ruled area of the chamber?

    <p>To ensure that the distribution of cells is uniform and to prevent air bubbles from forming.</p> Signup and view all the answers

    How should WBCs be counted in the Neubauer chamber using the microscope?

    <p>By using the 10 X magnification and focusing on the 4 corner squares.</p> Signup and view all the answers

    What do WBCs look like when viewed under the microscope in the Neubauer chamber?

    <p>WBCs appear as black dots.</p> Signup and view all the answers

    Why is it important to count only the WBCs that fall on the squares' left and top borders?

    <p>To avoid double counting and ensure accurate results.</p> Signup and view all the answers

    What is the calculation formula for the total WBC count per mm³?

    <p>Total WBCs count per mm³ = No.</p> Signup and view all the answers

    What is the total WBC count per mm³ if 150 cells are counted in the Neubauer chamber?

    <p>150 WBCs per mm³</p> Signup and view all the answers

    What is the medical term for a high white blood cell count?

    <p>leukocytosis</p> Signup and view all the answers

    Name a condition that can result in a low white blood cell count.

    <p>Primary bone marrow disorders</p> Signup and view all the answers

    What is the purpose of Turk's solution in the WBC count test?

    <p>To destroy red cells and stain the nuclei of leukocytes</p> Signup and view all the answers

    What is the normal range for white blood cell count per unit volume of whole blood?

    <p>4000-11000 cells/μL</p> Signup and view all the answers

    What does a low white blood cell count indicate?

    <p>Leukopenia</p> Signup and view all the answers

    Name a condition that can cause leukocytosis.

    <p>Inflammation</p> Signup and view all the answers

    Study Notes

    WBC Count Test

    • WBCs diluting fluid is added to the blood sample to prevent clotting and enable accurate counting.
    • After filling the Neubauer chamber with diluted blood, the next step is to place a coverslip in position over the ruled area of the chamber.

    Neubauer Chamber

    • It is essential for the cells to be uniformly distributed over the ruled area of the chamber to ensure accurate counting.
    • When tallying WBCs, 4-5 small squares in each corner square should be counted.

    WBC Characteristics

    • WBCs appear as clear or blue-gray cells with a nucleus when viewed under the microscope in the Neubauer chamber.

    WBC Count Calculation

    • The formula to calculate the total WBC count per mm³ is: (Total cells counted x 400) / (Number of squares counted x Depth of chamber in mm)
    • The formula for calculating the multiply factor for WBCs is: 400 / (Number of squares counted x Depth of chamber in mm)

    Neubauer Chamber Specifications

    • The volume of fluid inside the chamber is 0.0004 mm³ (or 0.4 mm³ / 1000).
    • The depth of the chamber is 0.1 mm.

    WBC Count Interpretation

    • The normal range for white blood cell (WBC) count per unit volume of whole blood is 4,000-11,000 cells per mm³.
    • A high white blood cell count (leukocytosis) indicates infection, inflammation, or tissue damage.
    • A low white blood cell count (leukopenia) indicates a weakened immune system or bone marrow disorder.

    Turk's Solution

    • Turk's solution, composed of acetic acid and gentian violet, is used to lyse red blood cells and stain WBCs in the WBC count test.

    WBCs in Circulation

    • White blood cells in circulation are a type of leukocyte.

    Medical Conditions

    • Leukocytosis is associated with infections, inflammation, or tissue damage, but not with pregnancy.
    • Leukopenia can result from cancer treatment, aplastic anemia, or viral infections.

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    Related Documents

    Total WBC Count Test PDF

    Description

    Test your knowledge on the procedure for counting blood cells using a Neubauer slide and Turk's solution. Questions cover preparation steps and proper application techniques for accurate results.

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