Blood Cell Counting Procedure Quiz

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Questions and Answers

What is the purpose of adding WBCs diluting fluid to the blood sample?

To increase the number of WBCs in the sample

To make a dilution of 20 times (correct)

To mix the blood with EDTA solution

To decrease the viscosity of the blood sample

What is the next step after filling the Neubauer chamber with diluted blood?

Shaking the chamber vigorously

Staining the blood cells with a dye

Covering the chamber with a lid

Ensuring there are no air bubbles in the chamber (correct)

Why is it important for the cells to be uniformly distributed over the ruled area of the chamber?

To prevent cell clumping (correct)

To avoid contamination of the sample

To speed up the settling time

To make counting easier

How many small squares should be counted in each corner square when tallying WBCs?

16 small squares Signup and view all the answers

What do WBCs look like when viewed under the microscope in the Neubauer chamber?

Black dots Signup and view all the answers

What formula is used to calculate the total WBC count per mm3?

$Total WBCs count / mm^3 = No$ Signup and view all the answers

What is the formula for calculating the multiply factor for WBCs?

Multiply factor = dilution factor / volume of fluid inside chamber Signup and view all the answers

What is the volume of fluid inside the chamber in mm³?

0.4 mm³ Signup and view all the answers

If the sum of cells counted in the four corner areas is 200, what would be the total WBC count per liter?

5 × 10^9 cells/L Signup and view all the answers

What would be the total WBC count per liter if 250 cells were observed in the corner squares?

25 × 10^9 cells/L Signup and view all the answers

What is the total WBC count per liter if 180 cells are counted in the corner squares?

10 × 10^9 cells/L Signup and view all the answers

What is the normal range for white blood cell (WBC) count per unit volume of whole blood?

4000-11000 cell/μL Signup and view all the answers

What medical condition is indicated by a high white blood cell count (leukocytosis)?

Rheumatoid arthritis Signup and view all the answers

What does a low white blood cell count (leukopenia) indicate?

Bone marrow disorders Signup and view all the answers

What is the composition of Turk's solution used in the WBC count test?

Glacial acetic acid, gention violet, water Signup and view all the answers

What type of cells are white blood cells in circulation, based on the text?

Translucent cells Signup and view all the answers

Which condition is NOT associated with a high white blood cell count (leukocytosis)?

Iron deficiency anemia Signup and view all the answers

What is the formula to calculate the total WBC count per liter?

Total WBC count per liter = No. of WBCs x multiply factor Signup and view all the answers

How is the volume of fluid inside the chamber calculated?

Volume = Area x Depth Signup and view all the answers

If 180 cells are counted in the corner squares, what would be the total WBC count per liter?

Total WBC count per liter = 180 cells x 50 = 9000 cells/L Signup and view all the answers

What is the depth of the chamber in mm?

0.1 mm Signup and view all the answers

How is the multiply factor for WBCs calculated?

Multiply factor = dilution factor / volume of fluid inside chamber Signup and view all the answers

What is the purpose of placing a coverslip in position over the ruled area of the chamber?

To ensure that the distribution of cells is uniform and to prevent air bubbles from forming. Signup and view all the answers

How should WBCs be counted in the Neubauer chamber using the microscope?

By using the 10 X magnification and focusing on the 4 corner squares. Signup and view all the answers

What do WBCs look like when viewed under the microscope in the Neubauer chamber?

WBCs appear as black dots. Signup and view all the answers

Why is it important to count only the WBCs that fall on the squares' left and top borders?

To avoid double counting and ensure accurate results. Signup and view all the answers

What is the calculation formula for the total WBC count per mm³?

Total WBCs count per mm³ = No. Signup and view all the answers

What is the total WBC count per mm³ if 150 cells are counted in the Neubauer chamber?

150 WBCs per mm³ Signup and view all the answers

What is the medical term for a high white blood cell count?

leukocytosis Signup and view all the answers

Name a condition that can result in a low white blood cell count.

Primary bone marrow disorders Signup and view all the answers

What is the purpose of Turk's solution in the WBC count test?

To destroy red cells and stain the nuclei of leukocytes Signup and view all the answers

What is the normal range for white blood cell count per unit volume of whole blood?

4000-11000 cells/μL Signup and view all the answers

What does a low white blood cell count indicate?

Leukopenia Signup and view all the answers

Name a condition that can cause leukocytosis.

Inflammation Signup and view all the answers

Study Notes

WBC Count Test

WBCs diluting fluid is added to the blood sample to prevent clotting and enable accurate counting.
After filling the Neubauer chamber with diluted blood, the next step is to place a coverslip in position over the ruled area of the chamber.

Neubauer Chamber

It is essential for the cells to be uniformly distributed over the ruled area of the chamber to ensure accurate counting.
When tallying WBCs, 4-5 small squares in each corner square should be counted.

WBC Characteristics

WBCs appear as clear or blue-gray cells with a nucleus when viewed under the microscope in the Neubauer chamber.

WBC Count Calculation

The formula to calculate the total WBC count per mm³ is: (Total cells counted x 400) / (Number of squares counted x Depth of chamber in mm)
The formula for calculating the multiply factor for WBCs is: 400 / (Number of squares counted x Depth of chamber in mm)

Neubauer Chamber Specifications

The volume of fluid inside the chamber is 0.0004 mm³ (or 0.4 mm³ / 1000).
The depth of the chamber is 0.1 mm.

WBC Count Interpretation

The normal range for white blood cell (WBC) count per unit volume of whole blood is 4,000-11,000 cells per mm³.
A high white blood cell count (leukocytosis) indicates infection, inflammation, or tissue damage.
A low white blood cell count (leukopenia) indicates a weakened immune system or bone marrow disorder.

Turk's Solution

Turk's solution, composed of acetic acid and gentian violet, is used to lyse red blood cells and stain WBCs in the WBC count test.

WBCs in Circulation

White blood cells in circulation are a type of leukocyte.

Medical Conditions

Leukocytosis is associated with infections, inflammation, or tissue damage, but not with pregnancy.
Leukopenia can result from cancer treatment, aplastic anemia, or viral infections.

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Test your knowledge on the procedure for counting blood cells using a Neubauer slide and Turk's solution. Questions cover preparation steps and proper application techniques for accurate results.

Blood Cell Counting Procedure Quiz

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Questions and Answers

What is the purpose of adding WBCs diluting fluid to the blood sample?

What is the next step after filling the Neubauer chamber with diluted blood?

Why is it important for the cells to be uniformly distributed over the ruled area of the chamber?

How many small squares should be counted in each corner square when tallying WBCs?

What do WBCs look like when viewed under the microscope in the Neubauer chamber?

What formula is used to calculate the total WBC count per mm3?

What is the formula for calculating the multiply factor for WBCs?

What is the volume of fluid inside the chamber in mm³?

If the sum of cells counted in the four corner areas is 200, what would be the total WBC count per liter?

What would be the total WBC count per liter if 250 cells were observed in the corner squares?

What is the total WBC count per liter if 180 cells are counted in the corner squares?

What is the normal range for white blood cell (WBC) count per unit volume of whole blood?

What medical condition is indicated by a high white blood cell count (leukocytosis)?

What does a low white blood cell count (leukopenia) indicate?

What is the composition of Turk's solution used in the WBC count test?

What type of cells are white blood cells in circulation, based on the text?

Which condition is NOT associated with a high white blood cell count (leukocytosis)?

What is the formula to calculate the total WBC count per liter?

How is the volume of fluid inside the chamber calculated?

If 180 cells are counted in the corner squares, what would be the total WBC count per liter?

What is the depth of the chamber in mm?

How is the multiply factor for WBCs calculated?

What is the purpose of placing a coverslip in position over the ruled area of the chamber?

How should WBCs be counted in the Neubauer chamber using the microscope?

What do WBCs look like when viewed under the microscope in the Neubauer chamber?

Why is it important to count only the WBCs that fall on the squares' left and top borders?

What is the calculation formula for the total WBC count per mm³?

What is the total WBC count per mm³ if 150 cells are counted in the Neubauer chamber?

What is the medical term for a high white blood cell count?

Name a condition that can result in a low white blood cell count.

What is the purpose of Turk's solution in the WBC count test?

What is the normal range for white blood cell count per unit volume of whole blood?

What does a low white blood cell count indicate?

Name a condition that can cause leukocytosis.

Study Notes

WBC Count Test

Neubauer Chamber

WBC Characteristics

WBC Count Calculation

Neubauer Chamber Specifications

WBC Count Interpretation

Turk's Solution

WBCs in Circulation

Medical Conditions

Studying That Suits You

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