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Questions and Answers
What is the principle behind cell counting and sizing in the Electrical Impedance (Coulter principle) method?
What type of blood specimen is required for cell counting using the Electrical Impedance method?
What is the function of the lysing reagent in the context of cell counting using the Electrical Impedance method?
What is the purpose of the electrolyte in the context of cell counting using the Electrical Impedance method?
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What is the role of the cleaning agents in the Electrical Impedance method of cell counting?
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Study Notes
Electrical Impedance (Coulter Principle)
- The principle behind cell counting and sizing is based on changes in electrical impedance (resistance to alternating current) when a cell passes through a narrow tube.
Sample Requirements
- The Electrical Impedance method requires a whole blood specimen.
Lysing Reagent
- The lysing reagent is used to dissolve red blood cells and platelets, leaving only white blood cells intact for counting.
Electrolyte
- The electrolyte is necessary to facilitate the flow of electrical current and enable detection of cells as they pass through the tube.
Cleaning Agents
- Cleaning agents are used to prevent clogging of the tube and ensure accurate counting by removing any residual cells and debris.
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Description
Test your knowledge of the principles of blood cell counting with this quiz. Explore the classic methods of Electrical Impedance (Coulter principle) and Optical detection/scatter used in the process.