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Blood Cell Counting Principles Quiz

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Questions and Answers

What is the principle behind cell counting and sizing in the Electrical Impedance (Coulter principle) method?

Detection and measurement of changes in electrical resistance produced by cells as they traverse a small aperture (correct)

Detection and measurement of changes in magnetic field induced by cells in a closed chamber

Detection and measurement of light scatter by cells passing through a detection system

Detection and measurement of changes in temperature caused by cells as they pass through a sensor

What type of blood specimen is required for cell counting using the Electrical Impedance method?

Arterial blood in EDTA, undiluted capillary blood

Whole venous blood in heparinized capillary blood

Whole venous blood in EDTA, diluted/heparinized capillary blood (correct)

Arterial blood in heparinized capillary blood

What is the function of the lysing reagent in the context of cell counting using the Electrical Impedance method?

Stain the cells for better visibility under the microscope

Neutralize the electrical charge of the cells

Prevent clotting of the blood specimen

Dissolve RBC stroma when performing WBC counts (correct)

What is the purpose of the electrolyte in the context of cell counting using the Electrical Impedance method?

<p>Used to dilute cells and flush the instrument</p> Signup and view all the answers

What is the role of the cleaning agents in the Electrical Impedance method of cell counting?

<p>Remove protein buildup from aperture tube and electrodes</p> Signup and view all the answers

Study Notes

Electrical Impedance (Coulter Principle)

The principle behind cell counting and sizing is based on changes in electrical impedance (resistance to alternating current) when a cell passes through a narrow tube.

Sample Requirements

The Electrical Impedance method requires a whole blood specimen.

Lysing Reagent

The lysing reagent is used to dissolve red blood cells and platelets, leaving only white blood cells intact for counting.

Electrolyte

The electrolyte is necessary to facilitate the flow of electrical current and enable detection of cells as they pass through the tube.

Cleaning Agents

Cleaning agents are used to prevent clogging of the tube and ensure accurate counting by removing any residual cells and debris.

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Description

Test your knowledge of the principles of blood cell counting with this quiz. Explore the classic methods of Electrical Impedance (Coulter principle) and Optical detection/scatter used in the process.