Podcast
Questions and Answers
What is the primary goal of using enzymes in red cell antibody testing?
What is the primary goal of using enzymes in red cell antibody testing?
- To detect strong antibodies
- To differentiate between antibody mixtures
- To enhance the reaction of weak antibodies (correct)
- To abolish the reaction of enzyme-resistant antigens
Which of the following antibodies is not enhanced by enzymes?
Which of the following antibodies is not enhanced by enzymes?
- P1
- Kidd
- Duffy (correct)
- Rh
What happens to MNSs antibodies when treated with enzymes?
What happens to MNSs antibodies when treated with enzymes?
- They are denatured (correct)
- They remain unchanged
- They are enhanced
- They are abolished
Which enzyme is commonly used in Blood Bank?
Which enzyme is commonly used in Blood Bank?
What is the effect of enzymes on Xga antibodies?
What is the effect of enzymes on Xga antibodies?
What is the typical temperature at which autoantibodies react with RBCs in Warm AIHA?
What is the typical temperature at which autoantibodies react with RBCs in Warm AIHA?
What is the primary site of RBC destruction in Warm AIHA?
What is the primary site of RBC destruction in Warm AIHA?
What is NOT a clinical feature of Warm AIHA?
What is NOT a clinical feature of Warm AIHA?
What is a common treatment for Warm AIHA?
What is a common treatment for Warm AIHA?
What is the distinguishing feature of Cold AIHA?
What is the distinguishing feature of Cold AIHA?
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Study Notes
Enzyme Method in Blood Banking
- Bromelin, Papain, Ficin, and Tripsin are common enzymes used in Blood Banking.
- The enzyme method has two primary goals in red cell antibody testing:
Goals of Enzyme Method
- To detect weak antibodies by increasing the strength of reaction (enhance).
- To differentiate between antibodies in an antibody mixture by abolishing the reaction of antibody against enzyme-sensitive antigen.
- Enzymes can enhance the detection of certain antibodies, including:
Enhanced Antibodies
- Rh antibodies.
- Kidd antibodies.
- Lewis antibodies.
- P1 antibodies.
- Conversely, enzymes can denature certain antibodies, including:
Denatured Antibodies
- Duffy antibodies.
- MNSs antibodies.
- Xga antibodies.
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