Biosafety Principles in Anatomy and Histology
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Questions and Answers

What is the definition of biosafety?

Biosafety is the application of safety precautions that reduce a laboratorian's risk of exposure to potentially infectious material and limit contamination of the work environment and ultimately the community.

Name three biohazardous materials mentioned in the text.

Viruses, Bacteria, Fungi

What are the major components of a biosafety program?

1- Good lab work practice - Laboratory practice and techniques 2- Safety equipments and facilities - Laminar air flow cabinet 3- Personal protective equipments - Glove, eye and mouth mask

What is the purpose of Biosafety Level 1 (BSL1)?

<p>BSL1 involves working with agents not known to cause disease and poses minimal hazard to humans and the environment.</p> Signup and view all the answers

What is the purpose of Biosafety Level 2 (BSL2)?

<p>BSL2 involves working with agents of moderate hazard to personnel and minimal hazard to the environment, including various bacteria and viruses that cause only mild disease to humans.</p> Signup and view all the answers

What are the safety practices followed in Biosafety Level 1 (BSL1)?

<p>Standard microbiological practices are followed, work can be performed on open bench tops, and personal protective equipment is needed.</p> Signup and view all the answers

What are the key practices for Biosafety Level 4 (BSL4)?

<p>Change clothes before entering, shower upon exiting, decontaminate all materials before exiting, and use a separate building for the lab.</p> Signup and view all the answers

Name two Risk Group 3 agents mentioned in the text.

<p>Mycobacterium tuberculosis and Leishmania donovani</p> Signup and view all the answers

What is the purpose of a biological safety cabinet (BSC)?

<p>To provide a ventilated and enclosed workspace for safely working with contaminated materials.</p> Signup and view all the answers

What is the significance of a self-closing door with automatic locking in Biosafety Level 3 (BSL3) practices?

<p>It helps to maintain a secure and controlled environment within the laboratory.</p> Signup and view all the answers

Name two examples of Risk Group 2 agents mentioned in the text.

<p>Hepatitis A and Herpes Simplex Virus</p> Signup and view all the answers

What is the purpose of the sink with eyewash in BSL3 practices?

<p>To provide immediate flushing of the eyes in case of accidental exposure to hazardous materials.</p> Signup and view all the answers

What are the principles of biosafety?

<p>To protect the patient, yourself, and the environment.</p> Signup and view all the answers

What are the major components of a biosafety program?

<p>1- Good lab work practice - Laboratory practice and techniques, 2- Safety equipments and facilities - Laminar air flow cabinet, 3- Personal protective equipments - Glove, eye and mouth mask</p> Signup and view all the answers

What are the biohazardous materials mentioned in the text?

<p>Viruses, Bacteria, Fungi, Prions</p> Signup and view all the answers

What is the purpose of a biological safety cabinet (BSC)?

<p>To provide a safe work environment when handling biohazardous materials.</p> Signup and view all the answers

What are the Biosafety Level 1 (BSL1) practices?

<p>Standard microbiological practices are followed. Work can be performed on open bench tops. Personal protective equipment is needed. Sink for hand washing.</p> Signup and view all the answers

What is the purpose of the sink with hand washing in BSL3 practices?

<p>To maintain hygiene and minimize the risk of contamination during the handling of hazardous materials.</p> Signup and view all the answers

What agents are involved in Biosafety Level 2 (BSL2)?

<p>Agents of moderate hazard to personnel and minimal to the environment, including various bacteria and viruses that cause only mild disease to humans.</p> Signup and view all the answers

What is the purpose of the sink with eyewash in BSL3 practices?

<p>To provide immediate irrigation in case of eye exposure to hazardous materials.</p> Signup and view all the answers

What are the key differences between Biosafety Level 3 (BSL3) and Biosafety Level 4 (BSL4) practices?

<p>BSL3 works with dangerous and exotic agents that pose a high individual and moderate environmental risk, while BSL4 works with lethal, pathogenic agents that pose a high individual and environmental risk with no effective treatment available.</p> Signup and view all the answers

What is the purpose of the exhaust air not being recirculated in BSL3 practices?

<p>The purpose is to prevent the recirculation of potentially contaminated air, thus reducing the risk of exposure to dangerous agents.</p> Signup and view all the answers

Why is it necessary to have a separate building for BSL4 lab?

<p>Having a separate building helps to contain and isolate the work with lethal, pathogenic agents, reducing the risk of exposure to these agents outside the lab.</p> Signup and view all the answers

What are the key characteristics of a Class III biological safety cabinet (BSC)?

<p>A Class III BSC is a totally enclosed, gas-tight cabinet with glove ports for working with hazardous materials, providing maximum protection for the user, environment, and the product being handled.</p> Signup and view all the answers

Why is it important to decontaminate all materials before exiting a BSL4 lab?

<p>Decontamination of all materials is crucial to prevent the accidental release of lethal, pathogenic agents outside the lab, thereby maintaining a secure environment.</p> Signup and view all the answers

What is the significance of the self-closing doors with automatic locking in BSL2 practices?

<p>The self-closing doors with automatic locking help prevent unauthorized access and inadvertent exposure to the agents being worked with, thus enhancing the overall safety and security of the lab.</p> Signup and view all the answers

Why are laboratories under medical surveillance and receive immunization in BSL3 practices?

<p>This is to ensure the health and safety of personnel working with dangerous and exotic agents that pose a high individual risk, as well as to prevent the potential spread of these agents to the surrounding environment.</p> Signup and view all the answers

What is the purpose of the sink with eyewash in BSL3 practices?

<p>The sink with eyewash is a safety measure to quickly flush the eyes with water in case of accidental exposure to hazardous agents, minimizing the risk of eye injury or contamination.</p> Signup and view all the answers

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