Biosafety Levels (BSL) PDF
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Uploaded by ahmedsafaa
University of Al-Ameed College of Medicine
Zainab R. Radhi
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Summary
This document provides a detailed overview of biosafety levels and procedures in a laboratory setting. It covers various aspects of laboratory design, types of pathogens, and safety precautions, and discusses the importance of handling potentially infectious materials safely.
Full Transcript
University of Al-Ameed College of Medicine Department of anatomy and histology Practical Biology Lecture 1 Biosafety MSC. Zainab R. Radhi Definition Biosafety is the application of safety precautions that reduce a laboratorians risk of exposure to potentially infectious material and limit cont...
University of Al-Ameed College of Medicine Department of anatomy and histology Practical Biology Lecture 1 Biosafety MSC. Zainab R. Radhi Definition Biosafety is the application of safety precautions that reduce a laboratorians risk of exposure to potentially infectious material and limit contamination of the work environment and ultimately the community. Principles of biosafety To protect: • the patient • yourself • the environment Loading… Biohazardous Materials • • • • Viruses Bacteria Fungi Prions Major components of Biosafety program 1- Good lab work practice - Laboratory practice and techniques 2- Safety equipments and facilities - Laminar air flow cabinet 3- Personal protective equipments - Glove, eye and mouth mask Loading… Laboratory Design Biosafety Levels (BSL) o BSL1 o BSL2 o BSL3 o BSL4 Biosafety Level-1 o Involve working with agents not known to cause disease o Minimal hazard to human and the Risk Group 1 Agents environment • • • • E.coli Fungi Mold Yeast BSL - 1 Practices • Standard microbiological practices are followed. • Work can be performed on an open bench tops. • Personal protective equipment needed. • Sink - hand washing. Biosafety Level-2 • Involving agents of moderate hazard to personnel and minimal to environment • Includes various bacteria and viruses that cause only mild disease to humans . Loading… Risk Group 2 Agents • • • Hepatitis A, B Herpes Simplex Virus Influenza A BSL – 2 Practices • Access to lab is restricted when work is being conducted. • Personal protective equipment • Biosafety cabinet. • Autoclave / decontamination proper. • Self closing doors. Biosafety Level-3 o Work with dangerous and exotic agents that pose a high individual and moderate environmental risk Risk Group 3 Agents o Mycobacterium tuberculosis o Leishmania donovani o SARS Leishmania donovani BSL – 3 Practices • Laboratories under medical surveillance and receive immunization. • Personal protective equipment. • Sink with eyewash. • Exhaust air – not recirculated. • Self closing doors with automatic locking Biosafety Level 4 – BSL4 • work with lethal, pathogenic agent that pose a high individual and environmental risk. • Effective treatment not Risk Group 4available. Agents usually • Lassa Fever Virus • Ebola Hemorrhagic Fever Virus • Herpes B Virus BSL –4 Practices • Change clothes before entering. • Shower upon exiting. • Decontaminate all materials before exiting. • Separate building for lab. Biological safety cabinet (BSC) It is an enclosed, ventilated laboratory workspace for safely working with materials contaminated. BSC Type :: o Class I o Class II o Class III ANY QUESTIONS?
