NCERT - BIOMOLECULES - 24-25
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Questions and Answers

What color does starch-I2 turn when iodine is present?

  • Yellow
  • Green
  • Red
  • Blue (correct)

Cellulose can hold iodine molecules similar to starch.

False (B)

What is the main component of plant cell walls?

Cellulose

The complex polysaccharide found in arthropod exoskeletons is known as ______.

<p>chitin</p> Signup and view all the answers

Which of the following is NOT a nitrogenous base found in nucleic acids?

<p>Glucose (C)</p> Signup and view all the answers

Match the nucleic acids with their sugar component:

<p>DNA = Ribose RNA = Deoxyribose</p> Signup and view all the answers

A nucleotide consists of a heterocyclic compound, a monosaccharide, and a phosphoric acid.

<p>True (A)</p> Signup and view all the answers

What are the two types of sugars found in nucleic acids?

<p>Ribose and deoxyribose</p> Signup and view all the answers

Which of the following is an example of an aromatic amino acid?

<p>Phenylalanine (A)</p> Signup and view all the answers

All amino acids are soluble in water.

<p>False (B)</p> Signup and view all the answers

What are the two functional groups present in amino acids?

<p>Amino group and carboxyl group</p> Signup and view all the answers

Fatty acids can be classified as either _____ or _____ based on the presence of double bonds.

<p>saturated, unsaturated</p> Signup and view all the answers

Match the following fatty acids with their carbon count:

<p>Palmitic acid = 16 carbons Arachidonic acid = 20 carbons Stearic acid = 18 carbons Oleic acid = 18 carbons</p> Signup and view all the answers

Which of the following is NOT considered a proteinaceous amino acid?

<p>Palmitic acid (C)</p> Signup and view all the answers

What are phospholipids primarily found in?

<p>Cell membranes</p> Signup and view all the answers

Triglycerides are formed by the esterification of fatty acids with glycerol.

<p>True (A)</p> Signup and view all the answers

What describes the tertiary structure of a protein?

<p>A three-dimensional folding of the protein chain (C)</p> Signup and view all the answers

The quaternary structure of proteins involves a single folded polypeptide chain.

<p>False (B)</p> Signup and view all the answers

How many subunits are present in adult human haemoglobin?

<p>4</p> Signup and view all the answers

An enzyme's _____ site is where the substrate fits and reactions are catalyzed.

<p>active</p> Signup and view all the answers

Match the types of protein structure with their descriptions:

<p>Primary Structure = A sequence of amino acids Secondary Structure = Local folds such as alpha helices and beta sheets Tertiary Structure = Three-dimensional folding of a single polypeptide Quaternary Structure = Arrangement of multiple polypeptide chains</p> Signup and view all the answers

What is indicated by 'active site' in an enzyme?

<p>The area where the enzyme catalyzes a reaction (B)</p> Signup and view all the answers

Almost all enzymes are proteins.

<p>True (A)</p> Signup and view all the answers

What structural feature is formed when individual polypeptide chains come together?

<p>Quaternary structure</p> Signup and view all the answers

Which of the following is considered a primary metabolite?

<p>Amino acids (C)</p> Signup and view all the answers

Secondary metabolites have identifiable functions and roles in normal physiological processes.

<p>False (B)</p> Signup and view all the answers

Name one example of a secondary metabolite.

<p>Morphine</p> Signup and view all the answers

The molecular weight of compounds in the acid soluble pool ranges from _____ to around 800 daltons.

<p>18</p> Signup and view all the answers

Match the following examples with their respective categories of metabolites:

<p>Morphine = Alkaloids Rubber = Polymeric substances Vinblastin = Drugs Lemon grass oil = Essential oils</p> Signup and view all the answers

What is a common characteristic of biomolecules found in the acid insoluble fraction?

<p>They include proteins and nucleic acids. (C)</p> Signup and view all the answers

Toxins, such as Ricin, are classified as secondary metabolites.

<p>True (A)</p> Signup and view all the answers

What is the molecular weight range for lipids?

<p>Ten thousand daltons and above</p> Signup and view all the answers

What is the term for the process when enzyme activity is shut off by a chemical?

<p>Inhibition (A)</p> Signup and view all the answers

Competitive inhibitors do not resemble the substrate in molecular structure.

<p>False (B)</p> Signup and view all the answers

Name the class of enzymes that catalyze the transfer of groups other than hydrogen between substrates.

<p>Transferases</p> Signup and view all the answers

The enzyme succinic dehydrogenase is inhibited by a competitive inhibitor known as ______.

<p>malonate</p> Signup and view all the answers

Match the following classes of enzymes with their functions:

<p>Oxidoreductases = Catalyze oxidation-reduction reactions Transferases = Catalyze transfer of groups between substrates Hydrolases = Catalyze hydrolysis of bonds Lyases = Catalyze removal of groups from substrates leaving double bonds</p> Signup and view all the answers

Which of the following types of reactions are catalyzed by hydrolases?

<p>Hydrolysis of bonds (C)</p> Signup and view all the answers

Isomerases are responsible for the removal of groups from substrates.

<p>False (B)</p> Signup and view all the answers

How many classes of enzymes are there, based on the text?

<p>Six</p> Signup and view all the answers

What is the energy difference between the stable substrate (S) and the product (P) called when it is higher than the transition state?

<p>Activation energy (D)</p> Signup and view all the answers

An exothermic reaction requires energy from outside sources to form the product.

<p>False (B)</p> Signup and view all the answers

What term describes the short-lived complex formed when an enzyme binds with a substrate?

<p>Enzyme-substrate complex</p> Signup and view all the answers

The higher energy state that substrates must go through during a reaction is called the ______.

<p>transition state</p> Signup and view all the answers

Which of the following correctly describes the role of enzymes in a chemical reaction?

<p>They lower activation energy barriers. (A)</p> Signup and view all the answers

Match the following terms with their correct descriptions:

<p>Substrate (S) = The reactant molecule that binds to an enzyme Product (P) = The molecule that results from the enzymatic reaction Activation energy = The energy required to reach the transition state Enzyme-substrate complex (ES) = The transient complex formed during enzyme catalysis</p> Signup and view all the answers

All intermediate structural states between a stable substrate and product are stable.

<p>False (B)</p> Signup and view all the answers

What happens to the enzyme after it catalyzes a reaction and forms the product?

<p>It remains unchanged.</p> Signup and view all the answers

Which elements are present in both living tissues and the earth's crust?

<p>Carbon, Hydrogen and Oxygen (A)</p> Signup and view all the answers

The relative abundance of carbon and hydrogen in living organisms is lower than in the earth's crust.

<p>False (B)</p> Signup and view all the answers

What are the two fractions obtained after grinding living tissue in trichloroacetic acid?

<p>Filtrate and Retentate</p> Signup and view all the answers

The process used to discover organic compounds from living tissues involves performing a chemical ______.

<p>analysis</p> Signup and view all the answers

Match the following elements with their percentage weight in the human body:

<p>Hydrogen = 0.5 Carbon = 18.5 Oxygen = 65 Nitrogen = 3</p> Signup and view all the answers

What is the primary component of the filtrate obtained from the chemical analysis of living tissues?

<p>Organic compounds (D)</p> Signup and view all the answers

Acid-soluble pool contains thousands of organic compounds.

<p>True (A)</p> Signup and view all the answers

What technique is used to separate compounds from the acid-soluble pool?

<p>Separation techniques</p> Signup and view all the answers

What structure is formed when multiple polypeptide chains come together?

<p>Quaternary structure (D)</p> Signup and view all the answers

Which of the following amino acids is classified as basic?

<p>Lysine (B)</p> Signup and view all the answers

Tertiary structure is important for the biological activities of proteins.

<p>True (A)</p> Signup and view all the answers

How many subunits make up adult human haemoglobin?

<p>4</p> Signup and view all the answers

All amino acids have a variable group designated as an R group.

<p>True (A)</p> Signup and view all the answers

An enzyme's active site is a ______ into which the substrate fits.

<p>pocket</p> Signup and view all the answers

Name one example of a saturated fatty acid.

<p>Palmitic acid</p> Signup and view all the answers

The zwitterionic form refers to the ______ structure of amino acids in different pH solutions.

<p>ionizable</p> Signup and view all the answers

Match the following protein structures with their descriptions:

<p>Primary structure = Amino acid sequence of the protein Secondary structure = Local folding into helices or sheets Tertiary structure = Three-dimensional folding of a single polypeptide Quaternary structure = Assembly of multiple polypeptide chains</p> Signup and view all the answers

Match the following amino acids with their type based on R group characteristics:

<p>Glycine = Neutral amino acid Alanine = Neutral amino acid Lysine = Basic amino acid Glutamic acid = Acidic amino acid</p> Signup and view all the answers

Which of the following describes the tertiary structure of a protein?

<p>The overall three-dimensional shape of a single polypeptide (C)</p> Signup and view all the answers

Almost all enzymes are ______.

<p>proteins</p> Signup and view all the answers

Which of the following statements is true about lipids?

<p>Phospholipids contain phosphorus. (A)</p> Signup and view all the answers

Ribozymes are proteins that act as enzymes.

<p>False (B)</p> Signup and view all the answers

Fatty acids can only have one carbon chain.

<p>False (B)</p> Signup and view all the answers

What is the primary distinguishing feature of phospholipids?

<p>They contain phosphorus.</p> Signup and view all the answers

What are the two main categories of biomolecules based on their molecular weight?

<p>Micromolecules and macromolecules (B)</p> Signup and view all the answers

Lipids are classified as macromolecules despite having low molecular weights.

<p>True (A)</p> Signup and view all the answers

What is the primary component that makes up most of the cellular mass in living organisms?

<p>Water</p> Signup and view all the answers

Proteins are linear chains of amino acids linked by ______.

<p>peptide bonds</p> Signup and view all the answers

Match the following biomolecules with their percentage in the total cellular mass:

<p>Water = 70-90 Proteins = 10-15 Carbohydrates = 3 Lipids = 2 Nucleic acids = 5-7 Ions = 1</p> Signup and view all the answers

What type of biomolecule is primarily found in cell membranes?

<p>Lipids (B)</p> Signup and view all the answers

What is the function of proteins in biological systems?

<p>They serve as polypeptides that facilitate various biological functions.</p> Signup and view all the answers

Lipids are solely macromolecules in the context of biomolecules.

<p>False (B)</p> Signup and view all the answers

What is the term for the higher energy state that substrates must go through during a reaction?

<p>Transition state (C)</p> Signup and view all the answers

What complex is formed when an enzyme binds with its substrate?

<p>Enzyme-substrate complex (ES)</p> Signup and view all the answers

The difference in average energy content of the substrate (S) from that of the transition state is known as ______.

<p>activation energy</p> Signup and view all the answers

Match the following terms with their corresponding descriptions:

<p>Substrate (S) = The starting material for a reaction Product (P) = The result of the enzymatic reaction Enzyme (E) = The catalyst that speeds up the reaction Activated complex (ES) = Short-lived complex during the reaction</p> Signup and view all the answers

What occurs when the energy level of the product (P) is lower than that of the substrate (S) in a reaction?

<p>The reaction is exothermic. (D)</p> Signup and view all the answers

Enzymes raise the activation energy required for a reaction.

<p>False (B)</p> Signup and view all the answers

What is formed after the enzyme-substrate complex (ES) dissociates?

<p>Products (P) and unchanged enzyme (E)</p> Signup and view all the answers

Which of the following best describes a metabolic pathway?

<p>A series of chemical reactions with one or more final products (B)</p> Signup and view all the answers

Carbonic anhydrase is an enzyme that speeds up chemical reactions by a factor of one million times.

<p>False (B)</p> Signup and view all the answers

What is the role of the active site in an enzyme?

<p>The active site is where the substrate binds, allowing the enzyme to catalyze the reaction.</p> Signup and view all the answers

Under anaerobic conditions in muscles, glucose is converted to _____ acid.

<p>lactic</p> Signup and view all the answers

Match the enzyme activity with their corresponding conditions:

<p>Lactic Acid = Anaerobic conditions Pyruvic Acid = Aerobic conditions Ethanol = Fermentation Carbonic Anhydrase = Catalyzes CO2 and H2O conversion</p> Signup and view all the answers

Which statement correctly describes the transition state structure?

<p>It is a transient structure that occurs during substrate transformation. (A)</p> Signup and view all the answers

Enzymes completely change their structure when a substrate binds to the active site.

<p>False (B)</p> Signup and view all the answers

What is formed when the substrate binds to the enzyme at the active site?

<p>An enzyme-substrate (ES) complex</p> Signup and view all the answers

Which of the following elements is NOT typically found in living tissues?

<p>Silicon (B)</p> Signup and view all the answers

The dry weight of a living tissue is obtained after all water has evaporated from it.

<p>True (A)</p> Signup and view all the answers

What is left after burning all carbon compounds in a living tissue?

<p>Ash</p> Signup and view all the answers

Inorganic elements such as sodium and calcium are represented in the ______ of living tissues.

<p>ash</p> Signup and view all the answers

Match the following inorganic components with their formulas:

<p>Sodium = Na+ Calcium = Ca++ Magnesium = Mg++ Water = H2O</p> Signup and view all the answers

Which compound is formed when carbon compounds are fully oxidized?

<p>Carbon Dioxide (B)</p> Signup and view all the answers

Functional groups such as aldehydes and ketones can be identified in the analysis of living tissues.

<p>True (A)</p> Signup and view all the answers

What is one method used to analyze the composition of living tissues?

<p>Elemental analysis</p> Signup and view all the answers

Which type of amino acids cannot be synthesized by the body and must be obtained from the diet?

<p>Essential amino acids (A)</p> Signup and view all the answers

Collagen is the most abundant protein in the animal kingdom.

<p>True (A)</p> Signup and view all the answers

Name one function of enzymes.

<p>Catalyzing biochemical reactions</p> Signup and view all the answers

The protein that fights infectious agents is known as an _______.

<p>Antibody</p> Signup and view all the answers

Match the following proteins with their primary functions:

<p>Insulin = Hormone regulation Trypsin = Digestive enzyme Collagen = Structural support GLUT-4 = Glucose transport</p> Signup and view all the answers

Which of the following polysaccharides serves as an energy storage molecule in animals?

<p>Glycogen (B)</p> Signup and view all the answers

Starch and cellulose are both homopolymers made up of glucose.

<p>False (B)</p> Signup and view all the answers

What are the two types of amino acids based on dietary needs?

<p>Essential and non-essential amino acids</p> Signup and view all the answers

What role does carbonic anhydrase play in chemical reactions?

<p>It speeds up reactions. (C)</p> Signup and view all the answers

Every enzyme catalyzes multiple types of metabolic reactions.

<p>False (B)</p> Signup and view all the answers

What is the term for the complex formed when a substrate binds to an enzyme?

<p>ES complex</p> Signup and view all the answers

Under anaerobic conditions in skeletal muscle, ______ is formed.

<p>lactic acid</p> Signup and view all the answers

What type of polysaccharide is cellulose primarily known for forming?

<p>Plant cell walls (A)</p> Signup and view all the answers

Match the metabolic end products with their conditions:

<p>Lactic acid = Anaerobic conditions Pyruvic acid = Aerobic conditions Ethanol = Fermentation Glucose = Starting substrate</p> Signup and view all the answers

Starch can hold iodine molecules due to its helical structure.

<p>True (A)</p> Signup and view all the answers

What is the outcome when glucose is converted through a metabolic pathway?

<p>It can lead to different products based on conditions. (B)</p> Signup and view all the answers

What are the building blocks of nucleic acids?

<p>Nucleotides</p> Signup and view all the answers

Enzymes transform substrates into products by creating a stable intermediate state.

<p>False (B)</p> Signup and view all the answers

How does an enzyme convert a substrate into a product?

<p>The enzyme binds the substrate at its active site, forming a transition state, and then releases the product.</p> Signup and view all the answers

Chitin, a complex polysaccharide, is found in the exoskeletons of ______.

<p>arthropods</p> Signup and view all the answers

Match the following components with their respective nucleic acids:

<p>Deoxyribonucleic acid = Contains deoxyribose Ribonucleic acid = Contains ribose Adenine = Purine base Cytosine = Pyrimidine base</p> Signup and view all the answers

Which nitrogenous base is classified as a pyrimidine?

<p>Thymine (B)</p> Signup and view all the answers

Ribose is a pentose monosaccharide found in DNA.

<p>False (B)</p> Signup and view all the answers

Name one type of structure that proteins can form.

<p>Tertiary structure</p> Signup and view all the answers

What is the term for the energy barrier that substrates must overcome during a reaction?

<p>Activation energy (D)</p> Signup and view all the answers

All intermediate structural states between a substrate and product are stable.

<p>False (B)</p> Signup and view all the answers

What complex is formed between an enzyme and substrate before the product is formed?

<p>Enzyme-substrate complex (ES)</p> Signup and view all the answers

The product (P) and the unchanged enzyme dissociate after the complex known as ______.

<p>enzyme-product complex (EP)</p> Signup and view all the answers

Match the following components of enzyme action:

<p>E = Enzyme S = Substrate ES = Enzyme-substrate complex P = Product</p> Signup and view all the answers

In an exothermic reaction, which statement is true about the energy level of the product compared to the substrate?

<p>The product is at a lower energy level than the substrate. (B)</p> Signup and view all the answers

Enzymes increase the activation energy required for a reaction to occur.

<p>False (B)</p> Signup and view all the answers

What is implied if the energy level of the product (P) is lower than that of the substrate (S)?

<p>The reaction is exothermic.</p> Signup and view all the answers

Which of the following best describes the role of ligases?

<p>Catalyzing the linking together of two compounds (B)</p> Signup and view all the answers

Prosthetic groups are temporarily attached to apoenzymes.

<p>False (B)</p> Signup and view all the answers

What is the name given to the protein portion of an enzyme that requires a cofactor to become active?

<p>apoenzyme</p> Signup and view all the answers

Zinc acts as a _______ for the enzyme carboxypeptidase.

<p>cofactor</p> Signup and view all the answers

Match the following types of cofactors with their descriptions:

<p>Prosthetic group = Tightly bound to apoenzyme Co-enzyme = Transient association with apoenzyme Metal ion = Required for enzyme activity and forms coordination bonds</p> Signup and view all the answers

Which of the following statements about coenzymes is correct?

<p>They serve as cofactors in various reactions (B)</p> Signup and view all the answers

The removal of a cofactor from an enzyme will not affect its catalytic activity.

<p>False (B)</p> Signup and view all the answers

Name one example of a coenzyme that contains a vitamin.

<p>NAD (nicotinamide adenine dinucleotide)</p> Signup and view all the answers

Which of the following substances is a nucleotide?

<p>Adenylic acid (A)</p> Signup and view all the answers

DNA and RNA are types of nucleotides.

<p>False (B)</p> Signup and view all the answers

What are the two types of nitrogenous bases found in nucleic acids?

<p>Purines and pyrimidines</p> Signup and view all the answers

A molecule consisting of a nitrogen base and a sugar is called a ______.

<p>nucleoside</p> Signup and view all the answers

Match the following nitrogen bases with their corresponding type:

<p>Adenine = Purine Uracil = Pyrimidine Guanine = Purine Cytosine = Pyrimidine</p> Signup and view all the answers

Which of the following are considered nucleosides? (Select all that apply)

<p>Thymidine (C), Adenosine (D)</p> Signup and view all the answers

All nucleotides contain the same sugar component.

<p>False (B)</p> Signup and view all the answers

Name one primary function of DNA and RNA.

<p>Storage and transmission of genetic information</p> Signup and view all the answers

Which elements are typically found in higher relative abundance in living organisms compared to the earth's crust?

<p>Carbon and Hydrogen (A)</p> Signup and view all the answers

The acid-soluble pool contains fewer organic compounds than the acid-insoluble fraction.

<p>False (B)</p> Signup and view all the answers

What does the process of grinding living tissue in trichloroacetic acid help to analyze?

<p>The chemical composition of organic compounds.</p> Signup and view all the answers

The two fractions obtained after grinding tissue in acid are called __________ and __________.

<p>filtrate, retentate</p> Signup and view all the answers

Match the following elements with their respective weight percentage in the human body:

<p>Hydrogen = 0.5% Carbon = 18.5% Oxygen = 65% Nitrogen = 3%</p> Signup and view all the answers

When comparing living tissues to non-living matter, what can be said about the elemental composition?

<p>All elements found in living tissues are also present in non-living matter. (D)</p> Signup and view all the answers

Carbon is present in the earth's crust at a higher percentage than in the human body.

<p>False (B)</p> Signup and view all the answers

What are the two main types of fractions obtained after chemical analysis of living tissues?

<p>Acid-soluble pool and acid-insoluble fraction.</p> Signup and view all the answers

Cellulose can form helical structures similar to starch.

<p>False (B)</p> Signup and view all the answers

Name a complex polysaccharide found in the exoskeletons of arthropods.

<p>Chitin</p> Signup and view all the answers

The sugar component in RNA is ______.

<p>ribose</p> Signup and view all the answers

Match the following structures with their descriptions:

<p>Starch = Energy storage polysaccharide in plants Cellulose = Structural component of plant cell walls RNA = Contains ribose DNA = Contains deoxyribose</p> Signup and view all the answers

Which nitrogenous base is a pyrimidine?

<p>Cytosine (A)</p> Signup and view all the answers

Amino acids are the building blocks of nucleic acids.

<p>False (B)</p> Signup and view all the answers

What is the main component of the acid-insoluble fraction in living tissues?

<p>Nucleic acids</p> Signup and view all the answers

What is the term for the 3-dimensional structure of a protein formed by the folding of its polypeptide chains?

<p>Tertiary structure (D)</p> Signup and view all the answers

The quaternary structure of a protein involves a single polypeptide chain.

<p>False (B)</p> Signup and view all the answers

Human haemoglobin consists of ______ subunits.

<p>4</p> Signup and view all the answers

Match the following types of protein structure with their descriptions:

<p>Primary Structure = Amino acid sequence Secondary Structure = Alpha helices and beta sheets Tertiary Structure = Folding into 3D shape Quaternary Structure = Assembly of multiple polypeptides</p> Signup and view all the answers

What kind of RNA acts as an enzyme?

<p>Ribozymes (D)</p> Signup and view all the answers

All enzymes are proteins.

<p>False (B)</p> Signup and view all the answers

What is formed by the folding and arrangement of individual polypeptide chains in proteins?

<p>Quaternary structure</p> Signup and view all the answers

What term describes the first amino acid in a protein structure?

<p>N-terminal amino acid (B)</p> Signup and view all the answers

The secondary structure of proteins can include left-handed helices.

<p>False (B)</p> Signup and view all the answers

What are the two extreme ends of a protein structure known as?

<p>N-terminal and C-terminal</p> Signup and view all the answers

The ____ structure of proteins describes how the polypeptide folds into a helical form.

<p>secondary</p> Signup and view all the answers

Which level of protein structure involves the arrangement of the polypeptide into a helix or sheet?

<p>Secondary (D)</p> Signup and view all the answers

The primary structure of a protein is defined by its shape and folding.

<p>False (B)</p> Signup and view all the answers

What type of bond is significant for maintaining the tertiary structure of proteins?

<p>Disulphide bond</p> Signup and view all the answers

What is the state called that the substrate must pass through before becoming the product?

<p>Transition state (C)</p> Signup and view all the answers

The energy level of the product is always lower than that of the substrate in an exothermic reaction.

<p>True (A)</p> Signup and view all the answers

What is the term for the energy difference between the stable substrate and the transition state?

<p>Activation energy</p> Signup and view all the answers

The complex formed between an enzyme and substrate during a reaction is known as the ______ complex.

<p>enzyme-substrate</p> Signup and view all the answers

Match the following terms with their descriptions:

<p>Substrate (S) = Starting material in a reaction Product (P) = Result of a chemical reaction Activation energy = Energy required to start a reaction Enzyme = Biological catalyst that speeds up reactions</p> Signup and view all the answers

What effect do enzymes have on activation energy?

<p>Decrease it (B)</p> Signup and view all the answers

The enzyme-product complex (EP) is a long-lived structure during the catalytic process.

<p>False (B)</p> Signup and view all the answers

What must occur before a substrate can be transformed into product via an enzyme?

<p>The substrate must bind to the active site of the enzyme to form the enzyme-substrate complex.</p> Signup and view all the answers

Which of the following nitrogen bases are NOT found in DNA?

<p>Uracil (B)</p> Signup and view all the answers

Nucleotides are composed of a nitrogenous base, a sugar, and a phosphate group.

<p>True (A)</p> Signup and view all the answers

What are the two types of nucleic acids?

<p>DNA and RNA</p> Signup and view all the answers

Adenosine is a type of _______.

<p>nucleoside</p> Signup and view all the answers

Match the following nitrogenous bases with their classification:

<p>Adenine = Purine Uracil = Pyrimidine Guanine = Purine Cytosine = Pyrimidine</p> Signup and view all the answers

Which of the following compounds are classified as nucleotides?

<p>Adenylic acid (D)</p> Signup and view all the answers

All nucleotides contain a phosphate group attached to a nitrogenous base and a sugar.

<p>True (A)</p> Signup and view all the answers

What is the role of nucleic acids in living organisms?

<p>Genetic material</p> Signup and view all the answers

Which of the following is a characteristic of amino acids?

<p>They contain a nitrogen group and a carboxyl group. (D)</p> Signup and view all the answers

All amino acids that occur in proteins are of the same type.

<p>False (B)</p> Signup and view all the answers

What type of fatty acid has no double bonds?

<p>Saturated fatty acid</p> Signup and view all the answers

Palmitic acid contains ___ carbon atoms, including the carboxyl carbon.

<p>16</p> Signup and view all the answers

Match the amino acid with its R group:

<p>Glycine = Hydrogen Alanine = Methyl Serine = Hydroxymethyl Lysine = Amino group</p> Signup and view all the answers

Lipids are generally water-soluble.

<p>False (B)</p> Signup and view all the answers

What is the zwitterionic form of an amino acid?

<p>The state where the amino acid has both a positive and a negative charge.</p> Signup and view all the answers

What type of protein is the most abundant in the animal world?

<p>Collagen (A)</p> Signup and view all the answers

Essential amino acids can be synthesized by the body.

<p>False (B)</p> Signup and view all the answers

What are polysaccharides primarily made of?

<p>Monosaccharides</p> Signup and view all the answers

____ is a polymer of fructose.

<p>Inulin</p> Signup and view all the answers

Which of the following functions does insulin perform?

<p>Hormonal regulation (C)</p> Signup and view all the answers

Match the following types of proteins with their functions:

<p>Collagen = Structural support Trypsin = Catalyzes reactions Glucose transporters = Facilitates nutrient transport Antibodies = Immune response</p> Signup and view all the answers

Glycogen is a homopolymer of glucose.

<p>True (A)</p> Signup and view all the answers

The left end of a polysaccharide chain is referred to as the ____ end.

<p>non-reducing</p> Signup and view all the answers

What characterizes the transition state in a chemical reaction?

<p>It requires high activation energy to form. (D)</p> Signup and view all the answers

What is the term for the temporary complex formed when an enzyme binds with its substrate?

<p>enzyme-substrate complex</p> Signup and view all the answers

The difference in energy content between the substrate and the transition state is referred to as ______.

<p>activation energy</p> Signup and view all the answers

Which statement is true regarding enzymes?

<p>Enzymes can form a short-lived enzyme-product complex. (B)</p> Signup and view all the answers

An enzyme's active site is specific to only one substrate.

<p>True (A)</p> Signup and view all the answers

In the catalytic cycle, the enzyme returns to its original state after producing the ______.

<p>product</p> Signup and view all the answers

What is the term for the maximum velocity an enzyme can reach when substrate concentration increases?

<p>Vmax (C)</p> Signup and view all the answers

Enzymes can function effectively outside a narrow range of temperature and pH.

<p>False (B)</p> Signup and view all the answers

What happens to enzyme activity at low temperatures?

<p>Enzyme becomes temporarily inactive.</p> Signup and view all the answers

The change in the shape of an enzyme upon substrate binding is known as ______.

<p>induced fit</p> Signup and view all the answers

Match the factors affecting enzyme activity with their effects:

<p>High temperature = Denatures the enzyme Low temperature = Temporarily inactive enzyme Change in pH = Alters enzyme's tertiary structure Substrate concentration increase = Increases reaction velocity until Vmax</p> Signup and view all the answers

What occurs when substrate concentration increases and exceeds the number of available enzyme molecules?

<p>Reaction velocity reaches a maximum (D)</p> Signup and view all the answers

The active site of an enzyme is where the enzyme fits tightly around the substrate.

<p>True (A)</p> Signup and view all the answers

Name one specific chemical factor that can regulate enzyme activity?

<p>Inhibitors or activators.</p> Signup and view all the answers

What are the three identified types of cofactors?

<p>Prosthetic groups, co-enzymes, metal ions (A)</p> Signup and view all the answers

Prosthetic groups are loosely attached to the enzyme and can be easily removed.

<p>False (B)</p> Signup and view all the answers

What is the role of coenzymes during enzyme catalysis?

<p>They serve as co-factors and associate transiently with the apoenzyme.</p> Signup and view all the answers

The essential chemical components of many coenzymes are derived from ______.

<p>vitamins</p> Signup and view all the answers

What happens to an enzyme when its co-factor is removed?

<p>It loses its catalytic activity. (A)</p> Signup and view all the answers

Match the following enzyme types with their functions:

<p>Ligases = Join two compounds together Hydrolases = Catalyze hydrolysis reactions Oxidoreductases = Catalyze oxidation-reduction reactions Transferases = Transfer functional groups between substrates</p> Signup and view all the answers

Water is the most abundant chemical in living organisms.

<p>True (A)</p> Signup and view all the answers

What portion of an enzyme is referred to as the apoenzyme?

<p>The protein portion of the enzyme without its co-factor.</p> Signup and view all the answers

What type of polymer are proteins classified as?

<p>Heteropolymer (D)</p> Signup and view all the answers

Essential amino acids can be synthesized by the human body.

<p>False (B)</p> Signup and view all the answers

Name one function of insulin.

<p>Hormone regulation of blood sugar</p> Signup and view all the answers

Cellulose is a polymer of __________.

<p>glucose</p> Signup and view all the answers

Match the following proteins with their functions:

<p>Collagen = Intercellular ground substance Trypsin = Enzyme Insulin = Hormone Antibody = Fights infectious agents</p> Signup and view all the answers

Which of the following is a polysaccharide that serves as energy storage in plants?

<p>Starch (C)</p> Signup and view all the answers

Ribulose bisphosphate Carboxylase-Oxygenase (RuBisCO) is the most abundant protein in the biosphere.

<p>True (A)</p> Signup and view all the answers

What is the non-reducing end of a polysaccharide chain?

<p>The left end of the chain</p> Signup and view all the answers

Which of the following compounds is classified as a secondary metabolite?

<p>Rubber (B)</p> Signup and view all the answers

Primary metabolites play known roles in normal physiological processes.

<p>True (A)</p> Signup and view all the answers

Name one example of a primary metabolite.

<p>Amino acid</p> Signup and view all the answers

The molecular weights of secondary metabolites typically range from _____ to around 800 daltons.

<p>18</p> Signup and view all the answers

Match the following secondary metabolites with their examples:

<p>Alkaloids = Morphine Terpenoids = Lemon grass oil Pigments = Anthocyanins Toxins = Ricin</p> Signup and view all the answers

Which of the following is NOT a characteristic of secondary metabolites?

<p>They have identifiable functions. (D)</p> Signup and view all the answers

All compounds in the acid soluble pool are primary metabolites.

<p>False (B)</p> Signup and view all the answers

What are the four types of organic compounds found in the acid insoluble fraction?

<p>Proteins, nucleic acids, polysaccharides, lipids</p> Signup and view all the answers

What is the primary compound used for grinding living tissue to analyze its chemical composition?

<p>Trichloroacetic acid (C)</p> Signup and view all the answers

The relative abundance of elements in living tissues is lower compared to that in the earth's crust.

<p>False (B)</p> Signup and view all the answers

What are the two fractions obtained when living tissue is processed with trichloroacetic acid?

<p>Filtrate and retentate</p> Signup and view all the answers

The process of separating organic compounds from a mixture is known as __________.

<p>extraction</p> Signup and view all the answers

Match the elements with their respective percentages in the human body as compared to the earth's crust.

<p>Hydrogen (H) = 0.5% Carbon (C) = 18.5% Oxygen (O) = 28% Nitrogen (N) = 3.2%</p> Signup and view all the answers

Which of the following elements is NOT found in significant amounts in living organisms?

<p>Argon (B)</p> Signup and view all the answers

All elements present in living organisms can also be found in the earth's crust.

<p>True (A)</p> Signup and view all the answers

What is the name of the pool that contains organic compounds obtained from the filtrate after processing tissue?

<p>Acid-soluble pool</p> Signup and view all the answers

What type of macromolecule is made up of nucleotides?

<p>Nucleic acids (C)</p> Signup and view all the answers

The complex polysaccharide found in arthropods is called ______.

<p>chitin</p> Signup and view all the answers

Which of the following sugars is a component of RNA?

<p>Ribose (C)</p> Signup and view all the answers

Cellulose contains complex helices similar to starch.

<p>False (B)</p> Signup and view all the answers

What is a major difference between enzyme catalysts and inorganic catalysts?

<p>Enzymes do not work efficiently at high temperatures. (C)</p> Signup and view all the answers

Enzymes isolated from thermophilic organisms can maintain their catalytic activity at temperatures up to 90°C.

<p>True (A)</p> Signup and view all the answers

What is the term for the process when bonds are broken and new bonds are formed during a transformation?

<p>chemical reaction</p> Signup and view all the answers

The rate of a physical or chemical process refers to the amount of product formed per unit time, expressed as δP/δt = ______.

<p>rate</p> Signup and view all the answers

Match the enzyme-catalyzed reaction with its corresponding reactants:

<p>Carbonic anhydrase = CO2 and H2O Bromelain = Proteins Lipase = Triglycerides Amylase = Starch</p> Signup and view all the answers

Which of the following factors can influence the rate of a chemical reaction?

<p>Temperature (D)</p> Signup and view all the answers

How does the rate of a reaction typically change with a 10°C change in temperature, assuming all other factors remain constant?

<p>Doubles or decreases by half</p> Signup and view all the answers

Enzyme-catalyzed reactions proceed at rates that are generally slower than uncatalyzed ones.

<p>False (B)</p> Signup and view all the answers

What describes the effect of increasing substrate concentration on enzyme activity?

<p>It increases activity until Vmax is reached. (B)</p> Signup and view all the answers

Enzymes function optimally at a wide range of temperatures and pH levels.

<p>False (B)</p> Signup and view all the answers

What is the term for the maximum rate of an enzymatic reaction?

<p>Vmax</p> Signup and view all the answers

The shape change of an enzyme induced by substrate binding is referred to as the ______ effect.

<p>induced fit</p> Signup and view all the answers

Match the following factors with their effect on enzymes:

<p>High temperature = Denatures proteins Low temperature = Slows enzymatic activity Optimal pH = Maximizes enzyme activity Extreme pH = Denatures proteins</p> Signup and view all the answers

What happens to an enzyme after it catalyzes a reaction?

<p>It is released and can catalyze again. (B)</p> Signup and view all the answers

What is the effect of high temperature on enzymes?

<p>Denaturation</p> Signup and view all the answers

Enzymatic activity increases indefinitely with an increase in substrate concentration.

<p>False (B)</p> Signup and view all the answers

All fatty acids contain a carboxyl group and R group.

<p>True (A)</p> Signup and view all the answers

What type of sugar is found in ribonucleic acid (RNA)?

<p>Ribose (D)</p> Signup and view all the answers

Name an example of an aromatic amino acid.

<p>Tyrosine</p> Signup and view all the answers

Starch and cellulose share similar complex helical structures.

<p>False (B)</p> Signup and view all the answers

Arachidonic acid has ______ carbon atoms including the carboxyl carbon.

<p>20</p> Signup and view all the answers

Match the following types of amino acids with their classification:

<p>Glutamic acid = Acidic Lysine = Basic Valine = Neutral Tyrosine = Aromatic</p> Signup and view all the answers

Identify a complex polysaccharide found in the exoskeletons of arthropods.

<p>Chitin</p> Signup and view all the answers

The building blocks of nucleic acids are known as __________.

<p>nucleotides</p> Signup and view all the answers

Which of these best describes the zwitterionic form of amino acids?

<p>Has both positive and negative charges (D)</p> Signup and view all the answers

Match the nucleic acid with its sugar component:

<p>DNA = Deoxyribose RNA = Ribose</p> Signup and view all the answers

Phospholipids are primarily found in plant cell walls.

<p>False (B)</p> Signup and view all the answers

What is the primary structure of fatty acids based on the presence of double bonds?

<p>Saturated and unsaturated</p> Signup and view all the answers

Glucosamine is a monosaccharide component of nucleic acids.

<p>False (B)</p> Signup and view all the answers

Name the type of bond that links amino acids together in proteins.

<p>Peptide bond</p> Signup and view all the answers

What is one key difference between enzyme catalysts and inorganic catalysts?

<p>Enzymes are damaged at high temperatures while inorganic catalysts are not. (A)</p> Signup and view all the answers

Enzymes from thermophilic organisms are stable at temperatures up to 90°C.

<p>True (A)</p> Signup and view all the answers

What happens to the bonds during a chemical reaction?

<p>Bonds are broken and new bonds are formed.</p> Signup and view all the answers

The rate of a chemical process can be expressed as ______.

<p>δP/δt</p> Signup and view all the answers

Which factor can influence the rate of a chemical or physical process?

<p>Temperature and other factors (D)</p> Signup and view all the answers

Match the following enzyme-catalyzed reactions with their corresponding products:

<p>Carbonic anhydrase = HCO3- Hydrolysis of starch = Glucose Ba(OH)2 + H2SO4 = BaSO4 + H2O Enzymatic reactions = Higher reaction rates</p> Signup and view all the answers

Catalyzed reactions generally proceed at rates lower than uncatalyzed ones.

<p>False (B)</p> Signup and view all the answers

How does the rate of a chemical reaction typically change with a 10°C temperature change?

<p>The rate doubles or decreases by half.</p> Signup and view all the answers

What is the term used to describe the high energy state that substrates transition through during a reaction?

<p>Transition state (B)</p> Signup and view all the answers

The activation energy for a reaction does not change when an enzyme is present.

<p>False (B)</p> Signup and view all the answers

What complex is formed when a substrate binds to an enzyme?

<p>Enzyme-substrate complex (ES)</p> Signup and view all the answers

The energy level of the product (P) is lower than the substrate (S) in an _______ reaction.

<p>exothermic</p> Signup and view all the answers

Which of the following statements is true regarding the stability of intermediate structural states?

<p>They are unstable and cannot exist long-term. (A)</p> Signup and view all the answers

An enzyme is unchanged after catalyzing a reaction.

<p>True (A)</p> Signup and view all the answers

What must a substrate (S) do in order to convert into a product (P)?

<p>Go through a high energy transition state.</p> Signup and view all the answers

What is the primary structure of an enzyme?

<p>Linear sequence of amino acids (C)</p> Signup and view all the answers

The quaternary structure of a protein involves multiple polypeptide subunits.

<p>True (A)</p> Signup and view all the answers

What is the active site of an enzyme?

<p>A pocket or crevice where the substrate fits.</p> Signup and view all the answers

Hemoglobin in adult humans consists of _____ subunits.

<p>4</p> Signup and view all the answers

What happens to the structure of a protein when it folds into its tertiary form?

<p>It forms a complex 3D shape with crevices and pockets.</p> Signup and view all the answers

What is a competitive inhibitor known for?

<p>It resembles the substrate and competes for the enzyme's binding site. (D)</p> Signup and view all the answers

Enzymes that catalyze hydrolysis reactions are classified as transferases.

<p>False (B)</p> Signup and view all the answers

What enzyme is inhibited by the competitive inhibitor malonate?

<p>succinic dehydrogenase</p> Signup and view all the answers

Enzymes are classified into _____ major classes based on the type of reactions they catalyze.

<p>6</p> Signup and view all the answers

Match the class of enzyme with its function:

<p>Oxidoreductases = Catalyze oxidation-reduction reactions Transferases = Catalyze the transfer of groups between substrates Hydrolases = Catalyze hydrolysis reactions Lyases = Catalyze the removal of groups to form double bonds</p> Signup and view all the answers

Which of the following describes the function of isomerases?

<p>They catalyze the interconversion of isomers. (A)</p> Signup and view all the answers

Name one example of a reaction type that lyases catalyze.

<p>Removal of a group to form a double bond</p> Signup and view all the answers

What are the nitrogen bases found in nucleic acids?

<p>Adenine, Guanine, Cytosine, Uracil, Thymine (A)</p> Signup and view all the answers

All nucleotides consist of a sugar, a nitrogenous base, and a phosphate group.

<p>True (A)</p> Signup and view all the answers

What are adenosine and guanosine classified as?

<p>Nucleosides</p> Signup and view all the answers

The metabolic compounds that consist of nucleotides are called ______.

<p>Nucleic acids</p> Signup and view all the answers

Match the types of nucleic acids with their primary component:

<p>DNA = Deoxyribose RNA = Ribose Adenylic acid = Adenine Thymidylic acid = Thymine</p> Signup and view all the answers

Which of the following is NOT a component of a nucleotide?

<p>Fatty acid (B)</p> Signup and view all the answers

Thymine is found only in RNA.

<p>False (B)</p> Signup and view all the answers

Name one type of nucleic acid.

<p>DNA or RNA</p> Signup and view all the answers

Which of the following are classified as primary metabolites?

<p>Amino acids (D)</p> Signup and view all the answers

Secondary metabolites have known roles in normal physiological processes.

<p>False (B)</p> Signup and view all the answers

The molecular weights of primary metabolites typically range from _____ to around 800 daltons.

<p>18</p> Signup and view all the answers

Match the following types of metabolites with their examples:

<p>Primary metabolites = Amino acids Secondary metabolites = Alkaloids Pigments = Anthocyanins Essential oils = Lemon grass oil</p> Signup and view all the answers

What is the typical molecular weight range for proteins, nucleic acids, and polysaccharides?

<p>10,000 daltons and above (D)</p> Signup and view all the answers

Animal tissues contain only primary metabolites.

<p>True (A)</p> Signup and view all the answers

What are compounds with a molecular weight of more than 800 daltons generally classified as?

<p>Proteins, nucleic acids, polysaccharides, and lipids</p> Signup and view all the answers

What type of protein acts as a hormone and helps regulate blood sugar levels?

<p>Insulin (A)</p> Signup and view all the answers

What is the most abundant protein in the animal kingdom?

<p>Collagen</p> Signup and view all the answers

Cellulose is a homopolymer made up of _______ as its monomer.

<p>glucose</p> Signup and view all the answers

Which of the following polysaccharides serves as a storage form of energy in animals?

<p>Glycogen (C)</p> Signup and view all the answers

All amino acids are considered essential for human health.

<p>False (B)</p> Signup and view all the answers

What is the function of GLUT-4 protein?

<p>Enables glucose transport into cells</p> Signup and view all the answers

What type of inhibitor resembles the substrate and competes for the enzyme's binding site?

<p>Competitive inhibitor (C)</p> Signup and view all the answers

Enzymes are classified into four classes based on the type of reactions they catalyze.

<p>False (B)</p> Signup and view all the answers

The process by which enzyme activity is shut off by a chemical is called ______.

<p>inhibition</p> Signup and view all the answers

What is the primary function of isomerases?

<p>Inter-conversion of isomers (A)</p> Signup and view all the answers

Hydrolases catalyze the removal of groups from substrates leaving double bonds.

<p>False (B)</p> Signup and view all the answers

What does the term Km refer to in enzyme kinetics?

<p>Michaelis constant</p> Signup and view all the answers

Thermophilic enzymes can retain their catalytic power at temperatures up to 90°C.

<p>True (A)</p> Signup and view all the answers

What happens to the rate of a chemical reaction with every 10°C change in temperature?

<p>The rate doubles or decreases by half.</p> Signup and view all the answers

The reaction of carbon dioxide and water to form carbonic acid is catalyzed by the enzyme ______.

<p>carbonic anhydrase</p> Signup and view all the answers

Match the following temperature ranges with their effects on enzyme activity:

<p>Below 40°C = Enzyme activity is optimal 40°C - 60°C = Enzyme activity starts to decline Above 60°C = Denaturation occurs Thermophilic range (80°-90°C) = Enzymes retain catalytic power</p> Signup and view all the answers

Which statement correctly describes a chemical reaction?

<p>It leads to new substances being formed. (A)</p> Signup and view all the answers

The rate of a reaction is unaffected by temperature changes.

<p>False (B)</p> Signup and view all the answers

What type of change does the melting of ice into water represent?

<p>Physical change</p> Signup and view all the answers

What happens to the enzyme after it releases the products of a reaction?

<p>It is ready to bind to another substrate molecule. (B)</p> Signup and view all the answers

Enzymes only function in a wide range of temperature and pH.

<p>False (B)</p> Signup and view all the answers

What is the term for the maximum velocity that an enzymatic reaction can achieve?

<p>Vmax</p> Signup and view all the answers

Enzymes are sensitive to the presence of specific chemicals that can bind to the enzyme, affecting its _____ activity.

<p>catalytic</p> Signup and view all the answers

Match each condition affecting enzyme activity with its effect:

<p>High temperature = Denaturation of the enzyme Low temperature = Temporary inactivity Optimum pH = Highest enzyme activity High substrate concentration = Reaches Vmax</p> Signup and view all the answers

Increasing substrate concentration increases the velocity of an enzymatic reaction until which point?

<p>The reaction reaches Vmax. (D)</p> Signup and view all the answers

The structure of an enzyme is unaffected by changes in temperature and pH.

<p>False (B)</p> Signup and view all the answers

What occurs to an enzyme at low temperatures?

<p>It becomes temporarily inactive.</p> Signup and view all the answers

Which component is NOT part of a nucleotide?

<p>Fatty acid (A)</p> Signup and view all the answers

Cellulose contains more complex helices than starch.

<p>False (B)</p> Signup and view all the answers

What is the sugar component found in DNA?

<p>2' deoxyribose</p> Signup and view all the answers

Chitin is a complex polysaccharide found in the _______ of arthropods.

<p>exoskeleton</p> Signup and view all the answers

Match the following nutrients with their descriptions:

<p>Nucleic acids = Polymers made of nucleotides Polysaccharides = Carbohydrates formed by many sugar molecules Proteins = Polymers made of amino acids Lipids = Fats that are not soluble in water</p> Signup and view all the answers

Which of the following is a nitrogenous base found in RNA?

<p>Uracil (A)</p> Signup and view all the answers

A nucleotide is comprised of a monosaccharide, a heterocyclic compound, and a _______.

<p>phosphoric acid</p> Signup and view all the answers

Starch can hold iodine molecules in its helical structure.

<p>True (A)</p> Signup and view all the answers

What level of protein structure is characterized by the assembly of more than one polypeptide chain?

<p>Quaternary structure (B)</p> Signup and view all the answers

The active site of an enzyme is where the enzyme forms its primary structure.

<p>False (B)</p> Signup and view all the answers

The structure of a protein that appears as a hollow woolen ball is called the ______ structure.

<p>tertiary</p> Signup and view all the answers

Which of the following statements is true about enzymes?

<p>All enzymes are proteins. (D)</p> Signup and view all the answers

Ribozymes are nucleic acids that behave like proteins.

<p>True (A)</p> Signup and view all the answers

The part of the enzyme where the substrate fits is referred to as the ______ site.

<p>active</p> Signup and view all the answers

What defines a chemical reaction?

<p>The breaking of bonds and formation of new ones (B)</p> Signup and view all the answers

Enzymes can function effectively at temperatures above 90°C.

<p>False (B)</p> Signup and view all the answers

What is the primary role of enzyme catalysts?

<p>To speed up chemical reactions.</p> Signup and view all the answers

A __________ reaction occurs when bonds are broken and formed during a transformation.

<p>chemical</p> Signup and view all the answers

Match the following types of reactions with their characteristics:

<p>Physical change = Change in shape or state without breaking bonds Chemical reaction = Breaking and forming of bonds Uncatalyzed reaction = Slow reaction without an enzyme Catalyzed reaction = Rapid reaction due to enzyme presence</p> Signup and view all the answers

What happens to the rate of a reaction for every 10°C temperature change?

<p>It doubles or halves (A)</p> Signup and view all the answers

Inorganic catalysts require lower temperatures and pressures compared to enzymes.

<p>False (B)</p> Signup and view all the answers

What is the thermal stability of enzymes from thermophilic organisms?

<p>They retain catalytic power at high temperatures up to 80°-90°C.</p> Signup and view all the answers

What is a metabolic pathway?

<p>A sequence of chemical reactions catalyzed by enzymes (C)</p> Signup and view all the answers

The enzyme carbonic anhydrase slows down the reaction rate of biochemical processes.

<p>False (B)</p> Signup and view all the answers

What do you call the chemical that is converted into a product by an enzyme?

<p>substrate</p> Signup and view all the answers

In skeletal muscle, lactic acid is produced under _____ conditions.

<p>anaerobic</p> Signup and view all the answers

Match the following conditions with their corresponding end products in the metabolic pathway:

<p>Anaerobic conditions = Lactic acid Aerobic conditions = Pyruvic acid Fermentation in yeast = Ethanol Normal conditions = Glucose</p> Signup and view all the answers

What role does the active site of an enzyme play?

<p>It is where the substrate binds and reaction occurs (A)</p> Signup and view all the answers

Enzymes remain unchanged after catalyzing a reaction.

<p>True (A)</p> Signup and view all the answers

What is the transient complex formed when a substrate binds to an enzyme called?

<p>ES complex</p> Signup and view all the answers

What is the term for the energy required to reach the transition state from the substrate?

<p>Activation energy (A)</p> Signup and view all the answers

The substrate (S) can directly convert to the product (P) without going through the transition state.

<p>False (B)</p> Signup and view all the answers

What is formed when an enzyme binds to a substrate?

<p>Enzyme-substrate complex (ES)</p> Signup and view all the answers

In an exothermic reaction, the product (P) is at a ______ energy level than the substrate (S).

<p>lower</p> Signup and view all the answers

What complex is produced shortly after the substrate binds to the enzyme?

<p>Enzyme-product complex (EP)</p> Signup and view all the answers

What is the optimum condition for an enzyme's activity?

<p>Optimum temperature and optimum pH (C)</p> Signup and view all the answers

High temperatures can preserve enzyme activity.

<p>False (B)</p> Signup and view all the answers

The maximum velocity of an enzymatic reaction is known as ______.

<p>Vmax</p> Signup and view all the answers

What effect does low temperature have on enzymes?

<p>Preserves the enzyme in an inactive state (D)</p> Signup and view all the answers

An increase in substrate concentration will always lead to an increase in enzymatic reaction velocity.

<p>False (B)</p> Signup and view all the answers

What is the structural change that occurs when a substrate binds to an enzyme?

<p>The enzyme alters its shape to fit more tightly around the substrate.</p> Signup and view all the answers

What common elements are found in both living tissues and the earth's crust?

<p>Carbon and Hydrogen (C)</p> Signup and view all the answers

The relative abundance of carbon and hydrogen is greater in living organisms than in the earth's crust.

<p>True (A)</p> Signup and view all the answers

What is the purpose of grinding living tissue in trichloroacetic acid?

<p>To perform a chemical analysis and separate organic compounds.</p> Signup and view all the answers

Living organisms consist of elements like _____, _____, and several others.

Signup and view all the answers

Which of the following is considered a class of amino acids based on their R group?

<p>Neutral amino acids (C)</p> Signup and view all the answers

Fatty acids are typically water soluble.

<p>False (B)</p> Signup and view all the answers

Amino acids containing an ionizable –NH2 and –COOH group can exist in a structure called a ______ form.

<p>zwitterionic</p> Signup and view all the answers

Match the following amino acids with their classification:

<p>Glutamic acid = Acidic amino acid Lysine = Basic amino acid Valine = Neutral amino acid Tyrosine = Aromatic amino acid</p> Signup and view all the answers

Which of the following lipids is characterized by having two fatty acids esterified with glycerol?

<p>Diglycerides (B)</p> Signup and view all the answers

Phospholipids are primarily found in cell membranes.

<p>True (A)</p> Signup and view all the answers

What feature distinguishes unsaturated fatty acids from saturated fatty acids?

<p>Presence of one or more double bonds</p> Signup and view all the answers

Which of the following is classified as a primary metabolite?

<p>Amino acids (B)</p> Signup and view all the answers

Primary metabolites have identifiable functions and play known roles in normal physiological processes.

<p>True (A)</p> Signup and view all the answers

The molecular weight range for secondary metabolites is typically less than ______ daltons.

<p>800</p> Signup and view all the answers

What is a characteristic feature of secondary metabolites?

<p>They include compounds such as toxins and pigments. (C)</p> Signup and view all the answers

The ecological importance of secondary metabolites is not currently understood.

<p>False (B)</p> Signup and view all the answers

What types of organic compounds make up the acid insoluble fraction?

<p>Proteins, nucleic acids, polysaccharides, and lipids</p> Signup and view all the answers

What is the primary function of carbonic anhydrase?

<p>To increase the rate of metabolic reactions (C)</p> Signup and view all the answers

Enzymes can catalyze multiple different reactions.

<p>False (B)</p> Signup and view all the answers

What is formed when a substrate binds to the enzyme's active site?

<p>ES complex</p> Signup and view all the answers

Under anaerobic conditions in skeletal muscle, _____ is formed from glucose.

<p>lactic acid</p> Signup and view all the answers

Match the following metabolic end products with their corresponding conditions:

<p>Lactic acid = Anaerobic conditions in skeletal muscle Ethanol = Fermentation in yeast Pyruvic acid = Normal aerobic conditions Glucose = Starting substrate for glycolysis</p> Signup and view all the answers

Which of the following describes the role of an enzyme's active site?

<p>It is the location where substrate binds. (C)</p> Signup and view all the answers

Enzymes change the equilibrium point of a chemical reaction.

<p>False (B)</p> Signup and view all the answers

What happens to the structure of the substrate during the enzymatic reaction?

<p>It is transformed into the product.</p> Signup and view all the answers

What does the energy difference between the stable substrate (S) and the transition state represent?

<p>Activation energy (A)</p> Signup and view all the answers

What is the term for the complex formed when an enzyme binds with its substrate?

<p>Enzyme-substrate complex (ES)</p> Signup and view all the answers

The process by which an enzyme catalyzes the conversion of substrate to product is called ______.

<p>catalysis</p> Signup and view all the answers

What happens to the enzyme after it produces the product?

<p>It remains unchanged (B)</p> Signup and view all the answers

Name one characteristic of a transition state in a chemical reaction.

<p>It is unstable.</p> Signup and view all the answers

The role of enzymes is to stabilize all intermediate states between the substrate and product.

<p>False (B)</p> Signup and view all the answers

What happens to an enzyme when the temperature is increased beyond its optimum level?

<p>The enzyme loses its activity due to denaturation. (B)</p> Signup and view all the answers

Enzymes typically function effectively at any temperature and pH.

<p>False (B)</p> Signup and view all the answers

What is the term used to describe the highest activity point of an enzyme?

<p>optimum temperature and optimum pH</p> Signup and view all the answers

The enzyme returns to its free form after releasing the ______ of the reaction.

<p>products</p> Signup and view all the answers

What happens to the reaction velocity as substrate concentration increases?

<p>It initially rises and then reaches a maximum velocity (Vmax). (B)</p> Signup and view all the answers

All enzymes can function equally well at high substrate concentrations.

<p>False (B)</p> Signup and view all the answers

In what form do enzymes return after a catalytic cycle?

<p>Free enzyme</p> Signup and view all the answers

Which element is found in greater relative abundance in living organisms compared to the earth's crust?

<p>Carbon (A)</p> Signup and view all the answers

The acid-insoluble fraction is where thousands of organic compounds are found.

<p>False (B)</p> Signup and view all the answers

What is the process called that is used to extract organic compounds from living tissue?

<p>Chemical analysis</p> Signup and view all the answers

The method for extracting organic compounds typically involves grinding tissue in __________ acid.

<p>trichloroacetic</p> Signup and view all the answers

What is the main reason why living tissues and the earth's crust consist of the same elements?

<p>They share a common source of elemental formation. (D)</p> Signup and view all the answers

Both living organisms and non-living matter have identical elements, though their quantities differ.

<p>True (A)</p> Signup and view all the answers

What are the two fractions obtained after straining the slurry created from living tissue?

<p>Filtrate and retentate</p> Signup and view all the answers

Flashcards

Amino Acids

Organic compounds with an amino group and an acidic group on the same carbon (alpha-carbon).

Alpha-carbon

The carbon atom in an amino acid that holds both the amino and carboxyl groups.

Fatty Acids

Organic compounds with a carboxyl group attached to a hydrocarbon chain.

Saturated Fatty Acids

Fatty acids with no double bonds between carbon atoms.

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Triglycerides

Lipids formed from glycerol and three fatty acid molecules.

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Phospholipids

Lipids containing a phosphate group, found in cell membranes.

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Zwitterionic form

The internal salt form amino acids take in solution at a certain pH.

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Glycerol

A three-carbon alcohol that combines with fatty acids to form triglycerides.

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Primary Metabolites in Animal Tissues

Common organic compounds found in animal tissues, such as amino acids and sugars.

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Secondary Metabolites

Organic compounds found in plant, fungal, and microbial cells that are not essential for normal physiology.

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Acid Soluble Pool

Collective name for organic compounds of relatively low molecular weight.

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Acid Insoluble Fraction

In cells, this fraction contains high molecular weight compounds like proteins, nucleic acids, polysaccharides, and lipids.

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Biomolecules

Thousands of organic compounds including amino acids and sugars.

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Molecular Weight of Biomolecules

Acid-soluble pool bio molecules have weights from 18 to ~800 daltons; others are above 10,000 daltons.

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Biomacromolecules

Large biological molecules including proteins, nucleic acids, and polysaccharides.

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Importance of secondary metabolites

While not essential for normal physiological processes, they can still be important for ecological interactions and human welfare.

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Protein Structure

The arrangement of amino acids in a protein, determining its shape and function. It exists at four levels: primary, secondary, tertiary, and quaternary.

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Primary Structure

The linear sequence of amino acids in a protein chain, like a string of beads.

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Secondary Structure

The local folding of the protein chain into repeating patterns like α-helices and β-sheets, providing stability.

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Tertiary Structure

The overall three-dimensional shape of a single polypeptide chain, caused by interactions between amino acids.

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Quaternary Structure

The arrangement of multiple polypeptide chains (subunits) to form a functional protein complex.

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Active Site

A specific region on an enzyme where a substrate binds and undergoes a chemical reaction.

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Enzyme

A biological catalyst, usually a protein, that speeds up a chemical reaction without being consumed.

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Ribozyme

A catalytic RNA molecule that acts like an enzyme, capable of carrying out biochemical reactions.

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Starch Structure

Starch molecules form helical (spiral-like) structures, allowing them to hold iodine molecules, resulting in a blue color.

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Cellulose Structure

Cellulose does not form complex helices like starch, therefore it cannot hold iodine molecules.

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Polysaccharide Function

Polysaccharides like cellulose provide structural support, while others like chitin form exoskeletons in arthropods.

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Nucleic Acid Building Block

Nucleotides are the building blocks of nucleic acids, composed of a nitrogenous base, a sugar, and a phosphate group.

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Nitrogenous Bases in Nucleic Acids

There are five nitrogenous bases found in nucleic acids: adenine, guanine, cytosine, thymine, and uracil.

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DNA vs. RNA

DNA contains deoxyribose sugar, while RNA contains ribose sugar.

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What is a Nucleotide?

A nucleotide is composed of a nitrogenous base, a sugar, and a phosphate group.

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Transition State

The highest-energy intermediate state in a chemical reaction, where bonds are breaking and forming.

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Activation Energy

The minimum amount of energy required for a chemical reaction to occur.

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Exothermic Reaction

A reaction that releases energy into the surroundings, resulting in a lower energy state for the product.

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Endothermic Reaction

A reaction that absorbs energy from the surroundings, resulting in a higher energy state for the product.

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Substrate

The molecule that an enzyme acts upon.

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Enzyme-Substrate Complex (ES)

The temporary association formed when an enzyme binds to its substrate.

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Enzyme Inhibition

The process where a chemical (inhibitor) binds to an enzyme and prevents its normal activity.

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Competitive Inhibitor

An inhibitor that resembles the enzyme's substrate and competes for the same binding site.

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Oxidoreductases

Enzymes that catalyze oxidation-reduction reactions, transferring electrons between molecules.

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Transferases

Enzymes that move functional groups (like a methyl or phosphate) from one molecule to another.

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Hydrolases

Enzymes that break down molecules by adding water (hydrolysis).

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Lyases

Enzymes that break bonds without adding water, often forming double bonds.

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Isomerases

Enzymes that rearrange atoms within a molecule to create isomers (molecules with the same formula but different structures).

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What are enzymes classified by?

Enzymes are classified based on the type of reaction they catalyze. There are six main classes: Oxidoreductases, Transferases, Hydrolases, Lyases, Isomerases, and Ligases.

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What is the difference between living and non-living matter in terms of element composition?

While both contain the same elements, living matter has a higher relative abundance of carbon and hydrogen compared to non-living matter.

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What are biomolecules?

Thousands of organic compounds, including amino acids and sugars, found in living organisms.

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What is the difference between biomolecules and biomacromolecules?

Biomolecules are relatively small organic molecules, while biomacromolecules are large polymers made up of many smaller units.

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What is the function of primary metabolites?

They are essential for the normal physiological processes of a cell, like growth and energy production.

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What are secondary metabolites?

Organic compounds found in plants, fungi, and microbes that are not essential for normal physiology, but can have various roles.

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What is the importance of secondary metabolites?

They are important for ecological interactions, like defending against herbivores or attracting pollinators, and for human welfare, like pharmaceuticals and food additives.

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Amino Acid Structure

Amino acids contain an amino group (NH2) and a carboxyl group (COOH) attached to the same carbon atom, the α-carbon. They also have a variable R group.

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Fatty Acid Structure

Fatty acids are long chains of carbon atoms with a carboxyl group at one end. They can be saturated (no double bonds) or unsaturated (one or more double bonds).

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What does 'saturated' mean in fatty acids?

A saturated fatty acid has no double bonds between carbon atoms in its hydrocarbon chain. This makes it more rigid and often solid at room temperature.

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What does 'unsaturated' mean in fatty acids?

An unsaturated fatty acid has one or more double bonds between carbon atoms in its hydrocarbon chain. This makes it more flexible and often liquid at room temperature.

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What is the difference between tertiary and quaternary structure?

Tertiary structure refers to the 3D shape of a single polypeptide chain, while quaternary structure describes the arrangement of multiple polypeptide chains in a protein complex.

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Why is the active site important?

The active site is crucial because it provides a specific location where the substrate binds and the enzyme's catalytic activity takes place.

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What is the difference between an enzyme and a ribozyme?

An enzyme is a protein that acts as a catalyst, while a ribozyme is a catalytic RNA molecule.

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Polymers

Large molecules made up of repeating smaller units called monomers.

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Water

The most abundant chemical compound in living organisms, making up 70-90% of a cell's mass.

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Proteins

Polymers of amino acids linked by peptide bonds. They perform a wide range of functions in cells.

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Nucleic acids

Polymers of nucleotides that carry and express genetic information.

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Enzyme's Power

Enzymes dramatically speed up chemical reactions, often by millions of times. This ability is crucial for life's processes.

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Metabolic Pathway

A series of chemical reactions catalyzed by enzymes, where the product of one step becomes the reactant for the next.

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Anaerobic vs. Aerobic

Metabolic pathways can produce different products based on the presence or absence of oxygen (aerobic vs. anaerobic).

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How do enzymes increase speed?

Enzymes lower the activation energy needed for a reaction to occur, making it proceed faster.

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Enzyme-Substrate Complex

The temporary association formed when an enzyme binds to its substrate.

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What does an enzyme do to activation energy?

Enzymes lower the activation energy of a reaction, making it easier and faster for the reaction to occur.

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What is the nature of an enzyme action?

Enzymes have a specific binding site for their substrate, forming an enzyme-substrate complex (ES). This complex then breaks down into product(s) and the unchanged enzyme.

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What is the key to the reaction?

The formation of the enzyme-substrate (ES) complex is essential for catalysis, as it brings the substrate and enzyme together in the right orientation for the reaction to occur.

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Elemental Analysis

A process that determines the elemental composition of a substance by identifying and quantifying the elements present. For example, in living tissues, this can reveal the relative amounts of elements like carbon, hydrogen, oxygen, and nitrogen.

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Ash Analysis

A specific type of elemental analysis used on biological samples. It involves burning the sample to remove all organic material, leaving behind inorganic elements as ash.

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Inorganic Constituents

Elements and compounds in living organisms that are not carbon-based. Examples include water, salts, minerals like calcium and magnesium.

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Functional Groups

Specific groups of atoms within a molecule responsible for its chemical properties and reactivity. Examples include aldehydes, ketones, and aromatic rings.

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Wet Weight vs. Dry Weight

Wet weight refers to the total weight of a sample including water, while dry weight reflects the weight after all water has been removed.

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Homopolymer

A polymer composed of only one type of monomer repeating multiple times. For example, cellulose is a homopolymer of glucose.

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Heteropolymer

A polymer composed of multiple different types of monomers. Proteins are heteropolymers, as they are made up of 20 different amino acids.

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Essential Amino Acids

Amino acids that our body cannot synthesize and must be obtained through diet.

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Non-Essential Amino Acids

Amino acids that our body can synthesize from other molecules.

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Protein Functions

Proteins perform diverse roles in living organisms, including structural support, catalysis, transport, and defense against infection.

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Collagen

The most abundant protein in the animal world, providing structural support in tissues.

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RuBisCO

The most abundant protein in the biosphere, responsible for carbon fixation in photosynthesis.

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Polysaccharide

A long chain of sugar molecules, often composed of different monosaccharides.

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Carbonic Anhydrase

An enzyme found in the cytoplasm that dramatically speeds up the reaction rate of carbon dioxide and water reacting to form carbonic acid.

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What is the substrate?

The molecule that an enzyme acts upon, undergoing a chemical conversion into a product.

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ES Complex

The temporary association formed when an enzyme binds to its substrate.

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How do enzymes lower activation energy?

Enzymes lower the activation energy needed for a reaction to occur, making it proceed faster.

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What makes the ES complex so important?

It allows for the close proximity and proper orientation of the substrate and enzyme, making the reaction efficient.

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Nucleotide

A nucleotide is the building block of nucleic acids. It is composed of three parts: a nitrogenous base, a sugar, and a phosphate group.

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Nitrogenous Bases

There are five nitrogenous bases found in nucleic acids: adenine, guanine, cytosine, thymine, and uracil.

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Types of Nucleic Acids

Deoxyribonucleic acid (DNA) contains deoxyribose sugar, while ribonucleic acid (RNA) contains ribose sugar.

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How do enzymes affect activation energy?

Enzymes lower the activation energy needed for a reaction to occur, making it easier and faster for the reaction to proceed.

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Co-factor

A non-protein molecule that helps an enzyme function properly.

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Prosthetic group

A tightly bound co-factor that is permanently attached to an enzyme.

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What is the importance of metal ions in enzymes?

Some enzymes need metal ions as co-factors to bind to their substrate and catalyze reactions.

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What happens when a co-factor is removed from an enzyme?

The enzyme loses its catalytic activity, demonstrating the co-factor's crucial role.

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What is the most abundant chemical in living organisms?

Water is the most abundant chemical in living organisms.

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What are the functions of primary metabolites?

They are essential for a cell's normal processes, such as growth and energy production.

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What is the difference in elemental composition between living and non-living matter?

Living matter has higher relative abundance of carbon and hydrogen compared to non-living matter.

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What is the acid-soluble pool?

It's the collection of organic compounds in a living tissue that are soluble in a weak acid.

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What is the acid-insoluble fraction?

It's the collection of large organic molecules in a living tissue that don't dissolve in a weak acid.

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What's elemental analysis?

A process that determines the elemental composition of a substance, identifying and quantifying the elements present.

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Adenine

A purine base found in both DNA and RNA. It pairs with thymine in DNA and with uracil in RNA.

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Guanine

A purine base found in both DNA and RNA. It pairs with cytosine.

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Cytosine

A pyrimidine base found in both DNA and RNA. It pairs with guanine.

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Thymine

A pyrimidine base found only in DNA. It pairs with adenine.

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Uracil

A pyrimidine base found only in RNA. It pairs with adenine.

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Primary Metabolites

Primary metabolites are essential organic compounds found in all living organisms that are directly involved in normal physiological processes like growth, energy production, and reproduction. Examples include carbohydrates, amino acids, and lipids.

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What are polysaccharides?

Long chains of sugar molecules (monosaccharides) linked together. Examples include starch, cellulose, and chitin.

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What is the function of starch?

Starch is a storage polysaccharide in plants. It forms helical structures that can hold iodine, giving a blue color.

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What is different about cellulose compared to starch?

Cellulose does not form complex helices like starch. It is the main component of plant cell walls.

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What makes up the cell wall of plants?

Plant cell walls are primarily made of cellulose, a complex polysaccharide.

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What is chitin?

A complex polysaccharide that forms the exoskeletons of arthropods (like insects and crabs).

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What are the differences between DNA and RNA?

DNA contains deoxyribose sugar and the base thymine, while RNA contains ribose sugar and the base uracil.

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What are the functions of nucleic acids?

Nucleic Acids (DNA and RNA) are responsible for storing and expressing genetic information in living organisms.

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N-Terminal Amino Acid

The first amino acid in a protein chain, having a free amino group.

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C-Terminal Amino Acid

The last amino acid in a protein chain, having a free carboxyl group.

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Right-Handed Helix

The specific type of helix observed in protein secondary structures. It’s a spiral with a clockwise rotation when viewed from the top.

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What are the four levels of protein structure?

The four levels of protein structure are primary, secondary, tertiary, and quaternary. Each level contributes to the protein's unique 3D shape and function.

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What is the difference between N-terminal and C-terminal amino acids?

The N-terminal amino acid has a free amino group at the beginning of the chain, while the C-terminal amino acid has a free carboxyl group at the end of the chain.

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What determines the function of a protein?

The specific three-dimensional shape of a protein, determined by its amino acid sequence and folding, dictates its function. It's like how a key's shape fits a specific lock, each protein has a unique shape for its job.

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Subunits

Individual folded polypeptide chains that come together to form a protein with quaternary structure. Think of them as the building blocks of a complex structure.

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How are enzymes classified?

Enzymes are classified based on the type of reaction they catalyze. There are six main classes: Oxidoreductases, Transferases, Hydrolases, Lyases, Isomerases, and Ligases.

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What makes enzyme action possible?

The formation of the enzyme-substrate (ES) complex is essential for catalysis, as it brings the substrate and enzyme together in the right orientation for the reaction to occur.

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What is the key to enzyme action?

The formation of the enzyme-substrate (ES) complex is essential for catalysis, as it brings the substrate and enzyme together in the right orientation for the reaction to occur.

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What are α-amino acids?

Organic compounds containing an amino group and an acidic group attached to the same carbon atom (α-carbon). They are the building blocks of proteins.

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What are the four substituents in an amino acid?

Hydrogen, carboxyl group, amino group, and a variable 'R' group, which determines the amino acid's specific properties.

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What are the R groups in amino acids?

They are the variable groups attached to the α-carbon and define the specific properties of each amino acid.

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What are fatty acids?

Organic compounds with a carboxyl group attached to a hydrocarbon chain. They are major components of lipids.

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Difference between saturated and unsaturated fatty acids?

Saturated fatty acids have no double bonds between carbon atoms. Unsaturated fatty acids have at least one double bond.

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What is glycerol?

A simple lipid that is a three-carbon alcohol. It combines with fatty acids to form triglycerides.

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What are triglycerides?

Lipids formed from glycerol and three fatty acid molecules. They are fats and oils, depending on their melting point.

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What are phospholipids?

Lipids containing a phosphate group. They are major components of cell membranes.

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What are primary metabolites?

Organic compounds essential for normal physiological processes within a cell, such as growth and energy production.

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Reducing End

The end of a polysaccharide chain where the last sugar has a free anomeric carbon, making it reactive.

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Induced Fit Model

The binding of the substrate to the enzyme induces a change in the enzyme's shape, making it fit more tightly around the substrate.

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Optimum Temperature and pH

Each enzyme has a specific temperature and pH at which it exhibits maximum activity.

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Factors Affecting Enzyme Activity

Enzyme activity can be influenced by temperature, pH, substrate concentration, and the presence of specific chemicals.

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Enzyme Catalysis

Enzymes significantly speed up chemical reactions by lowering the activation energy needed for the reaction to occur.

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Vmax

The maximum velocity of an enzymatic reaction, reached when all enzyme molecules are saturated with substrate.

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Metal Ions as Cofactors

Metal ions can act as co-factors by forming bonds with the enzyme and the substrate.

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Importance of Cofactors

Cofactors are essential for enzyme activity, and their removal leads to loss of catalytic function.

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Elemental Composition of Living Matter

Living matter contains the same elements as non-living matter, but with a higher relative abundance of carbon and hydrogen.

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Why are secondary metabolites important?

They are important for ecological interactions and human welfare.

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What is a Protein?

A heteropolymer made up of amino acid monomers linked together by peptide bonds. They perform various functions in the body.

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What is the purpose of secondary metabolites?

Secondary metabolites are important for ecological interactions – defense, communication, and attracting pollinators. They also have valuable uses for humans, like pharmaceuticals and food additives.

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What's the difference between primary and secondary metabolites?

Primary metabolites are essential for basic life functions, while secondary metabolites have more specialized roles, often for interactions with the environment.

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How are biomolecules classified?

Biomolecules are classified based on their solubility in weak acids. The acid-soluble pool contains small molecules, while the acid-insoluble fraction contains large biomacromolecules.

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Enzyme Stability

Enzymes, unlike inorganic catalysts, are sensitive to high temperatures. However, enzymes from thermophilic organisms (living in hot environments) can withstand high temperatures and maintain their activity.

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Chemical Reaction

A chemical reaction involves the breaking and forming of chemical bonds, resulting in the creation of new substances with different properties. Physical changes only alter the form of a substance without changing its chemical composition.

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Rate of Reaction

The rate of a reaction describes how quickly a reactant is transformed into a product, measured as the amount of product formed per unit time.

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Catalyzed vs. Uncatalyzed

Catalysts, including enzymes, dramatically accelerate the rate of a chemical reaction. Without a catalyst, the reaction would happen very slowly.

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Thermal Stability

The ability of a molecule, like an enzyme, to retain its structure and functionality even at high temperatures.

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What is a catalyst?

A catalyst, such as an enzyme, speeds up a chemical reaction without being consumed in the process. It lowers the activation energy required for the reaction to occur.

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How do enzymes work?

Enzymes provide a specific site (active site) for their substrate to bind. This interaction initiates a chemical transformation, converting the substrate into a product.

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What happens to enzyme activity at optimal temperature?

Enzyme activity is at its peak, with the highest reaction rate due to maximum molecular collisions.

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What happens to enzyme activity at high temperatures?

Enzyme activity decreases rapidly due to denaturation, where the enzyme's structure unfolds and loses its function.

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What happens to enzyme activity at low temperatures?

Enzyme activity slows down because there are fewer molecular collisions, but the enzyme remains intact and can regain activity upon warming.

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What is the effect of increasing substrate concentration on enzyme activity?

As substrate concentration increases, enzyme activity rises until it reaches a maximum velocity (Vmax) where all enzyme active sites are saturated.

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What are factors that can affect enzyme activity?

Temperature, pH, substrate concentration, and the presence of specific chemicals (inhibitors or activators) can all affect enzyme activity.

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What is the difference between saturated and unsaturated fatty acids?

Saturated fatty acids have no double bonds between carbon atoms in their chain. Unsaturated fatty acids have one or more double bonds.

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What is the zwitterionic form?

The form an amino acid takes in solution when it has both a positive and a negative charge, due to the ionization of its amino and carboxyl groups.

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What is the difference between DNA and RNA?

DNA contains deoxyribose sugar and the base thymine, while RNA contains ribose sugar and the base uracil.

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What is the active site of an enzyme?

A specific region on an enzyme where a substrate binds and undergoes a chemical reaction.

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How does an enzyme accelerate a reaction?

Enzymes lower the activation energy needed for a reaction to occur, allowing it to proceed faster.

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What is the importance of the active site?

The active site is crucial because it provides a specific location where the substrate binds and the enzyme's catalytic activity occurs.

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What happens to enzyme activity with a competitive inhibitor?

The enzyme's activity decreases as the inhibitor competes with the substrate for the binding site.

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Physical Change

Alterations in the physical properties of a substance, such as shape, without changing its chemical composition.

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Catalysts

Substances that speed up chemical reactions without being consumed in the process.

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Enzymes as Catalysts

Biological catalysts, typically proteins, that accelerate specific biochemical reactions in living organisms.

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Uncatalyzed vs. Catalyzed

Catalyzed reactions proceed at significantly faster rates than uncatalyzed reactions. Enzymes, as catalysts, greatly enhance the speed of biological reactions.

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What is catalysis?

The process of speeding up a chemical reaction using a catalyst, like an enzyme. Catalysts are not consumed in the reaction.

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How do enzymes make reactions easier?

Enzymes lower the activation energy barrier, making it easier for the reaction to start. This is achieved by providing an alternative pathway with a lower energy requirement.

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What are the five nitrogenous bases in DNA and RNA?

Adenine, guanine, cytosine, thymine, and uracil. Adenine and guanine are purines, while cytosine, thymine, and uracil are pyrimidines.

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What is the role of carbohydrates in living organisms?

Carbohydrates are a primary source of energy for living organisms. They also provide structural support and act as signaling molecules.

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Molecular Weight Range

Acid-soluble pool compounds have molecular weights from 18 to around 800 daltons, while the acid insoluble fraction compounds have molecular weights above 10,000 daltons.

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What are the 4 main types of biomacromolecules?

The four main types of biomacromolecules are proteins, nucleic acids, polysaccharides, and lipids.

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Enzyme-Product Complex

The temporary association formed when an enzyme binds to its products after the reaction is complete.

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Optimum Temperature

The temperature at which an enzyme shows its maximum activity.

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Optimum pH

The pH at which an enzyme shows its maximum activity.

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Denaturation

Loss of an enzyme's structure and function due to exposure to extreme temperatures or pH changes.

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Vmax (Maximum Velocity)

The highest rate of reaction an enzyme can achieve, beyond which increasing substrate concentration has no effect.

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Collagen: Most Abundant Animal Protein

The most abundant protein found in the animal kingdom, providing structural support in connective tissues.

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RuBisCO: Most Abundant Protein in the Biosphere

The most abundant protein on earth, key enzyme in carbon fixation during photosynthesis.

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Polysaccharide: Chains of Sugars

Long chains of sugar molecules, often composed of different monosaccharides.

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Cellulose: Homopolymer of Glucose

A polysaccharide composed solely of glucose monomers, providing structural support in plants.

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Enzyme Catalysts vs. Inorganic Catalysts

Enzymes are biological catalysts that function efficiently at moderate temperatures, while inorganic catalysts often require high temperatures and pressures.

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Thermophilic Enzymes

Enzymes isolated from organisms that thrive in extremely hot environments, like hot springs, are remarkably stable at high temperatures.

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Chemical Reaction Definition

A chemical reaction involves breaking and forming new bonds between atoms to create different molecules.

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Effect of Temperature on Reaction Rate

Generally, increasing temperature speeds up a chemical reaction, while decreasing temperature slows it down.

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Catalysts and Reaction Rate

Catalysts, including enzymes, significantly increase the speed of chemical reactions.

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Enzyme Catalysis Example

Carbonic anhydrase, an enzyme, dramatically speeds up the reaction between carbon dioxide and water to produce carbonic acid.

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Uncatalyzed vs. Enzyme-Catalyzed Reactions

The same chemical reaction can proceed at vastly different rates depending on whether an enzyme is involved.

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What are Nucleic Acids?

Nucleic acids are polymers of nucleotides that carry and express genetic information.

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What is an enzyme's role in a reaction?

Enzymes speed up chemical reactions without being consumed. They do this by lowering the activation energy needed for the reaction to occur.

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What is the Quaternary structure?

The arrangement of multiple polypeptide chains (subunits) in a protein complex.

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How do different Levels of protein structure relate?

Primary structure is the amino acid sequence, which determines secondary structure (local folds), tertiary structure (overall 3D shape), and finally, quaternary structure (arrangement of multiple subunits).

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How do enzymes speed up reactions?

Enzymes lower the activation energy required for a reaction to occur. This allows the reaction to proceed faster without changing the overall energy difference between reactants and products.

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What is the importance of the ES complex?

It allows for the close proximity and proper orientation of the substrate and enzyme, making the reaction efficient.

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Enzyme Activity

The rate at which an enzyme catalyzes a specific reaction. It's influenced by factors like temperature, pH, substrate concentration, and the presence of inhibitors or activators.

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Substrate Concentration

The amount of substrate present in a reaction mixture. Increasing substrate concentration initially boosts reaction speed, but plateaus at a maximum velocity (Vmax).

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Transition State Structure

A new, unstable structure formed when the substrate binds to the active site of an enzyme.

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What are the R-groups?

The variable group attached to the α-carbon in amino acids. The R-group determines the specific properties of each amino acid.

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Saturated vs. Unsaturated

Saturated fatty acids have no double bonds between carbon atoms, while unsaturated fatty acids have one or more double bonds. This affects their flexibility and melting point.

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What is the importance of the 'acid-soluble pool'?

This group contains organic compounds of low molecular weight, essential for cell functioning. They include sugars, amino acids, nucleotides, and other small molecules.

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What is the difference between acid-soluble pool and acid-insoluble fraction?

The acid-soluble pool contains small organic molecules, while the acid-insoluble fraction contains large polymers.

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Why are biomacromolecules important?

They provide structural support, store and transmit genetic information, catalyze reactions, and perform numerous other roles essential for life.

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What is the effect of enzymes on activation energy?

Enzymes reduce the activation energy needed for a reaction to proceed. This makes the reaction happen faster and at lower temperatures.

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What is the key to enzyme catalysis?

The formation of the ES complex is essential for catalysis. This is because it brings the substrate and enzyme into close proximity and correct orientation for the reaction to take place.

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What is the difference between exothermic and endothermic reactions?

Exothermic reactions release energy, while endothermic reactions absorb energy. Exothermic reactions feel hot, while endothermic reactions feel cold.

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How does temperature affect enzyme activity?

Each enzyme has an optimal temperature range for maximum activity. Below this range, activity decreases, and above it, activity decreases due to denaturation.

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How does pH affect enzyme activity?

Enzymes have optimal pH ranges for maximum activity. Deviating from the optimal pH can affect the enzyme's shape and reduce activity.

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What is denaturation of an enzyme?

Denaturation is the process where an enzyme's three-dimensional structure is disrupted, rendering it inactive. This can happen due to extreme temperature or pH changes.

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Study Notes

Biomolecules

  • Living organisms are composed of various elements and compounds
  • Elemental analysis of plant/animal tissue or microbial paste reveals similar elements (C, H, O, etc.) to those found in Earth's crust.
  • However, the relative abundance of C and H is higher in living organisms.

Chemical Composition Analysis

  • Chemical analysis of living tissue (e.g., vegetable, liver) is performed, typically by grinding the tissue in trichloroacetic acid.
  • This process separates the tissue into two fractions: acid-soluble and acid-insoluble.
  • Scientists have identified thousands of organic compounds within the acid-soluble fraction.

Lipids

  • Generally water insoluble
  • Consists of a carboxyl group attached to an R group (e.g., methyl, ethyl, CH₂ groups)
  • Classified as saturated (no double bonds) or unsaturated (one or more double bonds) based on the number of carbon atoms in the R group.
  • Examples include glycerol, monoglycerides, diglycerides, triglycerides (fats/oils), phospholipids and diverse lipids in neural tissues.

Carbohydrates

  • Polysaccharides are long sugar chains (e.g. cellulose, glycogen, inulin).
  • Contain different monosaccharides as building blocks (e.g., glucose).
  • In some cases, the chain has a branching structure.

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Biomolecules PDF

Description

Explore the essential biomolecules that compose living organisms, focusing on their elemental analysis and chemical composition. This quiz covers the classification of lipids and their characteristics, including saturated and unsaturated types. Test your knowledge about the different organic compounds found in living tissues.

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