Biomedical Engineering: Kinetics in Cells
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Questions and Answers

What is the main purpose of controlling the fermentor conditions?

  • To maintain a constant level of ambient conditions (correct)
  • To increase the initial substrate concentration
  • To validate the Monod kinetic model
  • To ensure high concentrations of all components

    • What type of coordinates are used in the graphical representation of biomass growth?

  • Linear coordinates
  • Arithmetic coordinates
  • Logarithmic coordinates (correct)
  • Exponential coordinates

    • Which model is used to describe the slow growth of cells at low concentrations?

  • Modified logistic model
  • Logistic model
  • Leudeking-Piret model
  • Monod equation (correct)

    • What is the unit of the specific growth rate μ in the Monod kinetic model?

    <p>h-1</p> Signup and view all the answers

    How are microorganisms classified based on their temperature requirements?

    <p>Based on their temperature requirements, such as psychrofiles, mesofiles, and thermofiles</p> Signup and view all the answers

    What is the effect of initial substrate concentration on the growth kinetics of cells?

    <p>It is accounted for in certain kinetic models, such as the Monod model</p> Signup and view all the answers

    What is the primary purpose of a recipient in cell growth?

    <p>To control parameters such as oxygen and pH</p> Signup and view all the answers

    What is the unit of measurement for cellular density?

    <p>Grams per liter</p> Signup and view all the answers

    Which method involves counting cells in a suspension under a microscope?

    <p>Using a counting chamber (Bürker's chamber)</p> Signup and view all the answers

    What is the purpose of drying cells at 105°C?

    <p>To express the cell concentration as a mass per volume</p> Signup and view all the answers

    What is an indirect method for measuring cell concentration?

    <p>Measuring the concentration of RNA</p> Signup and view all the answers

    What is the common unit of measurement for cell concentration?

    <p>Grams per cubic decimeter</p> Signup and view all the answers

    What is the term used to describe the speed of a chemical reaction?

    <p>Reaction rate</p> Signup and view all the answers

    What is the significance of controlling the speed of a chemical reaction?

    <p>It is important for biomedical engineering applications</p> Signup and view all the answers

    What is studied in single molecule experiments in living cells?

    <p>Kinetic information from single molecules</p> Signup and view all the answers

    What can be followed in time after a perturbation at t0?

    <p>The fraction of molecules in various states of binding or diffusion</p> Signup and view all the answers

    What is shown in Figure 3 of the text?

    <p>Contact angle images of APAPJ treated polystyrene samples</p> Signup and view all the answers

    What is the application of Atmospheric Pressure Argon Plasma Jet (APAPJ) in?

    <p>Biomedical science and engineering</p> Signup and view all the answers

    What is the effect of doubling the concentration of a reactant on the rate of a zero-order reaction?

    <p>The rate of the reaction remains the same</p> Signup and view all the answers

    In a first-order reaction, what is the effect of doubling the concentration of the reactant on the rate of the reaction?

    <p>The rate of the reaction is doubled</p> Signup and view all the answers

    What is the effect of doubling the concentration of a reactant on the rate of a second-order reaction?

    <p>The rate of the reaction is quadrupled</p> Signup and view all the answers

    How is the rate law determined?

    <p>Experimentally, using the method of initial rates</p> Signup and view all the answers

    What is the purpose of changing the initial concentration of one reactant at a time in an experiment?

    <p>To determine the effect of each reactant's concentration on the rate</p> Signup and view all the answers

    What is the purpose of an integrated rate law?

    <p>To relate the rate of the reaction to the concentration of the reactant</p> Signup and view all the answers

    What is the approximate solubility of oxygen in water at 25 °C and a pressure of 101 kPa?

    <p>8 ppm</p> Signup and view all the answers

    What is the critical concentration of dissolved oxygen in a solution, relative to the saturated concentration of O2?

    <p>10%</p> Signup and view all the answers

    What is a characteristic of cell growth that mathematical models should take into account?

    <p>The growth is generally unbalanced, with changes in biomass composition</p> Signup and view all the answers

    Why are simplified models of cell growth often used, despite being less precise?

    <p>They are easier to use and more practical for operations</p> Signup and view all the answers

    What is a factor that can inhibit cell growth, according to the parameters for kinetics?

    <p>All of the above</p> Signup and view all the answers

    What is the unit of measurement for the solubility of oxygen in water, according to the text?

    <p>ppm</p> Signup and view all the answers

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