30 Questions
What is the main purpose of controlling the fermentor conditions?
To maintain a constant level of ambient conditions
What type of coordinates are used in the graphical representation of biomass growth?
Logarithmic coordinates
Which model is used to describe the slow growth of cells at low concentrations?
Monod equation
What is the unit of the specific growth rate μ in the Monod kinetic model?
h-1
How are microorganisms classified based on their temperature requirements?
Based on their temperature requirements, such as psychrofiles, mesofiles, and thermofiles
What is the effect of initial substrate concentration on the growth kinetics of cells?
It is accounted for in certain kinetic models, such as the Monod model
What is the primary purpose of a recipient in cell growth?
To control parameters such as oxygen and pH
What is the unit of measurement for cellular density?
Grams per liter
Which method involves counting cells in a suspension under a microscope?
Using a counting chamber (Bürker's chamber)
What is the purpose of drying cells at 105°C?
To express the cell concentration as a mass per volume
What is an indirect method for measuring cell concentration?
Measuring the concentration of RNA
What is the common unit of measurement for cell concentration?
Grams per cubic decimeter
What is the term used to describe the speed of a chemical reaction?
Reaction rate
What is the significance of controlling the speed of a chemical reaction?
It is important for biomedical engineering applications
What is studied in single molecule experiments in living cells?
Kinetic information from single molecules
What can be followed in time after a perturbation at t0?
The fraction of molecules in various states of binding or diffusion
What is shown in Figure 3 of the text?
Contact angle images of APAPJ treated polystyrene samples
What is the application of Atmospheric Pressure Argon Plasma Jet (APAPJ) in?
Biomedical science and engineering
What is the effect of doubling the concentration of a reactant on the rate of a zero-order reaction?
The rate of the reaction remains the same
In a first-order reaction, what is the effect of doubling the concentration of the reactant on the rate of the reaction?
The rate of the reaction is doubled
What is the effect of doubling the concentration of a reactant on the rate of a second-order reaction?
The rate of the reaction is quadrupled
How is the rate law determined?
Experimentally, using the method of initial rates
What is the purpose of changing the initial concentration of one reactant at a time in an experiment?
To determine the effect of each reactant's concentration on the rate
What is the purpose of an integrated rate law?
To relate the rate of the reaction to the concentration of the reactant
What is the approximate solubility of oxygen in water at 25 °C and a pressure of 101 kPa?
8 ppm
What is the critical concentration of dissolved oxygen in a solution, relative to the saturated concentration of O2?
10%
What is a characteristic of cell growth that mathematical models should take into account?
The growth is generally unbalanced, with changes in biomass composition
Why are simplified models of cell growth often used, despite being less precise?
They are easier to use and more practical for operations
What is a factor that can inhibit cell growth, according to the parameters for kinetics?
All of the above
What is the unit of measurement for the solubility of oxygen in water, according to the text?
ppm
Test your knowledge of kinetics in cells, including counting methods, spectrophotometric analysis, and single molecule experiments in living cells. Learn about the principles of biomedical engineering and its applications in cellular engineering.
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