Biology Quiz on Fermentation and Enzymes

Questions and Answers

Under what environmental conditions do yeast carry out fermentation?

Low pH

All of the choices are correct (correct)

High temperature

Absence of oxygen

High osmotic pressure

The active site is located on the substrate.

False

An increase in the concentration of substrate will result in:

An increase in velocity of the reaction, until all of the enzyme is bound by substrate (correct)

A decrease in velocity because of oversaturation

Concentration of substrate does not affect properties of the reaction

A steady and continued increase in the velocity of the reaction

A steady and continued decrease in the velocity of the reaction

Enzyme activity can be determined in the following ways:

By measuring how much of the substrate is consumed Signup and view all the answers

The optimum condition for an enzyme is:

Is where it works best Signup and view all the answers

Enzymes speed up reactions by:

Lowering activation energy Signup and view all the answers

Catalase activity can be determined by:

Measuring the formation of oxygen gas Signup and view all the answers

In an enzyme-controlled reaction, a substrate is the same as:

Reactant Signup and view all the answers

In a catalase controlled experiment, the negative control can be shown by:

A mixture of hydrogen peroxide and water Signup and view all the answers

In a catalase controlled experiment, the specificity of catalase can be shown by comparing:

A mixture of water and catalase Signup and view all the answers

Polysaccharides are easier to ferment as compared to monosaccharides.

False Signup and view all the answers

The products of fermentation by yeast are:

Carbon dioxide (CO2) and ethanol (C2H6O) + 2ATP Signup and view all the answers

Yeast can produce 2 ATP per molecule of glucose when using fermentation.

True Signup and view all the answers

Study Notes

Fermentation Conditions

Yeasts perform fermentation in the absence of oxygen, under high temperature, low pH, and high osmotic pressure.
All listed conditions are correct for yeast fermentation.

Enzyme Active Site

The active site of an enzyme is located on the enzyme itself, not on the substrate.
True or false: The statement regarding the active site is false.

Substrate Concentration and Reaction Velocity

Increasing substrate concentration boosts reaction velocity until all enzyme active sites are occupied.
Once saturation occurs, adding more substrate does not increase the reaction rate.

Measuring Enzyme Activity

Enzyme activity can be assessed by determining:
- Amount of substrate consumed
- Amount of product produced
Equivalence of substrate and product can also indicate activity.

Optimum Conditions for Enzymes

Optimum conditions for enzyme function refer to the environment where an enzyme operates best, which can vary (e.g., 60°C, room temperature).

Enzyme Function

Enzymes enhance reaction rates by lowering activation energy required for reactions to proceed.
They do not raise activation energy or produce heat to facilitate reactions.

Catalase Activity Measurement

Catalase activity is measured by observing the formation of oxygen gas during the breakdown of hydrogen peroxide.
Other methods, such as measuring catalase amounts or ratios, are less direct for assessing activity.

Substrates and Reactants

In enzyme-controlled reactions, substrates are equivalent to reactants.
They are not products, enzymes, or inhibitors.

Controlled Experiments with Catalase

Negative controls in catalase experiments may involve mixtures of hydrogen peroxide and water, or other combinations that exclude catalase.
Specificity of catalase can be demonstrated by comparing its activity in different mixtures.

Fermentation Products

The fermentation process in yeasts produces carbon dioxide (CO2) and ethanol (C2H6O), yielding 2 ATP molecules.
Alternative outcomes involving different ATP yields or products are incorrect.

Yeast Fermentation Efficiency

Yeast can generate 2 ATP per glucose molecule during fermentation.
This fact confirms the efficiency of yeast in anaerobic conditions.

Description

Test your knowledge on fermentation processes and the role of enzymes in biochemical reactions. This quiz covers key concepts such as the conditions necessary for fermentation, as well as the relationship between enzymes and substrates. Challenge yourself with true or false questions and multiple-choice scenarios.