Biology of Sterilization
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Questions and Answers

Which type of microorganisms are used as biological indicators (BIs)?

  • Gram-negative bacteria
  • Spore-forming microbes in an active state
  • Non-spore-forming microbes
  • Gram-positive bacteria (correct)
  • Why are spore-forming microbes used as BIs instead of non-spore-forming microbes?

  • Non-spore-forming microbes are more resistant to sterilization
  • Spore-forming microbes represent the actual bioburden on items (correct)
  • Non-spore-forming microbes are more prevalent in the environment
  • Spore-forming microbes are easier to kill during sterilization
  • What is the purpose of a biological indicator (BI)?

  • To test the effectiveness of a sterilization process (correct)
  • To identify the type of microorganisms present
  • To determine the bioburden on items
  • To clean and disinfect items
  • What is the resistance of a biological indicator (BI) during sterilization?

    <p>It is highly resistant</p> Signup and view all the answers

    Why are spore-forming microorganisms well characterized and selected for biological indicators (BIs)?

    <p>To ensure their effectiveness as BIs</p> Signup and view all the answers

    Which type of microorganisms are used as biological indicators (BIs)?

    <p>Spore-forming microorganisms</p> Signup and view all the answers

    What is the purpose of using biological indicators (BIs) in sterilization processes?

    <p>To validate the effectiveness of sterilization processes</p> Signup and view all the answers

    Why are spore-forming microbes preferred as biological indicators (BIs) over non-spore-forming microbes?

    <p>Spore-forming microbes are easier to kill during sterilization</p> Signup and view all the answers

    What does the ability of a biological indicator (BI) to mimic 'worst-case' bioburden scenarios validate?

    <p>The effectiveness of sterilization processes</p> Signup and view all the answers

    Why are spore-forming microorganisms considered a minimal subset of the actual bioburden on items to be sterilized?

    <p>Spore-forming microorganisms are more common in worst-case scenarios</p> Signup and view all the answers

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