5 Questions
List three methods of sterilization and provide an example for each.
Three methods of sterilization are red heat (e.g. holding object in flame till red hot), flaming (e.g. passing object through flame without redness), and hot air oven (e.g. sterilizing glass wares at 160-180°C for 1.5-2 hours).
What is the sterilization time and temperature for autoclave sterilization?
Autoclave sterilization is conducted at 121°C for 20 minutes at 15 lb pressure.
Explain the function of a microscope.
The function of a microscope includes magnification, resolution (sharpness), and contrast.
How do gamma rays affect microorganisms and which bacteriological media are not affected by autoclaving?
Gamma rays damage the nucleic acid of microorganisms (bactericidal). The bacteriological media not affected by autoclaving are litmus milk media, sugar media, and gelatin media.
Give examples of items that cannot withstand heat sterilization methods and explain why.
Items such as plastic, syringes, gloves, catheters, cat gut, and rubber tubes cannot withstand heat sterilization methods because they would be damaged or decomposed by the high temperatures.
Test your knowledge with Dr. Hassnaa Hiessen's revision quiz covering topics like microscope functions and methods of sterilization. Challenge yourself with questions on magnification, resolution, contrast, and various sterilization methods including red heat, flaming, and hot air oven.
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