Questions and Answers
What is a colony?
What does a mixed culture contain?
More than one type of organism
Define a pure culture.
A population of cells or multicellular organisms growing in the absence of other species or types.
Sterile means to eliminate all forms of life.
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Which of the following is a characteristic of Eosin Methylene Blue?
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What is the purpose of transfers in microbiology?
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What are techniques in microbiology used for?
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What is the function of inoculating loops?
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What does a pipette do?
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What is the forcepts technique used for?
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Study Notes
Colony
- Defined as a mass of microorganisms derived from a single mother cell.
- Constitutes a clone of bacteria that are genetically identical.
Mixed Culture
- Contains multiple types of organisms in a sterile medium like agar.
- Can include various species such as viruses, bacteria, and parasites, which may interact or compete for resources.
Pure Culture
- Also known as axenic culture, consisting of one type of organism or cells.
- Originates from a single cell or organism, resulting in genetic clones.
Sterilization
- Process that eliminates all forms of life and biological agents.
- Targets fungi, bacteria, viruses, spores, prions, and unicellular eukaryotic organisms.
Media
- Eosin methylene blue agar is toxic to Gram-positive bacteria.
- Yeast extract and malt extract (YM) agar has a low pH to inhibit bacterial growth.
- MacConkey agar is selective for Gram-negative bacteria.
- Hektoen enteric agar specifically targets Gram-negative bacteria.
Transfers
- Refers to moving living microbes from one location to another.
- Ensures no contamination occurs with the culture, sterile medium, or surroundings.
Techniques
- Methods used for studying microbes, including bacteria, fungi, and protists.
- Techniques include surveying, culturing, staining, identifying, and manipulating microbes.
Inoculating Loops
- Simple tool used primarily by microbiologists to collect inoculum from cultures.
- Facilitates the cultivation of microbes on plates through inoculum transfer for streaking.
Pipettes
- Instruments designed for transporting a measured volume of liquid.
- Commonly used as media dispensers in microbial handling.
Forceps Technique
- Technique used in microbiology for handling and manipulating samples or cultures.
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Description
This quiz covers essential microbiology concepts related to colony formation, mixed and pure cultures, and the various methods of sterilization. Additionally, it explores specific types of media used for bacterial growth and their selective properties. Test your knowledge on these fundamental aspects of microbiological practices.
