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Questions and Answers
What is the primary purpose of using a wet mount slide?
How is total magnification calculated when using a compound light microscope?
Which type of medium is used to suspend a sample during the observation of human cheek cells?
What component of a cell is primarily observed when using iodine in a wet mount?
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What should be done before attending the lab quiz on the microscope?
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What is the purpose of using a compound light microscope?
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Which of the following best describes prepared slides?
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What does Field of View (FOV) refer to in microscopy?
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What is the recommended protocol for preparing a wet mount slide?
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What should each lab partner do during the microscope lab?
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What is considered cheating during the microscope lab?
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What component of the microscope is responsible for magnification?
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Where should broken glass be disposed of during the lab?
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Study Notes
Microscope Overview
- A compound light microscope is used for viewing small cells with greater detail than the naked eye can provide.
- Prepared slides feature samples that are permanently attached, ensuring safety for both the user and the microscope while lasting for many years.
Microscope Components
- Total Magnification is calculated by multiplying the magnification of the objective lens by that of the eyepiece.
- The Field of View (FOV) refers to the circle of light visible through the microscope, encompassing the specimen being observed.
Lab Protocols
- Work collaboratively to prepare and focus different specimen slides, rotating among lab partners to observe and draw from each microscope.
- Original drawings and measurements must be made by each student to avoid plagiarism.
Prepared Slides
- Letter "e" Slide: A basic prepared slide exercise.
- Spirogyra: Identify cell wall, nucleus, and chloroplasts.
- Paramecium: Locate cytoplasm, vacuoles, and nucleus.
- Human Blood: Examine nucleus, white blood cells (WBCs), and red blood cells (RBCs).
- Sickle Cell Anemia: A specific prepared slide for studying abnormalities in blood cells.
- Frog/Amphibian Blood: Differentiate between white and red blood cells.
Wet Mount Technique
- Protocol for creating a wet mount includes drying the slide and placing a drop of water or stain, followed by adding the specimen and covering with a coverslip.
- Dispose of broken glass properly; know the designated area.
Common Wet Mount Samples
- Elodea Leaf: Use water to suspend.
- Human Cheek Cells: Use methylene blue for staining.
- Onion Cells: Use iodine for staining.
- Pond Water: Various microorganisms can be observed.
Key Learning Objectives
- Master metric conversions; familiarize with the conversions table.
- Define and understand the function of the compound light microscope's main components.
- Calculate total magnification and identify various cell types, their parts, and functions.
- Understand the process and purpose of making a wet mount, along with the necessity of using a medium.
Upcoming Reminders
- Review syllabus and engage with instructor via Canvas for any questions.
- Prepare for the upcoming lab on "Cell Processes."
- Upcoming lab quiz covering microscope topics; bring the specified Scantron form, a pencil, and optionally, a basic calculator.
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Description
This quiz covers the essentials of using a compound light microscope, including its components and function. It also addresses laboratory protocols for observing prepared slides. Test your knowledge of microscopy terminology and techniques in this engaging quiz!
