Biology Microscope Overview and Lab Protocols

Microscope Overview

• A compound light microscope is used for viewing small cells with greater detail than the naked eye can provide.
• Prepared slides feature samples that are permanently attached, ensuring safety for both the user and the microscope while lasting for many years.

Microscope Components

• Total Magnification is calculated by multiplying the magnification of the objective lens by that of the eyepiece.
• The Field of View (FOV) refers to the circle of light visible through the microscope, encompassing the specimen being observed.

Lab Protocols

• Work collaboratively to prepare and focus different specimen slides, rotating among lab partners to observe and draw from each microscope.
• Original drawings and measurements must be made by each student to avoid plagiarism.

Prepared Slides

• Letter "e" Slide: A basic prepared slide exercise.
• Spirogyra: Identify cell wall, nucleus, and chloroplasts.
• Paramecium: Locate cytoplasm, vacuoles, and nucleus.
• Human Blood: Examine nucleus, white blood cells (WBCs), and red blood cells (RBCs).
• Sickle Cell Anemia: A specific prepared slide for studying abnormalities in blood cells.
• Frog/Amphibian Blood: Differentiate between white and red blood cells.

Wet Mount Technique

• Protocol for creating a wet mount includes drying the slide and placing a drop of water or stain, followed by adding the specimen and covering with a coverslip.
• Dispose of broken glass properly; know the designated area.

Common Wet Mount Samples

• Elodea Leaf: Use water to suspend.
• Human Cheek Cells: Use methylene blue for staining.
• Onion Cells: Use iodine for staining.
• Pond Water: Various microorganisms can be observed.

Key Learning Objectives

• Master metric conversions; familiarize with the conversions table.
• Define and understand the function of the compound light microscope's main components.
• Calculate total magnification and identify various cell types, their parts, and functions.
• Understand the process and purpose of making a wet mount, along with the necessity of using a medium.

Upcoming Reminders

• Review syllabus and engage with instructor via Canvas for any questions.
• Prepare for the upcoming lab on "Cell Processes."
• Upcoming lab quiz covering microscope topics; bring the specified Scantron form, a pencil, and optionally, a basic calculator.

This quiz covers the essentials of using a compound light microscope, including its components and function. It also addresses laboratory protocols for observing prepared slides. Test your knowledge of microscopy terminology and techniques in this engaging quiz!