Week+2+Microscope+Presentation.pdf

Microscope  Microscope Purpose: to use a compound light microscope to view small cells with more detail than we can see with our naked eyes.  Microscope Pre-Lab Purpose: a self-directed lesson on the use of the Metric System Microscope Pre-Lab ...

Microscope  Microscope Purpose: to use a compound light microscope to view small cells with more detail than we can see with our naked eyes.  Microscope Pre-Lab Purpose: a self-directed lesson on the use of the Metric System Microscope Pre-Lab  Microscope prepared slides: a sample is permanently attached to the slide – Safe for user – Safe for microscope – Lasts for years and years and years… Microscope  Microscope: LENSES Total Magnification:  Microscope: FOV Field of View (FOV): the circle of light that you see (which contains your specimen)  Microscope Notes: – WORK EFFICIENTLY in this long lab: Work with your lab partners. Each partner should prepare and focus a different specimen slide to the best magnification to see details of three whole cells. Then all lab partners can rotate around the table and observe and draw from each microscope. You must make your own drawings and measurements. Copying from someone else’s drawing is considered cheating. Prepared Slide: Letter “e”  Prepared Slide: Spirogyra cell wall: nucleus: chloroplasts:  Prepared Slide: Paramecium cytoplasm: vacuoles: nucleus:  Prepared Slide: Human Blood nucleus: white blood cells (WBCs): red blood cells (RBCs): Prepared Slide: Sickle Cell Anemia Prepared Slide: Frog/Amphibian Blood white blood: red blood:  Microscope: Wet Mount Protocol: 1. Obtain a slide from the container of alcohol. Use a flat slide that contains no depressions. DRY it off with a quarter strip of paper towel. Place it on the lab bench. 2. Place a small drop of water or stain on the slide. 3. Place specimen in the medium. Obtain a coverslip. Where do you dispose of broken glass? ____________ Figure 1. 4. Hold the cover glass at Proper placement an angle of a cover on the slideglass. and allow it to fall cover glass cover glass over the specimen. specimen specimen slide slide  Wet Mount: Elodea Leaf * Use water to suspend sample. chloroplasts:  Wet Mount: Human Cheek * Use methylene blue to suspend sample. nucleus:  Wet Mount: Onion * Use iodine to suspend sample. cell wall: nucleus: Wet Mount: Pond Water  What You Should Know… 1. Be able to make metric conversions (have the conversions table memorized). 2. Define “compound light microscope”. Be prepared to label and describe the function of the main components. 3. How is total magnification calculated? 4. What is a cell? Be familiar with the different types of cells that we looked at today. Be able to identify and define the function of the cell parts we asked you to locate. 5. How is a wet mount made? Why is it used? Why is a medium necessary?  NEXT WEEKS’ REMINDERS: Be sure to review the syllabus and email me at THROUGH CANVAS if you have any questions. Be sure to read the “Cell Processes” lab in advance of class next week. Lab quiz #2: – topics: Microscope – bring a Scantron form 815-E – be sure to bring a pencil (pens are unacceptable for quiz) – basic calculator optional (scratch paper will be provided) must not be able to transmit a signal to the internet