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Questions and Answers
What is the primary purpose of slanting in a microbiology laboratory?
What is the primary purpose of slanting in a microbiology laboratory?
- To reduce the surface area available for bacterial growth
- To prevent contamination of the culture
- To provide a larger surface area for bacterial growth (correct)
- To increase the solubility of nutrients in the medium
Which of the following is NOT a likely outcome of slanting a microbiological culture?
Which of the following is NOT a likely outcome of slanting a microbiological culture?
- Decreased surface area for bacterial growth (correct)
- Reduced risk of contamination
- Increased growth rate of bacteria
- Improved visualization of bacterial colonies
What might be a disadvantage of using slanted cultures in microbiological analysis?
What might be a disadvantage of using slanted cultures in microbiological analysis?
- Decreased surface area for bacterial growth
- Increased risk of contamination (correct)
- Difficulty in observing bacterial morphology
- Improved colony formation
Why is it important to control the angle of the slant in microbiological cultures?
Why is it important to control the angle of the slant in microbiological cultures?
What is a potential limitation of using slanted cultures in microbiological research?
What is a potential limitation of using slanted cultures in microbiological research?
What is the main advantage of using plates in microbiology?
What is the main advantage of using plates in microbiology?
What is a limitation of using liquid media in microbiology?
What is a limitation of using liquid media in microbiology?
Why are plates often preferred over liquid media in microbiology?
Why are plates often preferred over liquid media in microbiology?
What is a key difference between plates and liquid media in microbiology?
What is a key difference between plates and liquid media in microbiology?
What is an advantage of using solid media like plates in microbiology?
What is an advantage of using solid media like plates in microbiology?
What can be inferred about the bacteria Salmonella from the table?
What can be inferred about the bacteria Salmonella from the table?
What is the color of the butt of the medium for Proteus bacteria?
What is the color of the butt of the medium for Proteus bacteria?
What is the significance of the 'A' in the table for Salmonella and Proteus bacteria?
What is the significance of the 'A' in the table for Salmonella and Proteus bacteria?
What can be said about the LF test result for the bacteria in the table?
What can be said about the LF test result for the bacteria in the table?
What is the relationship between the 'Alk' and '+' symbols in the table?
What is the relationship between the 'Alk' and '+' symbols in the table?
What is the primary reason for keeping working areas clear of unnecessary items in a microbiology laboratory?
What is the primary reason for keeping working areas clear of unnecessary items in a microbiology laboratory?
What should a student do if they have a fresh unhealed cut or scratch before beginning a laboratory exercise?
What should a student do if they have a fresh unhealed cut or scratch before beginning a laboratory exercise?
Why are laboratory coats worn in a microbiology laboratory?
Why are laboratory coats worn in a microbiology laboratory?
What is the purpose of pre-sterilizing and capping containers used for specimen collection or culture material?
What is the purpose of pre-sterilizing and capping containers used for specimen collection or culture material?
Why should personal conduct in a microbiology laboratory be quiet and orderly?
Why should personal conduct in a microbiology laboratory be quiet and orderly?
What should a student do if they encounter a problem during a laboratory exercise?
What should a student do if they encounter a problem during a laboratory exercise?
What is the primary characteristic of Colony Morphology when used to identify microorganisms?
What is the primary characteristic of Colony Morphology when used to identify microorganisms?
Which of the following characteristics is NOT typically used to describe Colony Morphology?
Which of the following characteristics is NOT typically used to describe Colony Morphology?
What is the primary purpose of observing the color of a microorganism's colony?
What is the primary purpose of observing the color of a microorganism's colony?
Which of the following characteristics is used to describe the edge of a microorganism's colony?
Which of the following characteristics is used to describe the edge of a microorganism's colony?
What is the purpose of observing the odor of a microorganism's colony?
What is the purpose of observing the odor of a microorganism's colony?
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Study Notes
Safety Procedures and Precautions in Microbiology Laboratory
- Hands and bench tops must be kept clean with disinfectants to prevent contamination
- Laboratory coats should be worn, and long hair should be tied back to maintain a sterile environment
- Working areas should be kept clear of unnecessary items to avoid distractions and potential hazards
- Containers used for specimen collection or culture material should be pre-sterilized and capped to prevent unsterile air from entering
- Nothing should be placed in the mouth, and personal conduct in the laboratory should be quiet and orderly
- Instructors should be consulted promptly whenever problems arise
- Students with fresh, unhealed cuts, scratches, burns, or other injuries should notify the instructor before beginning or continuing with the experiment
Importance of Slant and Plate Culture
- Slant culture provides a greater surface area for bacterial growth in a tube
- Plate culture is particularly helpful in isolating a specific species of bacteria, which is not possible in a liquid medium
- Plate culture can aid in identifying bacteria
Colony Morphology on Solid Medium
- Characteristics of bacterial colonies on solid medium include:
- Shape
- Elevation
- Size
- Margin
- Surface
- Density
- Color
- Presence of pigment
- Odor
Examples of Bacterial Characteristics
- Salmonella:
- Ferments glucose with H2S production
- Appears black in the butt of the tube
- Produces a red color
- Produces a yellow pigment
- Proteus:
- Ferments glucose without H2S production
- Appears alkaline (A/Alk/-)
- Produces no pigment (LF)
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