26 Questions
What is the primary purpose of slanting in a microbiology laboratory?
To provide a larger surface area for bacterial growth
Which of the following is NOT a likely outcome of slanting a microbiological culture?
Decreased surface area for bacterial growth
What might be a disadvantage of using slanted cultures in microbiological analysis?
Increased risk of contamination
Why is it important to control the angle of the slant in microbiological cultures?
To ensure even distribution of nutrients
What is a potential limitation of using slanted cultures in microbiological research?
Difficulty in standardizing growth conditions
What is the main advantage of using plates in microbiology?
To isolate a specific species of bacteria
What is a limitation of using liquid media in microbiology?
They allow for the isolation of specific bacterial species
Why are plates often preferred over liquid media in microbiology?
They allow for the isolation of specific bacterial species
What is a key difference between plates and liquid media in microbiology?
Plates allow for the isolation of specific bacterial species
What is an advantage of using solid media like plates in microbiology?
They allow for the growth of microorganisms in a controlled environment
What can be inferred about the bacteria Salmonella from the table?
It does not produce H2S.
What is the color of the butt of the medium for Proteus bacteria?
Black
What is the significance of the 'A' in the table for Salmonella and Proteus bacteria?
It indicates the fermentation of glucose.
What can be said about the LF test result for the bacteria in the table?
It is not shown in the table.
What is the relationship between the 'Alk' and '+' symbols in the table?
The '+' symbol indicates the production of alkaline substances.
What is the primary reason for keeping working areas clear of unnecessary items in a microbiology laboratory?
To prevent contamination of the work area
What should a student do if they have a fresh unhealed cut or scratch before beginning a laboratory exercise?
Notify the instructor before beginning the exercise
Why are laboratory coats worn in a microbiology laboratory?
To prevent the spread of microorganisms from the wearer's clothing
What is the purpose of pre-sterilizing and capping containers used for specimen collection or culture material?
To prevent contamination of the container
Why should personal conduct in a microbiology laboratory be quiet and orderly?
To prevent accidents caused by sudden movements
What should a student do if they encounter a problem during a laboratory exercise?
Consult with the instructor promptly
What is the primary characteristic of Colony Morphology when used to identify microorganisms?
It is used to observe the microorganism's growth on solid medium
Which of the following characteristics is NOT typically used to describe Colony Morphology?
Motility
What is the primary purpose of observing the color of a microorganism's colony?
To determine the microorganism's ability to produce pigments
Which of the following characteristics is used to describe the edge of a microorganism's colony?
Margin
What is the purpose of observing the odor of a microorganism's colony?
To determine the microorganism's metabolic byproducts
Study Notes
Safety Procedures and Precautions in Microbiology Laboratory
- Hands and bench tops must be kept clean with disinfectants to prevent contamination
- Laboratory coats should be worn, and long hair should be tied back to maintain a sterile environment
- Working areas should be kept clear of unnecessary items to avoid distractions and potential hazards
- Containers used for specimen collection or culture material should be pre-sterilized and capped to prevent unsterile air from entering
- Nothing should be placed in the mouth, and personal conduct in the laboratory should be quiet and orderly
- Instructors should be consulted promptly whenever problems arise
- Students with fresh, unhealed cuts, scratches, burns, or other injuries should notify the instructor before beginning or continuing with the experiment
Importance of Slant and Plate Culture
- Slant culture provides a greater surface area for bacterial growth in a tube
- Plate culture is particularly helpful in isolating a specific species of bacteria, which is not possible in a liquid medium
- Plate culture can aid in identifying bacteria
Colony Morphology on Solid Medium
- Characteristics of bacterial colonies on solid medium include:
- Shape
- Elevation
- Size
- Margin
- Surface
- Density
- Color
- Presence of pigment
- Odor
Examples of Bacterial Characteristics
- Salmonella:
- Ferments glucose with H2S production
- Appears black in the butt of the tube
- Produces a red color
- Produces a yellow pigment
- Proteus:
- Ferments glucose without H2S production
- Appears alkaline (A/Alk/-)
- Produces no pigment (LF)
This quiz covers the essential safety procedures and precautions to be taken when working with microorganisms like bacteria and fungi in a laboratory setting.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
