Podcast
Questions and Answers
What is the normal adult cut-off point for triglycerides?
What is the normal adult cut-off point for triglycerides?
Which dyslipidemia is a result of imbalance between synthesis and clearance of VLDL in the circulation?
Which dyslipidemia is a result of imbalance between synthesis and clearance of VLDL in the circulation?
Which gene is defective in Tangier’s disease?
Which gene is defective in Tangier’s disease?
Which fasting hour is preferred for total cholesterol testing?
Which fasting hour is preferred for total cholesterol testing?
Signup and view all the answers
Which is the reference method for total cholesterol measurement?
Which is the reference method for total cholesterol measurement?
Signup and view all the answers
Which characteristic implicates combined hyperlipidemia?
Which characteristic implicates combined hyperlipidemia?
Signup and view all the answers
Which principle is involved in cholesterol measurement using the Abell-Kendall method?
Which principle is involved in cholesterol measurement using the Abell-Kendall method?
Signup and view all the answers
What is the desirable adult cholesterol level?
What is the desirable adult cholesterol level?
Signup and view all the answers
According to the NCEP ATP III guidelines, how would you classify a triglyceride level of 300 mg/dL?
According to the NCEP ATP III guidelines, how would you classify a triglyceride level of 300 mg/dL?
Signup and view all the answers
What is the sensitivity of the cholesterol method found in the module?
What is the sensitivity of the cholesterol method found in the module?
Signup and view all the answers
Which type of fatty acid lowers the risk of cardiovascular disease?
Which type of fatty acid lowers the risk of cardiovascular disease?
Signup and view all the answers
Which enzyme is the rate-limiting step in the biosynthesis of cholesterol?
Which enzyme is the rate-limiting step in the biosynthesis of cholesterol?
Signup and view all the answers
What is the major protein of HDL?
What is the major protein of HDL?
Signup and view all the answers
Which lipoprotein is exclusively found in chylomicrons?
Which lipoprotein is exclusively found in chylomicrons?
Signup and view all the answers
Which lipoprotein is the major carrier of endogenous triglycerides?
Which lipoprotein is the major carrier of endogenous triglycerides?
Signup and view all the answers
Which specimen is most often used to determine total proteins?
Which specimen is most often used to determine total proteins?
Signup and view all the answers
Which of the following can increase the test result of total protein determination?
Which of the following can increase the test result of total protein determination?
Signup and view all the answers
What is true of HDL particles?
What is true of HDL particles?
Signup and view all the answers
Study Notes
Lipid Metabolism
- Fatty acid with 12 carbon atoms is classified as medium-chain.
- Polyunsaturated fatty acids lower the risk of cardiovascular disease.
Cholesterol Biosynthesis
- HMG-CoA reductase is the rate-limiting enzyme in cholesterol biosynthesis.
Lipoproteins
- Apo A-I is the major protein of HDL.
- Apo B-48 is exclusively found in chylomicrons.
- VLDL is the major carrier of endogenous triglycerides.
Lipid Profile
- Total protein levels decrease in recumbent position.
- At birth, total protein concentration is lower, and adult levels are reached by age 3.
- Lipemia, sample hemolysis, and dehydration can increase total protein determination results.
HDL Particles
- Spherical HDL particles contain 3 apoA-1 molecules.
- Discoidal HDL is less common and more active.
Lipoprotein Function
- Chylomicrons principally carry exogenous cholesterol.
- VLDL carries endogenous triglycerides.
Dyslipidemia
- Combined hyperlipoproteinemia results from an imbalance between VLDL synthesis and clearance.
- Tangier's disease is caused by a defective ABCA1 gene.
Lipid Testing
- 12-hour fasting is preferred for total cholesterol testing.
- The reference method for total cholesterol measurement is Isotope Dilution Mass Spectrometry.
Triglyceride Measurement
- The Van Handel & Zilversmith method determines triglycerides.
- Designated calibrated blanking is used to correct for endogenous glycerol in triglyceride measurement.
Hyperlipidemia
- Combined hyperlipidemia is characterized by increased triglycerides and cholesterol.
- Abell-Kendall method is used for cholesterol measurement, which involves an enzymatic, 2-step process.
Lipid Determination
- Serum is the preferred sample for lipid determination.
- A triglyceride level of 300 mg/dL is classified as very high according to NCEP ATP III guidelines.
- A cholesterol level of 300 mg/dL is classified as high risk.
Lipoprotein Classification
- Chylomicrons are the lowest in density and the largest lipoprotein.
- VLDL is formed in the liver and carries endogenous triglycerides.
- LDL is the major cholesterol carrier.
- HDL is also known as "good cholesterol".
- IDL is referred to as the transitional form.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
Description
This quiz tests your knowledge of fatty acids, their classification, and their role in cholesterol biosynthesis. It covers topics such as the characteristics of different fatty acid chains, the benefits of specific fatty acids, and the enzymes involved in cholesterol production.
