Biology Class: Squamous Epithelium Slide Preparation

Give the functions of squamous epithelium.

Cells of squamous epithelium rest on __________.

Squamous epithelium performs the function of __________.

Epithelium of bronchi is __________.

Intercellular material is negligible in __________ tissue.

Ciliated epithelium is found in lining of __________.

What is alternative name for squamous epithelium?

Aim and Requirements

Aim: To prepare a temporary stained slide of squamous epithelium
Requirements: Live material of concerned tissue, methylene blue, glass slides, cover slips, dropper, glycerin, NaCl solution (0.9% w/v), toothpick, water, compound microscope

Procedure

Rinse mouth well with water and gently scrap the inside of your cheek with the broad end of a clean toothpick and discard this material.
Add a drop of 0.9% NaCl solution and a drop of methylene blue with the help of a dropper.
After two minutes, remove the excess stain and saline using the edge of a filter paper.
Now, put a drop of glycerin on the cells. Place a coverslip over the tissue and gently press it with the back of a pencil to spread the cells.
Examine the slide under the low power of microscope. Draw a labelled diagram of your preparation.

Observation

Observe the slide under low power and then under high power
Cells are thin, polygonal, nucleated without intracellular spaces, cells are held together by cementing material

Questions

In which tissue the cells are flat and polygonal?
What is alternative name for squamous epithelium?

This quiz covers the procedures for preparing a temporary stained slide of squamous epithelium. Students will learn about the materials required and the steps involved, including staining techniques and microscopy. A focus on observation skills is essential as you analyze the cellular structure under different magnifications.