Biology Chapter: Water Properties & Microscopy
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Questions and Answers

What is indicated by a high electronegativity delta in a covalent bond?

  • The bond is ionic.
  • The bond is covalent but weak.
  • The bond is nonpolar.
  • The bond is polar. (correct)

Which property of water allows it to hold onto other charged surfaces?

  • Viscosity
  • Adhesion (correct)
  • Cohesion
  • Buoyancy

What is a characteristic of water's high specific heat?

  • It loses heat rapidly.
  • It requires a lot of energy to change temperature. (correct)
  • It can heat up quickly.
  • It has low thermal conductivity.

In the context of water, what does buoyancy refer to?

<p>The upward force exerted by water on an object. (A)</p> Signup and view all the answers

How does viscosity relate to water compared to honey?

<p>Honey is more viscous than water. (A)</p> Signup and view all the answers

What is the main function of the light microscope?

<p>To magnify living samples using light and lenses (D)</p> Signup and view all the answers

Which microscopy technique uses electric beams to enhance resolution?

<p>Electron microscopy (A)</p> Signup and view all the answers

What is a primary feature of cryogenic electron microscopy?

<p>It freezes samples and uses gentler electron beams (B)</p> Signup and view all the answers

What are the end products of glycolysis?

<p>2 Pyruvate and NADH (D)</p> Signup and view all the answers

During which phase of cellular respiration is 34 ATP generated?

<p>Electron transport chain (C)</p> Signup and view all the answers

Which microscopy technique is specifically useful for viewing proteins?

<p>Cryogenic electron microscopy (B)</p> Signup and view all the answers

What is the role of centrifugation in cell fractionation?

<p>To separate cellular components based on their density (A)</p> Signup and view all the answers

What is the primary outcome of the Krebs cycle?

<p>Production of ATP and carbon dioxide (C), Formation of NADH and FADH2 (D)</p> Signup and view all the answers

Which method directly stains specific cell parts using antibodies?

<p>Direct immunofluorescence (D)</p> Signup and view all the answers

What happens to samples in electron microscopy?

<p>They are killed due to the method used (A)</p> Signup and view all the answers

What is the main difference between dehydration synthesis and hydrolysis?

<p>Dehydration synthesis creates bonds while hydrolysis breaks them. (D)</p> Signup and view all the answers

Which statement correctly describes the levels of protein structure?

<p>Primary structure is a linear sequence of amino acids. (C)</p> Signup and view all the answers

How does competitive inhibition differ from noncompetitive inhibition?

<p>In competitive inhibition, an inhibitor binds to the active site. (B)</p> Signup and view all the answers

Which characteristic is true for eukaryotes but not for prokaryotes?

<p>Eukaryotes are structurally more complex. (D)</p> Signup and view all the answers

Which of the following organelles is found in plant cells but not in animal cells?

<p>Chloroplasts (B)</p> Signup and view all the answers

Which of the following correctly compares primary and secondary protein structures?

<p>Secondary structure forms a coil from amino acid chains. (B)</p> Signup and view all the answers

What is a typical feature of prokaryotes?

<p>They lack membrane-bound organelles. (B)</p> Signup and view all the answers

Which feature distinguishes the function of competitive inhibition from noncompetitive inhibition?

<p>Competitive inhibitors can be overcome by substrate concentration. (B)</p> Signup and view all the answers

What is the primary requirement for active transport across a membrane?

<p>It requires energy to move substances against their concentration gradient. (C)</p> Signup and view all the answers

Which transport process involves the movement of water from areas of low solute concentration to areas of high solute concentration?

<p>Osmosis (B)</p> Signup and view all the answers

What happens to an animal cell placed in a hypotonic solution?

<p>It bursts due to water influx. (B)</p> Signup and view all the answers

Which process is characterized by the creation of a vacuole for moving particles into the cell?

<p>Endocytosis (A)</p> Signup and view all the answers

Which function of membrane proteins is primarily related to the communication between cells?

<p>Signal transduction (A)</p> Signup and view all the answers

What is the purpose of facilitated diffusion?

<p>To allow the passage of substances across a membrane with protein assistance. (D)</p> Signup and view all the answers

If an animal cell is placed in distilled water, what will happen?

<p>The cell will remain largely unchanged like in an isotonic solution. (A)</p> Signup and view all the answers

What is the definition of phagocytosis?

<p>The absorption of pathogens into the cell for destruction. (A)</p> Signup and view all the answers

What is the primary function of chloroplasts in a cell?

<p>Create glucose for the cell. (D)</p> Signup and view all the answers

Which organelle is mainly responsible for generating ATP?

<p>Mitochondria (D)</p> Signup and view all the answers

What is the main role of the cell membrane?

<p>Regulate entry and exit of materials. (A)</p> Signup and view all the answers

Which organelle is involved in the modification and shipping of proteins?

<p>Golgi Apparatus (A)</p> Signup and view all the answers

What function does the vacuole serve in a cell?

<p>Store nutrients and other matter. (A)</p> Signup and view all the answers

What is the main purpose of the lysosome?

<p>Clean the cell and eliminate waste. (B)</p> Signup and view all the answers

Which organelle is responsible for the production of ribosomes?

<p>Nucleus (B)</p> Signup and view all the answers

What is the function of the rough endoplasmic reticulum?

<p>Modify proteins through protein synthesis. (D)</p> Signup and view all the answers

What is the primary function of the cell wall?

<p>Provide structural support and protection. (A)</p> Signup and view all the answers

What is the primary function of the nuclear membrane?

<p>Protect the nucleus. (B)</p> Signup and view all the answers

Which functional group is represented by the structure shown in the first image?

<p>Phosphate (C)</p> Signup and view all the answers

What role does the end product play in the process of end-product inhibition?

<p>It acts as a noncompetitive inhibitor. (D)</p> Signup and view all the answers

What is the calculated size of one graticule unit at 40x magnification?

<p>2.35 micrometers (A)</p> Signup and view all the answers

Which of the following statements about functional groups is true?

<p>Amino groups contain a nitrogen atom bonded to two hydrogen atoms. (A)</p> Signup and view all the answers

What type of inhibition is characterized by the end product of a metabolic pathway preventing further production?

<p>Noncompetitive inhibition (C)</p> Signup and view all the answers

Which functional group is likely to be involved in forming hydrogen bonds?

<p>All of the above (D)</p> Signup and view all the answers

Which functional group is known for its role in energy transfer within cells?

<p>Phosphate group (D)</p> Signup and view all the answers

How is end-product inhibition critical for metabolic pathways?

<p>It helps maintain balance by preventing overproduction of metabolites. (D)</p> Signup and view all the answers

Flashcards

Cohesion

The tendency of water molecules to be attracted to each other due to their hydrogen bonds.

Adhesion

The tendency of water molecules to be attracted to other surfaces due to their polarity.

Thermal Conductivity

The ability of water to transfer heat quickly from one location to another.

Specific Heat

The amount of energy required to raise the temperature of water by one degree.

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Buoyancy

The upward force exerted on an object in a fluid.

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What is the main function of mitochondria?

Mitochondria are organelles responsible for generating adenosine triphosphate (ATP), the primary energy source for cellular processes.

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What is the main function of chloroplasts?

Chloroplasts are organelles found in plant cells responsible for photosynthesis, the process of converting light energy into chemical energy in the form of glucose.

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What is the primary function of the cell membrane?

The cell membrane is a selectively permeable barrier that regulates the passage of substances into and out of the cell, ensuring a controlled environment for cellular processes.

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What is the primary function of the cell wall?

The cell wall provides structural support and protection for plant cells, helping to maintain their shape and rigidity.

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What is the primary function of the nuclear membrane?

The nuclear membrane, also known as the nuclear envelope, encloses the genetic material (DNA) within the nucleus, protecting it from the cytoplasm and ensuring its proper function.

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What is the primary function of the nucleus?

The nucleus is the control center of the cell, containing the genetic material (DNA and RNA) responsible for regulating cellular processes and passing on genetic information.

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What is the primary function of the nucleolus?

The nucleolus is a structure within the nucleus responsible for producing ribosomes, essential for protein synthesis.

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What is the main function of the Golgi apparatus?

The Golgi apparatus acts as a packaging and distribution center for proteins synthesized by the cell. It modifies, sorts, and packages proteins into vesicles for transport within or outside the cell.

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What is the primary function of ribosomes?

Ribosomes are the protein synthesis machinery of the cell, responsible for translating genetic information from mRNA into proteins.

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What is the primary function of the smooth endoplasmic reticulum?

The smooth endoplasmic reticulum (SER) functions in synthesizing lipids and phospholipids, components of cell membranes, and detoxifying harmful substances.

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Passive Transport

Movement of molecules across a membrane from an area of high concentration to an area of low concentration. It does not require energy.

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Active Transport

Movement of molecules across a membrane against their concentration gradient. It requires energy.

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Osmosis

The movement of water molecules across a selectively permeable membrane from an area of high water concentration to an area of low water concentration.

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Facilitated Diffusion

The movement of molecules across a membrane with the help of a protein. It does not require energy directly, but relies on the concentration gradient.

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Exocytosis

The process by which a cell releases substances into the extracellular space by enclosing them in a membrane-bound vesicle that fuses with the plasma membrane.

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Endocytosis

The process by which a cell takes in materials from the extracellular space by engulfing them in a membrane-bound vesicle.

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Phagocytosis

A type of endocytosis where the cell engulfs large particles, such as bacteria or cellular debris, into a phagosome.

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Membrane Proteins: Transport

Membrane proteins facilitate the movement of substances across the cell membrane. They help move molecules in and out of the cell.

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Light Microscopy

A type of microscopy that uses light to illuminate and magnify a sample, typically for viewing living specimens.

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Electron Microscopy

A powerful microscopy technique that uses electron beams to create highly magnified images of samples, providing detailed views of cells and their internal structures.

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Cryogenic Electron Microscopy

A type of electron microscopy that freezes samples to preserve their structures and uses gentler electron beams to create images. Useful for studying proteins and other delicate structures.

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Immunofluorescence

A technique that uses fluorescent dyes or antibodies to label specific molecules or structures within a cell, enabling visualization of their location and distribution.

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Freeze Fracture

A technique that exposes frozen cell samples to a fracturing force, exposing their inner membranes and proteins. Useful for examining 3D structures of cells.

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Cell Fractionation

A process for isolating and separating different cellular components, such as organelles, by disrupting cells and using centrifugal force

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Centrifugation

A technique that uses centrifugal force to separate different components of a mixture based on their density and size, often used after cell fractionation.

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Glycolysis

The first stage in cellular respiration, occurring in the cytoplasm, where glucose is broken down into pyruvate, producing a small amount of ATP and NADH.

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Krebs Cycle

A series of chemical reactions that occur in the mitochondrial matrix, generating ATP, NADH, and FADH2 from pyruvate.

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Electron Transport Chain (ETC)

The final stage of cellular respiration, where electrons from NADH and FADH2 are passed along a series of protein complexes in the mitochondrial inner membrane, generating a large amount of ATP.

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Dehydration synthesis vs. Hydrolysis

Dehydration synthesis removes water to form a bond, while hydrolysis adds water to break a bond.

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Levels of protein structure

Primary: Chain of amino acids. Secondary: Coiled or folded due to hydrogen bonds. Tertiary: 3D shape due to interactions between R groups. Quaternary: Multiple polypeptide chains interacting.

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Competitive vs. Noncompetitive Inhibition

Competitive inhibition: A substance blocks the active site on an enzyme, preventing substrate binding. Noncompetitive inhibition: A substance binds to an allosteric site, changing the shape of the active site and preventing substrate binding.

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Prokaryotes vs. Eukaryotes

Prokaryotes are simple cells without a nucleus. Eukaryotes are complex cells with a nucleus, membrane-bound organelles, and a more organized structure.

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Plant cells vs. Animal cells

Plant cells have a large vacuole, chloroplasts, and a cell wall. Animal cells lack these organelles.

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What is end-product inhibition?

The inhibition of an enzyme by the end product of the reaction sequence it catalyzes. This feedback mechanism helps regulate metabolic pathways and prevent the overproduction of unnecessary products.

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How does end-product inhibition work?

The end product acts as a noncompetitive inhibitor, binding to a site distinct from the active site of the enzyme. This binding alters the enzyme's conformation, reducing its activity and slowing down the production of the end product.

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What is a stage micrometer?

A stage micrometer is a tool used to calibrate a microscope's field of view. It features a precise scale, often in micrometers (µm), allowing for accurate measurement of objects under the microscope.

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How do you calculate the size of a graticule unit at 40x magnification?

To determine the size of one graticule unit, you need two measurements: the stage micrometer scale reading and the number of graticule units that align with it. The stage micrometer scale reading (in µm) is divided by the number of graticule units to calculate the size of each unit. This calculation provides the measurement of one graticule unit at the specific magnification used.

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What is the phosphate functional group?

The phosphate functional group consists of a phosphorus atom bonded to four oxygen atoms, commonly found in molecules like ATP and DNA, where it plays a crucial role in energy transfer and genetic information.

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What is the amino functional group?

The amino functional group comprises a nitrogen atom bonded to two hydrogen atoms (NH2), found in amino acids, which are the building blocks of proteins. It plays a crucial role in protein structure and function.

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What is the carboxyl functional group?

The carboxyl functional group contains a carbon atom double-bonded to an oxygen atom and also single-bonded to a hydroxyl group (COOH). It's found in amino acids and fatty acids, contributing to their acidic properties.

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What is the hydroxyl functional group?

The hydroxyl functional group consists of an oxygen atom bonded to a hydrogen atom (OH). It's found in alcohols and sugars, contributing to the polarity and solubility of organic molecules.

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Study Notes

Independent, Dependent, and Controlled Variables

  • Independent Variable (IDV): The variable changed to observe the outcome.
  • Dependent Variable (DV): The variable affected by the IDV, the result.
  • Controlled Variable: Variables kept constant to isolate the effect of the IDV.

Bond Types and Formation

  • Nonpolar Covalent Bond: Electrons shared equally between atoms with similar electronegativity. Forms between similar atoms.
  • Polar Covalent Bond: Electrons shared unequally between atoms with different electronegativity. Forms between different atoms.
  • Ionic Bond: One atom gives up electrons to another to achieve full valence shells. Forms between highly electronegative and electropositive atoms.
  • Hydrogen Bond: A weak bond between a hydrogen atom (covalently bonded to an electronegative atom) and an electronegative atom in another molecule.

Electronegativity

  • Electronegativity: An atom's ability to attract and hold electrons.

Relationship between Electronegativity and Covalent Bonds

  • High electronegativity difference results in polar covalent bonds.
  • Low electronegativity difference results in nonpolar covalent bonds.

Properties of Water

  • Cohesion: Water molecules attract each other; e.g., surface tension.
  • Adhesion: Water molecules attract other charged substances; e.g., water sticking to plant cell walls.
  • Thermal Conductivity: Water transfers heat efficiently.
  • High Specific Heat: Water requires much energy to change temperature.
  • Buoyancy: Upward force on an object in water.
  • Viscosity: The thickness of a liquid.

Biological Molecules

  • Carbohydrates:
    • Elements: C, H, O
    • Monosaccharides
    • Glycosidic linkages
    • Energy storage, structural support
    • Examples: Glucose, Fructose
  • Proteins:
    • Elements: C, H, N, O, P
    • Amino acids
    • Peptide bonds
    • Structural support, enzymes, transport, hormones
  • Nucleic Acids:
    • Elements: C, H, N, O, P
    • Nucleotides
    • Phosphodiester linkages
    • Store and transmit genetic information
    • Examples: DNA, RNA
  • Lipids:
    • Elements: C, H, O
    • Glycerol and fatty acids
    • Ester linkages
    • Insulation, energy storage, structural components of cell membranes
    • Examples: Fats, Steroids, Phospholipids

Fatty Acids

  • Saturated: No double bonds between carbon atoms.
  • Monounsaturated: One double bond between carbon atoms.
  • Polyunsaturated: Two or more double bonds between carbon atoms.
  • Cis: Hydrogens on the same side of the double bond.
  • Trans: Hydrogens on opposite sides of the double bond.

Enzyme Function

  • Induced Fit Model: Enzymes change shape to fit the substrate.
  • Substrate concentration and product formation: Increased substrate concentration increases the rate until a max rate is reached. Increased substrate concentration above the max rate has no effect on reaction rate
  • Temperature and Enzyme Activity: Optimal temperature results in an increased rate, however, very high temperatures denature the enzyme.
    • pH and Enzyme Activity: Optimal pH results in an increased rate, however, very high or very low pH values denature enzymes.

Cell Structures

  • Centrioles: Involved in cell division.
  • Nucleus: Contains DNA.
  • Nucleolus: Produces ribosomes.
  • Nuclear envelope: Membrane surrounding the nucleus.
  • Lysosome: Contains enzymes to digest waste.
  • Smooth ER: Lipid synthesis.
  • Rough ER: Protein synthesis.
  • Golgi apparatus: Processes and packages proteins.
  • Ribosomes: Protein synthesis.
  • Cytosol: Cytoplasm outside the organelles.
  • Cell membrane: Encloses the cell.
  • Mitochondria: ATP production (energy)
  • Chloroplasts: Photosynthesis (energy production in plant cells).
  • Vacuoles: Storage.

Microscopy Techniques

  • Light Microscope: Visualizes living samples.
  • Electron Microscope: Higher magnification viewing dead cells.

Cellular Respiration

  • Glycolysis: Splits glucose to produce pyruvate. Occurs in the cytoplasm.
  • Krebs Cycle (Citric Acid Cycle): Produces ATP, NADH, and FADH2. Occurs in the matrix of the mitochondria.
  • Electron Transport Chain (ETC): Produces the most ATP (34). Occurs in the inner mitochondrial membrane.
  • Cellular Respiration Equation: C6H12O6 + 6O2 → 6CO2 + 6H2O + ATP

Photosynthesis

  • Light-Dependent Reactions: Capture light energy to make NADPH and ATP. Occurs in the thylakoid membrane of chloroplasts.
  • Calvin Cycle (Light-Independent Reactions): Uses NADPH and ATP to build glucose. Occurs in the stroma of chloroplasts.

Food Chains and Food Webs

  • Food Chain: A linear sequence of energy transfer from one organism to the next.
  • Food Web: A network of interconnected food chains.
  • Energy Pyramid: A graphical representation of energy flow through trophic levels; the pyramid is wider at the base (producers), with progressively narrower tiers for higher trophic levels.

Transport Processes

  • Active Transport: Movement against concentration gradient using energy.
  • Passive Transport: Movement with concentration gradient without energy.
  • Diffusion: Movement from high to low concentration.
  • Osmosis: Movement of water from low to high solute concentration.

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Bio Final Review Guide

Description

Test your knowledge on water properties, cellular respiration, and microscopy techniques. This quiz covers topics like electronegativity in covalent bonds, specific heat of water, and the functions of various microscopy methods. Perfect for biology students wanting to assess their understanding of this chapter.

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