Biology Chapter - Molecular Structures and Prokaryotes
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Questions and Answers

Which of the following biological structures is closest in size to a typical globular protein?

  • Rhinovirus
  • Insulin (correct)
  • Microfilaments/Hb
  • Mitochondria/Bacteria
  • Which of the following statements accurately describes the relationship between size and resonance frequency in biological objects?

  • Smaller objects have lower resonance frequencies.
  • Resonance frequency is directly proportional to object volume.
  • Resonance frequency is independent of object size.
  • Smaller objects have higher resonance frequencies. (correct)
  • Which of these biological components would be most likely to exhibit significant quantum effects?

  • A mitochondrion
  • A eukaryotic cell
  • A single amino acid (correct)
  • A strand of DNA
  • Which of the following is NOT a dominant force or effect at the nanoscale?

    <p>Gravity (A)</p> Signup and view all the answers

    Which of the following biological structures is most similar in size to a lysosome or peroxisome?

    <p>Small bacteria (A)</p> Signup and view all the answers

    What type of environment did the early Earth's atmosphere provide?

    <p>Reducing (D)</p> Signup and view all the answers

    What was a key finding of Miller's experiments in the 1950s?

    <p>Spontaneous synthesis of organic molecules, including amino acids (A)</p> Signup and view all the answers

    What molecule is considered to have been the primary genetic material in the RNA world?

    <p>RNA (A)</p> Signup and view all the answers

    What is the defining characteristic of prokaryotic cells?

    <p>Absence of a nucleus (B)</p> Signup and view all the answers

    Which of the following is NOT a characteristic of prokaryotic cells?

    <p>Contain complex internal membrane systems (B)</p> Signup and view all the answers

    What are the two major groups of prokaryotes?

    <p>Eubacteria and archaebacteria (C)</p> Signup and view all the answers

    What is a key difference between archaebacteria and eubacteria?

    <p>Archaebacteria are found in more extreme environments than eubacteria (C)</p> Signup and view all the answers

    What is a characteristic of thermoacidophiles?

    <p>They live in hot, acidic environments (A)</p> Signup and view all the answers

    What is the range of DNA base pairs found in most bacterial cells?

    <p>0.6 to 5 million (B)</p> Signup and view all the answers

    What is the estimated time period for prokaryotic evolution?

    <p>1 to 1.5 billion years (B)</p> Signup and view all the answers

    What is the primary function of the endoplasmic reticulum?

    <p>Production of proteins and lipids (A)</p> Signup and view all the answers

    What is the primary function of the Golgi apparatus?

    <p>Processing and sorting of proteins and lipids (D)</p> Signup and view all the answers

    Which of the following is TRUE about the endosymbiotic theory?

    <p>It states that mitochondria and chloroplasts were once free-living bacteria that were engulfed by eukaryotic cells. (B)</p> Signup and view all the answers

    What is the role of the outer membrane of mitochondria?

    <p>Regulation of the passage of molecules into and out of the mitochondrion (B)</p> Signup and view all the answers

    What is the location of enzymes involved in oxidative phosphorylation?

    <p>Inner membrane of mitochondria (A)</p> Signup and view all the answers

    What is the main difference between transport vesicles and secretory vesicles?

    <p>Transport vesicles are involved in intracellular transport, while secretory vesicles are involved in transporting molecules outside the cell. (B)</p> Signup and view all the answers

    What is the significance of the presence of DNA, RNA, and ribosomes in mitochondria and chloroplasts?

    <p>All of the above. (D)</p> Signup and view all the answers

    What is the main difference between the smooth ER and the rough ER?

    <p>The rough ER is studded with ribosomes, while the smooth ER is not. (C)</p> Signup and view all the answers

    What is a key difference between prokaryotic cells and eukaryotic cells?

    <p>Eukaryotic cells have a nucleus, while prokaryotic cells do not. (C)</p> Signup and view all the answers

    Which of the following statements best describes the cell wall of E. coli?

    <p>It is a rigid layer composed of polysaccharides and peptides, providing structural support. (B)</p> Signup and view all the answers

    Which of the following features is NOT a typical characteristic of eukaryotic cells?

    <p>Presence of a cell wall (C)</p> Signup and view all the answers

    What is a primary reason why the evolution of eukaryotic cells from prokaryotes is considered a significant challenge in evolutionary biology?

    <p>The large and complex size of eukaryotic cells, requiring multiple evolutionary steps. (B)</p> Signup and view all the answers

    Which of the following statements best describes the organization of DNA in E. coli?

    <p>It is a single circular molecule located in the cytoplasm. (A)</p> Signup and view all the answers

    What is a key difference between the DNA of eukaryotic cells and the DNA of prokaryotic cells?

    <p>Eukaryotic DNA is organized into multiple linear chromosomes, while prokaryotic DNA is a single circular molecule. (C)</p> Signup and view all the answers

    Which of the following statements is FALSE about unicellular eukaryotes?

    <p>They are simpler than bacterial cells. (B)</p> Signup and view all the answers

    Which of the following best describes the term 'compartmentalization' as it applies to eukaryotic cells?

    <p>The organization of specific functions within membrane-bound organelles. (D)</p> Signup and view all the answers

    Which of these statements describes the primary function of mitochondria? (Select one)

    <p>Mitochondria are involved in ATP production through oxidative metabolism. (B)</p> Signup and view all the answers

    What is the primary evidence supporting the endosymbiotic theory of the origin of mitochondria and chloroplasts? (Select one)

    <p>Both organelles contain their own DNA, which is distinct from the nuclear genome of the cell. (B)</p> Signup and view all the answers

    Which of the following is NOT a key feature of the viral eukaryogenesis hypothesis? (Select one)

    <p>The nucleus evolved from a large DNA virus that engulfed a bacteria. (C)</p> Signup and view all the answers

    According to the viral eukaryogenesis hypothesis, which of the following functions remained relatively unchanged after the formation of the nucleus? (Select one)

    <p>ATP production. (B)</p> Signup and view all the answers

    What is the evolutionary significance of the development of ATP synthesis? (Select one)

    <p>It provided cells with a more efficient way to utilize energy and allowed for the development of more complex metabolic pathways. (D)</p> Signup and view all the answers

    Which of the following is NOT a key stage in the evolution of ATP synthesis? (Select one)

    <p>Cellular respiration. (D)</p> Signup and view all the answers

    Which of the following statements accurately compares mitochondria and chloroplasts? (Select one)

    <p>Both mitochondria and chloroplasts are believed to have originated from bacteria through endosymbiosis. (C)</p> Signup and view all the answers

    What is the significance of the statement that mitochondria and chloroplasts have their own DNA? (Select one)

    <p>All of the above. (D)</p> Signup and view all the answers

    According to the viral eukaryogenesis hypothesis, why did the virus evolve into the eukaryotic nucleus? (Select one)

    <p>The virus was able to control the host cell's genetic information more effectively by integrating with its nucleus. (B)</p> Signup and view all the answers

    Which of the following statements describes the role of the cell in the viral eukaryogenesis hypothesis? (Select one)

    <p>The cell provided the virus with a mechanism for gene translation. (C)</p> Signup and view all the answers

    Which of the following scientists is NOT credited with contributing to the development of cellular theory?

    <p>Louis Pasteur (C)</p> Signup and view all the answers

    Which of the following statements accurately describes the relationship between physiology, cellular physiology, and molecular biology?

    <p>Cellular physiology and molecular biology are distinct fields, while physiology integrates both. (C)</p> Signup and view all the answers

    Which of the following elements accounts for the highest percentage of a cell's net weight?

    <p>Oxygen (B)</p> Signup and view all the answers

    Which of the following concepts is NOT directly linked to the development of modern medicine?

    <p>Evolution of the cellular concept (C)</p> Signup and view all the answers

    What is the approximate empirical formula for a cell, based on the four most abundant elements?

    <p>C4H7O2N1 (B)</p> Signup and view all the answers

    Flashcards

    Modern Physiology

    A synthetic academic discipline encompassing multiple areas like classical physiology and molecular biology.

    Cell Theory

    All living organisms are composed of cells, established by Schleiden and Schwann in 1838.

    Basic Cell Composition

    Cells consist mainly of six elements: C, O, H, N, Ca, and P, which account for 99% of their weight.

    Cellular Pathology

    Virchow's 1848 theory stating that diseases arise from cellular abnormalities, marking the start of modern medicine.

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    Cellular Pharmacology

    Ehrlich's 1899 work that began the field of pharmacology focusing on cellular interactions with drugs.

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    Hydrogen atom size

    The diameter of a hydrogen atom is 0.1 nm.

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    DNA diameter

    The diameter of DNA is 2 nm.

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    Nanoscale behavior

    At nanoscale, intermolecular forces and surface tension dominate instead of gravity.

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    Resonance frequency

    Smaller biological objects have larger resonance frequencies due to size inversely related to wavelength.

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    Eukaryotic cell size

    The average size of a eukaryotic cell is 10,000 nm (10 μm).

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    Endoplasmic Reticulum (ER)

    A network of membranes in eukaryotic cells involved in protein and lipid synthesis, as well as transport.

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    Granular ER

    Part of the endoplasmic reticulum involved in protein synthesis, characterized by ribosomes on its surface.

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    Agranular ER

    Part of the endoplasmic reticulum involved in lipid synthesis without ribosomes on its surface.

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    Golgi Apparatus

    Cell organelle that processes, sorts, and packages proteins and lipids for transport.

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    Transport Vesicles

    Membrane-bound sacs that carry proteins or lipids from the ER to the Golgi apparatus.

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    Mitochondria

    Organelles known as the powerhouses of the cell, involved in energy transformation and cellular respiration.

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    Chloroplast

    Plant organelles that conduct photosynthesis; theorized to have originated from bacteria.

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    Endosymbiotic Theory

    The theory proposing that mitochondria and chloroplasts originated from free-living prokaryotes that became symbiotic within eukaryotic cells.

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    E. coli

    A common bacterium found in the human intestine.

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    Prokaryotic cell structure

    E. coli has a rigid cell wall and a plasma membrane.

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    Cell wall composition

    E. coli's cell wall is made of polysaccharides and peptides.

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    Plasma membrane

    The plasma membrane is a selective phospholipid bilayer.

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    Eukaryotes complexity

    Eukaryotes have complex intracellular compartmentalization.

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    Yeasts

    Yeasts are simple unicellular eukaryotes more complex than bacteria.

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    Amoeba proteus

    A large, complex unicellular eukaryote capable of various tasks.

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    Evolutionary biology challenge

    The origin of eukaryotes presents a major challenge in evolution.

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    Primitive Atmosphere

    The early atmosphere of Earth, thought to have minimal free oxygen, creating reducing conditions.

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    Miller's Experiment

    1950s experiments that showed organic molecules could form from simple gases using electrical sparks.

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    Formation of Macromolecules

    Monomers like amino acids polymerizing spontaneously under prebiotic conditions to form polymers.

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    RNA World Hypothesis

    The theory that self-replicating RNA molecules were the first genetic systems in early life.

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    Prokaryotic Evolution

    The evolutionary development of prokaryotes that lasted 1 to 1.5 billion years.

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    Archaebacteria

    A group of prokaryotes that thrive in extreme environments, such as hot sulfur springs.

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    Eubacteria

    The other group of prokaryotes distinct from archaebacteria, often found in more common environments.

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    Self-Replication

    The ability of macromolecules like nucleic acids to replicate themselves, vital for life.

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    Common Ancestor

    The early life form from which present-day archaebacteria, eubacteria, and eukaryotes descended.

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    Endosymbiosis

    Theory that explains the origin of mitochondria and chloroplasts from bacteria through symbiotic relationships.

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    Organelle DNA

    DNA contained within mitochondria and chloroplasts, distinct from nuclear DNA.

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    Evolution of ATP

    ATP synthesis evolved in three stages: glycolysis, photosynthesis, and oxidative phosphorylation.

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    Viral origin hypothesis

    Theory suggesting the nucleus evolved from an RNA virus through gene acquisition from hosts.

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    Adenosine triphosphate (ATP)

    Major direct source of metabolic energy for cellular processes.

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    Photosynthesis

    Process by which chloroplasts convert sunlight into chemical energy.

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    Oxidative phosphorylation

    A metabolic pathway that uses oxygen to produce ATP, occurring in mitochondria.

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    Aerobic Bacteria

    Bacteria that need oxygen for survival, believed to be the ancestors of mitochondria.

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    Study Notes

    Modern Physiology

    • Physiology is an academic discipline
    • Modern physiology is a synthetic discipline, including classical, cellular, and molecular biology
    • Physiology, cellular physiology, and pharmacology are closely related

    Cell as the Basic Unit of Life

    • Evolution of the cell concept:
      • 1665: Robert Hooke introduced the concept of the cell
      • 1670s: Antoine van Leeuwenhoek made biological observations using a microscope
      • 1833: Robert Brown discovered the nucleus
      • 1838: Schleiden and Schwann developed the Cell Theory, stating all organisms are composed of cells
    • Cellular Theory (1838): marks the beginning of modern biology.
    • Cellular Pathology (1848): marks the beginning of modern medicine.
    • Cellular Pharmacology (1899): marks the beginning of pharmacology.

    Basic Cell Composition

    • Cells mainly consist of carbon, oxygen, hydrogen, nitrogen, calcium, and phosphorus.
    • These elements make up 99% of cell weight.
    • Carbon, hydrogen, oxygen, and nitrogen account for 96%.
    • The other 5 elements (sodium, potassium, chlorine, magnesium, and sulfur) amount to 0.85%
    • 11 mentioned elements are crucial for life; the remaining elements are trace elements.

    Cell Types

    • Cells are categorized based on whether they contain a nucleus.
    • Prokaryotes: Single-celled organisms without a nucleus (bacteria, 1-5 μm).
    • Eukaryotes: Organisms with a nucleus (plants, animals, fungi, algae, protozoa, 10-100 μm). More complex and larger.
    • New biological classification divides life into three domains: Bacteria, Archaea, and Eukaryotes.

    Surface Area/Volume Ratio

    • Surface area/volume ratio is a major constraint of cell size.
    • Larger surface area (relative to volume) is crucial for efficient transport and metabolism.
    • Cell volume increases faster than surface area.

    Homeostasis

    • The internal environment must remain relatively constant for independent and functional life
    • This is vital for all organ functions across organisms.
    • Homeostasis is maintained by organ systems, aiming to maintain stable internal physiology

    Cell Volume and Pressure

    • Various molecules, from hydrogen to large eukaryotic cells, have dimensions and volumes expressed at the nanometer scale.

    Elements of Cellular Evolution: Primitive Atmosphere

    • Simple organic molecules emerged from the early Earth’s primitive atmosphere
    • These molecules polymerized due to low PO2 in the primitive atmosphere.
    • Sun's energy in the form of radiant or electrical energy aided in the formation of organic molecules.

    Elements of Cellular Evolution: Formation of Organic Molecules

    • Miller's experiments replicated early Earth conditions and demonstrated the spontaneous synthesis of organic molecules.

    Elements of Cellular Evolution: Macromolecules and the RNA World

    • Monomers polymerize to form macromolecules. This process is driven by prebiotic conditions.
    • Nucleic acids (RNA) have unique capabilities for replicating their structures, crucial for self-replication and reproduction.
    • RNA molecules are self-replicating catalytic entities crucial for the evolution of life (RNA World Hypothesis).

    Evolution of Prokaryotes and Eukaryotes

    • Prokaryotic life predates eukaryotic life, lasting approximately 1-1.5 billion years.
    • Eukaryotic life evolved significantly later (approximately 2.7 billion years ago).
    • Bacteria/Archea are different from each other. The divergence occurred early in the evolution of life.

    Elements of Evolution: Prokaryotic Cells

    • Present-day prokaryotes fall into two categories: Archaebacteria and Eubacteria.
    • Archaebacteria are often adapted to harsh environments.
    • Prokaryotic cells are generally smaller and simpler (without internal membrane-bound organelles).
    • Prokaryotic cells have less complex genomes.
    • All present-day cells, prokaryotes and eukaryotes, originate from a common ancestral cell.

    Prokaryotes

    • Bacteria are a vast group that inhabit various environments (soil, water, organisms).
    • Bacteria have a wide variety of forms (spherical, rod-shaped, spiral), ranging in diameter from 1 to 10 μm.
    • Prokaryotes have relatively small genomes (0.6 to 5 million base pairs coding for about 5000 proteins)
    • Genetic material is not contained in a membrane-bound nucleus.

    Elements of Evolution: Eukaryotic Cells

    • The defining feature of eukaryotes is the presence of membrane-bound organelles.
    • Eukaryotic cells are larger and more complex, possessing membrane-bound organelles and a cytoskeleton lacking in Prokaryotes.
    • Eukaryotic cells have a more extensive compartmentalization compared to prokaryotes.

    Yeast as an Example of Eukaryotes

    • Yeasts are simple, unicellular eukaryotes. They exhibit self-replication capabilities.
    • Yeasts are simpler and smaller than cells in animals and plants.

    Amoeba as an Example of Eukaryotes

    • Amoeba (one example of a unicellular eukaryote) are complex cells.
    • Amoeba exhibit a variety of tasks (photosynthesis, movement) and can ingest other organisms.
    • Amoeba are highly mobile organisms using cytoplasmic extension to engulf other organisms.

    The Last Eukaryote Common Ancestor (LECA)

    • All present-day eukaryotic species arose from a single common ancestor.
    • Conservation of many genes throughout eukaryotic evolution suggests the fundamental features of cellular processes arose early in eukaryotic evolution.
    • Eukaryotic cells have a more complex and highly organized architecture.

    Eukaryotic Cells and the Kinase-Phosphatase Regulatory System

    • The complex architecture of eukaryotic cells is supported by advanced signalling mechanisms.
    • A critical process are phosphorylation and dephosphorylation reactions, catalyzed by enzymes called kinases and phosphatases, respectively.

    Nucleus

    • The nucleus is the central organelle of eukaryotic cells, containing genetic material.
    • Nucleus is surrounded by a double membrane called the nuclear envelope.
    • Nuclear pores allow selective transport between the nucleoplasm (nucleus contents) and the cytoplasm.

    Organelles

    • Endoplasmic Reticulum (ER): Involved in protein synthesis, lipid synthesis, membrane production, and xenobiotic metabolism.
    • Golgi Complex: Processes and packages proteins, sorting for delivery to their final destinations.
    • Ribosomes: Sites of protein synthesis.
    • Lysosomes and Peroxisomes: Membrane-bound organelles that degrade waste products and molecules.
    • Mitochondria: Powerhouse of the cell, producing ATP through cellular respiration.
    • Chloroplasts: Organelle responsible for photosynthesis (only in plants).
    • Cytoskeletal elements: Provide structural support.
    • Nuclear Envelope: Double membrane surrounding the nucleus
    • Nuclear Pores: Allow selective transport between the nucleus and cytoplasm.

    Mitochondria and Chloroplasts

    • Mitochondria and chloroplasts are thought to have arisen through endosymbiosis, involving a symbiotic relationship with bacterial precursors.
    • Mitochondria (in most eukaryotes) are sites of oxidative metabolism and ATP production.
    • Chloroplasts (found in plant cells) are the site of photosynthesis.
    • Both mitochondria and chloroplasts have their own DNA and ribosomes, further supporting their endosymbiotic origin.

    Nucleus - Viral Origin Hypothesis

    • A hypothesis that suggests the nucleus evolved from a DNA virus.
    • According to this hypothesis, the virus gradually acquired genes from the host genome over time and eventually integrated into the host cell structure becoming a nucleus.

    Evolution of Metabolism

    • Controlled metabolic energy utilization is central to all cellular activity.
    • Adenosine triphosphate (ATP) is the primary energy currency for cells.
    • Three evolutionary steps are associated with the development of ATP synthesis: glycolysis, photosynthesis, and oxidative phosphorylation.

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