Biological Research Techniques and Safety

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A tool used in research to grow and study eukaryotic or prokaryotic cells in controlled conditions.

Cell lines are a benefit in scientific research because they are homogenous and produce reproducible data.

Safety for both lab personnel and cultured cells is paramount. This includes preventing contamination from microorganisms.

The selection of a cell line and specific culture conditions depends on the research question.

Techniques like cell propagation are essential for manipulating cells in various experiments. These techniques can be adapted for many cell types.

The practice of growing and maintaining cells in a controlled laboratory environment. Commonly used to study cell biology, disease mechanisms, and drug toxicity.

A group of cells derived from a single ancestor and therefore genetically identical, often used in cell culture to ensure consistency.

A system that ensures the absence of harmful microorganisms, essential for successful cell culture to prevent contamination.

The use of protective measures to reduce or eliminate exposure to hazardous agents in a laboratory setting.

Personal protective equipment, such as lab coats, gloves, and goggles, that act as a barrier between the researcher and potential hazards.

A type of safety cabinet that provides a sterile workspace, minimizing contamination of both the researcher and the cell cultures.

A document that provides detailed information about a chemical or substance, including its hazards, storage, disposal, and safety precautions.

A standardized set of practices designed to minimize the risk of infection and contamination in cell culture laboratories.

The introduction of a potentially hazardous agent into the body through contact with contaminated needles, syringes, or other sharps.

The entry of hazardous substances into the body through swallowing something contaminated, such as food or drink.

The inhalation of airborne particles or aerosols containing potentially hazardous substances.

Direct contact of potentially hazardous substances with skin or mucous membranes, like those in the eyes, nose, or mouth.

The disposal of infectious or hazardous materials following established guidelines to prevent contamination.

The intentional introduction of genetically altered or modified cells into a living organism, with the goal of treating or preventing disease.

A type of cell culture that involves the cultivation of cells derived directly from living tissues, often used for research and diagnostics.

A cleaning process that uses high heat and pressure to kill microorganisms.

A method of sterilization that involves passing a liquid through a filter with pores small enough to trap microbes.

Chemicals used in cell culture media to inhibit the growth of bacteria.

A common bacterial species that can contaminate cell cultures, often appearing as small particles under a microscope.

A fungal contaminant that can appear as multicellular, highly connected, thin filaments under a microscope.

A fungal contaminant that forms multicellular stringlike structures with ovoid shapes under a microscope.

A type of cell culture contamination that occurs when other cell types are introduced into the culture, leading to a heterogeneous mix.

The introduction of undesirable chemical impurities into cell culture, such as plasticizers from cell culture vessels.

The process of visually inspecting cell cultures under a microscope to detect signs of contamination.

Turbidity in cell culture media, indicating the presence of microbial contaminants that are multiplying.

A color change in phenol red, an indicator in cell culture media, towards yellow, indicating a change in pH due to microbial contamination.

A group of cells grown in a lab that are descended from a single ancestral cell, often genetically identical.

The process of introducing infectious agents, like bacteria, into a sterile environment.