Biological Research Techniques and Safety

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<p>Cell lines offer homogeneity and reproducibility in the data generated.</p> Signup and view all the answers

<p>Safety guidelines for lab personnel and the cultured cells, as well as contamination prevention.</p> Signup and view all the answers

<p>Contaminants can be avoided through proper lab practices and detected early using specific techniques.</p> Signup and view all the answers

<p>The choice depends on the readout and requirements of the desired assay.</p> Signup and view all the answers

<p>Cell culture refers to laboratory methods that enable the growth of eukaryotic or prokaryotic cells in physiological conditions.</p> Signup and view all the answers

<p>Cell culture techniques were initially used to study tissue growth, virus biology, vaccine development, and the role of genes in health and disease.</p> Signup and view all the answers

<p>Cell culture allows for homogeneity in clonal populations and the control of environmental variables, leading to high reproducibility and consistency.</p> Signup and view all the answers

<p>Precautions include wearing personal protective equipment, decontaminating lab tools, and following disposal protocols for hazardous materials.</p> Signup and view all the answers

<p>PPE serves as a barrier to protect laboratory workers from hazardous agents and prevent contamination of cell cultures.</p> Signup and view all the answers

<p>Asepsis refers to creating a microenvironment free of unwanted pathogenic microorganisms to maintain successful cell culture.</p> Signup and view all the answers

<p>Biosafety cabinets provide a controlled environment with HEPA-filtered air to minimize exposure to hazardous materials and protect cultured cells from contamination.</p> Signup and view all the answers

<p>Cultured cells can harbor infectious agents like HBV or HIV, alongside toxic and corrosive reagents.</p> Signup and view all the answers

<p>Personnel can minimize risks by undergoing stringent training, implementing standard safety practices, and following proper protocols for handling hazardous agents.</p> Signup and view all the answers

<p>Screening donors is essential to identify and exclude any disease-causing pathogens that could contaminate the primary cells.</p> Signup and view all the answers

<p>The laboratory's safety officer should be notified immediately to advise on an appropriate containment and decontamination strategy.</p> Signup and view all the answers

<p>The MSDS provides detailed information regarding chemical safety, including potential health hazards, toxicity, and safe handling practices.</p> Signup and view all the answers

<p>Common practices include dedicating a separate area for cell culture work and using equipment that promotes a sterile workspace.</p> Signup and view all the answers

<p>Removing contaminated gloves immediately prevents the spread of hazardous agents and minimizes exposure risks.</p> Signup and view all the answers

<p>Food and drink consumption, as well as storage, is strictly prohibited in cell culture labs to prevent contamination.</p> Signup and view all the answers

<p>Laboratory staff, the environment, and culture medium are the primary sources of contamination.</p> Signup and view all the answers

<p>Microbial contaminants can alter the phenotype and genotype of cultured cells by competing for nutrients and producing toxic by-products.</p> Signup and view all the answers

<p>The biosafety cabinet restricts nonsterile aerosols and airborne particles, protecting cultured cells from contamination.</p> Signup and view all the answers

<p>The work surface should be decontaminated with an antifungal detergent followed by 70% ethanol.</p> Signup and view all the answers

<p>Regular maintenance ensures correct airflow and full filter capacity, which are crucial for preventing contamination.</p> Signup and view all the answers

<p>Lab coats should only be worn within the cell culture lab and washed regularly at hot temperatures.</p> Signup and view all the answers

<p>Antibiotics limit the risk of bacterial growth in media bottles and cell culture vessels after opening.</p> Signup and view all the answers

<p>Filter-sterilization is done by forcing liquid through a 0.22 μM filter using a vacuum pump.</p> Signup and view all the answers

<p>Items should be sprayed and wiped with 70% ethanol before entering the biosafety cabinet.</p> Signup and view all the answers

<p>Bacterial contamination can increase the metabolic rates, modifying the pH of the culture media and changing phenol red's color to yellow.</p> Signup and view all the answers

<p>Minimizing items prevents obstruction of airflow, which is essential for maintaining a sterile environment.</p> Signup and view all the answers

<p>Training staff helps in identifying early signs of contamination to prevent its spread to other cultures.</p> Signup and view all the answers

<p>Laboratory staff should wash their hands with soap and wear disposable gloves sprayed with 70% ethanol.</p> Signup and view all the answers

<p>Keeping the area free from dust helps minimize the risk of microbial contamination.</p> Signup and view all the answers