Biological Oxidation: Aerobic and Anaerobic

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Questions and Answers

Which of the following best describes biological oxidation?

  • The reduction of energy-rich substances to store energy.
  • The breakdown of water into hydrogen and oxygen.
  • The oxidation of energy-rich substances to produce energy. (correct)
  • The synthesis of complex molecules from simple substances.

During biological oxidation, what particles are removed from oxidizing substances?

  • Neutrons
  • Protons (H+) and electrons (e-) (correct)
  • Alpha particles
  • Water molecules

What typically accompanies oxidation reactions in biological systems?

  • Neutralization reactions
  • Hydrolysis reactions
  • Reduction reactions (correct)
  • Polymerization reactions

What is the direct molecule that can immediately release energy in cells?

<p>ATP (D)</p> Signup and view all the answers

Which statement accurately describes 'redox' reactions?

<p>Redox reactions are termed as 'oxidation-reduction' reactions. (D)</p> Signup and view all the answers

What is the role of carrier molecules in biological oxidation?

<p>To initially transfer H-atoms (B)</p> Signup and view all the answers

What must occur for glycolysis to continue functioning?

<p>NADH must be oxidized to NAD+. (B)</p> Signup and view all the answers

Where are all components of the electron transport chain (ETC) located?

<p>The inner mitochondrial membrane (D)</p> Signup and view all the answers

Which best describes the role of NADH and FADH2 in the electron transport chain?

<p>They reform coenzymes and pass through the electron transport chain. (C)</p> Signup and view all the answers

What role do cytochromes play in the electron transport chain?

<p>They facilitate electron transfer due to the presence of iron (Fe) ions. (D)</p> Signup and view all the answers

Which of the following represents the correct sequence of the three stages of food oxidation?

<p>Digestion, TCA Cycle, ETC (D)</p> Signup and view all the answers

In anaerobic oxidation, what is the fate of H+ and e-?

<p>They are transported to other substrates and reduced. (B)</p> Signup and view all the answers

Which statement correctly describes the structure of mitochondria?

<p>The inner membrane is impermeable to most small ions. (C)</p> Signup and view all the answers

How is energy derived in oxidative phosphorylation?

<p>From a proton (H+) gradient. (A)</p> Signup and view all the answers

Which process MUST occur in the presence of oxygen?

<p>Oxidative phosphorylation (D)</p> Signup and view all the answers

What is the primary function of Superoxide Dismutase (SOD)?

<p>To convert superoxide anions into hydrogen peroxide (A)</p> Signup and view all the answers

What is the role of 'reductant' in redox reactions?

<p>The electron donor (C)</p> Signup and view all the answers

Which of the following is a characteristic true about endergonic reactions?

<p>Energy released is greater than the energy input. (C)</p> Signup and view all the answers

Which statement regarding anaerobic oxidation is correct?

<p>It involves the transportation of H+ and e- to another substrates and they are reduced (A)</p> Signup and view all the answers

How does the inner mitochondrial membrane's structure contribute to its function?

<p>Its convolutions increase surface area, enhancing ATP production. (D)</p> Signup and view all the answers

Given that both oxidative and substrate-level phosphorylation produce ATP, what most clearly distinguishes oxidative phosphorylation?

<p>Oxidative phosphorylation uses ATP synthase and a proton gradient. (C)</p> Signup and view all the answers

Which statement regarding the fate of pyruvate is most accurate?

<p>With oxygen, pyruvate is oxidized to acetyl CoA for the Krebs cycle. (C)</p> Signup and view all the answers

NAD+, NADP+, and FAD cannot be synthesized in the body so must be supplied in the diet. These are all complex molecules containing which component?

<p>Vitamins (D)</p> Signup and view all the answers

In biological systems, what is the MAIN source of biological energy in the cellular process?

<p>Oxidative-reductive reactions (C)</p> Signup and view all the answers

Which of the following statements describes biological oxidation?

<p>Biological oxidation is the removal of electrons. (A)</p> Signup and view all the answers

What is the role of mobile electron carriers such as Coenzyme Q and Cytochrome c in the electron transport chain (ETC)?

<p>To transport electrons between protein complexes (D)</p> Signup and view all the answers

Which stage accounts for almost 90% of the ATP generated by cellular respiration?

<p>Oxidative phosphorylation (D)</p> Signup and view all the answers

If a researcher experimentally removes Complex V from the electron transport chain, what direct effect would be observed?

<p>Impairment in ATP production (B)</p> Signup and view all the answers

Cells release energy from fuel molecules through oxidation reactions; what three things occur during these reactions (select three)?

<p>Removal of H-atoms (A), Removal of electrons (B), Addition of oxygen (C)</p> Signup and view all the answers

All oxidation reactions are accompanied by reduction reactions. Which processes occur during reduction reactions (select three)?

<p>The addition of H atoms (A), The addition of electrons (B), The removal of oxygen (D)</p> Signup and view all the answers

What could best be described as the removal of electrons?

<p>Oxidation (D)</p> Signup and view all the answers

What could best be described as the gain of electrons?

<p>Reduction (D)</p> Signup and view all the answers

Which of the following enzymes requires molybdenum as an important component?

<p>Xanthine oxidase (B)</p> Signup and view all the answers

During biological oxidation, hydrogen atoms are transported with the help of Dehydrogenase enzymes. This process is otherwise known as?

<p>Hydrogen Transport (C)</p> Signup and view all the answers

What is the important function of Hydroperoxidases in biological oxidation?

<p>Protect body against the harmful effect of peroxides (A)</p> Signup and view all the answers

Which of these reactions apply in the process of catalytic action of Dioxygenases?

<p>A+O2-&gt;AO2 (C)</p> Signup and view all the answers

Which of these enzymes is located mainly in the endoplasmic reticulum of liver & intestine but also in the mitochondria?

<p>Cytochrome P450 (A)</p> Signup and view all the answers

Certain substances called uncouplers can disrupt the essential linkage between electron transport and ATP synthesis. By what means do they achieve this?

<p>Dissolving in the mitochondrial membrane and acting as carriers for H+ (C)</p> Signup and view all the answers

Cytochrome oxidase is inhibited by several substances; select any three of these from the options below:

<p>Hydrogen Sulfide (B), Cyanide (C), Carbon Monoxide (D)</p> Signup and view all the answers

Oligomycin is an inhibitor of oxidative phosphorylation. What specific process does it inhibit?

<p>ATP Synthase (C)</p> Signup and view all the answers

Which complexes are part of the mobile electron carriers within the electron transport chain?

<p>Coenzyme Q and Cytochrome c (B)</p> Signup and view all the answers

What is the classification of enzymes involved in oxidation & reduction in the human body?

<p>Oxidoreductases (D)</p> Signup and view all the answers

ATP molecules are the basic transporters of cellular energy. What is the approximate energy released by one molecule of ATP?

<p>-7.3 kcal/mol (A)</p> Signup and view all the answers

Valiomycin, an ionophore, is a:

<p>Forms a lipid complex through which the K+ ion readily pass through (C)</p> Signup and view all the answers

A biochemist is studying a new enzyme and finds that it catalyzes the incorporation of one atom of molecular oxygen ($O_2$) into a substrate, with the other oxygen atom being reduced to water. Which class of enzymes does this enzyme most likely belong to?

<p>Monooxygenases (C)</p> Signup and view all the answers

What is the primary role of NAD+ and FAD in biological oxidation?

<p>To serve as carrier molecules for hydrogen atoms and electrons. (B)</p> Signup and view all the answers

During aerobic oxidation, what is the final acceptor of hydrogen ions and electrons?

<p>Oxygen ($O_2$). (D)</p> Signup and view all the answers

Which of the following is a characteristic feature of the inner mitochondrial membrane?

<p>Impermeable to most ions and small molecules, requiring specialized carriers. (A)</p> Signup and view all the answers

What role do the cristae play within the mitochondria?

<p>They greatly increase the surface area of the inner mitochondrial membrane. (C)</p> Signup and view all the answers

Which statement accurately describes the location of the electron transport chain (ETC) components?

<p>They are located on the inner mitochondrial membrane. (C)</p> Signup and view all the answers

How do uncouplers affect oxidative phosphorylation?

<p>By dissipating the proton gradient across the inner mitochondrial membrane. (A)</p> Signup and view all the answers

Which complex does Coenzyme Q occupy position in the electron transport chain?

<p>Between metalloflavoproteins and cytochrome. (B)</p> Signup and view all the answers

Which class of enzymes catalyzes the direct incorporation of oxygen into a substrate molecule?

<p>Oxygenases. (A)</p> Signup and view all the answers

What is the role of cytochrome oxidase within the electron transport chain?

<p>To catalyze the final transfer of electrons to oxygen. (A)</p> Signup and view all the answers

What characteristic defines a 'reductant' in a redox reaction?

<p>It loses electrons and is oxidized. (B)</p> Signup and view all the answers

In the absence of oxygen, how is NADH recycled to NAD+ to allow glycolysis to continue?

<p>Through fermentation. (A)</p> Signup and view all the answers

Valiomycin acts primarily as:

<p>An ionophore. (C)</p> Signup and view all the answers

What is the function of Superoxide Dismutase (SOD) in biological systems?

<p>To convert superoxide radicals into hydrogen peroxide. (D)</p> Signup and view all the answers

What is the significance of heme in the context of cytochromes?

<p>It contains iron ions that undergo redox reactions.. (C)</p> Signup and view all the answers

Which of the following characteristics is associated with Cytochrome P450?

<p>It is a monooxygenase involved in detoxification reactions. (D)</p> Signup and view all the answers

If Complex V (ATP synthase) is experimentally removed from a mitochondrial membrane, what immediate effect would be observed?

<p>Cessation of ATP production. (A)</p> Signup and view all the answers

Under anaerobic conditions during intense exercise, muscle cells convert pyruvate to lactate. What is the primary reason for this conversion?

<p>To regenerate NAD+ allowing glycolysis to continue. (D)</p> Signup and view all the answers

How does carbon monoxide (CO) inhibit the electron transport chain, and what is the direct consequence?

<p>By inhibiting Complex IV, blocking the final electron transfer to oxygen. (B)</p> Signup and view all the answers

A researcher discovers a novel compound that, when added to actively respiring mitochondria, increases oxygen consumption but decreases ATP production. This compound is most likely acting as:

<p>An uncoupler. (B)</p> Signup and view all the answers

A biochemist is investigating a new enzyme and notices how it incoporates one atom of molecular oxygen ($O_2$) into a substrate, while reducing the other atom of oxygen to water ($H_2 O$). What class of enzymes could this likely be?

<p>Monooxygenases. (A)</p> Signup and view all the answers

Flashcards

Biological Oxidation

Oxidation of energy-rich substances like carbohydrates, lipids, and proteins in biological systems to produce energy.

Oxidation reactions

Reactions where cells release energy by removing electrons or hydrogen atoms, or by adding oxygen from fuel molecules.

Reduction reactions

Reactions that accompany oxidation, involving the addition of hydrogen atoms/electrons or the removal of oxygen.

NAD+, NADP+, and FAD

NAD+, NADP+, and FAD are complex molecules that contain components the body can't synthesize and must be obtained through diet (vitamins).

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Endergonic reactions

Reactions where the energy released is greater than the energy input.

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Exergonic reactions

Reactions where the energy input is greater than the energy released.

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ATP (Adenosine Triphosphate)

The direct molecule that can release energy immediately in cells.

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Biological oxidation (definition)

An energy-producing reaction in living cells coupled with a reduction reaction; the cellular process where organic substances release energy, producing CO2 and H2O.

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Oxidation (definition)

The removal of electrons from a substance.

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Reduction (definition)

The gain of electrons by a substance.

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Redox potential (E'0)

The free energy change that occurs in reactions involving oxidation and reduction.

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Biological oxidation

Series of processes organisms use to derive energy from nutrients and fuel molecules via redox reactions.

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Aerobic oxidation

The removal of electrons (or H-atoms) with oxygen as final acceptor.

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Reducing equivalents

The reducing equivalents transferred to NAD and FAD to produce NADH and FADH2, then through the electron transport chain to reduce oxygen to water.

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Electron Transport Chain (ETC)

Process where electrons are transported to O2 via special components (NAD, FMN, etc.) located on the inner mitochondrial membrane.

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ATP synthesis

Mechanism by which ATP is synthesized, involving a phosphate group added to ADP.

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Substrate-level phosphorylation

ATP is formed by transferring a phosphate directly from substrate molecules to ADP.

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Oxidative phosphorylation

An ATP formation process that occurs only in the presence of oxygen. It accounts for almost 90% of the ATP from cellular respiration.

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Fate of pyruvate

The process where, with oxygen present, pyruvate is oxidized to acetyl CoA, entering the Krebs cycle, or, without oxygen, pyruvate is reduced to oxidize NADH back to NAD+.

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Fermentation

A process that occurs when oxygen is unavailable, where an organic molecule is final electron acceptor; NADH must be recycled to NAD+.

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Mitochondria

The powerhouse of the cell, comprised of an outer membrane, inter-membrane space, and an impermeable inner membrane to most small ions.

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NAD+ and FAD (in matrix)

NAD+ and FAD, components of the matrix within the mitochondria, required as hydrogen acceptors.

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Oxidoreductases

Enzymes that catalyze oxidation-reduction reactions.

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Oxidases

Enzymes that catalyze oxidation of a metabolite by oxygen, forming water or hydrogen peroxide.

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Cytochrome oxidase

A hemoprotein widely distributed in tissues, inhibited by CO, cyanide, and hydrogen sulfide.

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Dehydrogenases

Enzymes that catalyze hydrogen transport, using nicotinamides or riboflavin as coenzymes.

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Hydroperoxidases

Enzymes that protect the body against harmful effects of peroxides.

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Peroxidases

Enzymes present in milk and various tissues, catalyzing reactions with peroxides.

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Catalase

A hemoprotein containing four heme groups, which catalyzes the breakdown of hydrogen peroxide.

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Oxygenases

Enzymes that catalyze the direct transfer and incorporation of oxygen into a substrate molecule.

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Dioxygenases

Enzymes that incorporate two atoms of molecular oxygen into a substrate.

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Cytochromes P450

Heme-containing monooxygenases located in the endoplasmic reticulum of liver and intestine, involved in detoxification.

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Superoxide Dismutase

Enzyme that transfers a single electron to O2, generating a superoxide anion free radical.

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ATP functions

The basic transporter of cellular energy used for mobility, membrane transport, signal transduction, and nucleotide synthesis.

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Oxidative Phosphorylation (definition)

ATP synthesis by the transport of electrons to molecular oxygen.

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Complex I (ETC)

Located in the inner mitochondrial membrane and also contains non heme iron atoms.

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Complex II (ETC)

Coenzyme Q (Q for Quinone) or cytochrome c reductase

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Complex III (ETC)

A protein with Cytochrome c oxidase

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Complex IV (ETC)

Protein that catalyses the transport of electrons from cytochrome c to molecular O2 and thus reduces O2 to H2O

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Complex V (ETC)

ATP synthase, has two components FO andF1.

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Inhibitors of Respiratory Chain

Inhibitors that are barbiturates like amobartital, antibiotic like piericidin A, antimycin A.

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Inhibitors of oxidative phosphorylation

Are oligomycin and atrctyloside, they interrupt ATP synthase

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Uncouplers of phosphorylation

Dissolve in the membrane, and function as carriers for H+ or it can be an ionophores.

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Ionophores

Lipid soluble substances capable of carrying specific ions through the membrane.

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Study Notes

  • Biological oxidation involves the oxidation of energy-rich chemical substances, such as carbohydrates, lipids, and proteins
  • Biological oxidation that occurs in biological systems produce energy.
  • Oxidizing substances remove protons (H+) and electrons (e-) during oxidation processes.
  • Protons and electrons are transported to acceptors using special transporters.
  • Transportation of H+ and e- to acceptors causes energy to be released, accumulating in the ATP molecule.

Biological Oxidation Types

  • There are two types of biological oxidation: anaerobic and aerobic.
  • Anaerobic oxidation involves the transportation of H+ and e- to other substrates (acceptors), causing their reduction.
  • In anaerobic glycolysis, H+ and e- are transported by NAD to pyruvate, reducing it to lactate, and releasing energy that accumulates in 2 ATP molecules.
  • Aerobic oxidation involves the transportation of H+ and e- to O2 (acceptor).
  • Energy-rich substances like carbohydrates, lipids, and proteins are oxidized to CO2 and H2O in aerobic oxidation.
  • The reducing equivalents, H+ and e-, from intermediate substances are transferred to NAD and FAD, forming NADH and FADH2.
  • The forms of coenzymes NADH and FADH2 pass through the electron transport chain (ETC) or respiratory chain, reducing oxygen to water.
  • The components are NAD, FMN, Iron-Sulfur protein, Coenzyme Q, Cytochromes b, c1, c2, a, a3.
  • The ETC is located on the inner mitochondrial membrane.

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