Biological Evidence in Forensic Science
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Questions and Answers

What kind of tests are fast, sensitive, and can be done at the scene to determine the presence of physiological fluid stain from a human?

  • Protein typing
  • Presumptive testing (correct)
  • Confirmatory testing
  • Species testing
  • Which type of testing allows for unequivocal identification of the biological material but may be less sensitive and more time-consuming?

  • Confirmatory testing
  • Species testing (correct)
  • Protein typing
  • Presumptive testing
  • What kind of markers can distinguish different animal species?

  • Confirmatory testing
  • Protein typing
  • Presumptive testing
  • DNA typing (correct)
  • In the context of biological evidence, what is needed to ask the right questions with regard to evidence analysis?

    <p>A scientist</p> Signup and view all the answers

    In forensic genealogy, what is GEDmatch used for?

    <p>To search for relatives using DNA data</p> Signup and view all the answers

    What is the significance of low copy number DNA in forensic analysis?

    <p>It requires more stringent amplification conditions</p> Signup and view all the answers

    What is the purpose of familial DNA testing in forensic investigations?

    <p>To find a relative whose DNA profile is in the CODIS database</p> Signup and view all the answers

    What can degrade DNA in the environment?

    <p>Time, temperature, humidity, light, and chemical/environmental hazards</p> Signup and view all the answers

    $f(x) = -4(x + 3)^2 + 2$. What does this equation represent?

    <p>$f(x)$ represents a parabola that opens downwards with vertex $(-3, 2)$.</p> Signup and view all the answers

    $g(x) = rac{1}{x}$. What type of function is $g(x)$?

    <p>$g(x)$ represents a reciprocal function.</p> Signup and view all the answers

    What can cause false positive results in DNA testing?

    <p>Matching at multiple loci without considering other factors</p> Signup and view all the answers

    What type of alleles are detected in STR analysis?

    <p>Short Tandem Repeat (STR) alleles</p> Signup and view all the answers

    What was the significance of the UK Burglary Suspect case mentioned in the text?

    <p>The suspect was falsely identified based on false positive DNA testing results</p> Signup and view all the answers

    What is the purpose of substrate control in forensic investigations?

    <p>To ensure that contaminants from the environment do not interfere with analysis results</p> Signup and view all the answers

    What does mitochondrial DNA (mtDNA) analysis help identify?

    <p>Maternal lineage and identification of individuals</p> Signup and view all the answers

    What can be uploaded to GEDmatch for forensic genealogy purposes?

    <p>DNA data from 23andMe and Ancestry.com</p> Signup and view all the answers

    Which method is well developed for forensic analysis of blood?

    <p>Morphological characteristics and DNA analysis</p> Signup and view all the answers

    What contributes to the uniqueness of individuals and their biological materials?

    <p>Polymorphisms in proteins, nucleic acids, and polysaccharides</p> Signup and view all the answers

    What percentage of the population is classified as secretors?

    <p>Around 80%</p> Signup and view all the answers

    What is correlated with secretor status and impacts the analysis of mixed stains in sexual assault cases?

    <p>$ABO$ agglutinogens presence</p> Signup and view all the answers

    What is crucial for accurate interpretation in DNA analysis?

    <p>Sensitivity and specificity</p> Signup and view all the answers

    What may offer a potential solution for distinguishing identical twins using current forensic DNA STR typing methods?

    <p>Epigenomics</p> Signup and view all the answers

    What allows for individualization based on DNA?

    <p>DNA sequences in chromosomes</p> Signup and view all the answers

    What are common sources of DNA at crime scenes?

    <p>Blood, semen, saliva, urine, hair, teeth, bone, tissue only</p> Signup and view all the answers

    What emphasizes the importance of proper collection and handling protocols for biological evidence?

    <p>The susceptibility of biological evidence to contamination or destruction</p> Signup and view all the answers

    Which type of testing is fast, sensitive, and can be done at the scene to determine the presence of physiological fluid stain from a human?

    <p>Molecular testing</p> Signup and view all the answers

    What kind of markers can distinguish different animal species?

    <p>Mitochondrial DNA markers</p> Signup and view all the answers

    What emphasizes the importance of proper collection and handling protocols for biological evidence?

    <p>The uniqueness of biological materials</p> Signup and view all the answers

    What is crucial for accurate interpretation in DNA analysis?

    <p>$STR$ analysis</p> Signup and view all the answers

    What is the purpose of forensic DNA analysis?

    <p>To identify the source of biological evidence</p> Signup and view all the answers

    What is the impact of secretor status on body fluids?

    <p>It affects the presence of ABO agglutinogens</p> Signup and view all the answers

    Which technique may offer a potential solution for distinguishing identical twins using current forensic DNA STR typing methods?

    <p>Epigenomics</p> Signup and view all the answers

    What is the most common source of DNA at crime scenes?

    <p>Blood</p> Signup and view all the answers

    $f(x) = -4(x + 3)^2 + 2$. What does this equation represent?

    <p>Quadratic function</p> Signup and view all the answers

    $g(x) = \frac{1}{x}$. What type of function is $g(x)$?

    <p>Rational function</p> Signup and view all the answers

    What is the significance of low copy number DNA in forensic analysis?

    <p>It refers to the small amount of DNA that can be used for analysis, which is crucial in cases where only a minute sample is available.</p> Signup and view all the answers

    What emphasizes the importance of proper collection and handling protocols for biological evidence?

    <p>The risk of contamination and degradation of biological samples due to environmental factors.</p> Signup and view all the answers

    What contributes to the uniqueness of individuals and their biological materials?

    <p>The variability in DNA profiles, including low copy number DNA.</p> Signup and view all the answers

    What can cause false positive results in DNA testing?

    <p>Secondary transfer of DNA from one surface to another.</p> Signup and view all the answers

    $g(x) = \rac{1},{x}$. What type of function is $g(x)$?

    <p>$g(x)$ represents a reciprocal function.</p> Signup and view all the answers

    $f(x) = -4(x + 3)^2 + 2$. What does this equation represent?

    <p>$f(x)$ represents a quadratic function with a vertex at $(-3, 2)$.</p> Signup and view all the answers

    What is the ideal environment for preservation of biological evidence?

    <p>Dry and frozen</p> Signup and view all the answers

    What is the significance of familial DNA testing in forensic investigations?

    <p>It helps in identifying potential relatives of the DNA source when the DNA profile is not in CODIS</p> Signup and view all the answers

    What does low copy number DNA refer to in forensic analysis?

    <p>DNA profiles obtained from a few skin cells</p> Signup and view all the answers

    What does GEDmatch, mentioned in the text, serve as a database for?

    <p>Forensic genealogy purposes</p> Signup and view all the answers

    What kind of markers are detected in STR analysis for forensic DNA profiling?

    <p>Autosomal markers</p> Signup and view all the answers

    What was the significance of the UK Burglary Suspect case mentioned in the text?

    <p>It raised concerns about false positive results in DNA testing</p> Signup and view all the answers

    What can cause false negative results in DNA testing?

    <p>Presence of multiple offenders</p> Signup and view all the answers

    What was used to identify Tsarina and 3 daughters, as mentioned in the LA Times Article?

    <p>mtDNA analysis</p> Signup and view all the answers

    What does mitochondrial DNA (mtDNA) analysis help identify?

    <p>Maternal lineage</p> Signup and view all the answers

    Which factor can degrade DNA in the environment according to the text?

    <p>Light and microorganisms</p> Signup and view all the answers

    What emphasizes the importance of proper collection and handling protocols for biological evidence?

    <p>Documentation of visual and written evidence</p> Signup and view all the answers

    Biological specificity screening tests are less sensitive and more time-consuming than confirmatory tests.

    <p>False</p> Signup and view all the answers

    Mitochondrial DNA analysis is used to identify the source of physiological fluid stains from humans.

    <p>False</p> Signup and view all the answers

    Forensic DNA analysis is always approached with the 'big picture' in mind, considering the totality of the physical evidence.

    <p>True</p> Signup and view all the answers

    Familial DNA testing in forensic investigations can cause false positive results in DNA testing.

    <p>True</p> Signup and view all the answers

    DNA analysis can definitively identify the source of biological materials at a crime scene.

    <p>False</p> Signup and view all the answers

    Contamination of biological samples can be completely avoided by using gloves and proper packaging.

    <p>False</p> Signup and view all the answers

    Forensic genealogy involves searching for DNA sources in the CODIS database.

    <p>False</p> Signup and view all the answers

    Low copy number DNA analysis is not applicable in forensic investigations due to its destructive nature.

    <p>False</p> Signup and view all the answers

    Mitochondrial DNA (mtDNA) analysis can help identify the physical appearance of the DNA source.

    <p>False</p> Signup and view all the answers

    The alleles detected in STR analysis for forensic DNA profiling are not unique to individuals.

    <p>False</p> Signup and view all the answers

    False positive results in DNA testing can occur due to the presence of advanced Parkinson's disease.

    <p>False</p> Signup and view all the answers

    The UK Burglary Suspect case demonstrated the potential for false negatives in DNA testing.

    <p>True</p> Signup and view all the answers

    Forensic DNA analysis can supplant other areas of evidential analysis in forensic science.

    <p>False</p> Signup and view all the answers

    GEDmatch is a database used for forensic genealogy purposes.

    <p>True</p> Signup and view all the answers

    Mitochondrial DNA (mtDNA) analysis can definitively identify the source of biological materials at a crime scene.

    <p>False</p> Signup and view all the answers

    Familial DNA testing in forensic investigations involves looking for a relative whose DNA profile is already in the CODIS database.

    <p>False</p> Signup and view all the answers

    Forensic DNA analysis seeks to identify the source of biological evidence and may employ techniques such as electrophoresis, ELISA, and DNA/RNA typing.

    <p>True</p> Signup and view all the answers

    Identical twins can be distinguished using current forensic DNA STR typing methods.

    <p>False</p> Signup and view all the answers

    Polymorphisms in proteins, nucleic acids, and polysaccharides do not contribute to the uniqueness of individuals and their biological materials.

    <p>False</p> Signup and view all the answers

    The presence of ABO agglutinogens in body fluids is not affected by secretor status.

    <p>False</p> Signup and view all the answers

    Every human being is biologically unique due to the different sequences of DNA in their chromosomes.

    <p>True</p> Signup and view all the answers

    Biological evidence can easily be contaminated or destroyed, emphasizing the unimportance of proper collection and handling protocols.

    <p>False</p> Signup and view all the answers

    Methods of analysis for blood are well developed for forensic purposes, while methods for other biological fluids do not vary in sophistication.

    <p>False</p> Signup and view all the answers

    DNA analysis cannot link a suspect to a crime scene or victim.

    <p>False</p> Signup and view all the answers

    Secretor status is not correlated with the Lewis system and does not impact the analysis of mixed stains in sexual assault cases.

    <p>False</p> Signup and view all the answers

    Semen is not commonly encountered in crimes of assault, homicide, and sexual assault.

    <p>False</p> Signup and view all the answers

    Forensic analysis for physiological fluids and DNA does not play a crucial role in crimes of assault, homicide, and sexual assault.

    <p>False</p> Signup and view all the answers

    Morphological characteristics and DNA analysis are not used for unequivocal identification of physiological fluids.

    <p>False</p> Signup and view all the answers

    Match the physiological fluid with its method of analysis:

    <p>Blood = Well-developed methods for forensic purposes Saliva = Varied in sophistication for forensic purposes Urine = Varied in sophistication for forensic purposes Semen = Varied in sophistication for forensic purposes</p> Signup and view all the answers

    Match the biological material with its impact on DNA analysis:

    <p>Secretor status = Affects the presence of ABO agglutinogens in body fluids Lewis system = Impacts the analysis of mixed stains in sexual assault cases Nuclear DNA = Used for unequivocal identification of physiological fluids Mitochondrial DNA = Used to identify the source of physiological fluid stains from humans</p> Signup and view all the answers

    Match the DNA analysis technique with its purpose:

    <p>Electrophoresis = Employed for identifying the source of biological evidence ELISA = Employed for identifying the source of biological evidence DNA/RNA typing = Employed for identifying the source of biological evidence Epigenomics = Potential solution for distinguishing identical twins using current forensic DNA STR typing methods</p> Signup and view all the answers

    Match the factor affecting DNA uniqueness with its description:

    <p>Polymorphisms in proteins, nucleic acids, and polysaccharides = Contribute to the uniqueness of individuals and their biological materials Low copy number DNA = Destructive nature limits its applicability in forensic investigations Identical twins' DNA sequences = Cannot be distinguished using current forensic DNA STR typing methods Different sequences of DNA in chromosomes = Allows for individualization based on DNA</p> Signup and view all the answers

    Match the following DNA analysis techniques with their applications:

    <p>Low Copy Number DNA analysis = Requires more stringent amplification conditions Forensic Genealogy = Searching for the DNA source by looking for a relative whose DNA profile is in the CODIS database Mitochondrial DNA (mtDNA) analysis = Used to assess the physical appearance of the DNA source Familial DNA testing = Used when the source DNA profile is NOT already in CODIS</p> Signup and view all the answers

    Match the following factors with their impact on DNA preservation:

    <p>Time = Can degrade DNA Temperature = Can degrade DNA Humidity (microorganisms) = Can degrade DNA Dry and frozen environment = Ideal for preservation</p> Signup and view all the answers

    Match the following DNA profiling terms with their meanings:

    <p>Alleles = Variant forms of a gene STR = Short Tandem Repeat, used in DNA profiling Phenotypes = Physical characteristics determined by genes Heteroplasmy = Presence of more than one type of organellar genome within a cell or individual</p> Signup and view all the answers

    Match the following scenarios with their implications in DNA analysis:

    <p>DNA False Positive = May lead to wrongful arrest or accusation DNA False Negative = May result in failure to identify the true source of DNA Advanced Parkinson’s disease = Can affect the accuracy of DNA testing Multiple offenders = Complicates interpretation of DNA evidence</p> Signup and view all the answers

    Match the following forensic case examples with their outcomes:

    <p>1999 UK case: man arrested based on 6 loci match to burglary scene sample = Highlighted issues of interpretation and false positive results in DNA testing UK Burglary Suspect with advanced Parkinson’s disease = Excluded and released after further loci testing performed at his lawyer’s request Cigarette butt tested from each scene in related burglaries = Odds of false match were calculated, considering the chance of a relative committing crime Identification of Tsarina and 3 daughters by mtDNA = Technical difficulties encountered in identifying Tsar</p> Signup and view all the answers

    Match the following statements with their implications in forensic DNA analysis:

    <p>Use of a condom = May complicate identification of DNA source Earlier/later consensual contact with another person = May lead to mixed stains, impacting accurate interpretation Innocence Project: Kerry Kotler = Advocates for careful interpretation of all areas of forensic evidence analysis DNA as an incredibly powerful tool but cannot supplant other evidential analysis = Emphasizes the need for careful interpretation in all areas of forensic evidence analysis</p> Signup and view all the answers

    Match the following biological evidence testing techniques with their descriptions:

    <p>Presumptive testing = Fast, sensitive tests, many of which can be done &quot;at the scene&quot; Confirmatory testing = Is the physiological fluid stain from a human. If not, then what species of animal is the source of the non-human bloodstain. Species testing = Specificity: unequivocal identification of the biological material. Frequently less sensitive and may be more time consuming. Protein typing = Traditional technique used for individualization based on DNA.</p> Signup and view all the answers

    Match the following concepts with their descriptions:

    <p>Biological Specificity = Screening tests to determine if a physiological fluid stain is from a human, and if not, to identify the species of animal. Individualization = The process of seeking the 'big picture' by considering the totality of the physical evidence to determine the source of unknown biological evidence. Familial DNA testing = Involves looking for a relative whose DNA profile is already in the CODIS database for forensic genealogy purposes. DNA typing = Current technique used for individualization based on DNA.</p> Signup and view all the answers

    Match the following types of DNA analysis with their significance in forensic investigations:

    <p>Mitochondrial DNA (mtDNA) analysis = Can help identify the source of biological materials at a crime scene, including physical appearance of the DNA source. Low copy number DNA analysis = Refers to analyzing minute amounts of DNA that may be present in forensic samples, allowing for identification in cases where DNA is scarce. Forensic DNA analysis = Approached with the 'big picture' in mind, considering the totality of the physical evidence to determine the source of unknown biological evidence. STR analysis = Detects alleles unique to individuals for forensic DNA profiling purposes.</p> Signup and view all the answers

    Match the following statements with their correct descriptions:

    <p>Non-scientist investigators' consideration of biological evidence tests = May occur without reference to its applicability to answering pertinent questions, emphasizing the need for scientists to ask the right questions during evidence analysis. Totality of physical evidence analysis = Emphasizes thinking beyond 'tests' applied to 'items' and considering what can be learned from all physical evidence to determine the source of unknown biological evidence. Contamination of biological samples = Is not completely avoidable by using gloves and proper packaging, highlighting the importance of proper collection and handling protocols for biological evidence. Biological material individualization based on DNA = Involves seeking beyond 'tests' and 'items' to determine who is the source of unknown biological evidence by considering all physical evidence.</p> Signup and view all the answers

    Study Notes

    Forensic Analysis of Physiological Fluids and DNA

    • Blood, semen, saliva, urine, and other biological fluids are commonly encountered in crimes of assault, homicide, and sexual assault.
    • Methods of analysis for blood are well developed for forensic purposes, while methods for other biological fluids vary in sophistication.
    • Morphological characteristics and DNA analysis, including nuclear and mitochondrial DNA, are used for unequivocal identification of physiological fluids.
    • Polymorphisms in proteins, nucleic acids, and polysaccharides contribute to the uniqueness of individuals and their biological materials.
    • Secretor status, controlled genetically, affects the presence of ABO agglutinogens in body fluids, with around 80% of the population classified as secretors.
    • Secretor status is correlated with another cellular antigen system known as the Lewis system, impacting the analysis of mixed stains in sexual assault cases.
    • DNA analysis can link a suspect to a crime scene or victim, with sensitivity, specificity, and controls being crucial for accurate interpretation.
    • Common sources of DNA at crime scenes include blood, semen, saliva, urine, hair, teeth, bone, and tissue, as well as pattern evidence like fingerprints.
    • Biological evidence can easily be contaminated or destroyed, emphasizing the importance of proper collection and handling protocols.
    • Forensic DNA analysis seeks to identify the source of biological evidence and may employ techniques such as electrophoresis, ELISA, and DNA/RNA typing.
    • Identical twins cannot be distinguished using current forensic DNA STR typing methods, but epigenomics may offer a potential solution for differentiation.
    • Every human being is biologically unique due to the different sequences of DNA in their chromosomes, allowing for individualization based on DNA.

    Forensic Analysis of Physiological Fluids and DNA

    • Blood, semen, saliva, urine, and other biological fluids are commonly encountered in crimes of assault, homicide, and sexual assault.
    • Methods of analysis for blood are well developed for forensic purposes, while methods for other biological fluids vary in sophistication.
    • Morphological characteristics and DNA analysis, including nuclear and mitochondrial DNA, are used for unequivocal identification of physiological fluids.
    • Polymorphisms in proteins, nucleic acids, and polysaccharides contribute to the uniqueness of individuals and their biological materials.
    • Secretor status, controlled genetically, affects the presence of ABO agglutinogens in body fluids, with around 80% of the population classified as secretors.
    • Secretor status is correlated with another cellular antigen system known as the Lewis system, impacting the analysis of mixed stains in sexual assault cases.
    • DNA analysis can link a suspect to a crime scene or victim, with sensitivity, specificity, and controls being crucial for accurate interpretation.
    • Common sources of DNA at crime scenes include blood, semen, saliva, urine, hair, teeth, bone, and tissue, as well as pattern evidence like fingerprints.
    • Biological evidence can easily be contaminated or destroyed, emphasizing the importance of proper collection and handling protocols.
    • Forensic DNA analysis seeks to identify the source of biological evidence and may employ techniques such as electrophoresis, ELISA, and DNA/RNA typing.
    • Identical twins cannot be distinguished using current forensic DNA STR typing methods, but epigenomics may offer a potential solution for differentiation.
    • Every human being is biologically unique due to the different sequences of DNA in their chromosomes, allowing for individualization based on DNA.

    Forensic Analysis of Physiological Fluids and DNA

    • Blood, semen, saliva, urine, and other biological fluids are commonly encountered in crimes of assault, homicide, and sexual assault.
    • Methods of analysis for blood are well developed for forensic purposes, while methods for other biological fluids vary in sophistication.
    • Morphological characteristics and DNA analysis, including nuclear and mitochondrial DNA, are used for unequivocal identification of physiological fluids.
    • Polymorphisms in proteins, nucleic acids, and polysaccharides contribute to the uniqueness of individuals and their biological materials.
    • Secretor status, controlled genetically, affects the presence of ABO agglutinogens in body fluids, with around 80% of the population classified as secretors.
    • Secretor status is correlated with another cellular antigen system known as the Lewis system, impacting the analysis of mixed stains in sexual assault cases.
    • DNA analysis can link a suspect to a crime scene or victim, with sensitivity, specificity, and controls being crucial for accurate interpretation.
    • Common sources of DNA at crime scenes include blood, semen, saliva, urine, hair, teeth, bone, and tissue, as well as pattern evidence like fingerprints.
    • Biological evidence can easily be contaminated or destroyed, emphasizing the importance of proper collection and handling protocols.
    • Forensic DNA analysis seeks to identify the source of biological evidence and may employ techniques such as electrophoresis, ELISA, and DNA/RNA typing.
    • Identical twins cannot be distinguished using current forensic DNA STR typing methods, but epigenomics may offer a potential solution for differentiation.
    • Every human being is biologically unique due to the different sequences of DNA in their chromosomes, allowing for individualization based on DNA.

    Forensic Analysis of Physiological Fluids and DNA

    • Blood, semen, saliva, urine, and other biological fluids are commonly encountered in crimes of assault, homicide, and sexual assault.
    • Methods of analysis for blood are well developed for forensic purposes, while methods for other biological fluids vary in sophistication.
    • Morphological characteristics and DNA analysis, including nuclear and mitochondrial DNA, are used for unequivocal identification of physiological fluids.
    • Polymorphisms in proteins, nucleic acids, and polysaccharides contribute to the uniqueness of individuals and their biological materials.
    • Secretor status, controlled genetically, affects the presence of ABO agglutinogens in body fluids, with around 80% of the population classified as secretors.
    • Secretor status is correlated with another cellular antigen system known as the Lewis system, impacting the analysis of mixed stains in sexual assault cases.
    • DNA analysis can link a suspect to a crime scene or victim, with sensitivity, specificity, and controls being crucial for accurate interpretation.
    • Common sources of DNA at crime scenes include blood, semen, saliva, urine, hair, teeth, bone, and tissue, as well as pattern evidence like fingerprints.
    • Biological evidence can easily be contaminated or destroyed, emphasizing the importance of proper collection and handling protocols.
    • Forensic DNA analysis seeks to identify the source of biological evidence and may employ techniques such as electrophoresis, ELISA, and DNA/RNA typing.
    • Identical twins cannot be distinguished using current forensic DNA STR typing methods, but epigenomics may offer a potential solution for differentiation.
    • Every human being is biologically unique due to the different sequences of DNA in their chromosomes, allowing for individualization based on DNA.

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