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Questions and Answers
What is the primary source of illumination for a compound microscope?
What is the primary source of illumination for a compound microscope?
- Visible light (correct)
- Ultraviolet light
- Laser light
- Infrared light
How much can a compound microscope typically magnify an object?
How much can a compound microscope typically magnify an object?
- Approximately 1000 times (correct)
- Up to 100 times
- Up to 500 times
- Over 2000 times
Which component of a compound microscope contains the ocular lens?
Which component of a compound microscope contains the ocular lens?
- The objective lens
- The eyepiece (correct)
- The condenser
- The stage
What is a significant advancement of microscopes since their initial discovery?
What is a significant advancement of microscopes since their initial discovery?
Which type of microscope uses more than one magnifying lens?
Which type of microscope uses more than one magnifying lens?
What distinguishes algae from plants in terms of taxonomic classification?
What distinguishes algae from plants in terms of taxonomic classification?
Which of the following is a characteristic feature that differentiates plants from algae?
Which of the following is a characteristic feature that differentiates plants from algae?
What type of reproduction is commonly associated with plants?
What type of reproduction is commonly associated with plants?
Which of the following types of algae is known for causing red tide?
Which of the following types of algae is known for causing red tide?
What is the primary component of diatom cell walls?
What is the primary component of diatom cell walls?
Which statement correctly describes prokaryotic cells?
Which statement correctly describes prokaryotic cells?
What energy storage form is common to both algae and plants?
What energy storage form is common to both algae and plants?
What habitat is primarily associated with plants compared to algae?
What habitat is primarily associated with plants compared to algae?
Which type of bacteria has a tuft of flagella on one end?
Which type of bacteria has a tuft of flagella on one end?
What structure do bacteria use to exchange genetic material?
What structure do bacteria use to exchange genetic material?
Which component provides rigid support and shape to bacteria?
Which component provides rigid support and shape to bacteria?
What component makes the bacterial endospore resistant to heat and drying?
What component makes the bacterial endospore resistant to heat and drying?
Which structure can be stained with crystal violet in acid-fast bacteria?
Which structure can be stained with crystal violet in acid-fast bacteria?
Which among the following cell wall components is unique to gram positive bacteria?
Which among the following cell wall components is unique to gram positive bacteria?
What is the primary function of a bacterial capsule?
What is the primary function of a bacterial capsule?
Which layer of acid-fast bacteria is hydrophobic due to lipid presence?
Which layer of acid-fast bacteria is hydrophobic due to lipid presence?
Which areas of the skin are characterized by higher moisture levels, body temperature, and surface lipids?
Which areas of the skin are characterized by higher moisture levels, body temperature, and surface lipids?
What type of microorganisms predominantly inhabit the moist regions of the skin?
What type of microorganisms predominantly inhabit the moist regions of the skin?
Which organism is the major resident aerobic flora on the skin?
Which organism is the major resident aerobic flora on the skin?
Which of the following is a characteristic of dry sites of skin like hands and forearms?
Which of the following is a characteristic of dry sites of skin like hands and forearms?
What type of microorganisms are primarily present in the deeper structures of the skin?
What type of microorganisms are primarily present in the deeper structures of the skin?
What percentage of the dry weight of a bacterial cell is nitrogen?
What percentage of the dry weight of a bacterial cell is nitrogen?
Which ion is explicitly mentioned as stabilizing ribosomes and cell membranes?
Which ion is explicitly mentioned as stabilizing ribosomes and cell membranes?
What term describes bacteria that cannot survive in the presence of oxygen?
What term describes bacteria that cannot survive in the presence of oxygen?
What role does calcium play in bacterial cells?
What role does calcium play in bacterial cells?
What are growth factors necessary for in bacterial cells?
What are growth factors necessary for in bacterial cells?
Which type of bacteria can grow under both aerobic and anaerobic conditions?
Which type of bacteria can grow under both aerobic and anaerobic conditions?
What type of bacteria requires the addition of carbon dioxide to enhance growth?
What type of bacteria requires the addition of carbon dioxide to enhance growth?
Which of the following is necessary for the maintenance of protein structure in bacteria?
Which of the following is necessary for the maintenance of protein structure in bacteria?
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Study Notes
Microbiology Overview
- Experiments and vaccine discoveries in the 1940s and 1950s have advanced prevention and control of fatal infectious diseases.
Microscopy
- Microorganisms are too small to be seen without a microscope.
- Microscopes can magnify organisms 100 to 1,000 times their original size.
- The microscope was first discovered in the 1600s and has evolved significantly since.
Types of Microscopes
- Compound Microscope: Features multiple lenses; magnifies objects up to 1,000 times.
- Main illumination source is visible light.
- Comprises an ocular lens (10x magnification) and objective lens positioned above the specimen.
Comparison of Algae and Plants
- Taxonomy: Algae belong to Kingdom Protista; plants to Kingdom Plantae.
- Cellular Structure: Algae can be unicellular or multicellular; plants are multicellular.
- Photosynthesis: Both groups photosynthesize using carbon dioxide.
- Storage: Both store energy as starch.
- Vascular System: Absent in algae, present in plants, facilitating nutrient dispersion.
- Habitat: Algae mostly in water; plants typically rooted in soil.
- Reproduction: Algae reproduce both asexually and sexually; plants reproduce sexually (complex).
Diatoms and Dinoflagellates
- Diatoms: Unicellular algae found in freshwater and saltwater; cell walls contain silica, useful for filtration and abrasives.
- Dinoflagellates: Contribute to atmospheric oxygen and are key in the food chain; responsible for "red tide," producing neurotoxins that cause paralytic shellfish poisoning.
Cell Classification
- Cells categorized as Prokaryotic (bacteria) or Eukaryotic.
- Prokaryotic cells are usually unicellular, lack a true nucleus, and reproduce via binary fission.
Bacterial Structures
- Flagella types:
- Lophotrichous: tuft at one end
- Monotrichous: single at one end
- Peritrichous: flagella all around
- Endospore: Resistant to heat and drying due to dipicolinic acid.
- Cell Wall: Provides rigid support and shape.
Essential Elements for Bacteria
- Nitrogen (14% dry weight) and phosphorus (4%) are vital for nucleic acid and ATP synthesis.
- Inorganic ions like magnesium, potassium, calcium, and iron support various cellular functions.
Growth Factors
- Essential for bacterial growth include vitamin B complex and amino acids.
Physical Requirements for Bacterial Growth
- Moisture/Water: Critical for nutrient acquisition.
- Oxygen: Aerobic microbes require oxygen; classified as aerobes or anaerobes.
- Strict aerobes need oxygen; obligate anaerobes cannot survive in its presence, while facultative organisms can thrive in both environments.
- Some bacteria, termed microaerophiles, grow optimally at low oxygen levels.
Human Skin Microbiome
- Skin regions vary in moisture levels and microbial diversity.
- Higher moisture areas (e.g., axilla) inhabited mainly by gram-negative bacilli.
- Dry areas host Staphylococcus epidermidis and Staphylococcus hominis predominantly.
Normal Flora of the Skin
- Staphylococcus epidermidis: Major skin resident (90% of aerobic flora).
- Staphylococcus aureus: Commonly found in the nose and perineum; count varies with age.
- Micrococci (e.g., Micrococcus luteus): Comprise a significant portion of skin micrococci.
- Diphtheroids (Coryneforms): Lipophilic forms common in moist skin areas like the axilla.
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