BIOL 2260 Lesson 06: Microscopy, Staining, and Classification
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Questions and Answers

Which type of electromagnetic radiation has the widest wavelength?

  • Gamma rays
  • Radio waves (correct)
  • X-rays
  • UV light

    • What determines the ability to distinguish two separate points as distinct in microscopy?

  • Contrast
  • Wavelength of radiation
  • Resolution (correct)
  • Magnification

    • In light refraction, what happens when light goes through a less dense medium and then refracts in a more dense medium like glass?

  • It becomes stronger (correct)
  • It becomes inverted
  • It becomes reversed
  • It becomes weaker

    • What type of lens does a microscope have?

    <p>Convex lens</p> Signup and view all the answers

    Which type of electromagnetic radiation has the shortest wavelength?

    <p>X-rays</p> Signup and view all the answers

    What is the relevance of contrast in microscopy?

    <p>It ensures that the specimen standouts</p> Signup and view all the answers

    What is the main difference between resolution and contrast in microscopy?

    <p>Resolution refers to the ability to distinguish close points, while contrast refers to the difference in intensity between objects.</p> Signup and view all the answers

    Which type of microscope is best used for pale/translucent specimens?

    <p>Dark-field microscope</p> Signup and view all the answers

    What is the function of confocal microscopes?

    <p>To construct 3-D images from individual digitized images using fluorescent dyes and a UV laser.</p> Signup and view all the answers

    What is the typical magnification range of electron microscopes?

    <p>100x to 100,000x</p> Signup and view all the answers

    Which type of microscope uses UV light onto the specimen and allows it to radiate light back to the microscope?

    <p>Fluorescence microscope</p> Signup and view all the answers

    What is the main advantage of dark-field microscopes?

    <p>They increase the contrast and can see finer details like cell structures.</p> Signup and view all the answers

    What type of microscope enhances contrast by using interference between two light beams and creates a 3-D shadowed appearance of the specimen?

    <p>Differential interference contrast microscope</p> Signup and view all the answers

    How does bright-field microscopy differ from compound microscopy?

    <p>Bright-field microscopy contains a single magnifying lens, while compound microscopy has one or two ocular lenses.</p> Signup and view all the answers

    What type of microscopy is best used for examining living organisms that would be damaged or altered with staining?

    <p>Phase-contrast microscopy</p> Signup and view all the answers

    What is the main advantage of electron microscopes over light microscopes?

    <p>They have higher resolution and can distinguish finer details.</p> Signup and view all the answers

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