BIOL 2260 Lesson 06: Microscopy, Staining, and Classification

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Which type of electromagnetic radiation has the widest wavelength?

Gamma rays

Radio waves (correct)

X-rays

UV light

What determines the ability to distinguish two separate points as distinct in microscopy?

Contrast

Wavelength of radiation

Resolution (correct)

Magnification

In light refraction, what happens when light goes through a less dense medium and then refracts in a more dense medium like glass?

It becomes stronger (correct)

It becomes inverted

It becomes reversed

It becomes weaker

What type of lens does a microscope have?

Convex lens Signup and view all the answers

Which type of electromagnetic radiation has the shortest wavelength?

X-rays Signup and view all the answers

What is the relevance of contrast in microscopy?

It ensures that the specimen standouts Signup and view all the answers

What is the main difference between resolution and contrast in microscopy?

Resolution refers to the ability to distinguish close points, while contrast refers to the difference in intensity between objects. Signup and view all the answers

Which type of microscope is best used for pale/translucent specimens?

Dark-field microscope Signup and view all the answers

What is the function of confocal microscopes?

To construct 3-D images from individual digitized images using fluorescent dyes and a UV laser. Signup and view all the answers

What is the typical magnification range of electron microscopes?

100x to 100,000x Signup and view all the answers

Which type of microscope uses UV light onto the specimen and allows it to radiate light back to the microscope?

Fluorescence microscope Signup and view all the answers

What is the main advantage of dark-field microscopes?

They increase the contrast and can see finer details like cell structures. Signup and view all the answers

What type of microscope enhances contrast by using interference between two light beams and creates a 3-D shadowed appearance of the specimen?

Differential interference contrast microscope Signup and view all the answers

How does bright-field microscopy differ from compound microscopy?

Bright-field microscopy contains a single magnifying lens, while compound microscopy has one or two ocular lenses. Signup and view all the answers

What type of microscopy is best used for examining living organisms that would be damaged or altered with staining?

Phase-contrast microscopy Signup and view all the answers

What is the main advantage of electron microscopes over light microscopes?

They have higher resolution and can distinguish finer details. Signup and view all the answers

BIOL 2260 Lesson 06: Microscopy, Staining, and Classification

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