BIOL 203: Intro to Microbiology
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Questions and Answers

Which of the following contributions is NOT directly associated with the early development of microbiology as described?

  • Synthesizing antibiotics to combat bacterial infections. (correct)
  • Establishing steam sterilization methods for growth media.
  • Pioneering the use of Petri dishes for culturing microorganisms.
  • Developing techniques to estimate the number of bacteria in solution through colony formation.

Koch's postulates require that the suspected causative agent be present in healthy hosts but absent from diseased ones.

False (B)

What is the primary purpose of the Gram stain technique developed by Hans Christian Gram?

To differentiate bacteria into Gram-positive and Gram-negative groups.

Ignaz Semmelweis significantly reduced healthcare-associated infections by promoting what simple practice? __________

<p>handwashing</p> Signup and view all the answers

Joseph Lister is best known for his contributions to:

<p>The advancement of antiseptic surgery using carbolic acid. (A)</p> Signup and view all the answers

Match each scientist with their contribution to microbiology:

<p>Robert Koch = Developed postulates to prove the cause of infectious diseases Hans Christian Gram = Developed a staining technique to differentiate bacteria Ignaz Semmelweis = Promoted handwashing to reduce healthcare-associated infections Joseph Lister = Advanced antiseptic surgery using carbolic acid</p> Signup and view all the answers

Which step of Koch's postulates involves isolating the disease-causing agent?

<p>Growing the agent outside of the host. (B)</p> Signup and view all the answers

Koch's postulates can be universally applied to prove the cause of every infectious disease.

<p>False (B)</p> Signup and view all the answers

Which of the following best describes Antoni van Leeuwenhoek's primary contribution to microbiology?

<p>Creating high-quality microscopes that revealed the microbial world (D)</p> Signup and view all the answers

Protozoa, algae, and nonmicrobial parasitic worms are studied in microbiology primarily due to their genetic similarity to bacteria.

<p>False (B)</p> Signup and view all the answers

What is the fundamental difference between prokaryotic and eukaryotic organisms at the cellular level?

<p>Prokaryotic cells lack a nucleus and other membrane-bound organelles, which are present in eukaryotic cells.</p> Signup and view all the answers

The belief that life could arise spontaneously from non-living matter was known as the theory of ______ generation.

<p>spontaneous</p> Signup and view all the answers

Match the scientist with their contribution to the spontaneous generation debate:

<p>Redi = Challenged spontaneous generation with experiments involving meat and flies. Needham = Supported spontaneous generation with experiments involving heated broth. Spallanzani = Challenged spontaneous generation with experiments involving sealed flasks of broth. Pasteur = Disproved spontaneous generation with swan-necked flask experiments.</p> Signup and view all the answers

What was the key outcome of Pasteur's fermentation experiments that greatly impacted the world?

<p>The understanding that microorganisms cause fermentation, leading to food preservation techniques (D)</p> Signup and view all the answers

Koch's postulates are a set of criteria used to establish a causative link between a specific microorganism and all diseases.

<p>False (B)</p> Signup and view all the answers

What is the significance of the Gram stain in microbiology?

<p>The Gram stain is a differential staining technique used to classify bacteria based on their cell wall structure, aiding in identification and treatment decisions.</p> Signup and view all the answers

Which of the following best describes Eduard Buchner's contribution to science?

<p>He demonstrated that fermentation is performed by enzymes, not requiring living cells. (D)</p> Signup and view all the answers

The germ theory of disease explains all types of diseases, including genetic disorders and chronic conditions.

<p>False (B)</p> Signup and view all the answers

What is the term for the study of the causes of disease, a field in which Robert Koch significantly contributed?

<p>etiology</p> Signup and view all the answers

Robert Koch identified _ _ _ _ _ _ _ _ _ as the causative agent of tuberculosis.

<p>Mycobacterium tuberculosis</p> Signup and view all the answers

Before Pasteur's work, what were some common explanations for the cause of diseases?

<p>Evil spirits, astrological signs, imbalances in bodily fluids, and foul vapors (C)</p> Signup and view all the answers

Which of the following is a direct contribution of Robert Koch to the advancement of microbiology?

<p>Development of staining techniques for bacterial cells (D)</p> Signup and view all the answers

Industrial microbiology is a field that studies the use of microorganisms to produce desired products on a large scale.

<p>True (A)</p> Signup and view all the answers

Match the scientist with their contribution:

<p>Eduard Buchner = Demonstrated fermentation by enzymes Robert Koch = Identified the cause of anthrax Louis Pasteur = Developed the germ theory of disease</p> Signup and view all the answers

Which of the following fields directly applies biochemical knowledge to improve agriculture?

<p>Design of herbicides and pesticides (B)</p> Signup and view all the answers

Molecular biology focuses primarily on understanding cellular functions at a macroscopic level.

<p>False (B)</p> Signup and view all the answers

What is the primary goal of gene therapy?

<p>inserting, removing, or modifying genes</p> Signup and view all the answers

The use of living organisms to detoxify polluted environments is known as ______.

<p>bioremediation</p> Signup and view all the answers

Match the following disciplines with their focuses:

<p>Biochemistry = Study of metabolism Genetics = Study of inheritance Environmental microbiology = Study of microorganisms in various habitats Immunology = Study of the body's defense against pathogens</p> Signup and view all the answers

Which process relies on the ability of microorganisms to convert substances into different chemical forms?

<p>Chemical cycling (C)</p> Signup and view all the answers

Serology is the study of the body's defense against specific pathogens.

<p>False (B)</p> Signup and view all the answers

If a researcher is manipulating genes for applications such as creating insulin from bacteria, which field are they working in?

<p>Recombinant DNA technology (D)</p> Signup and view all the answers

Which of the following characteristics distinguishes Archaea from Bacteria?

<p>Archaea are more closely related to eukaryotes, and some can tolerate extreme environments. (A)</p> Signup and view all the answers

All bacteria are pathogenic to humans.

<p>False (B)</p> Signup and view all the answers

What is the primary difference between molds and yeasts within the Fungi domain?

<p>Molds are multicellular filaments, while yeasts are unicellular and round.</p> Signup and view all the answers

The cell walls of fungi are composed of either chitin or ________.

<p>glucomannan</p> Signup and view all the answers

Match the following microorganisms with their domain:

<p>Bacteria = Bacteria Archaea = Archaea Fungi = Eukarya Algae = Eukarya</p> Signup and view all the answers

Which of the following eukaryotic microorganisms is characterized by its ability to perform photosynthesis?

<p>Algae (B)</p> Signup and view all the answers

Protozoa possess cell walls, similar to plants and bacteria.

<p>False (B)</p> Signup and view all the answers

What is the primary mode of reproduction for Bacteria and Archaea?

<p>asexual</p> Signup and view all the answers

The cell walls of most Bacteria contain a unique substance called ________.

<p>peptidoglycan</p> Signup and view all the answers

Which of the following microorganisms is typically multicellular and reproduces sexually, but lays microscopic eggs?

<p>Parasitic Worms (D)</p> Signup and view all the answers

What was Florence Nightingale's primary contribution to the field of medicine?

<p>Introducing cleanliness and antiseptic techniques into nursing practice. (A)</p> Signup and view all the answers

John Snow's work mapping cholera cases in London supported the idea that unsanitary conditions were a major factor in disease spread.

<p>True (A)</p> Signup and view all the answers

How did Edward Jenner's experiment contribute to the development of immunology?

<p>He infected a boy with cowpox, providing immunity to smallpox, which led to vaccination.</p> Signup and view all the answers

Paul Ehrlich sought a chemical "magic bullet" to selectively destroy ______.

<p>pathogens</p> Signup and view all the answers

Which of the following best describes the focus of epidemiology, as established by John Snow's work?

<p>Examining the occurrence, distribution, and spread of disease. (B)</p> Signup and view all the answers

Match the following scientists with their main contribution to microbiology:

<p>Florence Nightingale = Introduced cleanliness and antiseptic techniques in nursing John Snow = Mapped cholera cases and linked it to water supply Edward Jenner = Developed vaccination against smallpox Paul Ehrlich = Pioneered chemotherapy with the “magic bullet” concept</p> Signup and view all the answers

Which question reflects a major focus of modern microbiological research regarding disease?

<p>How can we defend against disease? (B)</p> Signup and view all the answers

The study of the body’s defenses against pathogens is called chemotherapy.

<p>False (B)</p> Signup and view all the answers

Flashcards

Koch's Postulates

Steps to prove the cause of an infectious disease.

Gram Stain

A technique to classify bacteria as Gram positive or negative.

Ignaz Semmelweis

Promoted handwashing to reduce healthcare-associated infections.

Joseph Lister

Pioneered antiseptic surgery using carbolic acid.

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Bacteria in diseased tissue

First photomicrograph showing bacteria's role in disease.

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Steam Sterilization

Method for sterilizing growth media using steam.

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Petri Dishes

Containers used to culture bacteria on solid media.

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Lab techniques for transferring bacteria

Methods used to move bacteria between different media.

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Biochemistry

The study of metabolism and its practical applications.

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Molecular Biology

Explains how the cell functions at the molecular level.

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Recombinant DNA Technology

Manipulating genes for practical applications.

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Gene Therapy

Inserting, removing, or modifying genes in human cells.

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Environmental Microbiology

Study of microorganisms in habitats like soil and water.

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Bioremediation

Use of living organisms to detoxify polluted environments.

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Serology

Study of blood serums that fight disease.

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Immunology

Study of the body’s defense against specific pathogens.

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Florence Nightingale

Pioneered cleanliness and antiseptic techniques in nursing.

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Statistical Comparisons

Used by Nightingale to link poor conditions to high mortality rates.

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John Snow

Mapped cholera cases to show their link to a water supply.

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Infection Control

Study and strategies to prevent disease spread, founded by Snow's work.

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Edward Jenner

Developed the first vaccine using cowpox to immunize against smallpox.

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Paul Ehrlich

Sought a 'magic bullet' for targeting pathogens without harming humans.

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Modern Microbiology Questions

Focuses on chemical reactions of life, gene function, microorganisms' roles, and disease defense.

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Leeuwenhoek's Contribution

He created microscopes that revealed the microbial world.

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Microbes

Organisms too small to be seen without a microscope.

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Prokaryotic vs Eukaryotic

Prokaryotic organisms lack a nucleus; eukaryotic have one.

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Spontaneous Generation

The belief that living organisms can arise from non-living matter.

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Pasteur's Fermentation Experiment

Demonstrated microorganisms' role in fermentation, impacting food industries.

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Gram Staining

A technique developed by Hans Christian Gram to differentiate bacteria.

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Immunology Pioneers

Scientists who contributed to the development of vaccines.

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Industrial Microbiology

The study and application of microorganisms in industry.

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Eduard Buchner

A scientist who showed that fermentation is due to enzymes, not living cells.

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Germ Theory of Disease

The theory that pathogens cause infectious diseases.

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Robert Koch

A key figure in identifying disease-causing bacteria.

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Bacillus anthracis

The bacterium that causes anthrax.

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Mycobacterium tuberculosis

The bacterium that causes tuberculosis.

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Etiology

The study of the causes of diseases.

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Staining Techniques

Methods used to color bacterial cells for easier observation.

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Bacteria

Unicellular organisms with peptidoglycan cell wall, mainly asexual reproduction.

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Archaea

Unicellular organisms similar to eukaryotes, with polysaccharide or protein cell walls, asexual reproduction.

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Fungi

Eukaryotic organisms with cell walls of chitin or glucomannan, can be unicellular or multicellular, reproduce asexually or sexually.

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Protozoa

Unicellular organisms without cell walls, can reproduce both asexually and sexually, capable of locomotion.

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Algae

Photosynthetic eukaryotic organisms, unicellular or multicellular, with cellulose cell walls.

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Small multicellular animals

Multicellular organisms without cell walls, usually reproduce sexually.

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Peptidoglycan

A polymer that makes up the cell wall of most bacteria.

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Chitin

A fibrous substance consisting of polysaccharides, found in the cell walls of fungi.

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Locomotion in Protozoa

The ability of protozoa to move independently.

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Extreme environments in Archaea

Conditions where some archaea thrive, such as high heat or salinity.

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Study Notes

Course Information

  • Course name: BIOL 203 – INTRODUCTION TO MICROBIOLOGY
  • Instructor: Dr. Eugene Chu
  • Email: [email protected]
  • Office: FIR 486

Course Description

  • Introduces basic microbiology principles with applications to human health.
  • Focuses primarily on bacterial biology.
  • Includes laboratory exercises covering standard microbiological lab techniques.

About the Textbook

  • There is a textbook for lectures and a separate lab manual.
  • Textbook title: Microbiology with Diseases by Body System; Fifth Edition
  • Author: Robert W. Bauman
  • Lab manual title: Microbiology, Laboratory Theory & Application, Brief; Third Edition
  • Authors: Michael J. Leboffe and Burton E. Pierce.

About Moodle

  • All course materials and communications are online via Moodle (eLearn).

Email Communication

  • Instructor is available via email ([email protected]).
  • Moodle messenger is not used.
  • Allow 24 business hours for email responses.
  • Avoid emailing the night before the exam.
  • Avoid checking emails on weekend.

Course Format

  • Two 80-minute lectures per week.
  • One 3-hour lab session per week.
  • Schedules are posted on Moodle, updating if needed.
  • Check the most current schedule before asking about exam dates, topics, or readings.

Course Evaluation

  • Midterms: Two midterms, each worth 15% (30% total).
  • Final Exam: One final exam, worth 30%.
  • Assignments: Assignments worth 10%.
  • Labs: Labs worth 30%.
  • Passing the course requires a minimum of 50% on both the lecture and lab portions.

Midterm Exams

  • Taken in class during the lecture period (~75 minutes).
  • Two exams, each worth 15%.

Final Exam

  • Taken at the end of the year.
  • In-person exam covering material after midterm 2.
  • Worth 30% of total grade.
  • 170 minutes long.

Assignments

  • Short assignments are given in class and on eLearn.
  • Assignments are available for each chapter.
  • Students design two questions for each chapter assignment.

Question Design Assignments

  • Two questions per chapter are needed.
  • Marking criteria: Completion, correctness, and creativity.
  • Higher-level questions (e.g., applying) earn more bonus marks.
  • Questions are submitted on eLearn.

Labs

  • Labs are worth 30%.
  • Evaluation details:
    • Lab exam 1 (10%)
    • Lab exam 2 (10%)
    • Unknown Organism Project (8%)
    • Professionalism (2%)
    • Pre-lab quizzes with 5 questions each.

Grading Profile

  • Letter grades based on a range format (e.g., A+ = 90-100%).
  • Rounding but no "bumping up" grades.
  • 50% required to pass.
  • No redos for exams.

Classroom Etiquette (Students)

  • Arrive on time.
  • Be respectful of others.
  • Turn off cell phones.
  • Be prepared for class.

Classroom Etiquette (Instructor)

  • Arrive on time.
  • Be respectful of students.
  • Turn off cell phones.
  • Be prepared for class.

Missed Classes

  • Responsibility to obtain missed information from a classmate in case of absence.

Missed Exams

  • In general, a score of 0 for missed exams.
  • Exceptions may be allowed with:
    • Circumstances beyond the student's control.
    • Prior notification to the instructor (if possible, or by the time of the exam).
    • Supporting documentation submitted within 5 days of the missed exam.
    • Full participation in the course until the circumstances arose.

Accommodations

  • Contact Accessibility Services for accommodation requests.
  • Location and hours: Birch building, Room 284, Monday to Friday 8:30am to 4:00pm.
  • Phone: 604.983.7526
  • Email: [email protected]

Academic Dishonesty

  • Cheating on tests results in a failing grade (F) and possible expulsion.
  • Plagiarism of any course assignment or lab report is considered cheating.

Tips for Success

  • Unanswered questions in the lecture slides are marked in Red.
  • New information is flagged with a "new" icon in the top right corner.

Learning Outcomes (Chapters 1-3)

  • A series of learning outcomes covering various aspects of microbiology, including historical context, defining microbes, comparing different types of microbes, and the history behind microbiology principles.

Learning Outcomes (Chapters 4-5)

  • Comprehensive list of learning outcomes covering different crucial aspects of Microbiology, including the various causes of disease, how we can mitigate the risk of infections, and the development and impact of microbiology principles.

Modern Age of Microbiology

  • Major questions driving current microbiology research:
    • Basic chemical reactions of life
    • How genes work
    • Microorganisms' role in the environment
    • How we defend against disease

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Description

Introduction to Microbiology (BIOL 203) course overview. Includes textbook details, lab manual information, and communication guidelines with Dr. Eugene Chu, the instructor. All materials and communications are available via Moodle.

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