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Questions and Answers
What are the three main domains of life, and provide a brief description of each?
What are the three main domains of life, and provide a brief description of each?
The three main domains of life are Eubacteria, Archaebacteria, and Eukarya. Eubacteria are the most well-studied bacteria found in various environments. Archaebacteria are less characterized and inhabit extreme environments. Eukarya evolved from the same branch as Archaea and are distinguished by their nucleus and various membrane-bound organelles.
What are the distinguishing characteristics of eukaryotes?
What are the distinguishing characteristics of eukaryotes?
Eukaryotes are distinguished by the presence of a nucleus and various membrane-bound organelles, including mitochondria, endoplasmic reticulum, Golgi complexes, and lysosomes.
Describe the composition of the cytoplasm in Escherichia coli.
Describe the composition of the cytoplasm in Escherichia coli.
The cytoplasm of Escherichia coli contains numerous components, including ribosomes, enzymes, metabolites, cofactors, and inorganic ions.
What is the role of cytoskeletal components in cells, and what are their characteristics?
What is the role of cytoskeletal components in cells, and what are their characteristics?
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Explain the significance of carbon in the chemistry of living organisms.
Explain the significance of carbon in the chemistry of living organisms.
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What are the four main categories of organic compounds essential for cellular materials, and provide an example for each?
What are the four main categories of organic compounds essential for cellular materials, and provide an example for each?
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What is a chiral center, and how is it related to geometric isomers?
What is a chiral center, and how is it related to geometric isomers?
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What is the RS system, and when is it used?
What is the RS system, and when is it used?
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Study Notes
Course Information
- Course title: Biochemistry
- Course code: CHY1003
- Course type: LTP
- Module No. 1
- Instructor: Dr. N. Vignesh
- Employee ID: 100589
- Department: School of Biosciences Engineering and Technology
- Semester: Winter 2025-26
Course Objectives
- Learn basic principles of structural and functional properties of biomolecules in living organisms
- Understand chemical phenomena in biological systems
- Analyze diseases and disorders involving biomolecules
Course Outcomes
- Understand the structure, properties, and functions of biomolecules (amino acids, proteins, carbohydrates, and nucleic acids)
- Understand the chemical basis of biological phenomena and basic metabolic pathways
- Analyze physiological conditions influencing biomolecule structures and functions
- Appreciate the importance of biological chemistry in healthcare and medicine
- Apply biochemistry concepts in biomedical or biochemical engineering fields
Textbooks and References
- Principles of Biochemistry by Lehninger, A, Butterworth Publishers, New York
- Biochemistry by Stryer L, W.H.Freeman and Company
- Harpers Illustrated Biochemistry, McGraw-Hill Education
- BIOCHEMICAL CALCULATIONS. How to Solve Mathematical Problems in General Biochemistry, Irwin H. Segel, Wiley New York
- Biochemistry by Voet &Voet, Wiley New York
- Biochemistry by Nelson and Cox, Palgrave Macmilan, Freeman Edn.
- Fundamentals of Biochemistry by Dr.J.L.Jain, Sunjay Jain and Nitin Jain, S.Chand Publishers
Origin of the Universe and Life
- 15-20 billion years ago, the universe arose from a hot, energy-rich eruption of subatomic particles
- Hydrogen and helium formed initially
- Material condensed due to gravity, forming stars
- Supernovae released energy to fuse simpler elements into more complex ones
- After billions of years, Earth and its elements formed
Origin of Life
- 4 billion years ago, life emerged on Earth
- Simple microorganisms extracted energy from organic compounds or sunlight
- These organisms created complex biomolecules from simple elements/compounds
Origin of Life Timeline
- Oceans formed
- Prebiotic Epoch
- Pre-RNA World
- RNA World
- DNA/Protein World
- Diversification for Eukaryotes occurred (Plants, Animals, Fungi)
- First evidence of life was found 3.5 Billion years ago
Distinguishing Features of Biotic Phase
- High degree of chemical complexity and microscopic organization
- Systems for extracting, transforming, and using energy from the environment
- Capacity for precise self-replication and self-assembly
- Mechanisms for sensing and responding to changes in their surroundings
- Defined functions for components and regulated interactions
- History of evolutionary change
Cell Structure and Function
- Biochemistry describes molecular structures, mechanisms, and chemical processes common to all living organisms
- Unity and diversity of organisms apparent even at the cellular level
- Single-celled organisms are microscopic; multicellular organisms contain various cell types with varying sizes, shapes, and functions
Cell Size Limits
- The lower limit of cell size is determined by the minimum number of each biomolecule required by the cell
- The upper limit of cell size is determined by the rate of diffusion of solute molecules in aqueous systems
Domains of Life
- Eubacteria: Purple bacteria, Cyanobacteria, Flavobacteria, Thermotoga, Extreme halophiles, Methanogens, Extreme thermophiles
- Archaebacteria
- Eukaryotes: Animals, Ciliates, Fungi, Plants, Flagellates, Microsporidia
- Eubacteria inhabit soils, surface waters, and other living or decaying organisms
- Archaebacterial are more characterized in extreme environments like salt lakes, hot springs, acidic bogs, and ocean depths.
E. coli Cytoplasm
- Contains about 15,000 ribosomes
- Thousands of copies of 1,000 different enzymes
- Numerous metabolites and cofactors
- Variety of inorganic ions
Eukaryotic Cells
- Characterized by the nucleus and various membrane-bound organelles with specific functions (mitochondria, endoplasmic reticulum, Golgi complexes, lysosomes).
Cell Fractionation Techniques
- Differential centrifugation: Separating cellular components based on size and density
- Isopycnic (sucrose-density) centrifugation: Separating components based on density differences in a density gradient
Cytoskeletal Components
- Composed of simple protein subunits
- Polymerize into filaments of uniform thickness
- Undergo constant disassembly and reassembly
Cells Build Supramolecular Structures
- Cells build supramolecular structures from monomeric units
- Macromolecules are assembled into larger complexes
Molecular Components of E. coli
- Key components (Water, Proteins, Nucleic acids, DNA, RNA, Polysaccharides, Lipids, Monomeric subunits, and intermediates, Inorganic ions)
- Approximate percentages of total cell weight
Geometric Isomers
- Maleic acid (cis) and fumaric acid (trans) are geometric isomers that cannot interconvert without breaking covalent bonds
Stereoisomers
- Carbon-containing compounds commonly exist as stereoisomers
- These molecules have the same chemical bonds but differ in stereochemistry
Chiral Centers
- A carbon atom with four different substituents is a chiral center
- Compounds with multiple chiral centers are typically classified using the R/S system
Enantiomers and Diastereomers
- Enantiomers are mirror images of each other
- Diastereomers are non-mirror images
Amino Acids and Sugars
- Amino acids in proteins exist only in their L isomers
- Glucose exists only as its D isomer
Function Groups
- Methyl, Ethyl, Phenyl, Carbonyl, Hydroxyl, Ether, Amido, Amino, Imidazole, Guanidino, Sulhydryl, Disulfide, Thioester, Phosphoryl
Summary of Functional Groups and Biomolecules
- These components define unique properties of biomolecules in living beings
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Description
Test your understanding of the fundamental principles of biochemistry in this quiz focused on Module 1 of the CHY1003 course. Covering topics such as biomolecules, metabolic pathways, and their significance in healthcare, this quiz will help reinforce your knowledge in structural and functional properties of biochemicals. Prepare to evaluate your comprehension of biological chemistry!