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Questions and Answers
What is the primary function of a vortex in a laboratory setting?
What is the primary function of a vortex in a laboratory setting?
- To store liquid samples safely
- To separate liquids by density
- To mix samples at a specific speed and duration (correct)
- To cool down heated samples
Which component of the gel electrophoresis setup is responsible for creating the wells in the gel?
Which component of the gel electrophoresis setup is responsible for creating the wells in the gel?
- The power supply
- The comb (correct)
- The gel photo imaging system
- The box to hold the gel
What role do the positive and negative electrodes play in gel electrophoresis?
What role do the positive and negative electrodes play in gel electrophoresis?
- They keep the gel at a constant temperature
- They create the electrical current needed for separation (correct)
- They photograph the gel after electrophoresis
- They hold the gel in place during the experiment
What device is attached to the cupped rubber piece in a vortex?
What device is attached to the cupped rubber piece in a vortex?
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Which of the following is NOT a major component of gel electrophoresis?
Which of the following is NOT a major component of gel electrophoresis?
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Study Notes
Vortex Mixer
- A simple laboratory tool used to mix small liquid samples.
- Consists of an electric motor connected to a cupped rubber piece.
- Mixing occurs when a test tube or container is pressed against the rubber cup, transmitting motion to the liquid inside.
Gel Electrophoresis
- Separates nucleic acids (DNA, RNA) and proteins based on size and charge.
- Uses an electric current to move molecules through a gel matrix.
- Larger molecules move slower than smaller ones.
Key Components
- Box to hold the gel: Contains buffer solution that conducts electricity.
- Comb: Creates wells in the gel to load DNA samples.
- Positive and negative electrodes: Power supply creates an electric field to pull charged molecules through the gel.
- Power supply: Provides electrical current to run the electrophoresis process.
- Gel photo imaging system: Used to visualize the separated molecules in the gel.
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