Biochemistry Lab Techniques

Genome analysis is the broader field that includes anything that can be done with genome sequences by computational means, while annotation is a subfield that focuses on identifying genes and their functions before analyzing the genome sequence.

To identify the locations of genes and their coding regions in the genome and determine their functions.

Bacterial growth on both LB plates and LB-ampicillin plates.

Bacterial growth on LB plates and few or no colonies on LB-ampicillin plates.

To retain as much of the desired enzyme as possible while removing other proteins, nucleic acid, and contaminants.

Annotation is necessary to make sense of the genome sequence, as it provides valuable information about the genes and their functions.

A comment or explanation attached to text, image, or other data.

Yield or recovery and purification or purification factor.

Differences in the solubility of proteins in aqueous solution containing water-miscible organic solvents.

Water molecules are used up in hydrating the organic solvent molecule, reducing the dielectric constant and removing water of salvation from the protein surface.

To obtain and analyze plasmid DNA, which is essential for understanding the properties and characteristics of plasmids, and to determine their copy number in a bacterial cell.

Due to the charge (ionic) interaction between molecules.

Resistance to antibiotics, production of antibiotics, degradation of complex organic molecules, production of colicins, enterotoxins, restriction and modification enzymes, pigment production, induction of plant tumors, and heavy metal resistance.

Precipitation of proteins.

Low copy number plasmids occur in a limited number, whereas high copy number plasmids occur in a large number, in a bacterial cell.

To separate, analyze, and visualize DNA molecules based on their size and charge.

MALDI TOF is a technique used to analyze proteins by determining their mass-to-charge ratio, which helps in identifying and characterizing proteins.

To understand the instrumentation, experimentation, and interpretation of data obtained using microscopy techniques, such as SEM, TEM, and AFM, to analyze and visualize biological samples.

540

10 minutes

To stop the reaction and measure the absorbance

y = 140.7ln(x) + 70.884

µM/ml/min

As a source of enzyme for the amylase assay

Lambert's law states that when a ray of monochromatic light passes through an absorbing medium, its intensity decreases exponentially as the length of absorbing medium increases.

The Beer-Lambert law states that the intensity of monochromatic light passing through an absorbing medium decreases exponentially as the concentration of the absorbing medium increases.

The molar extinction coefficient (E) is a measure of the absorbance of a compound at a specific wavelength, and it is characteristic of the compound.

The purpose of using a spectrophotometer is to measure the absorbance of the dyes at different wavelengths and plot a graph of absorbance versus wavelength.

The λmax of a given solution is the wavelength at which the solution has maximum absorbance, and it is a characteristic property of the compound.

The importance of using a mixture of two dyes is to demonstrate the ability to characterize a mixture of two colored solutions differently using their absorption spectra.